• Korean journal of applied entomology
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• v.33 no.2
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• pp.66-73
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• 1994

Adult emergence period, the calling behavior pattern and the mating ability of female depending on ages, 9day old male's response to sex pheromone released from each 1- to 5-day old female, and the structure and location of sex pheromone gland were obse~edin Ostrinia furnacalis. Adults usually emerged from 1 hour before to 1-2 hours after the beg~nning of scatophase under the 16L/8D photoperiod. And most active calling behavior was obserued for two hours from the 5th to 7th hours of scotophase among 2- and 3-day old females, and 3-day old male' response to 3-day old females was higher than to any other age of females during the 5th-8th hours of scotophase. Mating frequency was higher at the 4th-7th hours after the beginning of scotophase. Among 1. to Cday old females extruding sex pheromone gland, more than 65% of them successfully mated with 2- to 4-day old males. And, 2- or 3-day old females exhuding sex pheromone gland showed an abdiw. over 60%, to mate with each 1--5-day old males. Hypertrophied cell layers, presumed to be a sex pheromone gland of female adults, were located at two intersegmental membranes between the 8th & 9th, and the 9th & 10th abdominal segments.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.1
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• pp.150-157
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• 2009

This research was performed in order to investigate the anti-inflammatory effects of Bupleuri Radix(BR) on the Immune response in vitro. Cellular proliferation and cytokine production were measured in mast cells or mouse B cells or CD4 Th cells. BR water extract inhibited the secretions of TNF-$\alpha$ and IL-6 in PMA/A23187 stimulated HMC-1 cells. It increased proliferation but did not affect the expressions of CD69 or CD23 in rIL-4/anti-CD40 activated S cells. BR reduced surface IgE expression and secreted IgE but increased the production of IL-4, IFN-$\gamma$ and IgG1 in the same cells. BR caused an increase in proliferation in anti-CD3/anti-CD28 stimulated CD4 Th cells but it did not affect the differentiation of Th1 or Th2 cells. However, IL-2 was increased in BR treated Th2 cells. Considering the above-mentioned results, BR can be applied to a broad range of anti-inflammatory reactions, but our data suggest that it will not be likely to exert any effects on type 1 allergic response.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.15 no.2
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• pp.129-136
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• 1993

The purpose of this study is to evaluate whether the ashed tooth powder is utilized as an alternative material of the implant. For this purpose the author performed the experimental study to investigate the tissue response of sintered toothash and its histocompatibility. Bony defects to expose the body of marrow, $1{\times}1cm$ in size, were created in the right and left mandibular body of mature dog, and then the ashed tooth powders were filled in right side and the blood clot was filled in the left side as an control. The dogs were sacrificed at 4th, 7th, and 16th week after implantation and histologic examination was performed. The results of this study were obtained as follows : 1. Any inflammatory response was not noted after implanting of the ashed tooth powder during the whole experimental period. 2. At 4th week, ashed tooth powders were surrounded by mature connective tissue. And we could observe hydroxyapatite crystal structure within the ashed tooth powder. 3. At 7th week, we could observe that macrophage phagocyted the small granules of ashed tooth powders. 4. At 16th week, the union with host bone by growth of new trabeculae was observed. And there were remnants of ashed tooth power within some of new trabeculae.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.262-269
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• 2005

Kamiinsamyangyoung-tang(KIT) has been widely used to treat amenorrhea and bradymenorrea caused from vital energy and blood deficit. KIT was composed of Insamyangyoung-tang, Cervi Cornu Pantotrichum and Hominis Placenta. The aim of this study is to investigate effect of KIT on Immune response and Blood formation. We investigated thymocytes, splenocytes population, ${\gamma}$-interferon, IL-2, IL-4, NO and phagocytic activity. We found that KIT had no effect on the Th and Tc cell population of thymocytes, Th cell population of splenocytes and ${\gamma}$-interferon quantity was decreased. KIT decreased the formation of Nitric Oxide from abdominal macrophage, on the other hand, it had no influence on the quantity of IL-2, IL-4.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.631-636
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• 2008

Acanthopanax divaricatus var. albeofructus (ADA) have been shown to have various levels of activity such as antioxidant, anticancer, antivirus, and immunostimulatory effects. However, little is known about its mechanism related to the modulation of immune activities. In this study, a water extract of ADA leaves were used to treat human peripheral blood mononuclear cells (hPBMC) to determine the underlying mechanisms for the immunostimulatory effects. To characterize its immunomodulatory activity, the secretion level of various cytokines including IL-2, IL-4, IL-6, IL-10, IL-12, IFN-$\gamma$, and TNF-$\alpha$ were measured using enzyme-linked immunosorbent assay (ELISA). Treatment of hPBMC with ADA leaf extract in an in vitro experiment induced various Th1 cytokines in a dose-dependent manner. A significant increase of IL-2, IL-12, IFN-$\gamma$, and TNF-$\alpha$ secretion was observed in the presence of ADA leaf extract. In contrast, Th2 cytokines including IL-4 and IL-6 were suppressed. There was no significant change in IL-10 release. Our results showed an increase in Th1 and a decrease in Th2 cytokine secretion which suggests that ADA may influence the immune response towards a predominance of Th1 cytokines in the immune system.

• Health Policy and Management
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• v.28 no.1
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• pp.1-2
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• 2018

The Korean Academy of Health Policy and Management (KAHPM) is on its 30th anniversary in 2018. The KAHPM is the first academic society in the field of health policy and management, and it has maintained its significance for 30 years as a representative academic society of health policy and management in Korea. During 30 years, the KAHPM has sought to establish and develop academic field of health policy and management, conducted academic discussions on the agenda of health policy and management in response to the needs of the times and on the health policy issues of the presidential elections, and discussed the healthcare policy of the Government. The 30th anniversary of the KAHPM is scheduled to hold a 30th anniversary academic conference and to prepare the 30th history book of the KAHPM. The offical journal of KAHPM, Health Policy and Management (HPM) will be publishing a special issue with important topics with health policy and management in commemoration of its 30th anniversary. Based on 30 years' experiences, the KAHPM and HPM will be committed to furthering the next 30 years and endeavoring to contribute to improve the quality of life for the people by establishing and practicing desirable health policy and management.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1329-1335
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• 2006

LP-BM5 murine leukemia retrovirus induces the excessive oxidative stress and immune dysfunction leading to B cell leukemia and murine AIDS with cytokine dysfunction. In the present study, the immune restoratory effect of antioxidant hormone dedydroepiandrosterone sulfate (DHEAS) was investigated in the primary splenocytes from LP-BM5 retrovirus-infected C57BL/6 mice. DHEAS significantly increased T and B cell response to mitogen and normalized the unbalanced production of Th1/Th2 type cytokines. In particular, both protein and mRNA expression of IL-4, IL-6, and $TNF-\alpha$ were down-regulated by DHEAS treatment whereas IL-2 and $IFN-\gamma$ level were increased. This result suggests that DHEAS directly or indirectly regulates the gene expression of Th1/Th2 type cytokines in transcription level. In addition, DHEAS treatment decreased the hepatic lipid peroxidation and preserved vitamin E level in liver cells. These results suggested that DHEAS could effectively prevent immune dysfunction by regulating cytokine secretion and preventing the oxidative stress in murine AIDS.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.5
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• pp.439-448
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• 2021

DA-9601 is an extract obtained from Artemisia asiatica, which has been reported to have anti-inflammatory effects on gastrointestinal lesions; however, its possible anti-inflammatory effects on the small intestine have not been studied yet. Therefore, in this study, we investigated the protective effects of DA-9601 against the ACF-induced small intestinal inflammation. Inflammation of the small intestine was confirmed by histological studies and the changes in the CD4⁺ T cell fraction induced by the inflammation-related cytokines, and the inflammatory reactions were analyzed. Multifocal discrete small necrotic ulcers with intervening normal mucosa were frequently observed after treatment with ACF. The expression of IL-6, IL-17, and TNF-α genes was increased in the ACF group; however, it was found to have been significantly decreased in the DA-9601 treated group. In addition, DA-9601 significantly decreased the levels of proinflammatory mediators such as IL-1β, GM-CSF, IFN-γ, and TNF-α; the anti-inflammatory cytokine IL-10, on the other hand, was observed to have increased. It is known that inflammatory mediators related to T cell imbalance and dysfunction continuously activate the inflammatory response, causing chronic tissue damage. The fractions of IFN-γ⁺ Th1 cells, IL-4⁺ Th2 cells, IL-9⁺ Th9 cells, IL-17⁺ Th17 cells, and Foxp3⁺ Treg cells were significantly decreased upon DA-9601 treatment. These data suggest that the inflammatory response induced by ACF is reduced by DA-9601 via lowering of the expression of genes encoding the inflammatory cytokines and the concentration of inflammatory mediators. Furthermore, DA-9601 inhibited the acute inflammatory response mediated by T cells, resulting in an improvement in ACF-induced enteritis.

• Journal of Microbiology and Biotechnology
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• v.26 no.3
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• pp.469-476
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• 2016

This study was undertaken to evaluate the immunomodulatory effect of dead nano-sized Lactobacillus plantarum (nLp) in RAW 264.7 cells and murine primary splenocytes. nLp is a dead, shrunken, processed form of L. plantarum nF1 isolated from kimchi (a traditional Korean fermented cabbage) and is less than 1 μm in size. It was found that nLp treatment stimulated nitric oxide (NO) production more in RAW 264.7 macrophages than pure live L. plantarum (pLp), and that the stimulatory properties were probably largely derived from its cell wall. In addition, nLp induced murine splenocyte proliferation more so than pLp; in particular, a high dose of nLp (1.0 × 10¹¹ CFU/ml) stimulated proliferation as much as lipopolysaccharide at 2 μg/ml. Moreover, according to our cytokine profile results in splenocytes, nLp treatment promoted Th1 (TNF-α, IL-12 p70) responses rather than Th2 (IL-4, IL-5) responses and also increased Th17 (IL-6, IL-17A) responses. Thus, nLp stimulated NO release in RAW 264.7 cells and induced splenocyte proliferation more so than pLp and stimulated Th1 and Th17 cytokine production. These findings suggested that dead nLp has potential use as a functional food ingredient to improve the immune response, and especially as a means of inducing Th1/Th17 immune responses.

• YAKHAK HOEJI
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• v.56 no.6
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• pp.366-371
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• 2012

In this present study, we determined whether or not there is an immunoadjuvant effect of ochnaflavone, a biflavone isolated from Lonicera japonica. As an antigenic source, the cell wall (CACW) of Candida albicans, a fungal pathogen, was used. CACW consists of 95% carbohydrate (mannan). In the experiments, BALB/c mice were immunized with emersion forms of CACW combined with or without ochnaflavone (Och) in the presence of IFA containing mineral oil or CACW alone. Then, the amounts of antisera collected from these mice groups were measured by the ELISA method. Data from these experiments showed that CACW combined with Och (CACW/Och/IFA) provoked the production of antisera app. 2.2 or 5 times more than the corresponding CACW/IFA or CACW alone (CACW/DPBS), respectively, in mice (P<0.05). We further examined the immune response type induced by Och. Analysis of the values of the IgG1/IgG2a ratios obtained from IgG isotyping revealed that Och induced Th2-immunity more dominantly than Th1. This finding was confirmed by cytokine profile. CACW/Och/IFA formulation induced IL-4 (Th2-type cytokine) more than IFN${\gamma}$ (Th1-type cytokine) as compared with CACW/IFA and CACW/DPBS formulations (P<0.05). All data combined, Och appears to have an immunoadjuvant activity that may convert Th1 immunity into Th2 immunity.