• Research in Plant Disease
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• v.24 no.3
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• pp.193-201
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• 2018

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes bacterial soft rot on a wide range of crops worldwide, especially in countries with warm and humid climates. This study was conducted to establish an efficient screening method for resistant cultivars of radish (Raphanus sativus) to bacterial soft rot. Resistance degrees of 60 commercial radish cultivars to the Pcc KACC 10421 isolate were investigated. For further study, six radish cultivars (Awooriwoldong, YR Championyeolmu, Jeonmuhumu, Bitgoeunyeolmu, Sunbongaltari, Housecheongok) showing different level of resistance to the bacterium were selected. The development of bacterial soft rot on the cultivars was tested according to several conditions such as incubation temperature, seedling stage of radish, inoculum concentration to develop the disease. On the basis of the results, we suggest that an efficient screening method for resistant radish to Pcc is to inoculate twenty-day-old seedlings with a bacterial suspension of Pcc at a concentration of $8{\times}10^5cfu/ml$ and then to cultivate the plants in a growth room at $25^{\circ}C$ and 80% RH with 12-hour light per day.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.2
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• pp.187-201
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• 1996

This study was to evaluate the efficiency of diffusive monitor using activated carbon fiber(ACF, KF-1500) in measuring airborne organic solvents. The following characteristics were identified and studied as critical to the performance of diffusive monitor; recovery, sampling rate, face velocity, reverse diffusion and storage stability. For the evaluation of the performance of this monitor, MIBK, PCE, toluene were used as organic solvents. In the sampling rate experiments, eight kinds of solvents (n-hexane, MEK, DIBK, MCF, TCE, CB, xylene, cumene) as well as the above solvents were used. The results were as follows: 1. The desorption efficiencies(DE's) of ACF diffusive monitor ranged from 83 % to 101 %. In contrast, those of coconut shell charcoal ranged from 78 % to 102 %. Especially, the DE's of ACF for the polar solvents such as MEK were superior to those of charcoal. 2. Experimental sampling rates on ACF were average 42ml/min(37-46ml/min) for 11 organic solvents at $24{\pm}2^{\circ}C$, $50{\pm}5%RH$. However ideal sampling rates(DA/L) were 33 % higher than experimental sampling rates. 3. The initial response(15~16 min) of the testing monitor was 2 times higher than the actual concentration determined by the reference methods at $24{\pm}2^{\circ}C$, $8{\pm}5%RH$ and $80{\pm}5%RH$. Within 1 hours, the curve reached a linear horizontal line at low humidity condition. But sampling efficiencies decreased with respect to time at high humidity condition. And sampling efficiencies were higher at high humidity condition than low humidity condition for MIBK. 4. At very low velocity (less than 0.02 m/sec), the concentration of ACF diffusive monitor were poorly estimated. But ACF diffusive monitor were not affected at higher velocity(0.2 m/sec-0.6 m/sec). 5. There was no significant reverse diffusion when the ACF monitors were exposed to clean air for 2 hours after being exposed for 2 hours at the level of 1 TLV. 6. There was no significant sample loss during 3 weeks of storage at room temperature and 5 weeks of storage at refrigeration.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.652-663
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• 1996

The purpose of this study was to evaluate the tensile bond strength to tooth structure of composite resin and glass ionomer cement according to filling methods and light curing units. In this study, two class V cavities were prepared on the buccal surface of each tooth of 140 extracted human molars, and they were randomly assigned into 3 experimental groups with 40 teeth and control group with 20 teeth. And then, each experimental groups subdivided into 2 groups(A,B) according to light curing units. The cavities of each group were filled with the CLEARFIL FII self curing resin(Control Group), Z-100 light curing resin(Group 1), Vitremer$^{TM}$ light curing glass ionomer cement(Group 2) and Z-100 light curing resin over the Vitrebond$^{TM}$ liner(Group 3). And subdivided A Group used Argon Laser(SPECTRUM$^{TM}$, U.S.A.), B Group used XL 1,000 curing light (3M, U.S.A.). The specimens underwent temperature changed from $5^{\circ}C$ to $55^{\circ}C$ five hundred times. After thermocycling, specimens were stored in 100% relative humidity at $37^{\circ}C$ for 24 hours. And then, the tensile bond strength of specimens were calculated with Universal Testing Machine(AGS-100A, Japan). The results were as follows : 1. Among the experimental groups, the group 2-B showed the highest tensile bond strength ($18.89{\pm}7.80$) and the group 1-A showed the lowest tensile bond strength ($11.68{\pm}2.28$). There was significant difference between group 2-B and group 1-A(p<0.01). 2. Between the light curing units, the XL 1,000 unit showed higher tensile bond strength ($16.63{\pm}3.20$) than that of the Argon Laser unit ($13.73{\pm}2.30$). There was significant difference between XL 1,000 and Argon Laser(p<0.01). 3. About filling methods and materials, the group 2 showed the highest tensile bond strength ($17.56{\pm}1.89$) and the group 1 showed the lowest tensile bond strength($13.03{\pm}1.90$). There was significant difference between group 2 and group 1,3(p<0.01). In conclusion, the results showed that the glass-ionomer cement that cured by XL 1,000 light curing unit demonstrated significantly higher tensile bond strength than other curing unit and filling methods.

• Restorative Dentistry and Endodontics
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• v.35 no.6
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• pp.486-491
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• 2010

Objectives: This study examined the effect of 2% chlorhexidine on the ${\mu}TBS$ of a direct composite restoration using one-step self-etch adhesives on human dentin. Materials and Methods: Twenty-four extracted permanent molars were used. The teeth were assigned randomly to six groups (n = 10), according to the adhesive system and application of chlorhexidine. With or without the application of chlorhexidine, each adhesive system was applied to the dentin surface. After the bonding procedure, light-cure composite resin buildups were produced. The restored teeth were stored in distilled water at room temperature for 24 hours, and then cut and glued to the jig of the microtensile testing machine. A tensile load was applied until the specimen failed. The failure mode was examined using an operating microscope. The data was analyzed statistically using one-way ANOVA, Student's t-test (p < 0.05) and Scheffet's test. Results: Regardless of the application of chlorhexidine, the Clearfil $S^3$ Bond showed the highest ${\mu}TBS$, followed by G-Bond and Xeno V. Adhesive failure was the main failure mode of the dentin bonding agents tested with some samples showing cohesive failure. Conclusions: The application of 2% chlorhexidine did not affect the ${\mu}TBS$ of the resin composite to the dentin using a one-step self-etch adhesive.

• Journal of the Korean GEO-environmental Society
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• v.15 no.2
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• pp.27-36
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• 2014

Due to the latitudinal location of Korea, the seasonally frozen ground has been focused on as research topics such as the frost heaving under the asphalt road rather than the permafrost ground. However, the recent construction of the second Korean Antarctic research station, the Jangbogo station and the participation on the development of the natural gas pipeline in Russia arouse the research interests on the behavior of the permafrost ground. At the design process of the geotechnical structures on the permafrost ground, the evaluation of the creep characteristics of the frozen soil is very crucial. Since the domestic specification on the frozen soil testing does not exist currently, it is necessary to evaluate the creep characteristics of frozen soils systematically with regard to the affecting factors. Therefore, the creep characteristics of the frozen specimens of dense Jumoonjin sand were evaluated under various loads at -5 and $-10^{\circ}C$. Based on the test results, as the load became close to the strength and the temperature became lower, the duration of the secondary creep became shorter and more distinct tertiary creep responses were observed.

• Journal of Powder Materials
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• v.28 no.2
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• pp.143-149
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• 2021

In this study, (GaN)_1-x(ZnO)_x solid solution nanoparticles with a high zinc content are prepared by ultrasonic spray pyrolysis and subsequent nitridation. The structure and morphology of the samples are investigated by X-ray diffraction (XRD), field-emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The characterization results show a phase transition from the Zn and Ga-based oxides (ZnO or ZnGa₂O₄) to a (GaN)_1-x(ZnO)_x solid solution under an NH3 atmosphere. The effect of the precursor solution concentration and nitridation temperature on the final products are systematically investigated to obtain (GaN)_1-x(ZnO)_x nanoparticles with a high Zn concentration. It is confirmed that the powder synthesized from the solution in which the ratio of Zn and Ga was set to 0.8:0.2, as the initial precursor composition was composed of about 0.8-mole fraction of Zn, similar to the initially set one, through nitriding treatment at 700℃. Besides, the synthesized nanoparticles exhibited the typical XRD pattern of (GaN)_1-x(ZnO)_x, and a strong absorption of visible light with a bandgap energy of approximately 2.78 eV, confirming their potential use as a hydrogen production photocatalyst.

• Tunnel and Underground Space
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• v.31 no.6
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• pp.400-414
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• 2021

Under high-level radioactive waste repository conditions, bentonite as an engineered barrier material undergoes thermal, hydrological, mechanical, and chemical processes. We report the applications of X-ray Computed Tomography (CT) imaging technique on the characterization and analysis of bentonite over the past decade to provide a reference of the utilization of this technique and the recent research trends. This overview of the X-ray CT technique applications includes the characterization of the bentonite either in pellets or powder form. X-ray imaging has provided a means to extract grain information at the microscale and identify crack networks responsible for the pellets' heterogeneity. Regarding samples of pellets-powder mixtures under hydration, X-ray CT allowed the identification and monitoring of heterogeneous zones throughout the test. Some results showed how zones with pellets only swell faster compared to others composed of pellets and powder. Moreover, the behavior of fissures between grains and bentonite matrix was observed to change under drying and hydrating conditions, tending to close during the former and open during the latter. The development of specializing software has allowed obtaining strain fields from a sequence of images. In more recent works, X-ray CT technique has served to estimate the dry density, water content, and particle displacement at different testing times. Also, when temperature was added to the hydration process of a sample, CT technology offered a way to observe localized and global density changes over time.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.683-691
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• 2021

The purpose of this study is to measure the yield and to evaluate the physiological activity of Cannabis sativa seed(Hemp seed) extracts extracted using a density fluctuation supercritical carbon dioxide for each temperature condition-30℃(HSSE30), 45℃(HSSE45), 60℃(HSSE60), and to enable dissolution of the poorly water-soluble extracts by liposome formulation and to enhance the skin permeability. As a result of the yield measurement, HSSE60 showed the highest yield, and in the antioxidant activities, HSSE45 had the highest total polyphenol content, and showed the highest DPPH, ABTS+ radical scavenging activities at the highest concentration of the extracts. As a result of the antimicrobial susceptibility testing, a clear zone appeared only in the Propionibateium acnes strain. It was confirmed that particle size was reduced and the absolute value of the zeta potential increased in the case of the formulation in which the extracts were in liposomes than in the formulation in which the extracts were dissolved in deionized water, and the skin permeability was improved. Based on these experimental results, we confirmed the possibility of using the hemp seed supercritical carbon dioxide extracts, a poorly water-soluble extract, can be applied as a functional natural material for cosmetics.

• Journal of Food Hygiene and Safety
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• v.38 no.4
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• pp.246-254
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• 2023

V. parahaemolyticus causes waterborne and foodborne disease such as acute diarrhea. In this study, V. parahaemolyticus isolates from seawater, fish tanks, and distributed fishery products in Jeju were investigated for potential toxin or species-specific genes (tdh, trh, tlh, and toxR) using RT-PCR and their genetic characteristics were analyzed using Pulsed-field gel electrophoresis (PFGE). Overall, V. parahaemolyticus of 90 strains (36.7%), including 33 strains from seawater, 8 strains from fish tanks, and 50 strains from fishery products, were isolated from 245 samples. All V. parahaemolyticus strains did not detect the tdh gene, whereas all strains detected tlh or toxR genes. In addition, trh genes were detected in 3 strains from seawater and 1 strain from fishery products. Monthly quantitative testing of seawater revealed that V. parahaemolyticus was positively correlated with water temperature. The 90 strains of V. parahemolyticus obtained in this study showed by gene homology between types, ranging from 64.0-97.3%. Among these, thirteen types showed 100% homology between genes. These results indicate that continuous monitoring is needed to facilitate food poisoning epidemiological investigations because some isolated V. parahaemolyticus strains harbored toxin genes and V. parahaemolyticus strains isolated from seawater, fish tanks, and distributed fishery products showed genetic similarity.

• Analytical Science and Technology
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• v.19 no.4
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• pp.290-300
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• 2006

Disinfection by-products(DBPs), such as volatile trihalomethanes and the nonvolatile organochlorine acids, created by chlorination have been extensively studied. However MX which contributes 20-50% of the mutagenic activity in drinking water began to people's attention since 1990. Its chemical name is 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone. According to WHO guidelines its concentration should be controlled, but its value has not been set up. Due to analytical difficulties in measuring this compound at such a low concentrations and lack of information on toxicity to human. Because concentration (ng/L) of MX in drinking water is low traditional testing methods are ineffective. Therefore this study compared LLE and SPE and have chosen SPE to improve preconcentration. MX has been identified in chlorinated drinking water samples in several countries but not in korea Therefore this study analyzed concentration of MX in different water sources and in spring water. This study examined the causes of changing MX content. Chlorine dosage, seasons, water temperature and distance from the source was all discoverd to be relavant. MX was analyzed in various treatment to find optimum disinfection methods. The outcome was that the concentration of MX was minimized when using biological activated carbon-O3 and granular activated carbon.