• Title/Summary/Keyword: Testes

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Stereological study of testes following experimentally-induced unilateral cryptorchidism in rats

  • Javad Sadeghinezhad;Fatemeh Yarmahmoudi;Mohammad Mehdi Dehghan;Saeed Farzad Mohajeri;Ehsan Roomiani;Hadis Bojarzadeh;Mahdi Aghabalazadeh Asl;Ava Saeidi;Margherita De Silva
    • Clinical and Experimental Reproductive Medicine
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    • v.50 no.3
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    • pp.160-169
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    • 2023
  • Objective: Cryptorchidism is one of the main causes of infertility and can result in testicular cancer. This study aimed to present quantitative data on the damage caused by cryptorchidism using stereological analysis. Methods: Thirty newborn rats were randomly divided into control and experimental groups. The experimental group underwent surgery to induce unilateral cryptorchidism in the left testis, whereas the control group underwent a sham surgical procedure 18 days after birth. The testes were removed at designated time points (40, 63, and 90 days after birth) for stereological evaluation and sperm analysis. Total testicular volume, interstitial tissue volume, seminiferous tubule volume and length, and seminiferous epithelium volume and surface area were measured. Other parameters, such as sperm count, sperm morphology, and sperm tail length, were also examined. Results: Statistically significant differences (p<0.05) were observed between the experimental and the control groups at different ages regarding the volumes of various parameters, including the surface area of the germinal layer, the length of the seminiferous tubules, sperm count, and sperm morphology. However, no significant differences were observed in the epithelial volume and the sperm tail length of the groups. Conclusion: Given the substantial effect of cryptorchidism on different testicular parameters, as well as the irreversible damage it causes in the testes, it is important to take this abnormality seriously to prevent these consequences.

A Study on Physico-chemical Property and Composition of Velvet Antler and Byproducts in Elk. (Elk종의 녹용과 부산물의 이화학적 특성에 관한 연구)

  • 이길왕
    • Journal of Life Science
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    • v.14 no.3
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    • pp.496-500
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    • 2004
  • This study has been performed for physico-chemical property and composition of sinew, testes, tail, blood and velvet antler in Elk. Amino acid contents in the sinew taurine, alanine, histidine, and lysine were high contained, histidine, glutamic acid, taurine, and lysine were high contained in testes, glutamic acid, lysine, alanine, glycine, and phenylalanine were high contained in tail, histidine, glycine, and lysine were high contained in blood, glutamic acid, lysine, taurine, alanine, and glycine were high contained in velvet antler. And, based on the amount of mineral, $K^{+}$, $Ca^{2+}$, and $P^{+}$ were 444.8, 166.6 and 242.9mg per 100g in sinew, respectively. $K^{+}$, $Ca^{2+}$ and $P^{+}$ were 294.4, 330.5, and 514.3 mg per 100g in testes, respectively, $K^{+}$, $Mg^{2+}$, and $P^{+}$ were 1420.6, 118.4, and 1105.2mg per 100 mg in tail, respectively. Fe3+,-K+, and P+ were 344.1, 1023.6 and 157.2 mg per 100 mg in blood, respectively and $K^{+}$, $Ca^{2+}$ and $P^{+}$ were 888.4, l1533.1 and 14722.0 in velvet antler, respectively. Finaly, difference were found in comparison of composition of free mwtal ion (N $a^{+}$, $K^{+}$, $Mg^{2+}$ and $Ca^2$) bwteen blood, tail, tests, sinew and velvet antler, and composition of free metal in blood higher than those othersd higher than those others.

Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes

  • Kim, In-Hye;Kim, Si-Kwan;Kim, Eun-Hye;Kim, Sung-Won;Sohn, Sang-Hyun;Lee, Soo-Cheol;Choi, Sang-Dun;Pyo, Suhk-Neung;Rhee, Dong-Kwon
    • Journal of Ginseng Research
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    • v.35 no.3
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    • pp.272-282
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    • 2011
  • Ginseng (Panax ginseng Meyer) has been shown to have anti-aging effects in animal and clinical studies. However, the molecular mechanisms by which ginseng exerts these effects remain unknown. Here, the anti-aging effect of Korean red ginseng (KRG) in rat testes was examined by system biology analysis. KRG water extract prepared in feed pellets was administered orally into 12 month old rats for 4 months, and gene expression in testes was determined by microarray analysis. Microarray analysis identified 33 genes that significantly changed. Compared to the 2 month old young rats, 13 genes (Rps9, Cyp11a1, RT1-A2, LOC365778, Sv2b, RGD1565959, RGD1304748, etc.) were up-regulated and 20 genes (RT1-Db1, Cldn5, Svs5, Degs1, Vdac3, Hbb, LOC684355, Svs5, Tmem97, Orai1, Insl3, LOC497959, etc.) were down-regulated by KRG in the older rats. Ingenuity Pathway Analysis of untreated aged rats versus aged rats treated with KRG showed that the affected most was Cyp11a1, responsible for C21-steroid hormone metabolism, and the top molecular and cellular functions are organ morphology and reproductive system development and function. When genes in young rat were compared with those in the aged rat, sperm capacitation related genes were down-regulated in the old rat. However, when genes in the old rat were compared with those in the old rat treated with KRG, KRG treatment up-regulated C21-steroid hormone metabolism. Taken together, Cyp11a1 expression is decreased in the aged rat, however, it is up-regulated by KRG suggesting that KRG seems enhance testes function via Cyp11a1.

Feeding Effect of an Anabolic Steroid, Nandrolone, on the Male Rat Testis

  • Lee, Dong-Mok;Min, Tae-Sun;Choi, In-Ho;Cheon, Yong-Pil;Chun, Tae-Hoon;Park, Chang-Sik;Lee, Ki-Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.12
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    • pp.1566-1577
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    • 2010
  • Nandrolone, 19-nortestosterone, is a synthetic androgenic-anabolic steroid promoting muscle growth. Nandrolone is also present in pig meat and sera at non-negligible levels. A number of scientific reports have suggested a positive relationship between incidence of infertility and increased meat consumption in humans. The present study was designed to determine out the effect of feeding nandrolone on the testis of the male reproductive tract. Mixtures of food and nandrolone at different concentrations (0.005 ppm and 0.5 ppm) were supplied to pubertal male rats for 6 weeks. Body weight was recorded every week during the entire experimental period. At the end of the treatment, the testis, epididymis, and epididymal fat were collected and weighted. Sperm numbers in the caudal epididymis were counted. Differential gene or protein expression of steroidogenic enzymes in the testes among experimental groups was determined by semi-quantitative real-time PCR or western blotting analysis, respectively. Histological changes of the testis induced by nandrolone treatment were examined by hematoxylin and eosin staining. Immunohistochemical analysis was employed to detect changes in the localization of steroidogenic enzymes in the testes among experimental animals. There were no significant changes on body, testis, epididymis, and epididymal fat weights among experimental groups. A significant increase of caudal sperm number was found in the 0.5 ppm nandrolone-treated group. Histological examination of the testes noted a high frequency of germ cell sloughing in seminiferous tubules of 0.5 ppm nandrolone-treated rats. Even though transcript levels of $3{\beta}$-hydroxysteroid dehydrogenase (HSD) I, $17{\beta}$-HSD4, and $17{\alpha}$-hydroxylase were influenced by nandrolone treatments, protein levels of all molecules examined in the present study were not significantly affected. Immunohistochemical analysis showed no visible changes in the localization of steroidogenic enzymes in the testes among experimental groups. The current study showed that oral intake of nandrolone in male rats for 6 weeks did not cause significant damage to the testis. It is considered that a feeding effect of nandrolone on male fertility would not be remarkable.

The necessary & method of scrotoplasty in female to male transgender (여성에서 남성으로의 성전환증에서 음낭성형술의 필요성과 방법)

  • Kim, Seok-Kwun;Moon, In-Sun;Kwon, Yong-Seok;Lee, Keun-Cheol
    • Archives of Plastic Surgery
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    • v.36 no.4
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    • pp.437-444
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    • 2009
  • Purpose: Transgender is a disorder of gender identity, who have appropriate chromosomal, hormonal and anatomical characteristics corresponding to their sexual phenotype but feel strongly with respect to their sexual identity, that they belong to the opposite sex. There is a persistence discomfort and sense of inappropriateness about one's assigned sex in a person who has reached puberty. Transgender is a psychiatric problem, but surgical method provides more satisfactory adjustment for patients. In gender reassignment surgery for female to male transgender, mastectomy, nipple reduction, hysterectomy, oophorectomy and phalloplasty are included. And as the final operation, recommended for scrotoplasty and artificial testes insertion. So we investigated the necessity and method of scrotoplasty in the final operation of female to male transgender. Method: The authors have long term follow-up of 75 cases female to male transgender during January, 1991 to February, 2008. Among them, 13 cases were evaluated in this study. During phalloplasty, the labium major skin preserved. And this labium majoral skin flap was made for the neoscrotum. At least six months later, artificial testes were inserted in neoscrotum with local anesthesia. Middle sized (3 cm diameter) artificial testes(silicon gel or carving soft silicone implant) were used because of the limitation of the neoscrotum. We evaluated the questionnaire and interview about the postoperative satisfaction in configuration of reconstructed scrotum, and the necessity of operation, the postoperative psychosocioeconomic improvement and limitation of body exposure activities such as swimming, public bathing. Results: Based on this study, satisfaction of reconstructed scrotum after scrotoplasty was improved(92%). The necessity of scrotoplasty was in 92.3% and the postoperative psychosocioeconomic well - being improvement was 77% in answers. Less limitation of activities requiring body exposure was 54% in answers. Most of the patients were satisfied with the results of surgical operation inspite of the operative procedure had some postoperative complications. Conclusion: This study was reported that the scrotoplasty in female to male transgender is not only a conversion of external genitalia but also an improvement of psychosocial state. Most patients sincerely hope to this operation, so we improve our surgical method for more good results.

An Ultrastructural Study of Sertoli Cells in Human Fetal Testes (태아 고환에서 버팀세포의 미세형태학적 연구)

  • Lee, Tae-Jin;Yoon, Sam-Hyun;Kim, Mi-Kyung;Park, Eon-Sub;Yoo, Jae-Hyung
    • Applied Microscopy
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    • v.31 no.2
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    • pp.157-165
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    • 2001
  • Sertoli cells in the normal adult testis are nondividing cells, which are relatively inconspicuous on cross section of the seminiferous tubule and comprise about 10% to 15% of the tubular cellular elements. Ultrastructurally, Sertoli cells have characteristic nucleoli, plasma membrane, and cytoplasmic components. The plasma membrane has two types of intercellular junctions which are developed at puberty: junctions between adjacent Sertoli cells and Sertoli cell-germ ceil junction. However, the ultrastructural findings of Sertoli cells in human fetus is not fully elucidate yet. In the present study, human fetal testes ($14\sim27$ weeks) obtained from artificially induced abortions legally without gross malformation were studied using transmission electron microscopy to make clear the differentiation process of Sertoli cells in human. In human fetal testes from 14 weeks to 27 weeks, the cell junctions of Sertoli-germ cells and Sertoli-Sertoli cells are desmosome like structure and not tight junction or desmosome. The Overall intracytoplasmic organelles of Sertoli cells are relatively sparse. The mitochondrias are relatively abundant but no developed cristae. And the rough endoplasmic reticuli are abundant and smooth endoplasmic reticuli are sparse. The amount of lipid droplets are regularly observed in human fetal Sertoli cells. No microfilaments or Charcot-Bottcher's crystalloids are present. From the results, Sertoli cells in human fetal testes are somewhat different ultrastructural findings with puberty or adult. However, to make clear the differentiation process of Sertoli cells in human, further study for 28 weeks to puberty is required.

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Morphological Differentiation of Leydig Cells in Human Fetal Testes (사람 태아 고환에서 간질세포 분화의 형태학적 관찰)

  • Rhee, Kye-Il;Kim, Dae-Joong;Kim, Kyung-Yong;Park, Eon-Sub
    • Applied Microscopy
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    • v.29 no.4
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    • pp.511-522
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    • 1999
  • The Leydig cell found within the interstitium of the testis is important in the spermatogenesis. The differentiation of Leydig cell, even though relatively well known in animals, is not fully elucidated yet in human. In the present study, human fetal testes ($14\sim27$ weeks) obtained from artificially induced abortions legally without gross malformations were studied using light and transmission electron microscopy to make clear the differentiation process of Leydig cells in humans. Leydig cells could be classified as 4 types: fusiform, light, dark, and degenerating cells. The most immature cell was the fusiform cells found frequently at 14 weeks, which seemed to differentiate successively into light and dark cells. Light cells were most frequently found at 17 weeks and dark cells at 24 weeks. Light cells were found to have mitochondria and smooth endoplasmic reticuli (sER) most prominently than any other cell types. The lumen of sER became to be expanded with age. Some electron-dense inclusions were observed in the mitochondrial matrix of the dark cells. Lipid droplets found more in light cells than dark cells were most prominent at 16 weeks and gradually decreased after 20 weeks. Glycogen particles were rich in dark cells. Degenerating cells were most frequently found at 27 weeks. From the results, it is suggested that Leydig cells in human fetal testes undergo similar differentiation process af in animals.

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Effect of Di-(2-ethylhexyl)phthalate(DBHP) on Spermatogenesis in Rat Testes (흰쥐 정자형성과정에 미치는 Di-(2-ethylhexyl)phthalate의 영향)

  • 김완종;길영천;이종화;신길상
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.285-292
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    • 1999
  • Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer known as one of endocrine disruptors. The present study was carried out to investigate the alterations of function and ultrastructure in rat testes after oral intubation of DEHP in dosages of 1g/kg/day, 2 g/kg/day or 3 g/kg/day in 0.5 ml of corn oil for 15 days. DEHP reduced the growth of body and testes, inhibited apermatogenesis and induced structural changes on various cell types of the rat testis. Leydig cells, Sertoli cells and the developing germ cells seemed to be impaired their differentiations in terms of the structural changes of cell organelles. The increase of heterochromatin in amount were common features in all 3 cell types. In addition, the Leydig cells were characterized by the swelling of smooth endoplasmic reticulum and perinuclear space, the increases in number and size of Iysosomes. The Sertoli cells became irregular in nuclear envelope and the number of Iysosomes and vacuoles seemed to be increased. There were some indications of necrosis of the germ cells, such as vacuolized nucleus and segregated nucleolus. And also, DEHP lowered the level of testosterone in experimental rat serum. DEHP suppressed apermatogenesis decreasing developing germ cells and these effects of DEHP on the rat testis were dose dependent. The detrimental effect of DEHP on apermatogenesis and ultrastructure of rat testes seems to be derived from the decreased level of testosterone by Leydig cells, followed by the abnomalities of Sertoli cells and the germ cells.

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