• BMB Reports
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• 제34권1호
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• pp.59-65
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• 2001

In subtractive hybridization, target sequences in the tester are enriched by hybridizing with an excess amount of driver, followed by removing the tester hybridized with the driver. All of existing subtractive cloning methods are designed to remove the tester/driver hybrid. The removal of hybrid, however, is often unsatisfactory For various reasons. In this study we developed a subtractive enrichment protocol in which the tester/driver can be completely removed by selecting only the tester/tester after hybridization. In this protocol both the tester and driver DNAs are ligated with same linker DNAs and amplified by polymerase chain reaction (PCR). The tester DNA is then digested with two different enzymes and used in subsequent hybridization with an excess driver. After hybridization, the DNA is ligated with the adaptor that is only compatible with the tester/tester. Since only the tester/tester can have the new adaptor, no tester/driver can be amplified by PCR in this protocol. Unlike other methods, a 100% subtraction efficiency can be achieved even though the enzymatic treatments used in the enrichment procedure are incomplete. Furthermore, only the hybridized tester DNA can have the new adaptor and be amplified by PCR, resulting in 100% denaturation in effect. The efficacy of this novel method was verified with the model system in which a known amount of the target sequence is included.

• 한국안전학회지
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• 제24권2호
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• pp.42-47
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• 2009

The flash point and the AIT(auto-ignition temperature) are the most important combustible properties used to determine the potential for the fire and explosion hazards of flammable material. In order to know the accuracy of data in MSDS(Material Safety Data Sheet), the flash point of n-acids were measured by using Pensky-Martens closed cup tester(ASTM D93), Setaflash closed cup tester(ASTM D3278), Tag open cup tester(ASTM D1310) and Cleveland open cup tester(ASTM D92). Also, the AIT of n-acids were measured by using ASTM E659-78 tester. The measured the flash points and the AIT were compared with literatures and MSDS in KOSHA. The measured the flash points and the AIT were different from those in literatures and MSDS. Therefore, This paper shows that it is needed to investigate the MSDS compatibility of n-acids for the fire safety objectives.

• Journal of Information Processing Systems
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• 제4권4호
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• pp.121-132
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• 2008

VLSI chips have been tested using various automatic test equipment (ATE). Although each ATE has a similar structure, the language for ATE is proprietary and it is not easy to convert a test program for use among different ATE vendors. To address this difficulty we propose a tester structure expression language, a tester language with a novel format. The developed language is called the general tester language (GTL). Developing an interpreter for each tester, the GTL program can be directly applied to the ATE without conversion. It is also possible to select a cost-effective ATE from the test program, because the program expresses the required ATE resources, such as pin counts, measurement accuracy, and memory capacity. We describe the prototype environment for the GTL and the tester selection tool. The software size of the prototype is approximately 27,800 steps and 15 manmonths were required. Using the tester selection tool, the number of man-hours required in order to select an ATE could be reduced to 1/10. A GTL program was successfully executed on actual ATE.

• 수산해양교육연구
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• 제28권2호
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• pp.350-355
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• 2016

This research aims to evaluated the structure improvement and new product development of valve tester. A valve tester was redesigned for structure improvement and new product development using 3-D design program CATIA. In addition, behavior analysis was conducted on the modeled valve tester using the ANSYS program. The total deformation, stress and strain were obtained by the internal pressure change. This result was applied to the new product development of valve tester.

• 조명전기설비학회논문지
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• 제15권6호
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• pp.58-64
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• 2001

본 논문은 LVI시험기 및 모델 변압기의 설계와 제작에 관하여 기술하였다. 이들을 이용하여 LVI 시험 분석기 술을 개발하였다. LVI시험기는 본테, 터미널 박스 및 케이블로 구성하였고, ANSI규격에서 요구하는 출력특성을 갖는 것으로 평가되었다. 모델변압기는 탭들을 만들어 변압기의 권선변형 모델을 만들 수 있도록 하였고, LVI시험기로써 실험을 실시하여 LVI시험분석 기술에 관하여 논하였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2009년도 춘계학술대회 논문집
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• pp.1661-1666
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• 2009

This paper is analyzed the effect on characteristics of thrust force of linear induction motor(LIM) by arc type tester. Many kinds of tester have a rotational shape because of a finite length of railroad. Whereas effects by using rotational type tester are generally unknown. For reason of that, this paper is analyzed the effect on characteristics by rotational type tester using 2D finite element method(FEM), and then is compared a thrust force between linear type tester and rotational type tester. Analysis model is decided by numerical modeling for fitting the LIM for propulsion system of light rail, and it is changed to proper arc model. And then we analyzed the arc and the linear model. Consequently, arc type tester is not only useful experiment but also efficient simulation method, and small error is occurred between linear and arc model.

• 청정기술
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• 제11권3호
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• pp.153-164
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• 2005

This study found a reliable evaluation for four kinds of pb-Free plating solutions and it's layers, through pure Sn, SnAg, SnBi and SnCu. These four kind of solutions are widely used to pb-Free plating. Hull-cell, Harring-blum, coverage, throwing power, current efficiency, stability, life-time, composition, hardness, roughness, abrasion, scratch, solderability, corrosion, contact angle, morphology, SIR(Surface insulation resistance) and Whisker test were experimented. Also, Using ICP, XRF, FE-SEM, EDS, temperature/humidity chamber, solderability tester, hardness tester, roughness tester, abrasion tester, salt spray tester, contact angle tester, SIR tester, and microscope. In this paper could be shown the systematic and various analysis for reliability about four kinds of pb-Free plating solutions, processes and it's deposit surface.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.643-644
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• 2008

This paper is analyzed the effect on characteristics of thrust force of linear induction motor(LIM) by rotational type tester. Many kinds of tester have a rotational shape because of a finite length of railroad. Whereas effects by using rotational type tester are generally unknown. For reason of that, this paper will be analyzed the effect on characteristics by rotational type tester using 2D finite element method(FEM), and then will be compared a thrust force between linear type tester and rotational type tester.

• 한국자동차공학회논문집
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• 제12권4호
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• pp.101-109
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• 2004

Three models with various degree-of-freedom for a manual transmission clutch full-size tester have been developed and the models' reliability and accuracy have been verified using the measured data. A simulation study has also been conducted to understand dynamic behavior of the tester. The model for this simulation study includes clutch disk friction and damper dynamics. The developed model is very accurate in terms of maximum torque exerted on the clutch, slip duration and the vibration response except a slight difference compared to the measured data. In a history graph of the clutch torque, the maximum torque response from simulation is flat but the measured is sunken with a noticeable curvature. This phenomenon is found to be irrelevant to the dynamics of the full-size tester but is originated from the characteristics of the clutch itself. Thus, the full-size tester has been proven to be a reliable tester for clutch's power and torque transmission capability. To obtain a better understanding of clutch's characteristics and relationship between full-size tester and other testing methodologies, future research directions have been suggested.

• 한국화재소방학회논문지
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• 제21권3호
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• pp.56-60
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• 2007

세계적으로 GHS와 REACH의 도입으로 위험물의 위험성평가를 위한 기존의 MSDS 이용이 증가하여 MSDS의 정확도를 알아보기 위하여 n-Decanol의 인화점을 Tag closed tester, Seta-flash closed tester, Pensky-Martens closed tester, Cleveland open tester 기기를 이용하여 국내와 일본에서 각각 측정하였다. 실험은 국내와 일본에서 각각 다른 제조사에서 구입한 시약으로 인화점을 측정하였으며 인화점 값은 국내와 일본에서 유사한 값을 얻었으나 기존의 MSDS와 문헌의 인화점 값과 비교하여 차이가 크다는 것이 발견되었다. 본 연구에서는 국내와 일본에서 측정된 시험값의 타당성을 제시하고 제조업체가 MSDS를 작성할 시는 신중을 기하여 작성하여야 하며 GHS와 REACH를 위한 위험물분류시는 신뢰성 있는 기관에서 발행한 레포트를 참고로 할 것을 제시하였다.