• Journal of Veterinary Clinics
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• v.27 no.1
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• pp.62-65
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• 2010

Arowana (Scleropages formosus) is the most valuable group of ornamental fishes and very much in demand in the ornamental fish trade and commands high price ranging from hundreds to thousands of dollars per fish. In this paper, we described a case of mortality of arowana from a private aquarium in Korea. A bacterial pathogen from fish organs (brain, kidney, liver) was cultured, identified and confirmed using Vitek System 2, API 20E test, multiplex PCR and 16S rRNA gene sequencing. The morphological and biochemical properties of the bacterium isolated from the brain, kidney and liver of the fish were similar to Aeromonas sobria. Positive amplification products using the multiplex PCR assay for detection of A. sobria were obtained from these organs. The 16S rRNA gene of the isolates from fish was identical and exhibited 100% sequence similarity with A. sobria (AY987762.1) strain available from GenBank. This bacterium contained hemolysin gene, a virulence factor that plays an important role in outbreaks of disease and is pathogenic to humans as well as in fish. Although this opportunistic bacterium was isolated from a fish without any external symptoms, this pathogen may act as a reservoir and enhance chances of zoonosis to human such as during handling.

• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.290-301
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• 1990

The 167 plus tree clones of Pinus koraiensis in clone bank planted in 1983 were investigated for time of flowering, rate of flowering, and number of flowering from 1986 to 1989. The results were as follows : 1) There were clones in the minority which do not cross in natural pollination between earlest flowering female clones and latest shedding male clones. 2) The rate of male flowering appeared less than rate of female flowering and received influence of genetic more than rate of female flowering. 3) The numbers of flowering in female and male flower were affected by small number of clones. 4) The flowering number for female strobili was not related to the flowering number of male flower. The 42 clones among 167 clone had not male flower. 5) The flowering number of 167clones were classified three groups in female flower and four groups in male flower by L.S.D 5% test. 6) The clones with abundant female and male flower were selected based on the component analysis. 7) As Based on flowering rate and number of female, the juvenile phase of plus tree clones appeared to be until four years after grafting ; the transition period appeared to be from five to seven years after grafting ; the adult phase appeared to begin from eight years after grafting, 8) The grafted trees of Pinus koraiensis appeared early flowerring about six years compared with seedling trees.

• Journal of Chest Surgery
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• v.34 no.4
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• pp.305-310
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• 2001

배경: 판막대치술에 냉동보존판막의 이용은 감염에 대한 저항성과 탁월한 혈류역학으로 증가하고 있다. 판막육아세포의 생존율은 이식된 냉동보존 판막의 내구성에 영향을 미친다고 알려져 있고, 세포의 생존율은 warm ischemic time에 영향을 받는 것으로 알려져 있다. 냉동 보존하여 이식할수 있는 공여 판막의 warm ischemic time 의 적정치를 구하기 위하여, warm ischemic time에 다른 세포의 생존율을 관찰하였다. 대상 및 방법: 1.조직의 획득: 실제 판막을 냉동 보존하는 상황과 유사하게 하기 위하여 도살된 돼지의 심장과 폐를 밀봉한 상태로 4~8$^{\circ}C$로 냉장 보관하여 (warm ischemic time) 일정시간이 경과한 후, 심장과 폐에서 심장을 적출하여 4$^{\circ}C$하트만 용액에 24시간 보관하였다.(cold ischemic time). Warm ischemic time에 따라 2시간, 12시간, 24시간 36시간으로 4군으로 나누었으며, 각 군마다8개의 돼지 심자을 이용하였다. 2. 조직의 멸균: RRMI 1640에 항생제를 섞은 용액에 멸균하고, 3 냉동과 냉동보존; American tissue bank에서 제시한 냉동곡선에 따라 냉동하여, 액체질소 탱그에서 7일간 보존 후 해동하였다. 4. 생존율의 측정; 판막의 생존율 검사는 Triphan blue test로 하였고, 각각 warm ischemic period후 , cold ischemic period 후, 해동 후에 시행하였다. 5. 분석방법; 분석은 SAS program의 pearson correlation으로 하였다. 결과: 1. 멸균, 냉동과 냉동 보존하는 과정의 적합성을 규명하기 위하여 이 과정의 전과 후인 Cold ischemic period 후와 해동 후의 대동맥판막의 생존율의 차이를 비교한 결과, 차이가 없었다.(p =0.619). 2, warm ischemic time 과 warm ischemic period 후 , Cole ischemic period 후와 해동후의 대동맥판막의 생존율과의 correlation 은 각각 R= -0.857, -.0.673과 -0.549로 강하거나 , 혹은 뚜렷한 음성적 관계가 있었다. 삼천판막의 생존율과 대동맥판막의 생존율과 뚜렷한 상관관계가 있었다. 결론; 1. Warm ischemic time 이 길어지면 판막유아세포의 생존율이 감소하고, 12시간 이상되면 해동후의 판막육 아페포의 생존율이 50% 이하로 떨어졌다. 2. 본 연구에서 시행한 판막의 냉동보존방법은 세포의 생존율을 유지하는데 양호한 것으로 나타났으며 삼천판막으로 대동맥판막의 생존율을 예측해 볼 수 있다. 3. 그러나, 이식후 장기간 적절한 내구성을 갖기 위한 이식될 판막의 생존율은, 육아세포에 관한 여구가 좀 더 되어야 규명될 것이다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.601-610
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• 2021

The tidal current patterns at Keum River Estuary before and after the construction of coastal structures were compared according to the CASES. The depth-integrated and tidal difference treatment applied FLOW2DH numerical model was used for the tidal current predictions. The test conditions consisted of before construction of coastal structures (CASE1), after construction of coastal structures (CASE2), and the addition of watergate operation(CASE1Q and CASE2Q), and present (CASE3). CASE1 showed a stable tidal current pattern, such as a natural estuary. In CASE2, the tidal current velocities and directions of the Keum River Estuary were changed due to the installed coastal structures. In particular, the tidal current velocities of the Gaeya open channel sections (P5~P9) in CASE2 were calculated to be 10~30% larger than that of CASE1. In the case of the Gunsan Inner Harbor (P4), which is closest to the Geum River Estuary, the ebb flow rate was approximately 250~300% faster than that of other CASEs due to the discharge of the watergate operation for 2.7 hours during the ebb of CASE1Q and CASE2Q. This will affect sediment transport, and it is predicted to lead to seabed changes. CASE3 is considered to be entering the stabilization stage according to the simulation of the tidal current velocities and directions of the Keum River Estuary and the surrounding coastal area.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.544-552
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• 2019

The purpose of this study was to find concrete measures to improve the subjective health level, happiness and quality of life of the elderly according to economic level and to propose social and policy alternatives accordingly. As a research method, 63,929 elderly people aged 65 or older were surveyed using the Community Health Survey (Indicator Bank) _v09, and the frequency of health use by economic level, subjective health level, euphoria and quality of life Analysis and Chi square analysis and independent t-test. Multi variate logistic regression analysis was performed with subjective health level as a dependent variable and multiple linear regression analysis was performed to determine the factors affecting euphoria and quality of life. The results of the study are as follows. In the case of recipients, medical use was lower than that of non-recipients, lower education level, female age of 75 years or older, and less stress, In case of present or past recipients, the result of non - receipt increased as the subjective health level was worse, and the non - recipient had higher euphoria and quality of life. As a result, there is a need for alternatives to increase opportunities for medical use among the recipients, with particular attention being paid to women and elderly people over 75 years old. It is expected to be used as a basic data to effectively improve the health promotion, happiness and quality of life of the elderly people of low income group.

• International Area Studies Review
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• v.15 no.2
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• pp.125-150
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• 2011

As the economy grows, there is a concern that the economic development causes a productivity reduction because of the service intensive growth. However, the economy of developed countries encounter with the productivity growth as their economy grows, which phenomenon called Baumol's paradox. Oulton (1999, 2001) find out the reason of Baumol's paradox in a forward and backward chain effects. So, this paper is aimed at verifying the theory of Oulton (1999, 2001). Moreover, we test the difference effect between a consumer service and producer service input using a dummy variable. We use the Input-Output Table (1990, 1995, 2000, 2005) that is offered by the Bank of Korea to accomplish the purpose of our research that is represented above. We find out that the Korea's intermediate producer service inputs cause a multifactor productivity growth. That result is matched with the Oulton (1999, 2001)'s theory. But, the intermediate consumer service inputs don't have a significant effect on a multifactor productivity. The result of verifying the effect of intermediate producer service inputs among industries shows that the effects on manufacture industries are less than other industries.

• Korean Journal of Poultry Science
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• v.45 no.4
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• pp.237-244
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• 2018

In this study, to increase the survival rate of frozen/thaw rooster semen, standard protocols of semen thawing procedures were tested by computer-assisted sperm assay (CASA). We tested 4 different thawing protocols for frozen semen, $5^{\circ}C$ for 2 min, $35^{\circ}C$ for 30 s, $54^{\circ}C$ for 13 s, and $70^{\circ}C$ for 7 s. The pooled semen from 5 to 8 Ogye rooster line was diluted in the HS-1 diluent and frozen in 8% methylacetamide (MA) in liquid nitrogen vapors. To determine standard thawing method, straws were plunged into different temperatures and times. The resulting motilities were recorded by the CASA system. The results of this study showed that the best viability of the spermatozoa was shown by exposure at $5^{\circ}C$ for 2 min. Moreover, the longevity test of thawed sperm at $5^{\circ}C$ for 2 min also supported the higher viability under low temperature preservation of $17^{\circ}C$ for 1 hr. Further research is needed to increase the motility of thawed rooster semen for field application. In addition, the in vivo tests for different rooster lines are also needed for the establishment of avian genetic resource bank.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.64-64
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• 2019

Screening and identification of genetic resources based on their phytoconstituents and morphological characters potentially provide baseline data for researchers, breeders, and nutraceutical companies who wish to formulate a nutrient-dense diet and health beneficial supplement. Thus, we evaluated the amount of total phenolic content and major phenolic compounds; examined if phenolic compounds could be used as distinguishing factors for perilla genetic resources; and investigated the relation between some quantitative and qualitative morphological characters with the contents of phenolic compounds in 360 accessions obtained from National Agrobiodiversity Center gene bank, Jeonju, Korea. Total phenolic content (TPC) was estimated using Folin-Ciocalteu colorimetric assay. Individual phenolic compounds were determined using an Ultra-High Performance Liquid Chromatography system equipped with Photodiode Array detector. Considerable variations were observed in TPC (7.99 to 117.47 mg GAE/g DE), rosmarinic acid (RA) (ND to 19.19 mg/g DE), caffeic acid (CA) (ND to 0.72 mg/g DE), apigenin-7-O-diglucuronide (ADG) (ND to 1.24 mg luteolin equivalent (LUE)/g DE), scutellarein-7-O-glucuronide (SG) (ND to 4.32 mg LUE/g DE), and apigenin-7-O-glucuronide (AG) (ND to 1.60 mg LUE/g DE). RA was the most dominant phenolic compound in most accessions (95.3%) followed by SG. The adaxial leaf color was light green, green and dark green in 13.8%, 65.0%, and 21.1 % of the accessions, respectively. 78.8% of the accessions had light green color at the abaxial side with the remaining being described as green. Most of the accessions (96.9%) were cordate shape, the remaining being eclipse. Intensities of green pigment at abaxial and adaxial leaf surfaces were correlated with contents of individual phenolic compounds and TPC whereas leaf length and width had no correlation with TPC, CA and RA, and negatively correlated with ADG, AG, and SG. Leaf shape was not related with content of phenolic compounds, color of leaves, or the length or width of leaves. Accessions IT57426, IT157434, IT267710, and IT267712 which contained relatively high contents of TPC and major phenolic compounds (RA and SG) could be used for further research in breeding and bioassay test. Our study result showed the contents of total phenolics and individual phenolic compounds along with the morphological characters could be useful distinguishing factors for perilla genetic resources.

• Clinical and Experimental Pediatrics
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• v.64 no.12
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• pp.632-641
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• 2021

Background: Over the past few decades, various goals have been defined to reduce the mortality of children caused by acute lower respiratory infections (ALRIs) worldwide. However, few spatial studies to date have reported on ALRI deaths. Purpose: We aimed to assess the spatial modeling of mortality from ALRI in children under 5 years of age during 2000-2017 using a global data. Methods: The data on the mortality of children under 5 years old caused by ALRI were initially obtained from the official website of the World Health Organization. The income status of their home countries was also gathered from the Country Income Groups (World Bank Classification) website and divided into 5 categories. After that, in the ArcGIS 10.6 environment, a database was created and the statistical tests and related maps were extracted. The Global Moran's I statistic, Getis-Ord Gi statistic, and geographically weighted regression were used for the analyses. In this study, higher z scores indicated the hot spots, while lower z scores indicated the cold spots. Results: In 2000-2017, child mortality showed a downward trend from 17.6 per 100,000 children to 8.1 and had a clustered pattern. Hot spots were concentrated in Asia in 2000 but shifted toward African countries by 2017. A cold spot that formed in Europe in 2007 showed an ascending trend by 2017. Based on the results of geographically weighted regression test, the regions identified as the hot spots of mortality from ALRI in children under 5 years old were among the middle-income countries (R²=0.01, adjusted R²=8.77). Conclusion: While the total number of child deaths in 2000-2017 has decreased, the number of hot spots has increased among countries. This study also concluded that, during the study period, Central and Western Africa countries became the main new hot spots of deaths from ALRI.

• Economic Analysis
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• v.27 no.1
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• pp.67-90
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• 2021

This study found an interesting fact that the nonlinear relationship structure between volatility and trading volume changed before and after the COVID-19 pandemic according to empirical analysis using Bitcoin (BTC) market data that sensitively reflects investors' trading behavior. That is, their relationship appeared positive (+) in a stable market state before COVID-19 pandemic, as in theory based on the information flow paradigm. In a state under severe market stress due to COVID-19 pandemic, however, their dependence structure changed and even negative (-). This can be seen as a consequence of increased market stress caused by COVID-19 pandemics from a behavioral economics perspective, resulting in structural changes in the asset market and a significant impact on the nonlinear dependence of volatility and trading volume (in particular, their dependence at extreme quantiles). Hence, it should be recognized that in addition to information flows, psychological phenomena such as behavioral biases or herd behavior, which are closely related to market stress, can be a key in changing their dependence structure. For empirical analysis, this study performs a test of Ross (2015) for detecting a structural change, and proposes a Copula Regression Quantiles (CRQ) approach that can identify their nonlinear relationship structure and the asymmetric dependence in their distribution tails without the assumption of i.i.d. random variable. In addition, it was confirmed that when the relationship between their extreme values was analyzed by linear models, incorrect results could be derived due to model specification errors.