• International Journal of Contents
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• v.14 no.2
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• pp.1-6
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• 2018

In a wireless mobile communication system, a pilot signal has been considered to be a necessary signal for estimating a changing channel between a base station and a terminal. All mobile communication systems developed so far have a specification for transmitting pilot signals. However, although the pilot signal transmission is easy to estimate the channel,(Ed: unclear wording: it is easy to use the pilot signal transmission to estimate the channel?) it should be minimized because it uses radio resources for data transmission. In this paper, we propose a pilotless channel estimation scheme (PCE) by introducing the clustering method of unsupervised learning used in our deep learning into channel estimation.(Ed: highlight- unclear) The PCE estimates the channel using only the data symbols without using the pilot signal at all. Also, to apply PCE to a real system, we evaluated the performance of PCE based on the resource block (RB), which is a resource allocation unit used in LTE. According to the results of this study, the PCE always provides a better mean square error (MSE) performance than the least square estimator using pilots, although it does not use the pilot signal at all. The MSE performance of the PCE is affected by the number of data symbols used and the frequency selectivity of the channel. In this paper, we provide simulation results considering various effects(Ed: unclear, clarify).

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.294-295
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• 2007

80년대 초에 개발된 CD(Compact Disc) Disc는 지난 27여 년간 급속한 발전을 하였다. 95년에 DVD가 발표되면서 영화 Title제작이 되어 우리는 안방에서 고화질의 DVD를 감상할 수 있었다. CD-R(Recordable) 로부터 시작된 기록 기술은 CD-RW를 거쳐 DVD-Recordable, DVD-ReWritable 및 DVD-RAM, Light Scribe등 거쳐 BD(Blu-Ray) 기술에 까지 이르게 되었다. 광 Disc 기술은 3가지 분야로 나눌 수 있다. 안정된 제어를 위한 Servo 기술, Mechanical 기술, PC와communication 할 수 있는 Interface 기술 등으로 나눌 수 있다. Servo 제어 기술은 다음과 같이 4가지 분야로 구성되어 있다. Focus Servo, Tracking Servo, Sled Servo, Spindle Servo. Disc 회전을 제어를 담당하고 있는 Spindle Servo Block에서는 Hall Sensor를 이용하여 Disc의 회전을 Count하여 Feedback 하였다. 그러나 갈수록 심화 되고 있는 가격 경쟁 상황에서 Hall Sensor를 대체하여 회전수를 Conut하는 방법이 필요 하였다. BEMF(역기전력)을 이용한 Zero Crossing 방법이 이를 대체 하였지만, Motor의 전기적인 특성 편차 등에 안정된 control이 안되어서, Motor를 관리하는 방법으로 진행되어 왔다. 이것은 생산 Loss로 이어져서 Hall Sensorless의 장점을 훼손하게 되었다..본 논문에서는 이러한 Sensorless Type Spindle Motor의 문제점에 대해 Common 단자를 이용하여 안정된 제어 방법을 제안 한다. less Type의 문제점인 초기 기동 문제를 Common 단자를 이용하여 안정되게 Spindle motor를 Control 하는 방법에 대해 연구 하고자 한다.

• Journal of Microbiology
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• v.33 no.3
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• pp.201-207
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• 1995

We have previously reported that SPP2 gene product of yeast Saccharomyces cerevisiae is involved in the pre-mRNA splicing. To investigate the rol ein the splicing pathway of the Spp2p protein, the SPP2 gene was overexpressed in Escherichia coli and polyclonal antibodies to Spp2p were generated from rabbits. First, a DNA fragment containing the SPP2 GENE without its promoter was subcloned into an E. coli expression vector, pKK233-3. The resulting recombinant plasmid pBQ14 contained an IPTG inducible tac promoter and the SPP2 structural gene. Overexpression of the SPP2 gene was achieved by additionof 0.1 to 1.0 mM IPTG to a logarithmic culture of E. coli JM103(pBQ14) for 90 min at 37.deg.C. Sequence of N-terminal 15 amino acids of the overproduced protein was well matched to the deduced one from the SPP2 reading frame. Then, polyclonal antibodies were generated from rabbits immunized with gel-purified SppSp protein. These antibodies reacted specifically with the Spp2p protein extracted from yeast cells expressing the SPP2 gene to a great extent. The antibodies could also block the activity of yeast splicing extracts.

• ETRI Journal
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• v.43 no.2
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• pp.232-245
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• 2021

As wireless communication continues to develop in limited frequency resource environments, it is becoming important to identify the state of spectrum utilization and predict the amount needed in future. It is essential to collect reliable information for data analysis. This paper introduces a platform that enables the gathering of the scheduling information of a long-term evolution (LTE) cellular system without connecting to the network. A typical LTE terminal can confirm its assigned resource information using the configuration parameters delivered from a network. However, our platform receives and captures only the LTE signal over the air and then enables the estimation of the data relevant to scheduling for all terminals within an LTE cell. After extracting the control channel signal without loss from all LTE subframes, it detects valid downlink control information using the proposed algorithm, which is based on the error vector magnitude of depatterned symbols. We verify the reliability of the developed platform by comparing it with real data from mobile phones and service operators. The average difference in resource block utilization is only 0.28%.

• Journal of Korea Society of Digital Industry and Information Management
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• v.14 no.4
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• pp.79-85
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• 2018

This paper presents an implementation method of Long Term Evolution-Advanced (LTE-A) Physical Downlink Shared Channel (PDSCH) decoder using a general-purpose multicore Digital Signal Processor (DSP), TMS320C6670. Although the DSP provides some useful coprocessors such as turbo decoder, fast Fourier transformer, Viterbi Coprocessor, Bit Rate Coprocessor etc., it is specific to the base station platform implementation not the mobile terminal platform implementation. This paper shows an implementation method of the LTE-A PDSCH decoder using programmable DSP cores as well as the coprocessors of Fast Fourier Transformer and turbo decoder. First, it uses the coprocessor supported by the TMS320C6670, which can be used for PDSCH implementation. Second, we propose a core programming method using DSP optimization method for block diagram of PDSCH that can not use coprocessor. Through the implementation, we have verified a real-time decoding feasibility for the LTE-A downlink physical channel using test vectors which have been generated from LTE-A Reference Measurement Channel (RMC) Waveform R.6.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.113.1-113
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• 2003

A potexvirus, Hosta virus X (HVX-Kr), causing mosaic and mottle symptoms was isolated from hosta plants (Hosta spp.), and its entire genome RNA sequence was determined. in Korea using cDNA library and RACE methods. The genome of HVX encodes five open reading frames coding for viral replicase, triple gene block (TGB), and viral coat protein (CP) from the 5'to 3' ends, which is a typical genome structure of potexviruses. The 3-terminal region of the virus includes the TGBI (26 kDa), TGB2 (13 kDa), TGB3 (8 kDa), and 23 kDa coat protein (CP) and the 3-nontranslated region (NTR). The CP gene of the type isolate of HVX (HVX-U) was amplified by RT-PCR and its nucleotide sequence was determined. The CPs of HVX-Kr and HVX-U had 100％ and 98.9％ identical amino acids and nucleotides, respectively. Most of the regions of the genome HVX had over 50％ nucleotide identical to other sequenced potexviruses. This is the first report of complete genome sequence information of HVX and molecular evidence supporting the virus as a distinct species of the genus Potexvirus.

• Polymer(Korea)
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• v.34 no.3
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• pp.253-260
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• 2010

In this study, we prepared and evaluated a series of biocompatible and biodegradable block copolymer hydrogels with a delayed swelling property for tissue expander application. The hydrogels were synthesized via a radical crosslinking reaction of poly(ethylene glycol) (PEG) diacrylate and poly(D,L-lactide-co-glycolide)-poly(ethylene glycol)-poly(D,L-lactide-co-glycolide)(PLGA-PEG-PLGA) triblock copolymer diacrylate as a swelling/degradation controller (SDC). For the synthesis of various SDCs that can lead to different degradation and swelling properties, various PLGA-PEG-PLGA triblock copolymers with different LA/GA ratios and different PLGA block lengths were synthesized and modified to have terminal acrylate groups. The resultant hydrogels were flexible and elastic even in the dry state. The in vitro degradation tests showed that the delayed swelling properties of the hydrogels could be modulated by varying the chemical composition of the biodegradable crosslinker (SDC) and the block ratio of SDC/PEG. The histopathologic observation after implantation of hydrogels in mice was performed and evaluated by macrography and microscopy. Any significant inflammation or necrosis was not observed in the implanted tissues. Due to their biocompatibility, elasticity, sufficient swelling pressure, delayed swelling and controllable degradability, the hydrogels could be useful for tissue expansion and other biomedical applications.

• BMB Reports
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• v.30 no.5
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• pp.303-307
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• 1997

Grb2, which is composed of a Src homology 2 (SH2) domain and two Src homology 3 (SH3) domains, is known to serve as an adaptor protein in signaling for Ras activation. Thus, a blocker of the Grb2 interactions with other proteins can be a potential candidate for an anticancer drug. In this study, we have developed a high throughput screening method for SH3 domain binding ligands and blockers. Firstly, we made and purified the glutathione S-transferase (GST)-fusion proteins with the Grb2 SH2 and SH3 domains, and the entire Grb2. This method measures the binding of a biotin-labeled oligopeptide, derived from a Grb2/SH3 binding motif in the hSos, to the GST-fusion proteins, which are precoated as glutathione S-transferase fusion protein on a solid phase. When $1\;{\mu}g$ of each fusion protein was used to coat the wells, both N- and C- terminal SH3 the domains as well as the whole of Grb2 were able to interact with the biotin-conjugated ligand peptide, while the SH2 domain and GST alone showed no binding affinity. Although N- and C- terminal SH3 domains showed an increase of binding to the ligand peptide in proportion to the amount of peptide, the GST fusion protein with Grb2 demonstrated much higher binding affinity. GST-Grb2 coating on the solid phase showed a saturation curve; 66 and 84% of the maximal binding was observed at 100 and 300 ng/$100\;{\mu}l$, respectively. This binding assay system was peptide sequence-specific, showing a dose-dependent inhibition with the unlabeled peptide of SH3 binding motif. Several other peptides, such as SH2 domain binding motifs and PTB domain binding motif, were ineffective to inhibit the binding to the biotin-conjugated ligand peptide. These results suggest that our method may be useful to screen for new anticancer drug candidates which can block the signaling pathways mediated by SH3 domain binding.

• Journal of the Korean Chemical Society
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• v.5 no.1
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• pp.33-37
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• 1961

We have shown that carboxy-peptidase destroys the biological activity of angiotensin octa-and deca-peptides. Since Proline occurs as the seventh amino acid from the amino end of the chain and since carboxypeptidase does not cleave proline from a peptid chain, it is evident that the heptapeptid H.asp-arg-val-tyr-ileu-his-pro.OH is formed by this hydrolysis. This peptide must then be biologically inactive. In order to determine whether the phenyl group of the C-terminal amino acid was the necessary requirement for biological activity of the octapeptide, $ala^8$ angiotensin octapeptide(amino acids of peptides numbered from amino end) was synthesized. For this synthesis the four dipeptides were prepared: carbobenzoxy-L-prolyl-L-alanine-P-nitrobenzyl-ester, m.p. $134-135^{\circ}C,$ carbobenzoxy-L-isoleucyl-imidazole benzyl-L-histidine methyl ester, m.p. $114-116^{\circ}C,$ carbobenzoxy-L-valyl-L-tyrosine hydrazide and carbobenzoxy B-benzyl-L-aspartyl-nitro-L-arginine. The first three dipeptides were obtained as crystalline compounds. Imidazole-benzyl-L-histidine was used in the hope that it would block the histidine imidazole against side reactions in steps subsequent to the formation of the C-terminal tetrapeptide. Also, it was through that the imidazole benzylated peptides would be easier to crystallize. This, however, was not the case. The tetrapeptide, carbobenzoxy-L-isoleucyl-L-im, benzyl-histidyl, L-prolyl-L-alanine-nitrobenzyl ester was not obtained in a crystalline form. Neither could the mono-or dihydrobromide of the tetrapeptide free base be induced to crystallize. Carbobenzoxy-L-valyl-L-tyrosine azide was condensed with the tetrapeptide free base to yield the protected hexapeptide; carbobenzoxy-L-valyl-L-tyrosyl-L-isoleucyl-L-im, benzyl, histidyl-L-Prolyl-L-alanine-nitrobenzyl ester. Upon removal of the carbobenzoxy group with hydrogen bromide in acetic acid an amorphous free base hexapeptide ester was obtained. This compound gave the correct C, H, N analysis and contained the six amino acids in the correct ratio. The octapeptide was obtained by condensing this hexapeptide with carbobenzoxy-B-benzyl-L-aspartyl-nitro, L-arginine using the mixed anhydride method of condensation. This amorphous product was proven to be homogenous by chromatography in two solvent systems and upon hydrolysis yielded the eight amino acids in correct ratio. The five protecting groups were removed from the octapeptide by hydrogenolysis over palladium black catalyst. Biological assay of the free peptide indicated that it possessed less than 0.1 per cent of both pressor and oxytocic activity of the phenylalanine8 angiotensin. This suggests that the phenyl group is a point of attachment between angiotensin and its biological receptor site.

• Journal of Navigation and Port Research
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• v.31 no.3 s.119
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• pp.165-172
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• 2007

Logistics needs in Asia are surprisingly increasing due to rapid growth of China in Northeast Asia We are endeavoring to make an economic block in opposition to NAFTA and EU by economic revival, political stability, and easing of exclusive parts between countries as increasing of traffic infra such as asian highway, transcontinental railway in the region of Northeast Asia and as considering diversification of transportation mode. Now, Busan is not a final terminal of this business, and it is not a concreted level in considering an undersea tunnel between Korea and Japan as effort to extend to Japan. Therefore, this study performed an effect analysis which be affected to Northeast Asia and Korea in future by analysis and comparison of a cost and time required of marine transportation and railway before and after construction through course analysis and summary of discussed content until now for undersea tunnel between Korea and Japan. This result expects as a accelerative play for study cf undersea tunnel between Korea and Japan in future. In this result, we estimate that it is an effective things in a cost and time required if undersea tunnel between Korea and Japan is constructed, and we simply searched for theirs effects in the aspects of Northeast Asia, national balanced development, regional economic in Busan, and marine logistics.