• Journal of Chest Surgery
• /
• v.23 no.3
• /
• pp.452-464
• /
• 1990

It is well known that extracellular Calcium plays a very important role in several steps of smooth muscle excitability and contractility, and there have been many concerns about factors influencing the distribution of extracellular Ca++ and the Ca++ flux through the cell membrane of the smooth muscle. Based on the assumption that Mg++ may also play an important role in the excitation and contraction processes of the smooth muscle by taking part in affecting Ca++ distribution and flux, many researches are being performed about the exact role of Mg++, especially in the vascular smooth muscle. But yet the effect of Mg++ in the smooth muscle activity is not clarified, and moreover the mechanism of Mg++ action is almost completely unknown. Present study attempted to clarify the effect of Mg++ on the excitability and contractility in the multiunit and unitary smooth muscle, and the mechanism concerned in it. The preparations used were the guinea-pig aortic strip as the experimental material of the multiunit smooth muscle and the rat uterine strip as the one of the unitary smooth muscle. The tissues were isolated from the sacrificed animal and were prepared for recording the isometric contraction. The effects of Mg++ and Ca++ were examined on the electrically driven or spontaneous contraction of the preparations. And the effects of these ions were also studied on the K+ or norepinephrine contracture. All experiments were performed in tris-buffered Tyrode solution which was aerated with 100% 02 and kept at 35oC. The results obtained were as follows: 1] Mg++ suppressed the phasic contraction induced by electrical field stimulation dose-dependently in the guinea-pig aortic strip, while the high concentration of Ca++ never recovered the decreased tension. These phenomena were not changed by the a - or b - adrenergic blocker. 2]Mg++ played the suppressing effect on the low concentration [20 and 40 mM] of K+-contracture in the aortic muscle, but the effect was not shown in the case of 100mM K+-contracture. 3] Mg++ also suppressed the contracture induced by norepinephrine in the aortic preparation. And the effect of Mg++ was most prominent in the contracture by the lowest [10 mM] concentration of norepinephrine. 4] In both the spontaneous and electrically driven contractions of the uterine strip, Mg++ decreased the amplitude of peak tension, and by the high concentration of Ca++ the amplitude of tension was recovered unlike the aortic muscle. 5] The frequency of the uterine spontaneous contraction increased as the [Ca++] / [Mg++] ratio increased up to 2, but the frequency decreased above this level. 6] Mg++ decreased the tension of the low[20 and 40mM] K+-contracture in the uterine smooth muscle, but the effect did not appear in the 100mM K+-contracture. From the above results, the following conclusion could be made. 1] Mg++ seems to suppress the contractility directly by acting on the smooth muscle itself, besides through the indirect action on the nerve terminal, in both the aortic and uterine smooth muscles. 2] The fact that the depressant effect of Mg++ on the K+-contracture is in inverse proportion to an increase of K+ concentration appears resulted from the extent of the opening state of the Ca++ channel. 3] Mg++ may play a depressant role on both the potential dependent and the receptor-operated Ca++ channels. 4] The relationship between the actions of Mg++ and Ca++ seems to be competitive in uterine muscle and non-competitive in aortic strip.

• Geomechanics and Engineering
• /
• v.11 no.2
• /
• pp.253-267
• /
• 2016

The stability of surrounding rock will be poor when the tunnel is excavated through nearly horizontal stratum. In this paper, the instability mechanism of local nearly horizontal stratum in super-large section and deep buried tunnel is revealed by the analysis of the macro failure and micro fracture. A structural model is proposed to explain the mechanics of surrounding rock collapse under the action of stress redistribution and shed light on the macroscopic analytical approach of the stability of surrounding rock. Then, some highly effective formulas applied in the tunnel engineering are developed according to the theory of mixed-mode micro fracture. And well-documented field case is made to demonstrate the effectiveness and accuracy of the proposed analytical methods of mixed-mode fracture. Meanwhile, in order to make the more accurate judgment about yield failure of rock mass, a series of comprehensive failure criteria are formed. In addition, the relationship between the nonlinear failure criterion and $K_I$ and $K_{II}$ of micro fracture is established to make the surrounding rock failure criterion more comprehensive and accurate. Further, the influence of the parameters related to the tension-shear mixed-mode fracture and compression-shear mixed-mode fracture on the propagation of rock crack is analyzed. Results show that ${\sigma}_3$ changes linearly with the change of ${\sigma}_1$. And the change rate is related to ${\beta}$, angle between the cracks and ${\sigma}_1$. The proposed simple analytical approach is economical and efficient, and suitable for the analysis of local nearly horizontal stratum in super-large section and deep buried tunnel.

• Journal of Korean Society of Steel Construction
• /
• v.19 no.1
• /
• pp.67-78
• /
• 2007

In this study, we performed an investigation on the variations in the structural capacity of steel plate walls with various infill plate details. Five three-story plate walls with thin web plates were tested. Parameters for the test specimens were the connection details between the moment frame and infill plates, such as weld and bolt connections, the location and length of weld connection, and coupling wall. Regardless of the details of infilled steel plate, the steel plate wall specimens showed excellent initial stiffness, strength, and energy dissipation capacity. However, the wall with bolt-connected infill plates showed slightly low deformation capacity. This result showed that for workability and cost efficiency,various wall details can be used in practice without causing a significant decrease in the structural capacity of steel plate walls. A method for making projections on strength and energy dissipation capacity of steel plate wall specimens with various details was developed.

• Journal of Veterinary Clinics
• /
• v.22 no.3
• /
• pp.220-227
• /
• 2005

Although ketamine has been used in the field of anesthetic medicine for its safety and favourable respiratory effects, the cardiovascular effects of ketamine is still controversial. To clarify the action and mechanism of ketamine upon cardiovascular system, arterial blood pressure, tension of aortic ring, left ventricular developed pressure and heart rate were measured in rats, Ketamine produced two types of effects on arterial blood pressure in anesthetized rats; monophasic effect (blood pressure lowering) and biphasic effect (initial transient blood pressure increasing following sustained lowering), The ketamine-induced lowering of aterial blood pressure showed a concentration-dependent manner, inhibited by the pretreament of $MgCl_2$ and potentiated by the pretreatment of $CaCl_2$. The ketamine-induced lowering of aterial blood pressure was suppressed by the pretreatment of nifedipine, verapamil or lidocaine. In phenylephrine-precontracted endothelium intact (+E) aortic rings, ketamine sometimes caused a small enhancement of contraction ($112.5{\pm}3.6{\%}$). However, in many experiments, ketamine produced a concentration-dependent relaxation in +E aortic rings precontracted with either phenylephrine or KCl. Ketamine-induced relaxation was significantly greater in KCl-precontracted strips than phenylephrine-precontracted strips. In phenylephrine-precontracted +E aortic rings, the ketamine-induced vasorelaxation was not suppressed by endothelium removal or by the pretreatment of a nitric oxide synthase inhibitors, L-$N^G$-nitro-arginine and a guanylate cyclase inhibitors, methylene blue, suggesting that the ketamine-induced vasorelaxation is not dependent on the endothelial function. In addition, ketamine elicited an increase in left ventricular developed pressure in perfused hearts accompanied by decrease in heart rate. These results suggest that ketamine could evoke a hypotension due to vasorelaxation and decrease in heart rate in rats. The inhibitory effect of cardiovascular system might be associated with modulation of $Ca^{2+}$ homeostasis.

• Structural Engineering and Mechanics
• /
• v.76 no.3
• /
• pp.311-324
• /
• 2020

Until now, a comparative study on fully and partially-connected steel shear walls leading to enhancing strength and stiffness reduction of partially-connected steel plate shear wall structures has not been reported. In this paper a number of 4-story and 8-story steel plate shear walls, are considered with three different connection details of infill plate to surrounding frame. The specimens are modeled using nonlinear finite element method verified excellently with the experimental results and analyzed under monotonic loading. A comparison between initial stiffness and shear strength of models as well as percentage of shear force by model boundary frame and infill plate are performed. Moreover, a comparison between energy dissipation, ductility factor and distribution of Von-Mises stresses of models are presented. According to the results, the initial stiffness, shear resistance, energy dissipation and ductility of the models with beam-only connected infill plates (SSW-BO) is found to be about 53%, 12%, 15% and 48% on average smaller than those of models with fully-connected infill plates (SPSW), respectively. However, performance characteristics of semi-supported steel shear walls (SSSW) containing secondary columns by simultaneously decreasing boundary frame strength and increasing thickness of infill plates are comparable to those of SPSWs. Results show that by using secondary columns as well as increasing thickness of infill plates, the stress demands on boundary frame decreases substantially by as much as 35%. A significant increase in infill plate share on shear capacity by as much as 95% and 72% progress for the 4-story SSW-BO and 8-story SSSW8, respectively, as compared with non-strengthened counterparts. A similar trend is achieved by strengthening secondary columns of 4-story SSSW leading to an increase of 50% in shear force contribution of infill plate.

• Journal of Korean Society of Steel Construction
• /
• v.15 no.5 s.66
• /
• pp.579-589
• /
• 2003

Most beam-to-column connections are symmetrically reinforced because of the reverse action caused by earthquakes. However, in weak-earthquake regions like Korea, asymmetrically reinforced connections could be used. In particular, the connections between concrete-filled tube (CFT) column and H-shape beam could be applied using a simplified lower diaphragm. The tensile capacity or Combined Cross Diaphragm for upper reinforcing was tested using a simple tension test. Four types for lower reinforcing combined Cross, none, horizontal T-bar, and vertical plate were tested using the ANSI/AISC SSPEC 2002 loading program. Horizontal T-bar and stud bolts in vertical flat, bar transmit tensile stress from the beam's bottom flange to filled concrete. All test specimens satisfied 0.01 radian inelastic rotational requirement in ordinary moment frame of AISC seismic provision. According to the results of the parametric studies simplified lower diaphragms demonstrated outstanding strength, stiffness, and plastic deformation capacity which could lead to more sufficient seismic performance in the field.

• Journal of Yeungnam Medical Science
• /
• v.9 no.2
• /
• pp.382-395
• /
• 1992

GABA is an inhibitory neurotransmitter in central nervous system and produce sedative, antianxiety and muscle reaxing effects via $GABA_A$ receptor or $GABA_B$ receptor. Recently it is known that GABA is widely distributed throughout peripheral organs and may playa physiological role in certain organ. The vas deferens is innervated by species-difference. These study, therefore, was performed to investigate the mode and the mechanism of action of GABA on the norepiniphrine-, ATP- and electric stimulation-induced contraction of vas deferens of rat. Sprague-Dawley rats were sacrificed by cervical dislocation. The smooth muscle strips were isolated from the prostastic portion and were mounted in the isolated muscle bath. PSS in the bath was aerated with 95/5%-$O_2/CO_2$ at $33^{\circ}C$. Muscle tensions were measured by isometric tension transducer and were recorded by biological recording system. 1. GABA, muscimol, a $GAB_A$ agonist, and baclofen, a $GABA_B$ agonist inhibited the electric field stimulation(EFS, 0.2Hz, 1mSec, 80 V, monophasic square wave)-induced contraction with a rank order of potency of GABA greater than baclofen greater than muscimol. 2. The inhibitory effect of GABA was antagonized by delta aminovaleric acid(DAVA), a $GABA_B$ antagonist, but not by bicuculline, a $GABA_A$ mtagonist. 3. The inhibitory effect of baclofen was antagonized by DAVA, but the effect of muscimol was not antagonized by bicuculline. 4. Exogenous norepinephrine(NE) and ATP contracted muscle strip concentration dependently, but the effect of acetylcholine was negligible : and GABA did not affect the NE-and ATP-induced contractions. 5. GABA, baclofen and muscimol did not affect basal tone, and GABA did not affect the NE-and ATP-induced contractionsm 6. EFS-induced contraction was including 2 distinctable components. The first phasic component was inhibited by beta gamma-methylene ATP(mATP), a desensitizing agent of APT receptor and the second tonic component was reduced by pretreatment of reserpine(3 mg/Kg, IP). 7. GABA inhibited the EFS-induced contraction of reserpinized strips, but not the mATP-treated strips. These results suggest that in the prostatic portion of the rat vas deferens, adrenergic and purinergic neurotransmissions are exist, and GABA inhibits the release of ATP via presynaptic $GABA_B$ receptor on the excitatory neurons.