• Title/Summary/Keyword: Temperature response function

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Development and Validation of a Predictive Model for Listeria monocytogenes Scott A as a Function of Temperature, pH, and Commercial Mixture of Potassium Lactate and Sodium Diacetate

  • Abou-Zeid, Khaled A.;Oscar, Thomas P.;Schwarz, Jurgen G.;Hashem, Fawzy M.;Whiting, Richard C.;Yoon, Kisun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.7
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    • pp.718-726
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    • 2009
  • The objective of this study was to develop and validate secondary models that can predict growth parameters of L. monocytogenes Scott A as a function of concentrations (0-3%) of a commercial potassium lactate (PL) and sodium diacetate (SDA) mixture, pH (5.5-7.0), and temperature (4-37DC). A total of 120 growth curves were fitted to the Baranyi primary model that directly estimates lag time (LT) and specific growth rate (SGR). The effects of the variables on L. monocytogenes Scott A growth kinetics were modeled by response surface analysis using quadratic and cubic polynomial models of the natural logarithm transformation of both LT and SGR. Model performance was evaluated with dependent data and independent data using the prediction bias ($B_f$) and accuracy factors ($A_f$) as well as the acceptable prediction zone method [percentage of relative errors (%RE)]. Comparison of predicted versus observed values of SGR indicated that the cubic model fits better than the quadratic model, particularly at 4 and $10^{\circ}C$. The $B_f$and $A_f$for independent SGR were 1.00 and 1.08 for the cubic model and 1.08 and 1.16 for the quadratic model, respectively. For cubic and quadratic models, the %REs for the independent SGR data were 92.6 and 85.7, respectively. Both quadratic and cubic polynomial models for SGR and LT provided acceptable predictions of L. monocytogenes Scott A growth in the matrix of conditions described in the present study. Model performance can be more accurately evaluated with $B_f$and $A_f$and % RE together.

Prediction of Setting Time of Concrete Using Fly Ash and Super Retarding Agent (초지연제 및 플라이애쉬를 사용한 콘크리트의 응결시간 예측)

  • Han, Min-Cheol
    • Journal of the Korea Concrete Institute
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    • v.18 no.6 s.96
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    • pp.759-767
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    • 2006
  • This paper presents a method to estimate the setting time of concrete using super retarding agent(SRA) and fly ash(FA) under various curing temperature conditions by applying maturity based on equivalent age. To estimate setting time, the equivalent age using apparent activation energy($E_a$) was applied. Increasing SRA content and decreasing curing temperature leads to retard initial and final set markedly. $E_a$ at the initial set and final set obtained by Arrhenius function showed differences in response to mixture type. It is estimated to be from $24{\sim}35KJ/mol$ in all mixtures, which is smaller than that of conventional mixture ranging from $30{\sim}50KJ/mol$. Based on the application of $E_a$ to Freisleben-Hansen and Pederson's equivalent age function, equivalent age is nearly constant, regardless of curing temperature and SRA contents. This implies that the concept of maturity is applicable in estimating the setting time of concrete containing SRA. A high correlation between estimated setting time and measured setting time is observed. Multi-regression model to determine appropriate dosage of SRA reflecting FA contents and equivalent age was provided. Thus, the setting time estimation method studied herein can be applicable to the concrete containing SRA and FA in construction fields.

The Study on the Role of Prostaglandin in Contraction of Vas Deferens (정관운동에 있어서 prostaglandin 의 역할에 관한 연구)

  • Park, Won-Kyoo
    • The Korean Journal of Pharmacology
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    • v.19 no.2
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    • pp.1-8
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    • 1983
  • Prostaglandin(PG) is ubiquitously distributed in most mammalian tissue and their actions are complicated. Especially in autonomic nervous system, there are evidences indicating that PGs act as neuromodulators i.e., PGs, which are released in the vicinity of autonomic neuroeffector junctions, influence the release and the response of the neurotransmitter. Present study was undertaken to elucidate the interrelationship between $PGF_{2\alpha}$ and adrenergic ${\alpha}_2-receptor$ function in electrical field stimulation induced contractile response of vas deferens in rat. Male rat, weighing 150{\sim}200\;g, was sacrificed and vas deferens was obtained. The isolated vas deferens strip was placed between two platinum electrodes in temperature controlled $(37^{\circ}C)$ muscle chamber containing Tyrode's solution and the electrical field stimulation(EFS) induced contraction was recorded with Grass Polygraph(Model 7) via force displacement transducer (FT .03, Grass). The results are summarized as follows: 1) Electrical field stimulation for 1sec( 1 msec, 40 cps) induced contraction of vas deferens was completely blocked by tetrodotoxin. 2) Bretylium caused marked inhibition of the EFS-induced contraction, hut tyramine and cocaine augmented the contraction. 3) EFS-induced contraction was inhibited or little affected in distal portion of vas deferens by norepinephrine or methoxamine, but the contraction was rather augmented by the ${\alpha}-agonists$ in proximal portion. 4) Clonidine inhibited the EFS-induced contraction proportionally to the concentration in distal portion, which was blocked by yohimbine pretreatment, but in the presence of $PGF_{2\alpha}$ the blockade by yohimbine was reversed. 5) Indomethacin pretreatment reduced the effect of clonidine, but addition of $PGF_{2\alpha}$ after washing-out the indomethacin caused the contraction to the control level. From these results it is suggested that PG synthesis is a necessary step and the PG itself has a permissive role in ${\alpha}_2-adrenoceptor$ action in rat vas deferens.

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The Effect of Different Type of Exercise on SOD, Neutrophils and T Lymphocytes (훈련방법의 차이가 SOD, Neutrophils 및 T세포에 미치는 영향)

  • Kwak, Yi-Sub;Um, Sang-Yong;Kim, Dong-Eun;Hwang, Hye-Jin
    • IMMUNE NETWORK
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    • v.5 no.4
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    • pp.232-236
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    • 2005
  • Background: A physically active lifestyle and regular exercise training incurs many health benefits. One recently recognized benefit of regular moderate exercise is stress reduction and immune enhancement. Thus, a physical stress such as exercise may act at any number of points in the complex sequence of events collectively termed the immune response. Although exercise causes many propound changes in parameters of immune function, the nature and magnitude of such changes rely on several factors including the immune parameters of interest; type, intensity, and duration of exercise; fitness level or exercise history of the subject; environmental factors such as ambient temperature and humidity. Methods: This study was undertaken to investigate the effect of different type of exercise on superoxide dismutase (SOD), neutrophils, and T lymphocytes of Sprague-Dawley rats. Sprague-Dawley rats were randomly divided into three groups; a non-Trained group (NTG, n=6), a swim-Trained group (STG), and a treadmill-Trained group (TTG). The exercise regimen was designed in a treadmill (5 times/5 days/week) during 8-weeks for TTG, and swim training (5 times/5 days/week) during 8-weeks for STG, and the volume of exercise training was the same in both groups. Results: 8 weeks of regular swim and treadmill training significantly increased liver SOD concentration however, muscle SOD concentration was not statistically significant. In the level of neutrophils, TTG and STG showed significant difference, compared to NTG. TTG was the highest level of neutrophils. In the level of immune cell counts, there was significant difference among TTG, STG, and NTG both in the spleen and thymus. Conculsion: In conclusion, it can be stated that eight weeks swim and treadmill exercise training has beneficial effect in improving immune response and antioxidant defence capacity by augmenting immune cells and SOD activities of SD rats.

A Study of the Adaptive Control System (適應制御裝置에 關한 硏究)

  • Ha, Joo-Shik;Choi, Kyung-Sam;Kim, Seung-Ho
    • Journal of Advanced Marine Engineering and Technology
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    • v.3 no.1
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    • pp.19-31
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    • 1979
  • Recently the adaptive control system, which keeps the control system always optimal by adjusting the control parameters automatically according to the variations of the plant parameters, have become very important in the field of control engineering. The adaptive control systems are usally composed of the plant identification, the decision of the optimal control parameters, and the adjustment of the control parameters. This paper deals with a method of the adaptive control system when PI or PID controller is used in the feed back control system. Its controlled object (the plant) is assumed to be described by the transfer function of $\frac{ke^{-LS}}{1+TS}$ where k, T and L are steady state gain, time constant and pure dead time respectively, and their values are variable in accordance with the change of environmental circumstance. It has been known that a pseudo-random binary signal is quite effective for the measurement of an impulse response of a plant. In adaptive control systems, however, the impulse response itself is not appropriate to determine the control parameters. In this paper, the authors propose a method to estimate directly the parameters of the plant k, T and L by means of the correlation technique using 3 level M-sequence signal as a test signal. The authors also propose a method to determine the optimal parameters of the PI or PID controller in the sense of minimizing the square integral of the control error in the feed back control system, and the values of the optimal parameters are computed numerically for various values of T and L, and the results are examined and compared with those of the conventional methods. Finally the above-mentioned two methods are combined and an algorithm to struct an adaptive control system is suggested. The experiments for the indicial responses by means of both the model of the temperature control system using SCR actuater and the analog simulations have shown good results as expected, and the effectiveness of the proposed method is verified. The M-sequence generator and the time delay circuit, which are manufactured for the experiments, are operated in quite a good condition.

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An Online Calibration Algorithm for Cellular CDMA Antenna Arrays (Cellular CDMA용 배열 안테나 오차 보정 알고리듬)

  • 석미경;조상우;전주환
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.2C
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    • pp.306-314
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    • 2004
  • Some receiver(and most transmit) beamforming algorithms with an array antenna at a cellular CDMA base stations require accurate internal and external calibrations. The external calibration, which usually needs to be done only once, determines the array manifolds, i.e. the complex response of each antenna as a function of DOA(Directions of Arrival). The internal calibrations are necessary because characteristics of RF/IF circuity of each receiver chain vary differently in response to temperature or humidity changes. We propose an iterative subspace-based calibration algorithm for an asynchronous CDMA-based antenna away in the presence of unknown gain and phase error is presented. We verify the subspace-based calibration algorithms by performing the experiment using measured data. Also, we propose an efficient algorithm using the simulated annealing technique. This algorithm overcomes the problem of the initial guessing in the subspace-based approach.

Isolation and Characterization of a Type II Peroxiredoxin Gene from Panax ginseng C. A. Meyer

  • Kim, Yu-Jin;Lee, Jung-Hye;Lee, Ok-Ran;Shim, Ju-Sun;Jung, Seok-Kyu;Son, Na-Ri;Kim, Ju-Han;Kim, Se-Young;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.34 no.4
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    • pp.296-303
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    • 2010
  • A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

Enhanced extraction of copper and nickel based on the Egyptian Abu Swayeil copper ore

  • Somia T. Mohamed;Abeer A. Emam;Wael M. Fathy;Amany R. Salem;Amr B. ElDeeb
    • Analytical Science and Technology
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    • v.37 no.1
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    • pp.63-78
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    • 2024
  • The continuous increasing of the global demand of copper and nickel metals raises the interest in developing alternative technologies to produce them from copper sulfide ore. Also, in line with Egypt's vision 2030 for achieving the sustainable socioeconomic development which aims at developing alternative and eco-friendly technologies for processing the Egyptian ores to produce these strategic products instead of its importing. These metals enhance the advanced electrical and electronic industries. The current work aims at investigating the recovery of copper and nickel from Abu Swayeil copper ore using pug leaching technique by sulfuric acid. The factors affecting the pug leaching process including the sulfuric acid concentration, leaching time and temperature have been investigated. The copper ore sample was characterized chemically using X-ray fluorescence (XRF) and scanning electron microscope (SEM-EDX). A response surface methodology develops a quadratic model that expects the nickel and copper leaching effectiveness as a function of three controlling factors involved in the procedure of leaching was also investigated. The obtained results showed that the maximum dissolution efficiency of Ni and Cu are 99.06 % and 95.30%, respectively which was obtained at the following conditions: 15 % H2SO4 acid concentration for 6 hr. at 250 ℃. The dissolution kinetics of nickel and copper that were examined according to heterogeneous model, indicated that the dissolution rates were controlled by surface chemical process during the pug leaching. The activation energy of copper and nickel dissolution were 26.79 kJ.mol-1 and 38.078 kJ.mol-1 respectively; and the surface chemical was proposed as the leaching rate-controlling step.

Isolation of cDNA Encoding Low Temperature-inducible L-asparaginase from Soybean (Glycin max) (저온 스트레스에 발현이 유도되는 콩의 L-asparaginase 유전자의 분리)

  • Park, Seong-Whan;Kim, Kee-Young;Chen, Liang;Lee, Jai-Heon
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.99-104
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    • 2002
  • Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.

Fabrication of barium titanate-bismuth ferrite fibers using electrospinning

  • Baji, Avinash;Abtahi, Mojtaba
    • Advances in nano research
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    • v.1 no.4
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    • pp.183-192
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    • 2013
  • One-dimensional multiferroic nanostructured composites have drawn increasing interest as they show tremendous potential for multifunctional devices and applications. Herein, we report the synthesis, structural and dielectric characterization of barium titanate ($BaTiO_3$)-bismuth ferrite ($BiFeO_3$) composite fibers that were obtained using a novel sol-gel based electrospinning technique. The microstructure of the fibers was investigated using scanning electron microscopy and transmission electron microscopy. The fibers had an average diameter of 120 nm and were composed of nanoparticles. X-ray diffraction (XRD) study of the composite fibers demonstrated that the fibers are composed of perovskite cubic $BaTiO_3$-$BiFeO_3$ crystallites. The magnetic hysteresis loops of the resultant fibers demonstrated that the fibers were ferromagnetic with magnetic coercivity of 1500 Oe and saturation magnetization of 1.55 emu/g at room temperature (300 K). Additionally, the dielectric response of the composite fibers was characterized as a function of frequency. Their dielectric permittivity was found to be 140 and their dielectric loss was low in the frequency range from 1000 Hz to $10^7$ Hz.