• 제목/요약/키워드: Tea tree oil

검색결과 61건 처리시간 0.027초

In Vitro and In Vivo Anti-Tobacco Mosaic Virus Activities of Essential Oils and Individual Compounds

  • Lu, Min;Han, Zhiqiang;Xu, Yun;Yao, Lei
    • Journal of Microbiology and Biotechnology
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    • 제23권6호
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    • pp.771-778
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    • 2013
  • Essential oils are increasingly of interest for use as novel drugs acting as antimicrobial and antiviral agents. In the present study, we report the in vitro antiviral activities of 29 essential oils, extracted from Chinese indigenous aromatic plants, against the tobacco mosaic virus (TMV). Of these essential oils, those oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass effected a more than 50% inhibition of TMV at 100 ${\mu}g/ml$. In addition, the mode of antiviral action of the active essential oils was also determined. Essential oils isolated from artemisia and lemongrass possessed potent inactivation and curative effects in vivo and had a directly passivating effect on TMV infection in a dose-dependent manner. However, all other active essential oils exhibited a moderate protective effect in vivo. The chemical constitutions of the essential oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass were identified by gas chromatography and gas chromatography-mass spectrometry. The major components of these essential oils were ${\alpha}$-zingiberene (35.21%), limonene (76.25%), terpinen-4-ol (41.20%), limonene (80.95%), 1,8-cineole (27.45%), and terpinolene (10.67%). The curative effects of 10 individual compounds from the active essential oils on TMV infection were also examined in vivo. The compounds from citronellal, limonene, 1,8-cineole, and ${\alpha}$-zingiberene effected a more than 40% inhibition rate for TMV infection, and the other compounds demonstrated moderate activities at 320 ${\mu}g/ml$ in vivo. There results indicate that the essential oils isolated from artemisia and lemongrass, and the individual compound citronellal, have the potential to be used as an effective alternative for the treatment of tobacco plants infected with TMV under greenhouse conditions.

개에서 분리된 $Malassezia$ $pachydermatis$의 특성과 Essential Oil의 항진균 효과 (Characteristics of $Malassezia$ $pachydermatis$ Isolated from Dogs and Antifungal Effect of Essential Oils)

  • 김주연;;손원근
    • 한국임상수의학회지
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    • 제29권2호
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    • pp.141-147
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    • 2012
  • 이 연구는 개의 귀 속에서 분리한 $Malassezia$ $pachydermatis$의 특성과 이들 효모에 대한 essential oil들의 항진균효과를 측정한 결과로서 일반적인 $M.$ $pachydermatis$를 분리하기위한 Sabouraud dextrose agar (SDA), olive oil이 첨가된 SDA (SDAO) 배지 및 지방 친화성 효모를 검출하기 위한 Leeming's medium (LM)으로 구성된 세종류의 분리배지가 사용되었다. $Malassezia$ spp는 77.8%의 개로부터 분리되었으며 분리배지에 따라 분리율에 큰 차이를 나타내었으며 지방성분이 포함된 배지에서의 분리율이 일반적으로 사용하는 SDA에서 보다 높았다. 분리된 모든 $Malassezia$ spp는 $M.$ $pachydermatis$로 동정되었지만 육안적으로 보이는 집락의 모양과 SDA에서의 증식속도를 고려했을 때 4개의 아종으로 관찰되었다. 배양 72시간 후 집락의 직경이 3 mm 이상인 큰 집락(LC)과 2 mm 정도인 중간 집락(MC) 및 배양 5일 후에도 1 mm 이상으로 크지지 않는 작은 집락(SC), 그리고 SDA에서 증식하지 않는 지방의존 집락(Lipo)으로 분류되었다. SDA, SDAO 및 LM 배지에서 각 type의 효모가 분리된 정도를 보면, LC type 효모는 각각 4, 11, 11개 시료에서, MC type 효모는 각각 2, 3, 1개 시료에서, SC type 효모는 각각 1, 2, 1개시료에서 분리되었으며 Lipo type은 SDAO과 LM 배지 중 3 곳에서 분리되었다. $M.$ $pachydermatis$에 대한 essential oil의 항진균효과 측정은 디스크 확산법과 well 확산법에 의해 이루어졌으며, 대부분의 essential oil은 0.5에서 1.0%의 농도에서 $M.$ $pachydermatis$ 의 증식을 억제하였다. MIC90과 MIC50은 essential oil의 성분에 따라 다양하였으나, Thyme oil이 가장 억제 효과가 좋았으며 marjoram과 tea tree oil은 비교적 낮은 억제 능력을 나타내었다.

Activity of Essential Oils Against Bacillus subtilis Spores

  • Lawrence, Hayley A.;Palombo, Enzo A.
    • Journal of Microbiology and Biotechnology
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    • 제19권12호
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    • pp.1590-1595
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    • 2009
  • Alternative methods for controlling bacterial endospore contamination are desired in a range of industries and applications. Attention has recently turned to natural products, such as essential oils, which have sporicidal activity. In this study, a selection of essential oils was investigated to identify those with activity against Bacillus subtilis spores. Spores were exposed to 13 essential oils, and surviving spores were enumerated. Cardamom, tea tree, and juniper leaf oils were the most effective, reducing the number of viable spores by 3 logs at concentrations above 1%. Sporicidal activity was enhanced at high temperatures ($60^{\circ}C$) or longer exposure times (up to 1 week). Gas chromatography-mass spectrometry analysis identified the components of the active essential oils. However, none of the major oil components exhibited equivalent activity to the whole oils. The fact that oil components, either alone or in combination, did not show the same level of sporicidal activity as the complete oils suggested that minor components may be involved, or that these act synergistically with major components. Scanning electron microscopy was used to examine spores after exposure to essential oils and suggested that leakage of spore contents was the likely mode of sporicidal action. Our data have shown that essential oils exert sporicidal activity and may be useful in applications where bacterial spore reduction is desired.

잔토리졸의 칸디다균 항균 효과 (Microbiological Effects of Xanthorrhizol against Candida albicans)

  • 조완구;김효진
    • 한국응용과학기술학회지
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    • 제27권1호
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    • pp.37-43
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    • 2010
  • The objective of this study was to evaluate the antifungal properties of xanthorrhizol (extract from curcuma xanthorrhiza) against Candida albicans. Some of the commercial products were evaluated for their ability to inhibit growth of C. albicans. OTC product containing povidone iodide and cosmetic grade product with surfactants were tested. Antifungal activity was shown in 1.56% of OTC product, however, there was no antifungal effect in cosmetic product. For the comparison, we tested several materials. Povidone iodide, lemon tea tree oil and xanthorrhizol showed antifungal activities against C albicans in 0.25, 0.062 and 0.007%, respectively. We also tested the antifungal effects of povidone iodide and xanthorrhizol in surfactant base. Test results revealed that 2.5% of povidone iodide and 0.156% of xanthorrhizol showed similar antifungal effects. These findings support the application of xanthorrhizol for vaginal cleanser or personal cares using antifungal effect.

다양한 화상 응급처치의 치료효과 비교 (Comparison of Clinical Outcomes Using Various First-Aid Cooling Method for Burn)

  • 이정환;송우진;권민주;박양서;고장휴
    • 대한화상학회지
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    • 제24권1호
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    • pp.7-13
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    • 2021
  • Purpose: Currently, the most recommended method for burn first aid is the cool running water for 20 minutes (CRW20). If CRW20 is not practicable, cooling dressing products with tea tree oil are used as substitutes. In this study, we analyze the effect of various burn first aids, including CRW20, cooling products, on the treatment period. Methods: This study was conducted on patients who suffered burns and visited Hangangsoo Hospital from March 2019 to March 2020. The study conducted in a retrospective method. The duration of treatment was analyzed by dividing the experimental group. Results: The treatment period was shortened when first aid was given (P<0.001). Cold water, ice, and ice packs resulted in reduced treatment periods (P<0.001, P=0.004). The treatment period was reduced when cooling dressing products were used in all groups (P=0.041). The implementation of first aid has reduced the duration of burn treatment. This means that cooling helps burn treatment. CRW20 did not show statistically meaningful results. This is the result of a failure to fully control the temperature and time of tap water. In addition, the change in temperature of tap water according to season, the use of water is higher than that of animal testing, and the general lack of information on proper burn first aid may have affected. Conclusion: The treatment period was statistically significantly reduced in the group that performed first aid. Among the various first aid methods, cold water and ice reduced the treatment period.

블렌딩 에센션오일의 항균효과 증진 (Improved Antibacterial Effect of Blending Essential Oils)

  • 권필승;김대중;박호
    • 대한임상검사과학회지
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    • 제49권3호
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    • pp.256-262
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    • 2017
  • 식물로부터 유래되는 휘발성 유기 화합물인 에센셜 오일은 인체에 약리효과를 나타낸다고 알려져 있다. 본 연구에서는 화농성 질환과 병독성 질환 비뇨기계통 감염에 나올 수 있는 병원성 세균 Staphylococcus aureus (그람양성알균), Escherichia coli (그람음성막대균), Pseudomonas aeruginosa (그람음성막대균, 극성단모균)을 대상으로 천연 허브 오일 중에 비교적 많이 알려져 있는 라벤더(lavender), 티트리(tea tree), 로즈마리(rosemary), 멜리사(melisa) 4종류의 단일 오일과 비교해, 티트리와 로즈마리, 멜리사를 블렌딩하였을 때 항균력이 상승되는 효과가 있는지 연구하였다. 그 결과 디스크 확산법에서 블렌딩 오일(티트리, 로즈마리, 멜리사)의 경우 단독의 허브오일보다 S. aureus에서18 mm, E. coli 에서 22 mm 와 P. aeruginosa는13 mm의 우수한 항균능력을 확인할 수 있었다. 뿐만 아니라 블렌딩 오일의 경우 최소억제농도(Minimum Inhibitory Concentrations, MIC) 분석에서 S. aureus, E. coli 에서는 0.3125%에서, P. aeruginosa는 1.25%에서 성장억제효과가 확인되었다. 따라서 본 실험에서는 블렌딩 오일이 Oxacillin과 로즈마리, 티트리보다 항균효과가 우수한 것으로 증명되어 단독의 허브오일보다 블렌딩 오일이 우수한 항균력을 나타나는 것을 확인할 수 있었다. 그러므로 허브의 블렌딩을 활용하면 다제 내성균에 대한 항균시너지효과를 발휘할 수 있음을 입증했을 뿐만 아니라 향후 천연보존제와 건강기능성 식품 및 화장품에 활용 가능성을 기대해본다.

Investigation of bacteremia after toothbrushing in orthodontic patients

  • Hussein, Emad Ahmad;Acar, Ahu;Dogan, Alev Aksoy;Kadir, Tanju;Caldemir, Seniz;Erverdi, Nejat
    • 대한치과교정학회지
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    • 제39권3호
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    • pp.177-184
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    • 2009
  • Objective: The aim of this study was to investigate the occurrence rate of bacteremia following toothbrushing with toothpastes composed of several antibacterial agents and compare the results with the conventional oral hygiene maintaining methods in orthodontic patients. Methods: This clinical study included 100 adult orthodontic patients who were divided into 4 groups. Each group comprised of 25 patients, wearing fixed orthodontic appliances. In the first group, bacteremia was assessed after toothbrushing without using any toothpaste. In the second group, a 0.2% chlorhexidine gluconate mouthrinse was used before brushing with no toothpaste. In the third group, subjects brushed with a commonly used toothpaste which did not include an additional antimicrobial agent. The fourth experimental group used toothpaste which included tea tree oil, clove oil, peppermint oil and bisabolol as antimicrobial elements. Pre- and post-brushing blood samples were obtained using a strict aseptic technique. All samples were microbiologically evaluated using blood culture bottles. Results: Toothbrushing in orthodontic patients yielded to an increase in the occurrence rate of bacteremia when using normal toothpaste or no toothpaste at all. Conclusions: The use of chlorhexidine mouthwash before toothbrushing, and brushing with antimicrobial toothpaste did not show a statistically significant difference in preventing the occurrence of bacteremia (p > 0.05).

The mechanical properties of 3D printed denture base resin incorporating essential oil microcapsules

  • Steve An;Jane Lesleigh Evans;Stephen Hamlet;Robert Matthew Love
    • The Journal of Advanced Prosthodontics
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    • 제15권4호
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    • pp.189-201
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    • 2023
  • PURPOSE. The aim of this study was to investigate the mechanical properties of three-dimensional (3D) printed denture base resin incorporating microcapsules containing plant essential oils. MATERIALS AND METHODS. Denture base specimens containing up to 3% w/v essential oil microcapsule powders (MCPs), i.e., eucalyptus, geranium, lavender, menthol, and tea tree, in two resins (Detax and NextDent 3D+) were 3D printed using two printers (Asiga and NextDent 5100). The dispersion and interaction of the MCPs in the resin were assessed by SEM while the mechanical properties of the incorporated denture base including flexural strength (MPa), flexural modulus (MPa), Vickers hardness (VHN), and surface roughness (Ra) were also subsequently evaluated. Statistical analysis of any differences in mean values was determined using a two-way ANOVA with Tukey's post hoc testing (α = .05). RESULTS. The spherical shape of the MCPs was maintained during the mixing and polymerization/printing process. However, the Detax-Asiga group showed significant agglomeration of the MCPs even at the lowest MCP concentration levels (0.5% w/v). Overall, as the microcapsule concentration increased, the mean flexural strength decreased, though the menthol MCP groups remained compliant with the ISO standard. The flexural modulus and harness remained relatively unchanged, and the flexural modulus complied with the ISO standard regardless of the MCP concentration. Surface roughness increased with the addition of the MCPs but also remained below that required for clinical acceptance. CONCLUSION. Incorporation of microencapsulated plant essential oils into 3D printed denture base resin was successfully achieved. While incorporation negatively influenced flexural strength and surface roughness, little effect on flexural modulus and Vickers hardness was demonstrated.

Antifungal Activities of the Essential Oils in Syzygium aromaticum (L.) Merr. Et Perry and Leptospermum petersonii Bailey and their Constituents against Various Dermatophytes

  • Park, Mi-Jin;Gwak, Ki-Seob;Yang, In;Choi, Won-Sil;Jo, Hyun-Jin;Chang, Je-Won;Jeung, Eui-Bae;Choi, In-Gyu
    • Journal of Microbiology
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    • 제45권5호
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    • pp.460-465
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    • 2007
  • This study was carried out in order to investigate the potential of using plant oils derived from Leptospermum petersonii Bailey and Syzygium aromaticum L. Merr. Et Perry as natural antifungal agents. The antifungal effects of essential oils at concentrations of 0.05, 0.1, 0.15, and 0.2 mg/ml on the dermatophytes Microsporum canis (KCTC 6591), Trichophyton mentagrophytes (KCTC 6077), Trichophyton rubrum (KCCM 60443), Epidermophyton floccosum (KCCM 11667), and Microsporum gypseum were evaluated using the agar diffusion method. The major constituents of the active fraction against the dermatophytes were identified by gas chromatography-mass spectrometry and high-performance liquid chromatography analysis. The antifungal activities of S. aromaticum oil (clove oil) against the dermatophytes tested were highest at a concentration of 0.2mg/ml, with an effectiveness of more than 60%. Hyphal growth was completely inhibited in T. mentagrophytes, T. rubrum, and M. gypseum by treatment with clove oil at a concentration of 0.2 mg/ml. Eugenol was the most effective antifungal constituent of clove oil against the dermatophytes T. mentagrophytes and M. canis. Morphological changes in the hyphae of T. mentagrophytes, such as damage to the cell wall and cell membrane and the expansion of the endoplasmic reticulum, after treatment with 0.11 mg/ml eugenol were observed by transmission electron microscopy (TEM). At a concentration of 0.2 mg/ml, L. petersonii oil (LPO) was more than 90% effective against all of the dermatophytes tested, with the exception of T. rubrum. Geranial was determined to be the most active antifungal constituent of L. petersonii oil. Taken together, the results of this study demonstrate that clove and tea tree oils exhibited significant antifungal activities against the dermatophytes tested in this study.

한국의 전통적 기능성 식품의 이용에 대한 역사적 고찰 (The Historical Study of Korean Traditional Funtional Food)

  • 한복진
    • 동아시아식생활학회지
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    • 제6권2호
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    • pp.235-255
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    • 1996
  • Natural substances, exploited in our daily life, have been applied to drugs to treat diseases and developed to functional foods by appropriate preparations, and these foods give beneficial effects on physical activities. In this paper, the utilization of traditional functional foods was studied with refer ring to old ancient writings published in the front-end of Chosun dynasty. The utilized vegetables were march mallow, turnip, radish, Chinese cabbage, lettuce, spinach, cucumber winter buds, flesh of a cabbage, eggplant, taro, burdock, Parsley, watershield plant, crown daisy, bamboo shoots, garlic, scallion, onion, acorn, bark of a tree, white goosefoot leaf, leaf of bean, pine mushroom, bracken. yam, mugwort, tea, ginseng, peppermint, fruit of the Maximowiczia chinensis, smartweed and pepper. The utilized fruits were chestnut, Chinese date, pine nuts, walnut, gingko nut, citrus. crab apple, pear, peach, grape, pomegranate, plum, Chinese quince, fig and watermelon. The utilized cereal were rice, barley, bean, buckweat and Job's-tears. The utilized sweetenings and seasonings were honey, wheat-gluten, sugar, oil, salt, soy sauce and vinegar. Our ancestors had a balanced diet using the various foods, and especially had a fundamental concept of "Foods have the efficacy of a remedy".edy".uot;.

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