• Journal of Plant Biology
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• v.36 no.1
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• pp.91-96
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• 1993

Phycoerythrins from the ceramiaceous red algae Campylaephora crassa (Okamura) Nakamura and related species, C. hypnaeoides J. Agardh and Ceramium kondoi Yendo, were investigated for absorption spectra, protein bands by gel electrophoresis and antigenic reactivity to anti-phycoerythrin using Ouchterlony double diffusion and immunoblot. Similarities in absorption spectra, showing peaks at ca. 566 nm>534 nm>495 nm, were found between C. crassa and Cm. kondoi, while C. hypnaeoides differed slightly. There were no differences in fluorescence emission spectra and protein bands between C. crassa and related species tested. Since Ouchterlony double diffusion, however, showed that phycoerythrins from C. crassa and Cm. kondoi were similar in antigenic reactions, and differed from that of C. hypnaeoides, the taxonomic position of C. crassa should be reinvestigated using other experimental approaches.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.159-163
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• 2000

The gudgeon, Microphysogobio tungtingensis uchidai, originally described by Banarescu and Nalbant in 1973 is reduced to a junior synonym of M. yaluensis based on the 5 type specimens in the USNM. Confusion on the taxonomic position of Microphysogovbio t. uchidai seems to have resulted from the inadequate description of the papillae of the upper lip and the reference to the small specimens.

• Journal of Microbiology
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• v.35 no.3
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• pp.222-227
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• 1997

DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHSl. Comparison of the deduced amino acid sequence of BbCHSl with those of other Euascomycetes revealed that BbCHSl is a gene for class II chitin synthase. The Blastp search of the deduced amino acid sequence of BbCHSl displayed the highest rate of similarity, 95.8%, with CHS2 of Metarhizium unisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongniartii, which was previously derived by traditional fungal classification based on morphological features.

• ALGAE
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• v.36 no.2
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• pp.123-135
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• 2021

The taxonomy of green microalgae relies traditionally on morphological traits but has been rapidly changing since the advent of molecular methods. Stichococcus Nägeli is a cosmopolitan terrestrial algal genus of the class Trebouxiophyceae that has recently been split into seven lineages, which, along with Pseudostichococcus, comprise the Stichococcuslike group; there is a need to further characterize these genera, since they are morphologically enigmatic. Here we used organic osmolytes as chemotaxonomic marker to verify the phylogenetic position of Stichococcus-like strains and were also able to exclude a strain hitherto identified as Gloeotila contorta from this group. Stichococcus-like organisms, including those recently revised, were characterized by the production of the polyol sorbitol and the disaccharide sucrose in high amounts, as is typical of Prasiola-clade algae. The results demonstrate that organic osmolyte chemotaxonomy can support green algal taxonomic designations as fundamental research.

• Mycobiology
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• v.49 no.1
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• pp.46-53
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• 2021

In a study of the fungal diversity on Ulleung Island in Korea, three novel strains of Penicillium were isolated. Different sites on Ulleung Island were selected for collecting endophytic fungi, and three endophytic fungal strains showed unique morphological characteristics. DNA sequence of the internal transcribed spacer, β-tubulin, calmodulin, and RNA polymerase II second largest subunit regions of the strains were analyzed and they showed unique taxonomic position from the other species of Penicillium section Sclerotiora. The new strains were named Penicillium ulleungdoense sp. nov. As the novel endophytic Penicillium taxa were discovered in a unique environment, the data could be meaningful for understanding the geographical distribution of Ascomycetes on Ulleung Island.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.37-53
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• 2001

Two regions of mtDNA genome, cytochrome oxidase subunit I (COI) and 165 ribosomal RNA (165 rRNA) genes, were sequenced for 15 species of the long-horned beetle belonging to four subfamilies and geographic samples of mulberry longicorn beetle, Apriona germari, from two localities in Korea. Ten samples of A. germari collected from Suwon and Busan revealed three COI haplotypes ranging in nucleotide divergence of 0.3% to 0.5%, and the two populations shared one common COI haplotype (80%). The sequence divergence among 15 species of the long-horned beetle was much higher in COI gene (12.3%∼39.4%) than 16S rRNA gene (7.2% to 23.1), and the maximum value in the COI gene is exceptional compared with other relevant studies, including that of Coleoptera. The greatly increased divergence in the COI gene, in facto was stemmed from a peculiar sequence of Prionus insularis belonging to Prioninne, divergence of which ranges from 31.2% to 39.3% from other species. We discussed possible reason of the divergence in this species. Due to the abnormality of COI gene divergence, decrease in phylogenetic signal was severe in COI nucleotide and, subsequently, the converted amino acid sequences, rendering us to put more confidence on the 16S5 rRNA gene data. Although the molecular phylogeny confidently supports the monophyletic origin of Lepturinae, the presence of discrepancy between molecular data and traditional taxonomic views also is a testable hyothesis. One such discrepancy includes taxonomic position of Sophronica obrioides and Theophilea cylindricollis belonging to Lamiinae.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.66-79
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• 2000

Anatomical characters such as stem, leaf, ovary, calyx lobe, ultrastructure of stigma, epidermis of leaf blade and midvein, corolla lobe, nectary, seed coat and pollen were examined on 6 taxa of Korean Ophelia, including 5 taxa distributed in south Korea and one taxon considered to be the variation type of Ophelia wilfordi, in order to clarify the limits of intersection and interspecies and to establish the taxonomic position. One taxon distributed in north Korea was also included in the description of species by observation of herbarium specimen of the University of Tokyo in Japan. The two sections were successfully distinguished by internal structure of ovary, morphology of nectary, surface sculpturing of corolla lobe and pollen, ultrastructure of seed and seed coat, which were useful characters to distinguish taxa higher than species. The variation type of Ophelia wilfordi was not distinguished with other species except for absent or present of purple spot in corolla lobe.

• Journal of Plant Biology
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• v.34 no.1
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• pp.59-66
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• 1991

The morphotaxonomic characters and life history of Symphyocladia latiuscula (Harvey) Yamada were investigated with field and laboratory culture materials. The species is well defined by a 5-15 cm hight, entirely corticated thallus and congenital fusion of 5-7 segments between main axis and laterals. Although all the reproductive structures are basically similar to those of other species of the genus, it is characteristic that the pinnulae are transformed into the flabellated tetrasporangial stichidia. Vegetative trichoblasts are known to be absent in the Pterosiphonieae, but their presence in S latiuscula does not accept its taxonomic position in that tribe. As a result, the occurrence of vegetative trichoblast is not useful for a taxonomic character to distinguish the tribes. The species shows a Polysiphonia-type life history involving a dimorphic alternation of gametophytes and sporophytes in culture and field. It grows below the middle intertidal zone in all the coasts of Korea throughout the year, although fertile plants are normally found during the summer months.months.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.257-270
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• 2012

The taxonomic relationship of 3 taxa in $Carex$ sect. $Siderostictae$ were revealed by morphological and molecular studies. The morphological characteristics of Korean $Carex$ siderosticta Hance, which is a type species of sect. $Siderostictae$, was first reviewed in comparison with previous records in order to identify the difference. Then the distribution map of this species was formulated through previous floral literatures and voucher specimens. Although the leaf shape which is represented by length/width ratio showed a great variation depending on habitat condition, the rhizome creeping patterns, the presence of hair on the surface leaf margin and perigynium were shown to be good diagnostic characters to define delimitation of species level. The results from molecular study supported $C.$ $ciliatomarginata$ Nakai should be ranked independently on species level.

• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.31-42
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• 2008

It was revaluated about taxonomic position of Bupleurum latissimum Nakai (Apiaceae) by the cluster analysis, the principal component analysis, and the discriminant analysis. Its diagnostic characteristics include bracteole length longer than umbellules, 16-21 pedicel numbers, plant with evergreen leaves, chromosome number 2n = 16 and cauline leaves with auriculate base. It is morphologically similar to B. euphorbioides in having ovate bracteoles, 16-21 pedicel numbers, chromosome number 2n = 16, and cauline leaves with auriculate base, but distinguished from B. longiradiatum with having linear bracteoles, 7-15 pedicel numbers, and chromosome number 2n = 12. Morphological characters support that Bupleurum latissimum Nakai is a Korean endemic species.