• Animal Systematics, Evolution and Diversity
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• 제40권1호
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• pp.35-41
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• 2024

Two species belonging to the genus Gregarinidra Barroso, 1949 (Bryozoa: Gymnolaemata: Flustridae) were first found in this study from Korean waters. Gregarinidra furcula n. sp. is new to science, and G. incrustans (Silén, 1941) is newly added to the Korean fauna. Species belonging to this genus are primarily distinguished by two characteristics: interzooidal avicularia and marginal spines. The pitchforked spines clearly distinguish G. furcula n. sp. from the other Gregarinidra species, and G. incrustans shows the difference by having blunt spines and short-triangular avicularia. Gregarinidra incrustans was known to be distributed only in Japan until this species was reported from the South Sea, Korea, in the present study. This study provides descriptions of two species with detailed illustrations by scanning electron microscopy, distributional data, and a taxonomic key to the Korean Gregarinidra species. With the addition of two species reported herein, four Korean Gregarinidra are recorded: G. corbula Seo, 1996, G. furcula new species, G. incrustans(Silén, 1941), and G. serrata (MacGillivray, 1869). Finally, Gregarinidra totals 10 species worldwide.

• 한국토양비료학회지
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• 제49권2호
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• pp.144-156
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• 2016

Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

• Mycobiology
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• 제50권2호
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• pp.132-141
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• 2022

Amylomyces rouxii is commonly found as amylolytic fungi in tapai fermentation. However, its diversity is rarely reported despite being often used for food production in Southeast Asia. This research aims to analyze the genetic diversity and the distribution pattern of A. rouxii from Ragi tapai in Java Island, Indonesia. We isolated the fungus from samples obtained from Ragi tapai producing centers in Bandung, Sumedang, Muntilan, Blora, Yogyakarta, and Bondowoso. The obtained isolates were molecularly identified based on the ribosomal regions ITS1/ITS2 and D1/D2, then analyzed for phylogenetic tree reconstruction, genetic distance, genetic variation, and haplotype networking. Six isolates showed specific morphological traits of A. rouxii. However, phylogenetic tree reconstruction on the ribosomal genes showed that the isolates were grouped into two different clades related to two species. Clade A included BDG, SMD, and MTL isolates related to A. rouxii, whereas clade B included YOG, BLR, and BDS isolates related to Mucor indicus. The genetic distances between clades for ITS1/ITS2 and D1/D2 were 0.6145 and 0.1556, respectively. In conclusion, we confirmed the genetic diversity of molds from Ragi tapai in Java Island and showed that the isolates are not only related to A. rouxii as reported before.

• The Plant Pathology Journal
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• 제37권6호
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• pp.521-532
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• 2021

Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

• 한국해양학회지:바다
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• 제17권3호
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• pp.160-180
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• 2012

본 연구는 메타게놈 분석법을 기초로 낙동강 하류 담수 환경의 물금과 기수 환경의 을숙도대교 정점에서 채수된 환경 시료내의 진핵 플랑크톤 종다양성 및 군집 구조를 비교 분석하고자 하였다. 수환경 시료에서 추출된 DNA에 대한 environmental Polymerase Chain Reaction(PCR)을 수행하여 18S rDNA 클론라이브러리를 구축하였고, colony PCR, PCR-Restriction Fragment Length Polymorphism(RFLP), 염기서열 결정 및 유사도 분석을 통하여 종다양성을 분석하였다. 물금 및 을숙도대교 정점에서 338개의 클론들을 분석하였고(170 clones, 물금; 168 clones, 을숙도대교), 그 결과 총 74개의 phylotype을 발굴하였다(49개, 물금; 25개, 을숙도대교). 발굴된 phylotype에 대한 계통 분석 결과, Stramenopiles, Cryptophyta, Viridiplantae, Alveolata, Rhizaria, Metazoa 및 Fungi 등의 분류군에 속하는 다양한 생물종이 발굴되었으며, 국내 미기록종 및 신종 후보 가능 생물종과 속(genus)이상의 새로운 분류학적 처리가 필요한 생물종의 존재를 확인하였다. 특히 Stramenopiles의 Pirsonia 및 Alveolata의 Perkinsea에 속하는 phylotypes 등 국내 미기록 생물종을 포함한 숨은 종다양성(cryptic species diversity)의 발굴은 분자모니터링 기법이 낙동강 하구역 수생태계 변화 모니터링을 위한 새로운 유용한 생물학적 정보를 제공할 수 있음을 제시하고 있다.

• Animal Systematics, Evolution and Diversity
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• 제20권2호
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• pp.185-189
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• 2004

대한해협에서 채집한 바다나리류를 동정$\cdot$분류한 결과 바다갯고사리과 (Thalassometridae) 에 속하는 1종의 한국미기록종, 푸른발갯고사리 Parametra orion (A. H. Clark. 1907)로 밝혀져 재기재하고 보고한다. 바다갯고사리과는 한국에서 처음으로 보고된다.

• Animal Systematics, Evolution and Diversity
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• 제23권1호
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• pp.103-105
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• 2007

A taxonomic study on Hoplophrys oatesii Henderson, 1893 was conducted with materials collected from Jejudo Island of Korea by SCUBA diving. H. oatesii is redescribed with some remarks on variations of dactylus and the number of segments of abdomen. The Korean Pisid fauna now consists of seven species of five genera.

• Animal Systematics, Evolution and Diversity
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• 제23권1호
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• pp.91-93
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• 2007

A taxonomic study on the marine sponges was conducted with materials which were collected from Hongdo Island, Korea by SCUBA diving in September 2004. Among them, Higginsia mixta Hentschel, 1912 is newly recorded in the Korean fauna.

• Animal Systematics, Evolution and Diversity
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• 제23권1호
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• pp.57-68
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• 2007

A taxonomic study on the genera Phyllognathopus and Harpacticella has been accomplished as one of the serial researches on the freshwater harpacticoids in South Korea. As a result, four species belonging to the genera are clarified, three of which are new to Korean fauna: P. viguieri (Maupas), P. paludosus Mrazek, and H. paradoxa (Brehm).

• Animal Systematics, Evolution and Diversity
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• 제23권1호
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• pp.45-49
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• 2007

Last instar larvae of Chrysomela cuprea Fabricius, 1775 and C. tremula Fabricius, 1787 are described in detail for the first time. A key to known larvae of Korean Chrysomela, their illustrations and host plants are also given.