• 한국자원식물학회지
• /
• 제30권6호
• /
• pp.640-646
• /
• 2017

In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor $GSK3{\beta}$ but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through $GSK3{\beta}$-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.

• 대한본초학회지
• /
• 제33권5호
• /
• pp.81-88
• /
• 2018

Objectives : The aim of this study was to investigate the protective effects of an aqueous extract from Taxillus chinensis (DC.) Danser (TCE) in Monosodium iodoacetate (MIA)-induced osteoarthritis (OA) rat model. Methods : Sprague Dawley male rats were divided into the following four groups (n=6 per group): Normal (saline control), MIA (MIA-induced OA with vehicle), TCE (MIA-induced with TCE treatment), and IM (MIA-induced with indomethacin treatment). Rats in which OA was induced by MIA were treated with TCE (200 mg/kg) or indomethacin (1 mg/kg) for 4 weeks. Weight-bearing on the hind legs and body weights were measured weekly. At the end of the experiment (3 weeks after MIA injection), serum aspartate aminotransferase and alanine aminotransferase levels were measured to assess the liver toxicity induced by TCE. Its effects on serum inflammatory cytokine levels and tissue histopathology were also evaluated. Results : TCE restored the hind limb weight-bearing distribution. Serum levels of Interleukin 6 (IL-6), Tumor necrosis factor alpha (TNF-${\alpha}$) and Leukotriene B4 (LTB4) were significantly higher in the MIA group than in the Normal group, but serum IL-6 levels were significantly lower in the TCE group. In the TCE group, the synovial membrane was protected in hematoxylin and eosin and Safranin-O staining, respectively. Conclusions : TCE recovered the hind paw weight bearing distribution, inhibited the production of inflammatory cytokine, and protected synovial tissue and cartilage in the OA rat model. Therefore, TCE appears to be an effective therapeutic agent for treating OA and OA-related symptoms.

• 한국식품과학회지
• /
• 제29권4호
• /
• pp.671-679
• /
• 1997

국내에서 재배, 유통되고 있는 약초 일반성분 및 무기질 함량을 분석하였다. 건물기준으로 조회분은 오가피, 쇠비름, 사상자가 각각 22.4, 20.6, 15.0%로 다량 함유하고 있었고, 조단백은 산조인, 택사, 정력자, 결명자가 각각 39.9, 30.6, 28.8, 27.5%로 다량 함유하고 있었다. 조지방은 산조인, 창이자, 소자, 동과자 순으로 각각 27.9, 24.0, 22.8, 22.4%, 조섬유는 만형자, 영지, 송절, 접골목 순으로 각각 59.5, 53.9, 46.4, 41.2% 그리고 가용성 무질소물은 복령, 천마, 초용담, 지황이 각각 91.2, 87.9, 86.3, 86.0%로 다량 함유하고 있는 것으로 분석되었다. 인은 상기생, 동규자, 연자육이 각각 100 g당 1,485.9, 1,149.5, 985.0 mg로 다량 함유한 것으로 분석되었고, 마그네슘은 삼백초, 단삼, 칡이 각각 728.0, 582.0, 464.9 mg로 다량 함유하였다. 칼슘은 승마, 오가피, 건칠이 각각 8,506.6, 7,106.5, 3,010.2 mg로 측정되었으며, 칼륨은 송이, 어성초, 우슬이 각각 5,613.5, 5,353.5, 4,665.7 mg였다. 또한 나트륨의 경우 육종용, 두충, 매실이 각각 2,064.5, 595.3, 291.4 mg, 철은 대계근, 초용담, 속단이 각각 307.4, 233.0, 207.6 mg로 나타났다.