• The Korean Journal of Pain
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• 제21권2호
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• pp.112-118
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• 2008

Background: This study was conducted to quantitatively analyze proteins associated with the calcitonin gene-related peptide (CGRP) in cerebrospinal fluid (CSF) that was obtained from a rat model of chronic neuropathic pain following administration of intrathecal $CGRP_{8-37}$. Methods: Male Sprague-Dawley rats (100-150 g, 5-6 wks) were divided into two groups, sham controls and neuropathic pain models. At the time of operation for neuropathic pain model, an intrathecal catheter was threaded through the intrathecal space. At 1 or 2 wks after the operation (maximum pain state), a test dose of 1, 5, 10, or 50 nM of $CGRP_{8-37}$ was injected into the intrathecal catheter and the CSF was then aspirated. Conventional proteomics to evaluate the CSF were then performed using high resolution 2-D, gel electrophoresis followed by computational image analysis and protein identification by mass spectrometry. Results: Treatment with $CGRP_{8-37}$ effectively alleviated mechanical allodynia in a dose dependent manner. The most effective response was obtained when a dose of 50 nM was administered, but significant differences were obtained following administration of only 5 nM $CGRP_{8-37}$. Furthermore, the results of the proteomic analysis were consistent with the experimental results. Specially we detected 30 differentially expressed spots in 7 images when 2-D gel electrophoresis was conducted. The intensity of 6 of these spots (spot number: 20 and 26-30) was found decrease the $CGRP_{8-37}$ dose increased; therefore, these spots were evaluated by mass spectrometry. This analysis identified 2 different proteins, CGRP (spot numbers: 26-30) and neurotensin-related peptide (spot number: 20). Conclusions: The results of this study suggest that CGRP plays a role in chronic central neuropathic pain and is a major target of chronic neuropathic pain management.

• 도시과학
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• 제7권2호
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• pp.53-60
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• 2018

Digital Twin is a technology that creates a photocopy of real-world objects on a computer and analyzes the past and present operational status by fusing the structure, context, and operation of various physical systems with property information, and predicts the future society's countermeasures. In particular, 3D rendering technology (UAS, LiDAR, GNSS, etc.) is a core technology in digital twin. so, the research and application are actively performed in the industry in recent years. However, UAS (Unmanned Aerial System) and LiDAR (Light Detection And Ranging) have to be solved by compensating blind spot which is not reconstructed according to the object shape. In addition, the terrestrial LiDAR can acquire the point cloud of the object more precisely and quickly at a short distance, but a blind spot is generated at the upper part of the object, thereby imposing restrictions on the forward digital twin modeling. The UAS is capable of modeling a specific range of objects with high accuracy by using high resolution images at low altitudes, and has the advantage of generating a high density point group based on SfM (Structure-from-Motion) image analysis technology. However, It is relatively far from the target LiDAR than the terrestrial LiDAR, and it takes time to analyze the image. In particular, it is necessary to reduce the accuracy of the side part and compensate the blind spot. By re-optimizing it after fusion with UAS and Terrestrial LiDAR, the residual error of each modeling method was compensated and the mutual correction result was obtained. The accuracy of fusion-based 3D model is less than 1cm and it is expected to be useful for digital twin construction.

• 한국운동역학회지
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• 제20권4호
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• pp.437-445
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• 2010

This study was aimed at comparing selected joint angles at the golf address stance by categorizing three different body types. 43 elite male golfers were selected and 9 of them turned out to be the ectoderm while 24 of them to be the mesoderm. The remaining 10 subjects were the endoderm. The measurement was carried out at the address stance with the number 7 iron and the driver. The result showed that the angle of trunk flexion did not different among body types. The trunk tilting angle became more inclined to the right side, which confirmed the guidelines from most of golf lesson books, for bigger people since they tended to put more weight on the right foot. The angle of both knees showed similar but the right knee was bent more than the left knee. The target direction and body alignment faced more to the left side than the target spot because of the influence of open stance and natural aiming position. It seems that pelvis and knees turned a little bit more to the right side than the target direction in order to maintain the parallel. Overall, significant differences among body types were found at the trunk tilting angle and pelvis-target alignment and golf address configuration can be differentiate by these factors.

• 벤처창업연구
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• 제7권2호
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• pp.151-156
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• 2012

본 논문에서 우리는 동시에 볼 수 있는 수정된 3개의 관리도를 제안하였다. 첫번째 곤리도는 샘플 데이터로만 그려진 관리도이다. 두번째 관리도는 처음 관리도에 상하한 규격을 포함한 관리도이다. 세번째 관리도는 목표값이 포함되고 오랜 기간 동안의 데이터로 계산된 알려진 평균과 표준편차로 계산된 상 하한 관리선이 포함된 관리도이다. 3개의 관리도를 동시에 보면 즉시 공정의 문제점을 알아낼 수 있는 정점이 있다. 이 논문에서 제안된 방법이 현장에서 활용되어 도움을 주길 희망한다.

• 한국소음진동공학회논문집
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• 제13권10호
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• pp.785-790
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• 2003

This paper presents an approach to optimally design the air-hearing surface (ABS) of the optical flying head for near-field recording technology (NFR) NFR is an optical recording technology using very small beam spot size by overcoming the limit of beam diffraction. One of the most important problems in NFR Is a head disk interface (HDI) issue over the recording band during the operation. A multi-criteria optimization problem is formulated to enhance the flying performances over the entire recording band during the steady state. The optimal solution of the slider, whose target flying height is 50 nm, is automatically obtained. The flying height during the steady state operation becomes closer to the target values than those for the Initial one. The pitch and roll angles are also kept within suitable ranges over the recording band. Especially. all of the all-hearing stiffness are drastically increased by the optimized geometry of the air hearing surface.

• 농업과학연구
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• 제17권2호
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• pp.82-86
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• 1990

토마토잎에 발생한 갈잭의 둥근 윤문병반에서 분리한 Carynespara sp.는 C. cassiicola로 동정되었으며 이 균을 접종 실험한 결과 토마토에 병원성을 나타내었다. 이 균의 생장적온은 $27^{\circ}C$였으며 $19^{\circ}C$와 $35^{\circ}C$에서는 균총생장이 저조하였다. 포자형성은 potato dextrose malt agar에 서 매우 양호하였으며 V-8 juice agar와 potato dextrose agar에서는 중간정도였다. 병원균을 인공배양하여 토마토 건전식물체에 분무접종하였을 때 자연상태에서와 유사한 갈색의 윤문을 형성하는 병징을 나타내었다.

• 한국기계가공학회지
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• 제15권4호
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• pp.46-50
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• 2016

A laser head was designed for micro-scale patterning and joining applications. The target feature size of the pattern was $100{\mu}m$, and optics were designed to perform the target. Two singlet lenses were combined to minimize the chromatic aberration, and the geometry of the lenses was calculated by using the raytracing method with a commercial software program. As a restriction of lens design, the focal length was set at 100mm, and the maximum diameter of the lens or beam size was limited to 10mm for the assembly in the limited cage size. The maximum temperatures were calculated to be $1367^{\circ}C$, $1508^{\circ}C$, and $1905^{\circ}C$ for 10, 12, and 15 Watts of power, respectively. A specially designed laser head was used to compensate for the distance between the object and the lens. The detailed design mechanism and 3D data were presented. The optics design and detailed performance of the lens were analyzed by using MTF and spot diagram calculation.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.22-26
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• 2002

There are a number of ways in which environmental microbiology and microbial ecology will benefit from DNA micro array technology. These include community genome arrays, SSU rDNA arrays, environmental functional gene arrays, population biology arrays, and there are clearly more different applications of microarray technology that can be applied to relevant problems in environmental microbiology. Two types of the applications, bacterial identification chip and functional gene detection chip, will be presented. For the bacterial identification chip, a new approach employing random genome fragments that eliminates the disadvantages of traditional DNA-DNA hybridization is proposed to identify and type bacteria based on genomic DNA-DNA similarity. Bacterial genomes are fragmented randomly, and representative fragments are spotted on a glass slide and then hybridized to test genomes. Resulting hybridization profiles are used in statistical procedures to identify test strains. Second, the direct binding version of microarray with a different array design and hybridization scheme is proposed to quantify target genes in environmental samples. Reference DNA was employed to normalize variations in spot size and hybridization. The approach for designing quantitative microarrays and the inferred equation from this study provide a simple and convenient way to estimate the target gene concentration from the hybridization signal ratio.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.69-75
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• 2012

Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof-care applications.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.667-668
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• 2015

While the plant projects grow bigger and global attention to the plant is increasing, efficient space arrangement is not working in plant project because of the complex structure in installing the equipment unlike the construction project. In addition to this, presently, problem in installation process caused by the disagreement between floor plan and real spot is rising. Therefore the target of this research is to solve the problems and reaction differences, caused by changing the space arrangement in installing the equipment of plant construction. And this research suggests the equipment arrangement method for construction and related processes. To solve the problem, 3D cloud point data of space and equipment is collected by 3D laser scanning and the space matching is operated. In processing the space matching, data is simplified by applying the octree algorithm. This research simplifies the 3D configuration data acquired by 3D scanner equipment through the octree algorithm, and by comparing this data, identifies the space for target equipment, and finally suggests the algorithm that makes the auto space arrangement of equipment possible in construction site and also suggests the process to actualize this algorithm.