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http://dx.doi.org/10.5012/bkcs.2012.33.1.69

Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips  

Zhang, Xuzhi (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Ji, Xinming (The State Key Laboratory of ASIC & System, Department of Microelectronics, Fudan University)
Cui, Zhengguo (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Yang, Bing (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Huang, Jie (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Publication Information
Bulletin of the Korean Chemical Society / v.33, no.1, 2012 , pp. 69-75 More about this Journal
Abstract
Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof-care applications.
Keywords
DNA chips; WSSV gene; Multi-channel electrochemical detection; Electroanalysis; Loop-mediated isothermal amplification;
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