• 제목/요약/키워드: Target spot

검색결과 162건 처리시간 0.011초

Quality Control Probes for Spot-Uniformity and Quantitative Analysis of Oligonucleotide Array

  • Jang, Hyun-Jung;Cho, Mong;Kim, Hyung-Hoi;Kim, Cheol-Min;Park, Hee-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제19권7호
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    • pp.658-665
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    • 2009
  • Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.

SPOT 위성영상의 스트립 센서모델링을 이용한 비접근지역 위치결정 연구 (Target Positioning in Remote Area Using Strip Sensor Modeling of SPOT Imagery)

  • 김만조;황치정
    • 한국군사과학기술학회지
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    • 제15권2호
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    • pp.155-160
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    • 2012
  • In this paper, a strip modeling method is developed for the acquisition of target positions in remote area and validated using the imagery of SPOT satellite. This method utilizes the parameters given in header files and constructs a camera model without ground control points. In most cases, the root mean squared error of check points is less than pixel size with one ground control point. The model error of reference image is evaluated using ground control points and used to remove the model error of target images acquired along the same satellite orbit, which enables one to calculate target positions in remote area where no ground control points are available.

수질오염총량제 대응을 위한 경남 하천 수질의 시공간 경향성 분석 (Analyzing the Spatio-temporal Trend in TMDL Water Quality for Gyeongnam Using Emerging Hot Spot Analysis)

  • 선단비;김지호;김상민;장민원
    • 농촌계획
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    • 제26권4호
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    • pp.53-65
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    • 2020
  • This study aimed to provide a basic information for managing the water quality of national and regional 1st rivers in Gyeongnam by analyzing the emerging hot spot patterns in BOD, T-P, and TOC, and by grouping the changing trends into clusters. The emerging hot spot analysis for each water quality item was implemented in ArcGIS Desktop with monthly water quality data from 96 water environmental monitoring stations in Gyeongnam, and then four patterns of water quality change were classified by the K-mean cluster analysis. As for BOD, persistent cold spot pattern covered about 42.9% of target rivers, and T-P concentration tended to be low or be getting lower at over 70% of target rivers. While, for TOC, about 70% of target rivers resulted in oscillating hot spots. In addition, the cluster analysis showed that the downstream of Nakdong river had the top priority in terms of water quality management because of the increasing concentration for all the three water quality.

Corynespora cassiicola에 의한 로즈마리 점무늬병 (Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea)

  • 이왕휴;한상준;최인영
    • 식물병연구
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    • 제19권1호
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    • pp.55-59
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    • 2013
  • 이 연구는 국내에 보고되지 않은 로즈마리 점무늬병이 남원과 전주의 재배 온실에서 발생하여 구명하고자 실험하였다. 이 병은 주로 고온 다습한 시기인 장마끝 무렵에 발생이 많았다. 로즈마리의 잎과 줄기에 검은점무늬(직경 3-5 mm) 및 시들음 증상이 나타났다. 병든 조직을 습실 처리하면 분생자경과 분생포자가 형성되었다. 로즈마리 점무늬병균의 분생포자는 체인상의 가늘고 긴 원통형으로, 길이는 $55-275{\times}7-14{\mu}m$이었다. 분생포자는 분생자경 위에 형성되었고, 분생포자의 위격벽의 수는 8-10개이었다. 또한 생장적온은 감자한천 배지와 암흑조건에서 $30^{\circ}C$이었다. 포트 식물에 병원성 검정결과 분리한 병원균은 접종 3일 후에 잎과 줄기에서 자연 병징과 같은 점무늬 및 시들음 증상이 재현되었고, 병든 부위에서 동일한 균이 재 분리되었다. 따라서 병원성이 있음이 확인되었다. rDNA ITS 영역의 염기서열 분석결과 로즈마리에 점무늬병을 일으키는 병원균은 Corynespora cassiicola로 GenBank accession number JQ595296, JQ595297, FJ852715, AY238606와 염기서열이 100% 일치하였고, C. cassiicola와 같은 계통군에 속하였다. 따라서, 로즈마리 잎 및 가지 점무늬병균은 C. cassiicola로 보고하고자 한다.

비젼을 이용한 카메라 렌즈 이물질 검사 시스템 개발 (Spot insepction System for Camera Target Lens using the Computer Aided Vision System)

  • 이일환;안우정;박희재;황두현;김왕도
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1996년도 춘계학술대회 논문집
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    • pp.271-275
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    • 1996
  • In this paper, an automatic spot inspection system has been developed for camera target lens using the computer aided vision system. The developed system comprises: light source, magnifying optics, vision camera, XY robot, and a PC. An efficient algotithm for the spot detection has been implemented, thus up tof ew micrometer size spots can be effectively identified in real time. The developed system has been fully interfaced with XY robot systenm, PLCs, thus the practical spot inspection system has been implemented. The system has been applied to a practical camera manufacturing process, and showed its efficiency.

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대향2문조사시 Target Volume의 위치에 따른 Beam Weight의 최적화

  • 이진국;김지한;임익수;최영헌
    • 대한방사선치료학회지
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    • 제5권1호
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    • pp.68-73
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    • 1992
  • If the same weight is used in parallel opposed 6 and 10 MV x-ray beams, the lowest dose is achieved at SAD. Therefore, dose homogeneity in the target volume is decreased when SAD is taken at center of target volume than center of phantom or patient. With Standard deviation of ${\pm}6\%$ that repuesented the dose homogeneity in tarhet volume, we studied the optimized beam weights at which hot spot dose was least in parallel opposed beams. The optimized beam weights that maximally decrease the hot spot dose, wer 1.29, 1.19, 2.71, 3.50, and 4.70 in 6 MV x-ray and 1.25, 1.53, 1.90, 2.36, 3.01, and 3.7 in 10 MV x-ray, reapectively, when center of target volume was changed to 2,4,6,8,10, and 12cm from center plan of phantom along the centeral axis of beams.

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양극의 경사각 효과에 따른 조사야 X-선 강도 분포 (Distribution of X-ray Strength in Exposure Field Caused by Heel Effect)

  • 장근조;김남훈;이준행;이상복
    • 한국방사선학회논문지
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    • 제5권5호
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    • pp.223-229
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    • 2011
  • X선은 X선관 내 음극측 전자(electron)를 빠른 속도로 가속시킨 다음 진행하는 전자의 흐름을 저지극(target)에서 차단시킬 때 에너지의 변환을 일으켜 발생한다. 가속된 고속의 전자가 저지면에 충돌하는 실제면적을 실초점(actual focal spot)이라 하고, 실초점의 크기를 X선이 나오는 방향인 중심선(central ray)측에서 관측할 경우 축소되어 작게 보이는데 이때의 초점을 실효초점(effective focal spot)이라고 한다. X선관 방사각에 따라 음극 측의 강도가 양극 측 보다 높게 나타나 X선 강도가 균등하지 않다. 이러한 효과를 경사각 효과(heel effect)라고 하며, 경사각 효과로 인하여 환자가 받는 피폭의 정도는 양극의 각도, 즉 실효초점의 크기에 따라 달라지게 된다. 본 논문에서는 실효초점의 크기와 그에 따른 환자 피폭선량의 상관관계를 알아보고 실효초점의 크기에 따른 균질선량 분포를 위한 효과적인 조사야를 제시하고자 한다. 결론적으로 초점크기에 따라서 평균적으로 -8cm ~ 0cm 범위에서 효과적인 조사야 범위를 찾을 수 있었고, 평균 선량률은 0.019 R/min이 나왔다. 이 범위를 이용하면 환자에게는 적은 피폭선량으로 균등한 흑화도 및 해상력을 가진 영상을 얻을 수 있을 것이다.

Occurrence of Target Leaf Spot of Red and White Clovers Caused by Stemphylium sarciniforme in Korea

  • Cho, Hye-Sun;Yu, Seung-Hun
    • The Plant Pathology Journal
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    • 제16권6호
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    • pp.328-330
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    • 2000
  • A terget leaf spot of red and white clovers was observed during 1998-2000 growing seasons in several fields of Chungnam and Chungbuk provinces in Korea. Lesions were circular to oval in outline, brown to dark brown, ranging from a pinpoint to 3-4 mm in diameter, often concentrically zonate and presenting a target effect. A fungus associated with the disease was identified as Stemphylium sarciniforme based on the morphological characteristics of the conidiophores and conidia. The fungus was pathogenic on red and white clovers in the inoculation test. This is the first record of a terget leaf spot of red and white clovers in Korea.

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동판을 이용한 Spot 촬영법에 관한 새로운 시도 (A New Attempt on Spot Radiography Using Cu Mask)

  • 김정민;강홍석;이인자;신화수;허준
    • 대한방사선기술학회지:방사선기술과학
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    • 제10권1호
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    • pp.37-41
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    • 1987
  • Authors could recognize surrounding areas induding target area by the observable film density with spot radiography using Cu mask and also, radiographic images maintained high quality. Spot radiography has been utilized for a long time for the purpose of observing detail image by the control of scattered radiation. But it has been difficult to know body part in general spot radiography. Therefore it is thought that attempt to use Cu mask in spot radiography is very advantageous in a clinical examinations.

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중앙은행의 OTC 통화옵션시장을 활용한 외환시장 개입 전략에 관한 연구 (A Study on the Central Bank's Foreign Exchange Market Intervention Strategies with OTC Currency Option Market)

  • 박재관
    • 무역학회지
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    • 제47권2호
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    • pp.103-120
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    • 2022
  • This paper studies the possibility of options as an instrument for central bank to intervene foreign exchange market. As opposed to spot transaction or forward transaction, which impacts spot exchange rate only once, currency options can continuously resist a directional speculative pressure on spot market due to the dynamic delta hedging of OTC currency options market maker. This research also analyzes whether and how central banks can use currency options to lower exchange rate volatility and maintain (implicit) target zones in foreign exchange markets. It argues that short position rather than long position in options will result in market makers dynamically hedging their long option exposure in a stabilizing manner, consistent with the first objective. Selling a "Strangle" allows a central bank to increase the credibility of its commitment to a target zone, and could have a lower expected cost than spot market interventions. However, this strategy also exposes the central bank to an unlimited loss potential. Therefore these kinds of intervention strategies must be used in the short run and temporarily.