• Title/Summary/Keyword: Target spot

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Quality Control Probes for Spot-Uniformity and Quantitative Analysis of Oligonucleotide Array

  • Jang, Hyun-Jung;Cho, Mong;Kim, Hyung-Hoi;Kim, Cheol-Min;Park, Hee-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.19 no.7
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    • pp.658-665
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    • 2009
  • Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.

Target Positioning in Remote Area Using Strip Sensor Modeling of SPOT Imagery (SPOT 위성영상의 스트립 센서모델링을 이용한 비접근지역 위치결정 연구)

  • Kim, Man-Jo;Hwang, Chi-Jung
    • Journal of the Korea Institute of Military Science and Technology
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    • v.15 no.2
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    • pp.155-160
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    • 2012
  • In this paper, a strip modeling method is developed for the acquisition of target positions in remote area and validated using the imagery of SPOT satellite. This method utilizes the parameters given in header files and constructs a camera model without ground control points. In most cases, the root mean squared error of check points is less than pixel size with one ground control point. The model error of reference image is evaluated using ground control points and used to remove the model error of target images acquired along the same satellite orbit, which enables one to calculate target positions in remote area where no ground control points are available.

Analyzing the Spatio-temporal Trend in TMDL Water Quality for Gyeongnam Using Emerging Hot Spot Analysis (수질오염총량제 대응을 위한 경남 하천 수질의 시공간 경향성 분석)

  • Sun, Danbee;Kim, Jiho;Kim, Sangmin;Jang, Min-Won
    • Journal of Korean Society of Rural Planning
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    • v.26 no.4
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    • pp.53-65
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    • 2020
  • This study aimed to provide a basic information for managing the water quality of national and regional 1st rivers in Gyeongnam by analyzing the emerging hot spot patterns in BOD, T-P, and TOC, and by grouping the changing trends into clusters. The emerging hot spot analysis for each water quality item was implemented in ArcGIS Desktop with monthly water quality data from 96 water environmental monitoring stations in Gyeongnam, and then four patterns of water quality change were classified by the K-mean cluster analysis. As for BOD, persistent cold spot pattern covered about 42.9% of target rivers, and T-P concentration tended to be low or be getting lower at over 70% of target rivers. While, for TOC, about 70% of target rivers resulted in oscillating hot spots. In addition, the cluster analysis showed that the downstream of Nakdong river had the top priority in terms of water quality management because of the increasing concentration for all the three water quality.

Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea (Corynespora cassiicola에 의한 로즈마리 점무늬병)

  • Lee, Wang-Hyu;Han, Sang-Jun;Choi, In-Young
    • Research in Plant Disease
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    • v.19 no.1
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    • pp.55-59
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    • 2013
  • The purpose of this experiment was to investigate the development of new spot disease on the leaf and stem of rosemary (Rosmarinus officinalis) in commercial greenhouses at Jeonju and Namwon in Korea. Incidence of target spot on rosemary was higher at the end of the rainy season with high humidity. Those symptoms were black ring spots (3-5 mm in diameter) and withering on green leaves and stems. Conidiophores and conidia were formed on the infected tissue in moist chamber and conidia were shown as the cylindrical and oval types in chain, ranged from 55 to $275{\mu}m$ in length, and 7 to $14{\mu}m$ in width. Conidia with eight to ten pseudosepta were formed on the conidiapore. The optimum growth temperature of isolates was $30^{\circ}C$ on the PDA medium under the dark condition. In the pathogenesis test, the target spot and withering symptoms were appeared on the leaves and stems 3 days after inoculation showing similar symptoms compared to those of in nature. The same fungus was re-isolated from infected lesion, indicating that Corynespora cassiicola caused leaf target spot and twig blight on rosemary. The rDNA ITS nucleotide sequences of the pure cultured isolate from the diseased area on rosemary showed 100% similarity to the sequences of C. cassiicola available in the GenBank database (JQ595296, JQ595297, FJ852715 and AY238606). Therefore, we report that the target spot of leaves and stems in rosemary was caused by C. cassiicola.

Spot insepction System for Camera Target Lens using the Computer Aided Vision System (비젼을 이용한 카메라 렌즈 이물질 검사 시스템 개발)

  • 이일환;안우정;박희재;황두현;김왕도
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.271-275
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    • 1996
  • In this paper, an automatic spot inspection system has been developed for camera target lens using the computer aided vision system. The developed system comprises: light source, magnifying optics, vision camera, XY robot, and a PC. An efficient algotithm for the spot detection has been implemented, thus up tof ew micrometer size spots can be effectively identified in real time. The developed system has been fully interfaced with XY robot systenm, PLCs, thus the practical spot inspection system has been implemented. The system has been applied to a practical camera manufacturing process, and showed its efficiency.

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대향2문조사시 Target Volume의 위치에 따른 Beam Weight의 최적화

  • Lee Jin Guk;Kim Ji Han;Im Ik Su;Choe Yeong Heon
    • The Journal of Korean Society for Radiation Therapy
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    • v.5 no.1
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    • pp.68-73
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    • 1992
  • If the same weight is used in parallel opposed 6 and 10 MV x-ray beams, the lowest dose is achieved at SAD. Therefore, dose homogeneity in the target volume is decreased when SAD is taken at center of target volume than center of phantom or patient. With Standard deviation of ${\pm}6\%$ that repuesented the dose homogeneity in tarhet volume, we studied the optimized beam weights at which hot spot dose was least in parallel opposed beams. The optimized beam weights that maximally decrease the hot spot dose, wer 1.29, 1.19, 2.71, 3.50, and 4.70 in 6 MV x-ray and 1.25, 1.53, 1.90, 2.36, 3.01, and 3.7 in 10 MV x-ray, reapectively, when center of target volume was changed to 2,4,6,8,10, and 12cm from center plan of phantom along the centeral axis of beams.

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Distribution of X-ray Strength in Exposure Field Caused by Heel Effect (양극의 경사각 효과에 따른 조사야 X-선 강도 분포)

  • Jang, Keun-Jo;Kim, Nam-Hun;Lee, Jun-Haeng;Lee, Sang-Bock
    • Journal of the Korean Society of Radiology
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    • v.5 no.5
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    • pp.223-229
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    • 2011
  • When negative electron in x-ray tube is accelerated in to a high speed and then the currency of the electron is blocked by the target, x-ray happens by the conversion of the energy. The real area where the fast accelerated electron collides to a target area is called actual focal spot. When the string focused size is observed at the central ray side, where the direction x-ray comes out, the size seems to be reduced. This focus is called effective focal spot. According to radiation angle of x-rays tube, the degree of the negative pole side presents higher value than inclination, the amount of exposed radiation that patient receives differs by the angle of positive pole, which means effective focal spot is the variable. This paper presents the correlation between size of effective focal spot and amount of exposed radiation to the patient by it, and effective research for homogenized dose dispersion by the size of effective focal spot. In conclusion, following the focal size, effective range which was -8cm ~ 0 cm on average, was found and average dose rate was 0.019 R/min. Through this range, for patients with small radiation exposure, image with good density and resolution in aspect of diagnosing will be able to be obtained.

Occurrence of Target Leaf Spot of Red and White Clovers Caused by Stemphylium sarciniforme in Korea

  • Cho, Hye-Sun;Yu, Seung-Hun
    • The Plant Pathology Journal
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    • v.16 no.6
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    • pp.328-330
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    • 2000
  • A terget leaf spot of red and white clovers was observed during 1998-2000 growing seasons in several fields of Chungnam and Chungbuk provinces in Korea. Lesions were circular to oval in outline, brown to dark brown, ranging from a pinpoint to 3-4 mm in diameter, often concentrically zonate and presenting a target effect. A fungus associated with the disease was identified as Stemphylium sarciniforme based on the morphological characteristics of the conidiophores and conidia. The fungus was pathogenic on red and white clovers in the inoculation test. This is the first record of a terget leaf spot of red and white clovers in Korea.

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A New Attempt on Spot Radiography Using Cu Mask (동판을 이용한 Spot 촬영법에 관한 새로운 시도)

  • Kim, Chung-Min;Kang, Hong-Seok;Lee, In-Ja;Shin, Wha-Soo;Huh, Joon
    • Journal of radiological science and technology
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    • v.10 no.1
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    • pp.37-41
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    • 1987
  • Authors could recognize surrounding areas induding target area by the observable film density with spot radiography using Cu mask and also, radiographic images maintained high quality. Spot radiography has been utilized for a long time for the purpose of observing detail image by the control of scattered radiation. But it has been difficult to know body part in general spot radiography. Therefore it is thought that attempt to use Cu mask in spot radiography is very advantageous in a clinical examinations.

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A Study on the Central Bank's Foreign Exchange Market Intervention Strategies with OTC Currency Option Market (중앙은행의 OTC 통화옵션시장을 활용한 외환시장 개입 전략에 관한 연구)

  • Jae-Kwan Park
    • Korea Trade Review
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    • v.47 no.2
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    • pp.103-120
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    • 2022
  • This paper studies the possibility of options as an instrument for central bank to intervene foreign exchange market. As opposed to spot transaction or forward transaction, which impacts spot exchange rate only once, currency options can continuously resist a directional speculative pressure on spot market due to the dynamic delta hedging of OTC currency options market maker. This research also analyzes whether and how central banks can use currency options to lower exchange rate volatility and maintain (implicit) target zones in foreign exchange markets. It argues that short position rather than long position in options will result in market makers dynamically hedging their long option exposure in a stabilizing manner, consistent with the first objective. Selling a "Strangle" allows a central bank to increase the credibility of its commitment to a target zone, and could have a lower expected cost than spot market interventions. However, this strategy also exposes the central bank to an unlimited loss potential. Therefore these kinds of intervention strategies must be used in the short run and temporarily.