• Journal of Microbiology and Biotechnology
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• 제29권4호
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• pp.577-586
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• 2019

The engineered Aspergillus oryzae has a high NADPH demand for xylose utilization and overproduction of target metabolites. Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) is one of two key enzymes in the oxidative part of the pentose phosphate pathway, and is also the main enzyme involved in NADPH regeneration. The open reading frame and cDNA of the putative A. oryzae G6PDH (AoG6PDH) were obtained, followed by heterogeneous expression in Escherichia coli and purification as a his6-tagged protein. The purified protein was characterized to be in possession of G6PDH activity with a molecular mass of 118.0 kDa. The enzyme displayed maximal activity at pH 7.5 and the optimal temperature was $50^{\circ}C$. This enzyme also had a half-life of 33.3 min at $40^{\circ}C$. Kinetics assay showed that AoG6PDH was strictly dependent on $NADP^+$ ($K_m=6.3{\mu}M$, $k_{cat}=1000.0s^{-1}$, $k_{cat}/K_m=158.7s^{-1}{\cdot}{\mu}M^{-1}$) as cofactor. The $K_m$ and $k_{cat}/K_m$ values of glucose-6-phosphate were $109.7s^{-1}{\cdot}{\mu}M^{-1}$ and $9.1s^{-1}{\cdot}{\mu}M^{-1}$ respectively. Initial velocity and product inhibition analyses indicated the catalytic reaction followed a two-substrate, steady-state, ordered BiBi mechanism, where $NADP^+$ was the first substrate bound to the enzyme and NADPH was the second product released from the catalytic complex. The established kinetic model could be applied in further regulation of the pentose phosphate pathway and NADPH regeneration of A. oryzae to improve its xylose utilization and yields of valued metabolites.

• 천문학회보
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• 제44권1호
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• pp.84.2-84.2
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• 2019

We present the absolute properties of the double-lined eclipsing binary KIC 6206751 exhibiting multiperiodic pulsations. The Kepler light curve of this system was simultaneously solved with the previously published radial-velocity data. The results indicate that the binary star is a short-period semi-detached system with fundamental parameters of $M_1=1.66{\pm}0.04M_{\odot}$, $M_2=0.215{\pm}0.006M_{\odot}$, $R_1=1.53{\pm}0.02R_{\odot}$, $R_2=1.33{\pm}0.02R_{\odot}$, $L_1=5.0{\pm}0.6L_{\odot}$, and $L_2=0.96{\pm}0.09L_{\odot}$. We applied multiple frequency analyses to the eclipse-subtracted light residuals and detected the 42 frequencies below $2.5days^{-1}$. Among these, three independent frequencies of $f_2$, $f_3$, and $f_4$ can be identified as high-order ($38{\leq}n{\leq}40$) low-degree (l=2) gravity-mode oscillations, whereas the other frequencies may be orbital harmonics and combination terms. The ratios between the orbital frequency and the pulsation frequencies are $f_{orb}:f_{2-4}{\simeq}2:3$, which implies that the ${\gamma}$ Dor pulsations of the detached primary star may be excited by the tidal interaction of the secondary companion. The short orbital period, and the low mass ratio and $M_2$ demonstrate that KIC 6206751 is an R CMa-type star, which is most likely evolving into an EL CVn star. Of seven well-studied R CMa-type stars, our program target is the only eclipsing binary with a ${\gamma}$ Dor pulsating component.

• 한국물환경학회지
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• 제35권5호
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• pp.409-417
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• 2019

In this study, a $480m^3/day$ pilot device was constructed through laboratory experiments based on the Ministry of Environment manual. The purpose of this study was to analyze the characteristics of CSO treatment and backwashing characteristics by applying the pilot device to the field. The purpose of this study was to acquire the basic data necessary for the design and operation management of the real scale filtration type non-point pollution control system. The filtration was conducted while maintaining the linear velocity of 20m/hour. The CSO treatment efficiencies of the pilot devices were 0.4-76.1%(mean 49.0 %), SS 51.4-91.6%(mean 77.8%), COD 22.2-59.4% (mean 38.3%) and TP 14.5-52.6%(mean 38.1%),respectively. The correlation coefficient between SS and the turbidity of influent water was 0.90, higher than that of CSO. To operate the treatment system effectively, the turbidity can be easily measured in real time as the monitoring item is the most appropriate because SS is the main target substance of the non-point source. As a result of analyzing the adsorbent treatment characteristics of PP filter material applied to this pilot device, the average particle diameter range of influent was $4.6-40.1{\mu}m$(mean $21.2{\mu}m$) and the treated water was $0.9-24.5{\mu}m$(mean $6.4{\mu}m$), respectively. Particles of approximately 10m or less are leached out, and so it is necessary to compensate for the raw water containing micro particulate matter.

• Advances in nano research
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• 제10권2호
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• pp.151-163
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• 2021

The main target of this study is to investigate nonlinear transient responses of moving polymer nano-size plates fortified by means of Graphene Platelets (GPLs) and resting on a Winkler-Pasternak foundation under a transverse pressure force and a temperature variation. Two graphene spreading forms dispersed through the plate thickness are studied, and the Halpin-Tsai micro-mechanics model is used to obtain the effective Young's modulus. Furthermore, the rule of mixture is employed to calculate the effective mass density and Poisson's ratio. In accordance with the first order shear deformation and von Karman theory for nonlinear systems, the kinematic equations are derived, and then nonlocal strain gradient scheme is used to reflect the effects of nonlocal and strain gradient parameters on small-size objects. Afterwards, a combined approach, kinetic dynamic relaxation method accompanied by Newmark technique, is hired for solving the time-varying equation sets, and Fortran program is developed to generate the numerical results. The accuracy of the current model is verified by comparative studies with available results in the literature. Finally, a parametric study is carried out to explore the effects of GPL's weight fractions and dispersion patterns, edge conditions, softening and hardening factors, the temperature change, the velocity of moving nanoplate and elastic foundation stiffness on the dynamic response of the structure. The result illustrates that the effects of nonlocality and strain gradient parameters are more remarkable in the higher magnitudes of the nanoplate speed.

• 한국지진공학회논문집
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• 제25권5호
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• pp.223-232
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• 2021

The spatial variation characteristics of seismic motions at the nuclear power plant's site and structures were analyzed using earthquake records obtained at the Fukushima nuclear power plant during the Great East Japan Earthquake. The ground responses amplified as they approached the soil surface from the lower rock surface, and the amplification occurred intensively at about 50 m near the ground. Due to the soil layer's nonlinear characteristics caused by the strong seismic motion, the ground's natural frequency derived from the response spectrum ratio appeared to be smaller than that calculated from the shear wave velocity profile. The spatial variation of the peak ground acceleration at the ground surface of the power plant site showed a significant difference of about 0.6 g at the maximum. As a result of comparing the response spectrums at the basement of the structure with the design response spectrum, there was a large variability by each power plant unit. The difference was more significant in the Fukushima Daiichi site record, which showed larger peak ground acceleration at the surface. The earthquake motions input to the basement of the structure amplified according to the structure's height. The natural frequency obtained from the recorded results was lower than that indicated in the previous research. Also, the floor response spectrum change according to the location at the same height was investigated. The vertical response on the foundation surface showed a significant difference in spectral acceleration depending on the location. The amplified response in the structure showed a different variability depending on the type of structure and the target frequency.

• 대한물리치료과학회지
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• 제29권2호
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• pp.1-13
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• 2022

Background: This research was conducted to understand balance training in trunk control, balance, and walking in stroke patients. Design: Randomized controlled trial. Methods: The subjects included 40 stroke patients, of whom 20 undertook balance training using visual information and the other 20 undertook balance training using balance boards. Using visual feedback, the balance training group used a training program within the static balanced evaluation tool, while the balance training group trained using a balance board. All subjects underwent 20 mins of neurodevelopmental treatment, and both target groups underwent 10 mins each of balance training by using either visual feedback or a balance board. The treatment period lasted a total of 4 weeks, twice a day. Trunk control before and after training was evaluated with the Trunk Impairment Scale. Balance capability was assessed by the Berg Balance Scale, Functional Reach Test, Timed Up and Go test, and Static balance measurement tool. Walking capacity was measured using gait measuring equipment, and cadence and velocity were measured. Results: Both groups showed a significant improvement in their interstitial control, balance, and gait ability after the experiments compared to before the experiments (p<0.05). The difference between the two groups was not significant. The visual feedback balance training group showed a more substantial improvement than the balance board training group. Conclusion: In this study, we found that the balance training combined with visual feedback contributes to improving trunk control, balance, and gait in patients with hemiplegia due to stroke. In addition to this, I believe that balanced training combined with visual feedback can be used as a training method when considering patients who lack interstitial control, balance, and gait ability.

• 한국환경생태학회지
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• 제36권5호
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• pp.468-480
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• 2022

낙동강하굿둑의 건설로 원활한 용수 공급이 이루어졌으나, 하구 수생태계의 종적 연결성을 단절시켰다. 이에 낙동강하굿둑 개방에 대한 사회적 요구가 지속적으로 제기되어 왔으며, 2017년부터 낙동강하구역 기수생태계 복원을 위한 노력이 본격화되었다. 낙동강 하구에는 다양한 어종이 서식해 왔으며, 이들의 서식·이동 특성은 상이하므로 이를 고려한 수문운영은 기수생태계 복원에 필수적이다. 이에 본 연구에서는 낙동강하구에 서식·이동하는 어류를 모니터링을 통해 확인하였으며, 방류 시 수문운영 형태별, 개도별 평균 유속을 산출하여, 어종에 따른 원활한 소상 가능성을 분석하였다. 또한 대상어종을 선정하고, 주 분포 수심에 따른 수문운영 형태를 제시하는 등 낙동강 하구역의 기수생태계 복원을 적극적으로 수행하기 위한 수문운영 방안을 제시하였다.

• 로봇학회논문지
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• 제16권3호
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• pp.250-259
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• 2021

Path planning is one of the important technologies for automated parking. It requires to plan a collision-free path considering the vehicle's kinematic constraints such as minimum turning radius or steering velocity. In a complex parking lot, Rapidly-exploring Random Tree^* (RRT^*) can be used for planning a parking path, and Reeds-Shepp or Hybrid Curvature can be applied as a tree-extension method to consider the vehicle's constraints. In this case, each of these methods may affect the computation time of planning the parking path, path-tracking error, and parking success rate. Therefore, in this study, we conduct comparative analysis of two tree-extension functions: Reeds-Shepp (RS) and Hybrid Curvature (HC), and show that HC is a more appropriate tree-extension function for parking path planning. The differences between the two functions are introduced, and their performances are compared by applying them with RRT^*. They are tested at various parking scenarios in simulation, and their advantages and disadvantages are discussed by computation time, cross-track error while tracking the path, parking success rate, and alignment error at the target parking spot. These results show that HC generates the parking path that an autonomous vehicle can track without collisions and HC allows the vehicle to park with lower alignment error than those of RS.

• Journal of Positioning, Navigation, and Timing
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• 제11권2호
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• pp.71-81
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• 2022

In order for electro-optical tracking system (EOTS) to have accurate target coordinate, accurate navigation results are required. If an integrated navigation system is configured using an inertial measurement unit (IMU) of EOTS and the vehicle's navigation results, navigation results with high rate can be obtained. Due to the time-delay of the navigation results of the vehicle in the EOTS and scale factor errors of the EOTS IMU in high-speed and high dynamic operation of the vehicle, it is much more difficult to have accurate navigation results. In this paper, an integrated navigation system of EOTS which compensates time-delay and scale factor error is proposed. The proposed integrated navigation system consists of vehicle's navigation system which provides time-delayed navigation results, an EOTS IMU, an inertial navigation system (INS), an augmented Kalman filter and integration Kalman filter. The augmented Kalman filter outputs navigation results, in which the time-delay of the vehicle's navigation results is compensated. The integration Kalman filter estimates position, velocity, attitude error of the EOTS INS and accelerometer bias, accelerometer scale factor error, gyro bias and gyro scale factor error from the difference between the output of the augmented Kalman filter and the navigation result of the EOTS INS. In order to check performance of the proposed integrated navigation system, simulations for output data of a measurement generator and land vehicle experiments were performed. The performance evaluation results show that the proposed integrated navigation system provides more accurate navigation results.

• 한국음향학회지
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• 제41권6호
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• pp.601-609
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• 2022

2007년 8월 동해 연안에서 연안용승 현상이 발생 되었고, 이에 따른 수직적인 수온과 염분 자료가 실시간 해양관측부이를 통해 획득되었다. 시계열 관측자료를 기반으로 연안용승 발생 전·중·후의 수직적인 음속구조를 산출하였고, 연안용승의 수평적인 규모와 실제 해저지형 등을 고려한 음향모델링을 통해 연안용승이 수중의 음파 전달과 탐지환경에 어떠한 영향을 미치는지 분석하였다. 포물선 방정식 모델을 이용한 저주파(500 Hz) 음파 전달손실과 심도별 표적 탐지거리를 비교·분석한 결과, 동해 연안에서 용승이 발생하면 그렇지 않은 일반적인 음탐환경에 비해 최대 약 10 dB의 탐지이득을 기대할 수 있는 것으로 분석되었다. 또한, 이번 연구를 통해 용승 전·후 약 2 ~ 3일의 짧은 기간 내에서도 음파전달 특성이 크게 달라질 수 있음을 확인하였다.