• Journal of Advanced Navigation Technology
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• v.14 no.1
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• pp.49-56
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• 2010

Severe energy constraints and the low power consumption require the significance of the energy efficient error control mechanisms in wireless sensor network (WSN). In this paper, an automatic repeat request (ARQ) methodology for the analysis of error control schemes in WSN is presented such that the effects of packet length, the modulation scheme and the interference effect of the wireless channel are investigated. Moreover, an analyis of ARQ error control is provided by considering two major architectures for wireless sensor network, i.e., Mica2 and MicaZ sensor nodes. And the throughput performance of WSN with asynchronous FSK signal and DSSS-OQPSK signal with selective repeat ARQ scheme are analyzed in multiple interference environment, and the probability of receiving a correct bit and packet from target node to sink node is evaluated as a function of the channel parameter, the number of wireless sensor node, and the spreading factor.

• Journal of KIISE:Information Networking
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• v.34 no.1
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• pp.27-32
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• 2007

Next-generation wireless networks are expected to support a wide range of services, including high-rate data applications, Various service types request differentiated QoSs(Qualities of Service) such as minimum data rate, accuracy, fairness and so on. Although resources of radio systems are limited, for many applications, it is important that certain QoS targets are required to be met. In this paper, we propose a QoS based scheduling algorithm for next generation systems, based on analyzing previous researches, and we develop the proposed QoS algorithm only for MIMO(multi-Input Multi-Output) systems. Moreover, we subsequently prove that the proposed algorithm optimize throughput relative to prespecified target values and converge to certain throughput.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.24-30
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• 2008

Colletotrichum gloeosporioides forms an appressorium, a specialized infection structure, to infect its hosts. Among 400 and 600 culture filtrates from fungi and class Actinomycetes, six methanol extracts (A5005, A5314, A5387, A5560, A5597, and A5598) from the class Actinomycetes significantly inhibited appressorium formation in C. gloeosporioides infecting pepper fruits in a dose-dependent manner, while conidial germination was slightly enhanced. Two (A5005 and A5560) of them also exhibited distinctive inhibitory effect on the disease progress of pepper anthracnose. Water fractions of both culture filtrates also specifically inhibited appressorium formation in C. gloeosporioides and pepper anthracnose disease. Inhibition of appressorium formation by culture filtrate of A5005 was partially restored by the exogenous calcium. This results suggests that chemicals within A5005 extents its biological activity through disturbance of intracellular $Ca^{2+}$ regulation during prepenetration morphogenesis by C. gloeosporioides. Together, cell-based and target-oriented screening system used in this study should be applicable for other plant pathogenic fungi prerequisite appressorium formation to infect their hosts.

• Journal of the Optical Society of Korea
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• v.17 no.6
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• pp.513-517
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• 2013

Recently, maskless lithography (ML) systems have become popular in digital manufacturing technologies. To achieve high-throughput manufacturing processes, digital micromirror devices (DMD) in ML systems must be driven to their operational limits, often in harsh conditions. We propose an instrument and algorithm to detect DMD malfunctions to ensure perfect mask image transfer to the photoresist in ML systems. DMD malfunctions are caused by either bad DMD pixels or data transfer errors. We detect bad DMD pixels with $20{\times}20$ pixel by white and black image tests. To analyze data transfer errors at high frame rates, we monitor changes in the frame rate of a target DMD pixel driven by the input data with a set frame rate of up to 28000 frames per second (fps). For our data transfer error detection method, we verified that there are no data transfer errors in the test by confirming the agreement between the input frame rate and the output frame rate within the measurement accuracy of 1 fps.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.2
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• pp.33-40
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• 2008

Streaming video has become a popular form of transferring media over the wired/wireless network. TCP-friendly rate control (TFRC) is used as a streaming media transport protocol. Using the TCP congestion response function and current network conditions, TFRC adjusts its sending rate to yield the maximum TCP-friendly throughput. Since TFRC was designed for applications that would prefer to maintain a slowly-changing sending rate, it is less responsive to changes in handover between wireless heterogeneous networks such as 3G network, WLAN, and so on. This paper shows a new TFRC method for vertical handover over wireless heterogeneous network. The proposed TFRC method has features of low quality interruption and fast rate adaptation to a new target network. The simulation results show that the proposed one provides better QoS and throughput support than the traditional TFRC scheme during vertical handover.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.14-14
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• 2017

The discipline of plant breeding is experiencing a renaissance impacting crop improvement as a result of new technologies, however fundamental questions remain for predicting the phenotype and how the environment and genetics shape it. Inexpensive DNA sequencing, genotyping, new statistical methods, high throughput phenotyping and gene-editing are revolutionizing breeding methods and strategies for improving both quantitative and qualitative traits. Genomic selection (GS) models use genome-wide markers to predict performance for both phenotyped and non-phenotyped individuals. Aerial and ground imaging systems generate data on correlated traits such as canopy temperature and normalized difference vegetative index that can be combined with genotypes in multivariate models to further increase prediction accuracy and reduce the cost of advanced trials with limited replication in time and space. Design of a GS training population is crucial to the accuracy of prediction models and can be affected by many factors including population structure and composition. Prediction models can incorporate performance over multiple environments and assess GxE effects to identify a highly predictive subset of environments. We have developed a methodology for analyzing unbalanced datasets using genome-wide marker effects to group environments and identify outlier environments. Environmental covariates can be identified using a crop model and used in a GS model to predict GxE in unobserved environments and to predict performance in climate change scenarios. These new tools and knowledge challenge the plant breeder to ask the right questions and choose the tools that are appropriate for their crop and target traits. Contemporary plant breeding requires teams of people with expertise in genetics, phenotyping and statistics to improve efficiency and increase prediction accuracy in terms of genotypes, experimental design and environment sampling.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.8
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• pp.1770-1785
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• 2013

In this paper, a multi-hop transmission protocol based on parallel communication of secondary users (SUs) is proposed. The primary multi-hop network coexists with a set of SUs by cooperative spectrum sharing. The main optimization target of our protocol is the overall performance of the secondary system with the guarantee of the primary outage performance. The energy consumption of the primary system is reduced by the cooperation of SUs. The aim of the primary source is to communicate with the primary destination via a number of primary relays. SUs may serve as extra decode-and-forward relays for the primary network. When an SU acts as a relay for a primary user (PU), some other SUs that satisfy the condition for parallel communication are selected to simultaneously access the primary spectrum for secondary transmissions. For the proposed protocol, two opportunistic routing strategies are proposed, and a search algorithm to select the SUs for parallel communication is described. The throughput of the SUs and the PU is illustrated. Numerical results demonstrate that the average throughput of the SUs is greatly improved, and the end-to-end throughput of the PU is slightly increased in the proposed protocol when there are more than seven SUs.

• Molecules and Cells
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• v.23 no.3
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• Biomolecules & Therapeutics
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• v.22 no.5
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• pp.371-383
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• 2014

The nematode Caenorhabditis elegans (C. elegans) offers a unique opportunity for biological and basic medical researches due to its genetic tractability and well-defined developmental lineage. It also provides an exceptional model for genetic, molecular, and cellular analysis of human disease-related genes. Recently, C. elegans has been used as an ideal model for the identification and functional analysis of drugs (or small-molecules) in vivo. In this review, we describe conserved oncogenic signaling pathways (Wnt, Notch, and Ras) and their potential roles in the development of cancer stem cells. During C. elegans germline development, these signaling pathways regulate multiple cellular processes such as germline stem cell niche specification, germline stem cell maintenance, and germ cell fate specification. Therefore, the aberrant regulations of these signaling pathways can cause either loss of germline stem cells or overproliferation of a specific cell type, resulting in sterility. This sterility phenotype allows us to identify drugs that can modulate the oncogenic signaling pathways directly or indirectly through a high-throughput screening. Current in vivo or in vitro screening methods are largely focused on the specific core signaling components. However, this phenotype-based screening will identify drugs that possibly target upstream or downstream of core signaling pathways as well as exclude toxic effects. Although phenotype-based drug screening is ideal, the identification of drug targets is a major challenge. We here introduce a new technique, called Drug Affinity Responsive Target Stability (DARTS). This innovative method is able to identify the target of the identified drug. Importantly, signaling pathways and their regulators in C. elegans are highly conserved in most vertebrates, including humans. Therefore, C. elegans will provide a great opportunity to identify therapeutic drugs and their targets, as well as to understand mechanisms underlying the formation of cancer.

• Animal Bioscience
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• v.36 no.9
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• pp.1336-1349
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• 2023

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC]=0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.