• BMB Reports
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• v.49 no.8
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• pp.431-436
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• 2016

Human papillomavirus (HPV) is the major cause of cervical cancer, a deadly threat to millions of females. The early oncogene product (E7) of the high-risk HPV16 is the primary agent associated with HPV-related cervical cancers. In order to understand how E7 contributes to the transforming activity, we investigated the structural features of the flexible N-terminal region (46 residues) of E7 by carrying out N-15 heteronuclear NMR experiments and replica exchange molecular dynamics simulations. Several NMR parameters as well as simulation ensemble structures indicate that this intrinsically disordered region of E7 contains two transient (10-20% populated) helical pre-structured motifs that overlap with important target binding moieties such as an E2F-mimic motif and a pRb-binding LXCXE segment. Presence of such target-binding motifs in HPV16 E7 provides a reasonable explanation for its promiscuous target-binding behavior associated with its transforming activity.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2009.01a
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• pp.290-293
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• 2009

In this paper, we propose a method to estimate pointing region in real-world from images of cameras. In general, arm-pointing gesture encodes a direction which extends from user's fingertip to target point. In the proposed work, we assume that the pointing ray can be approximated to a straight line which passes through user's face and fingertip. Therefore, the proposed method extracts two end points for the estimation of pointing direction; one from the user's face and another from the user's fingertip region. Then, the pointing direction and its target region are estimated based on the 2D-3D projective mapping between camera images and real-world scene. In order to demonstrate an application of the proposed method, we constructed an ICGS (interactive cinema guiding system) which employs two CCD cameras and a monitor. The accuracy and robustness of the proposed method are also verified on the experimental results of several real video sequences.

• Journal of Powder Materials
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• v.6 no.1
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• pp.69-74
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• 1999

The microstructure of the reagion of carters, created by Cu and W-Cu shaped charge jets, in a 1020 mild steel target has been intestiaged. The region ahead of the crater created by the Cu shaped charge jet, reveals dramatic grain refinement implying the occurrence of a dynamic recrystallization, while that of W-Cu one dose a martensitic transformation indicative of heating up to an austenitic region followed by rapid cooling.The impacting pressure calculated when the W-Cu shaped charge jet encounters the target is higher than that of the Cu one. The micro-hardness of the region ahead of the crater created by the W-Cu shaped charge jet is also higher than that of the Cu one. The microstructure of W-Cu slug remained in the inside of the craters depicts the occurrence of the remarkable elongation of W particles during the liner collaphse. From these results, the microstructural variation of the region ahead of the crater with Cu and W-Cu shaped charge jets is discussed in trems of the pressure dependency of the transformation region of ferrite and austenite phases.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.677-680
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• 2003

Perceptual landmarks are an effective solution for a mobile robot realizing steady and reliable long distance navigation. But the prerequisite is those landmarks must be detected and recognized robustly at a higher speed under various lighting conditions. This made image processing more complicated so that its speed and reliability can not be both satisfied at the same time. Color based target detection technique can separate target color regions from non-target color regions in an image with a faster speed, and better results were obtained only under good lighting conditions. Moreover, in the case that there are other things with a target color, we have to consider other target features to tell apart the target from them. Such thing always happens when we detect a target with its single character. On the other hand, we can generally search for only one target for each time so that we can not make use of landmarks efficiently, especially when we want to make more landmarks work together. In this paper, by making use of the moment invariants of each landmark, we can not only search specified target from separated color region but also find multi-target at the same time if necessary. This made the finite landmarks carry on more functions. Because moment invariants were easily used with some low level image processing techniques, such as color based target detection and gradient runs based target detection etc, and moment invariants are more reliable features of each target, the ratio of target detection were improved. Some necessary experiments were carried on to verify its robustness and efficiency of this method.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.942-945
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• 1999

In this work, we proposed Proper etching algorithm for ultra-large scale integrated circuit device and simulated etching process using the proposed algorithm in the case of ICP (inductive coupled plasma) 〔1〕source. Until now, many algorithms for etching process simulation have been proposed such as Cell remove algorithm, String algorithm and Ray algorithm. These algorithms have several drawbacks due to analytic function; these algorithms are not appropriate for sub 0.1 ${\mu}{\textrm}{m}$ device technologies which should deal with each ion. These algorithms could not present exactly straggle and interaction between Projectile ions and could not consider reflection effects due to interactions among next projectile ions, reflected ions and sputtering ions, simultaneously In order to apply ULSI process simulation, algorithm considering above mentioned interactions at the same time is needed. Proposed algorithm calculates interactions both in plasma source region and in target material region, and uses BCA (binary collision approximation4〕method when ion impact on target material surface. Proposed algorithm considers the interaction between source ions in sheath region (from Quartz region to substrate region). After the collision between target and ion, reflected ion collides next projectile ion or sputtered atoms. In ICP etching, because the main mechanism is sputtering, both SiO$_2$ and Si can be etched. Therefore, to obtain etching profiles, mask thickness and mask composition must be considered. Since we consider both SiO$_2$ etching and Si etching, it is possible to predict the thickness of SiO$_2$ for etching of ULSI.

• Journal of the Korean institute of surface engineering
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• v.38 no.5
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• pp.179-182
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• 2005

Indium Tin Oxide (ITO) thin films were deposited from various target densities ($98.7\%\~99.6\%$) using RF magnetron sputtering. Effect of the sputtering target densities on the structural, electrical and optical properties of deposited ITO thin films was investigated. The preferable (400) crystalline orientation peak was observed on the films deposited from > $99.0\%$ target density. Higher target density produced films with higher roughness but lower resistivity. All of the deposited films showed optical transmittance more than $85\%$ in the visible wavelength region. It is necessary to use the highest target density for sputtering deposition of ITO thin films.

• Journal of Information Display
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• v.5 no.1
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• pp.41-46
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• 2004

In this paper, a new stereo 3D moving-target tracking system using the MSE (mean square error) and BPEJTC (binary phase extraction joint transform correlator) algorithms is proposed. A moving target is extracted from the sequential input stereo image by applying a region-based MSE algorithm following which, the location coordinates of a moving target in each frame are obtained through correlation between the extracted target image and the input stereo image by using the BPEJTC algorithm. Through several experiments performed with 20 frames of the stereo image pair with $640{\times}480$ pixels, we confirmed that the proposed system is capable of tracking a moving target at a relatively low error ratio of 1.29 % on average at real time.

• Journal of the Korea Institute of Military Science and Technology
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• v.13 no.3
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• pp.445-452
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• 2010

Adequate segmentation of target objects from the background plays an important role for the performance of automatic target recognition(ATR) system. This paper presents a new segmentation algorithm using fuzzy thresholding to extract a target. The proposed algorithm consists of two steps. In the first step, the region of interest(ROI) including the target can be automatically selected by the proposed robust method based on the frame difference of each image sensor. In the second step, fuzzy thresholding with a proposed membership function is performed within the only ROI selected in the first step. The proposed membership function is based on the similarity of intensity and the adjacency of target area on each image. Experimental results applied to real CCD/IR images show a good performance and the proposed algorithm is expected to enhance the performance of ATR system using multi-sensors.

• Biomedical Science Letters
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• v.26 no.2
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• pp.114-119
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• 2020

Of the various types of mice used for genome editing, conditional knock-out (cKO) mice serve as an important model for studying the function of genes. cKO mice can be produced using loxP knock-in (KI) mice in which loxP sequences (34 bp) are inserted on both sides of a specific region in the target gene. These mice can be used as KO mice that do not express a gene at a desired time or under a desired condition by cross-breeding with various Cre Tg mice. Genome editing has been recently made easy by the use of third-generation gene scissors, the CRISPR-Cas9 system. However, very few laboratories can produce mice for genome editing. Here we present a more efficient method for producing loxP KI mice. This method involves the use of an HDR vector as the target vector and ssODN as the donor DNA in order to induce homologous recombination for producing loxP KI mice. On injecting 20 ng/µL of ssODN, it was observed that the target exon was deleted or loxP was inserted on only one side. However, on injecting 10 ng/µL of the target HDR vector, the insertion of loxP was observed on both sides of the target region. In the first PCR, seven mice were identified to be loxP KI mice. The accuracy of their gene sequences was confirmed through Sanger sequencing. It is expected that the loxP KI mice produced in this study will serve as an important tool for identifying the function of the target gene.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.48 no.5
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• pp.55-63
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• 2011

This paper describes new moving target detection technique combining two algorithms to detect targets and reject clutters in video frame images for surveillance system: One obtains the region of moving target using phase correlation method using $N{\times}M$ sub-block images in frequency domain. The other uses adaptive threshold using learning weight for extracting target candidates in subtracted image. The block region with moving target can be obtained using the characteristics that the highest value of phase correlation depends on the movement of largest image in block. This technique can be used in camera motion environment calculating and compensating camera movement using FFT phase correlation between input video frame images. The experimental results show that the proposed algorithm accurately detects target(s) with a low false alarm rate in variety environment using the receiver operating characteristics (ROC) curve.