• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• 대한가정학회지
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• 제43권11호
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• pp.59-71
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• 2005

The purpose of this study was to observe the makeup behavior and cosmetics preference based on the segmented age group and fashion appearance interested group. The target consumers were women aged in their 20's. We distributed 443 questionnaires and conducted statistical analysis using SPSS program for the 400 reliable questionnaires. Statistical analyses included frequency, Chi-square test, t-test, ANOVA and cluster analysis(K-means). The results of this study were as follows: 1. There was a significant association between specific cosmetic items currently used and segmented age group. The age 24-29 had more basic makeup than color makeup, compared to the age 20-23 group. There was a statistical difference between the highly interested group and the lowly interested group in fashion and beauty. The group of highly interested in fashion and beauty considered trendy colors, while the group of lowly interested in fashion and beauty used their favorite color as an important factor in purchasing makeup products. 2. There was a significant difference in the preference of lip colors and textures, eye shadows textures, types of eye liner and types of foundation according to segmented age groups. 3. Those who sought 'sexy' images preferred red/wine/purple lipstick colors, and green/blue/violet eye shadow colors. They significantly preferred jell/liquid type eye liner and liquid type foundation. Those who sought 'pure' images preferred orange colors and glossy textures of lipsticks. Those who sought 'elegant' and 'intellectual' images preferred pearl type eye shadow.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제2권8호
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• pp.357-368
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• 2013

우리 사회는 광대역 ICT 인프라의 확충과 스마트 디바이스, 클라우드 서비스 및 소셜 미디어 서비스의 활성화로 인해 언제 어디서나 다양한 지식의 공유 / 관리 / 제어 / 창조가 가능한 혼합현실 환경의 상시 연결사회로 진화하고 있고 이로 인해 사이버 위협이 점차 증가하고 있는 상황이다. 향후 사회는 물리 및 논리공간의 융합, 스마트 객체의 유기적 연결, 상시연결 사회의 보편화로 인하여 사이버 위협에 대한 효과적 대응을 하지 못하는 경우에 더욱 복잡하고 미묘한 문제를 야기할 수 있어 APT와 같은 고도화된 사이버 위협에 대한 새로운 접근방법과 대응 체계에 관한 연구가 요구된다. 본 논문에서는 향후 다양한 미래서비스 환경 변화와 상시 연결사회의 보편화에 따른 새로운 유형의 사이버 위협에 능동적으로 대응하기 위한 기반으로써 국가 보안 Knowledge-Base 구축 전략을 제시한다.

• 대한수의학회지
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• 제42권1호
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• pp.55-64
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• 2002

HIV-1의 envelope glycoprotein은 중화항체에 의한 체액성 면역반응의 중요한 target으로 surface glycoprotein인 gp120과 transmembrane glycoprotein인 gp41로 이루어져 있다. gp120과 gp41의 ectodomain으로 이루어진 gp140 유전자를 PCR의 방법으로 증폭하고 Semliki Forest virus(SFV) 유래 expression system을 이용하여 mammalian 세포에서 발현하였다. 발현된 gp140은 natural HIV-1에서와 같이 oligomer를 형성하였다. 발현된 gp140을 정제하여 BALB/c 마우스에 접종하여 항체가 형성되었음을 확인하였다.

• Molecules and Cells
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• 제23권3호
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• 대한원격탐사학회지
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• 제35권5_1호
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• pp.727-736
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• 2019

본 연구에서는 여러 장의 무인항공기 영상을 사용하여 대상지역에 대한 포인트 클라우드를 생성하고, 데이터 세트 간 발생하는 이격과 중복점을 제거하는 방안에 대한 연구를 수행하였다. 이를 위해 사진 측량 기반의 IBA(Incremental Bundle Adjustment)기법을 적용하여 무인기의 위치/자세를 보정하고 스테레오 페어를 구성했다. 각각의 스테레오 영상으로부터 에피폴라 영상을 생성하고 MDR(Multi-Dimensional Relaxation) 정합 기법을 적용하여 포인트 클라우드를 생성하였다. 다음으로 스테레오 영상 간 관측 벡터에 기반한 포인트 클라우드 등록을 통해 서로 다른 스테레오 페어로부터 생성된 포인트 클라우드 간 이격을 제거하였다. 마지막으로 점유격자(Occupancy grid) 기반 통합 알고리즘을 적용하여 중복점이 제거된 하나의 포인트 클라우드를 생성하였다. 실험은 무인항공기에서 취득된 연직 촬영 영상을 사용하였으며, 실험을 통해 서로 다른 스테레오 페어로부터 생성된 포인트 클라우드 간 이격 및 중복점 제거가 가능함을 확인하였다.

• 패션비즈니스
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• 제22권5호
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• pp.64-82
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• 2018

As sustainability of fashion brands is becoming more important in the prolonged depression, the fashion market is also seeking ways to create new value through convergence with various fields. In the fashion market, convergence marketing is engaged with diverse technologies combined with advanced technologies and strategies. This study established the conceptual characteristics of convergence and divergence from a market perspective. Convergence can be summarized as Borderless, Compounding, Diversification, Recombination and Affiliation. The conceptual characteristics of the divergence can be summarized as de-fusion, individualization, differentiation, specialization, and segmentation. The characteristics of convergence revealed through the case of fashion market include the expandability of the new category, the expandability of the consumer, the brand experience, and the expandability of time and space. Divergence is the differentiation of brand value, target customer, and product nature. Through this study, we could understand the differentiation of the fashion market and the direction of the marketing strategy by looking at the characteristic of the convergence marketing - oriented research that has the contrary conceptual characteristics from the extended viewpoint. Based on this, it is possible to see the changes and patterns of brand marketing strategies and techniques in recent fashion market. In the future, it is expected that the systematic study of the marketing strategy of the domestic fashion brand based on the convergence and change characteristics of the fashion market proposed in this study will continue.

• 한국화재소방학회논문지
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• 제33권1호
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• pp.85-91
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• 2019

이 연구는 불꽃파장 감지기술과 불꽃영상 감지기술을 융합한 도로터널용 자동소화설비의 화재감지성능을 평가하기 위한 실험이다. 화재감지성능을 향상시키기 위한 이 융합기술은 화재 시 화원의 위치를 파악하고, 노즐을 화원으로 향하여 화재가 발생한 장소에만 가압수를 방사함으로서 화재진압에 따른 수손피해를 줄이는 효과를 얻을 수 있었다. 도로터널의 화재 중 불꽃 및 연기가 선행되는 상황에서 각각 15 m, 20 m, 25 m, 30 m, 35 m 거리에 $70cm{\times}70cm$의 목표물을 두었을 때 화원의 위치를 파악하는지를 실험하였다. 실험결과 농연의 간섭으로 인해 자외선 및 삼파장적외선센서의 감지능력이 감쇠되는 결과를 확인하였다. 또한 농연으로 인해 불꽃이 가려진 경우 이미지센서가 농연을 감지하여 화재신호를 발신함을 확인하였다.

• Biomolecules & Therapeutics
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• 제29권1호
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• pp.73-82
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• 2021

Hsp90 is often overexpressed with activated form in cancer cells, and many key cellular proteins are dependent upon the Hsp90 machinery (these proteins are called "client protein"). Nowadays, more client proteins and more inhibitors of Hsp90 are being discovered. Chaetocin has been identified as an inhibitor of histone methyl transferase SUV39H1. Herein, we find that Chaetocin is an inhibitor of Hsp90 which binds to the C-terminal of Hsp90α. Chaetocin inhibited a variety of Hsp90 client proteins including AMl1-ETO and BCL-ABL, the mutant fusion-protein in the K562 and HL-60 cells. SUV39H1 mediates epigenetic events in the pathophysiology of hematopoietic disorders. We found that inhibition of Hsp90 by Chaetocin and 17-AAG had ability to induce degradation of SUV39H1 through proteasome pathway. In addition, SUV39H1 interacted with Hsp90 through co-chaperone HOP. These results suggest that SUV39H1 belongs to a client protein of Hsp90. Moreover, Chaetocin was able to induce cell differentiation in the two cells in the concentration range of Hsp90 inhibition. Altogether, our results demonstrate that SUV39H1 is a new client protein of Hsp90 degradated by Chaetocin as a novel C-terminal inhibitor of Hsp90. The study establishes a new relationship of Chaetocin and SUV39H1, and paves an avenue for exploring a new strategy to target SUV39H1 by inhibition of Hsp90 in leukemia.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.816-823
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• 2022

The rapid spread of superbugs leads to the escalation of infectious diseases, which threatens public health. Endolysins derived from bacteriophages are spotlighted as promising alternative antibiotics against multi-drug resistant bacteria. In this study, we isolated and characterized the novel Salmonella typhimurium phage PBST08. Bioinformatics analysis of the PBST08 genome revealed putative endolysin ST01 with a lysozyme-like domain. Since the lytic activity of the purified ST01 was minor, probably owing to the outer membrane, which blocks accessibility to peptidoglycan, antimicrobial peptide cecropin A (CecA) was fused to the N-terminus of ST01 to disrupt the outer membrane. The resulting CecA::ST01 has been shown to have increased bactericidal activity against gram-negative pathogens including Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, and Enterobacter cloacae and the most affected target was A. baumannii. In the presence of 0.25 µM CecA::ST01, A. baumannii ATCC 17978 strain was completely killed and CCARM 12026 strain was wiped out by 0.5 µM CecA::ST01, which is a clinical isolate of A. baumannii and resistant to multiple drugs including carbapenem. Moreover, the larvae of Galleria mellonella could be rescued up to 58% or 49% by the administration of CecA::ST01 upon infection by A. baumannii 17978 or CCARM 12026 strain. Finally, the antibacterial activity of CecA::ST01 was verified using 31 strains of five gram-negative pathogens by evaluation of minimal inhibitory concentration. Thus, the results indicate that a fusion of antimicrobial peptide to endolysin can enhance antibacterial activity and the spectrum of endolysin where multi-drug resistant gram-negative pathogens can be efficiently controlled.