• KSBB Journal
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• v.24 no.6
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• pp.556-562
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• 2009

The biological treatment of TNT-containing wastewater, known commonly as pink water, was investigated using a stirred tank reactor with Stenotrophomonas maltophilia OK-5 bacterial culture. S. maltophilia OK-5 exhibited effective degradation of TNT contained in pink water, completely degrading TNT (100 mg/L) within 6 days of incubation. The dark-red brown color derived from Hydride-Meisenheimer complex became more pronounced during the incubation period, which was determined quantitatively. High-pressure liquid chromatography was used to measure residual TNT, which also resolved the metabolic intermediates (i.e., 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4-dinitro-6-hydroxytoluene). Gas chromatography-mass spectrometry was used to verify these intermediates. Quantification of the nitroreductase (pnrB) gene isolated from S. maltophilia OK-5 growing in pink water was performed with real-time PCR. The amount of pnrB gene copies increased to $10^3$-fold after 5 days of incubation time.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.161-164
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• 2010

Dollar spot, caused by Sclerotinia homoeocarpa F.T. Bennett, is the most economically important turfgrass disease on golf courses in North America due to its persistent nature. Fungicides, plant growth regulators, and other products were evaluated for preventative control of dollar spot and the secondary effects on brown patch and Pythium in a mixed stand of creeping bentgrass and annual bluegrass golf course putting green. Most fungicides provided excellent control of dollar spot throughout the trial but Rhapsody alone did not provide adequate control. Civitas applied alone and tank-mixed with Banner MAXX(R) and Daconil Ultrex(R) provided acceptable control throughout the study. No phytotoxicity was observed on any of the treatments applied but a darker green color and reduction in turfgrass growth was observed in a treatment with plant growth regulator, Trimmit. Treatments that contained Civitas and the Civitas Harmonizer also exhibited green pigmentation.

• Journal of IKEEE
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• v.24 no.1
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• pp.97-105
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• 2020

A thermal imaging sensor receives the radiating energy from the target and the background, so it has been widely used for detection, tracking, and classification of targets at night for military purpose. In recognizing the target automatically using thermal images, if the correct edges of object are used then it can generate the classification results with high accuracy. However since the thermal images have lower spatial resolution and more blurred edges than color images, the accuracy of the classification using thermal images can be decreased. In this paper, to overcome this problem, a new hierarchical classifier using both shape and local features based on the segmentation reliabilities, and the class/pose updating method for vehicle classification are proposed. The proposed classification method was validated using thermal video sequences of more than 20,000 images which include four types of military vehicles - main battle tank, armored personnel carrier, military truck, and estate car. The experiment results showed that the proposed method outperformed the state-of-the-arts methods in classification accuracy.

• Journal of Advanced Marine Engineering and Technology
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• v.39 no.4
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• pp.495-500
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• 2015

Currently the fish aquaculture industry of Korea is focused on the mass culture of flatfish (Paralichthys olivaceus) and and rockfish (Sebastes schlegeli) with completely controlled culture techniques. Recently, there has been considerable interest in new species development, such as the starry flounder (Platichthys stellatus). The value of starry flounder (Platichthys stellatus) as a raw fish increases with time because it is tasty, light, and bouncy. In this paper, the growth characteristics dependent on the LED wavelengths and the optimal growth conditions of the starry flounder were studied. In these experiments 4 different kinds of LED lighting, configurations were designed and prepared using red, green, blue and white, respectively. The fish aquaculture experiments were conducted over 10 weeks in four fish tanks, each installed with a different color of LED lighting. 10 starry flounders of 13 ~ 17g were placed into each tank. The effects of each color of light on the growth rate of the starry flounders were then examined. As a result, the starry flounders under the green LED lighting showed the highest growth rate, followed by the white, red, and blue LED lighting. Based on these results, a green light provides a suitable breeding environment for the starry flounder.

• Journal of the Korean Chemical Society
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• v.47 no.4
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• pp.363-369
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• 2003

Selenized yeast (Se yeast) containing $0.1{\%}$(w/w) of selenium was obtained when the yeast was incubated at a selenium concentration of 1$1.14{\times}10_-3 M$ in rich medium. After washing several times, the inorganic selenium on the cell wall was confirmed with MBRT. There was no indication of inorganic selenium on the cell wall when the blue color in MBRT was stayed for 15 minutes. The selenized yeast was sonicated, then the selenium contained protein was obtained after salting out by ammonium sulfate at the concentration $80{\%}$ saturation. The seven protein bands were seperated by SDS-PAGE and the selenium concentration in protein was measured by ICP-AES. Analytical data showed that the large expressed protein band contained a relatively large amount of selenium. The proteins of the 47kDa was contained the concentrations of 69.5 ${\mu}$ Se/g of most many content. The protein (47 kDa) was seperated from PVDF membrane by tank-electroblotting. The isolated protein was hydrolyzed under acid condition and reacted with PITC. The derivatives of amino acids were analyzed by HPLC and compared with the data obtained from regular yeast. The resulting selenium-yeast was analyzed with the selenomethionine concentration of $2{\%}$ comparaed with general amino acids. The goal of this study is to analyze the selenium concentration in protein bands and measure the degree of biotransformation of selenomethionine in a specific protein.

• Journal of Aquaculture
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• v.11 no.1
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• pp.49-58
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• 1998

The spawning behavior and early life history of the Korean aucha perch, Coreoperca herzi were studied. The eggs were spawned in a one-layer mass, hanging from the underside of a largy stone and guarded by one male. According to the considerable size difference os eggs in the individual ovaries, it was concluded that they spawns several times within a spawning season of May~June. The ripe eggs were demersal and adhesive, and their diameter were 2.60~3.04mm with several oil globule of 0.10~0.64mm. Hatching in the tank with $19.0{\pm}1.0^{\circ}C.$ in men water temperature started from the 380 hours after fertilization. Newly-hatched larvae were measured 6.45~7.34mm in total length (TL, mean : 6.85mm), and mouth and anus were open. Melanophores were present on the head, around the yolk, and on the dorsal part of the abdominal region. 3 days old larvae were measured 7.00~7.40mm in TL (mean : 7.25mm), and they fed Daphnia sp. and Brachionus plicatilis actively. 6 days old larvae transformed to postlarval stage and they were 7.94~10.10mm in TL (mean : 9.00mm). As yolk sac were nearly absorbed, the caudal notochord flex at 45. Aggregate numbers of all fin rays were completed at over 16.90~19.80mm in TL (30 days after hatching), at which time the larvae reached the juvenile stage. In fifty days old larvae(24.60~27.10mm in TL) were similar in body form, lateral line and color to adult.

• Applied Biological Chemistry
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• v.24 no.1
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• pp.59-66
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• 1981

Trichoxanthes Kirilowii with high starch-content was investigated to utilize as a food source. The weight and starch content of Trichoxanthes Kirilowii roots increased rapid1y in proporion to the growth period. The seasonal variations of the starch content were $36.5{\sim}48.0%$ (dry basis) from June to December. The chemical properties of natural growth soils of Trichoxanthes Kirilowii contained more organic matter and total nitrogen content than citrus orchard soils, but less mineral; P,K,Ca and Mg. To prepare starch with the plant root, the centrifugal method(3,000 rpm, 20 min) obtained the highest yield. The industrial-scale method, was similar to the processing of sweetpotato starch (tank precipitation method), and was good in quality. The starch granules were mainly spherical with diameters ranging from $5{\sim}16{\mu}$. The density of the starch was 1.535 and amylose content was about 26.7%. The X-ray diffraction pattern showed that the granules were of the B-type. The blue value of starch, amylose and amylopectin was 0.46, 0.80 and 0.18, respectively. The starch contained 0.05% ash, 0.35% crude protein and 34 mg% phosphorus. and had an inital pasting temperature of $63.5^{\circ}C$. The color intensity of the starch which iodine gave rise to absorption maxima at 670nm. The starch paste showed high stability during cold storage at $5^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.3
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• pp.191-201
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• 1987

Seasoned sardine meat was prepared to extend the use of sardine for human consumption, and processing conditions and storage stability of frozen seasoned sardine meat were studied during storage at $-20^{\circ}C$. The fish was beheaded, gutted and cleaned in a washing tank. The washed fish was then put through a belt-drum type meat separator which separates the flesh iron the bone and skin. Mechanically deboned fish meat was mixed with $20.6\%$ emulsion curd, $0.5\%$ table salt, $2.0\%$ sugar, $0.4\%$ sodium bicarbonate, $0.2\%$ polyphosphate, $0.1\%$ monosodium glutamate, $0.3\%$ onion powder, $0.1\%$ garlic powder, $0.1\%$ ginger powder, $3.0\%$ soybean protein and $0.1\%$. In sodium erythorbate. This seasoned sardine meat was frozen with contact freezer, packed in a carton box and then stored at $-20^{\circ}C$. The pH, volatile basic nitrogen, viable cell counts, peroxide value, carbonyl value, thiobarbituric acid value, taste compounds, fatty acid composition, salt extractable nitrogen, drip, texture, and color values of the products were determined during frozen storage. The results showed that lipid content in products could be controlled by using emulsion curd, and flavor and texture could be improved by adding spices and soybean protein, and lipid oxidation could be retarded by $0.1\%$ sodium erythorbate. Judging from the results of chemical experiments and sensory evaluation, the products can be preserved in a good quality for 120 days during frozen storage.