• 한국초지조사료학회지
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• 제18권2호
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• pp.133-142
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• 1998

This study was conducted to investigate the effects of antagonistic bacteria and pathogenic fungi on the growth of tall fescue(Festuca arundinacea Schreb.) in continuous cropping soil(CCS) and non-continuous cropping soil(NCCS). Tall fescue was established by seeding into pots of 11 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite, and cultivated at pots with antagonistic bacteria and pathogenic fungi in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified kern forage rhizosphere soil and fusants were isolated through cell hsion from B. subtilis and B. thwingiensis. B. subtilis was named as B. subtilis 101 and hsants were named as F-3, F-7 and F-8. In dark culture experiment, tall fescue inoculated with the antagonistic bacteria lived longer than that of control in both CCS and NCCS. However, tall fescue of CCS lived shorter than that of NCCS. Dry weight of tall fescue inoculated with the antagonistic bacteria was higher than that of tall fescue inoculated with pathogenic hngi in both CCS and NCCS(P< 0.05), and the antagonistic bacteria showed positive effects on the growth of tall fescue. However, Dry weight of tall fescue was decreased by the inoculation of the pathogenic b g i in both CCS and NCCS(P< 0.05).

• 한국초지조사료학회지
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• 제16권1호
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• pp.9-16
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• 1996

'This experiment was canied out to investigate the effects of endophyte status(end0phyte-free and -infect) and cutting frequency(3, 4, 5 and 6 timeslyear) on the agronomic characteristics, DM yield and weed development of tall fescue(Festuca arundinacea Schreb.) at the Experimental Field of Grassland and Forage Crops Division, National Livestock Research Institute, Suweon, from 1993 to 1994. 'Ihe results obtained are summarized as follows: DM yield of endophyte-infected tall fescue was slightly higher than that of endophyte-free tall fescue. DM yield of two tall fescue varieties was significantly decreased with frequent culting(p<0.05). Weed development of pasture tended to be decreased with endophyte-infected tall fescue and infrequent cutting, and endophyte-infected tall fescue had a good adaptation to the poor environment. Based on the results of this experiment, it is suggested that a slight increase in forage yield could be obtained from endophyte-infected tall fescue and endophyte-infected tall fescue was more persistant than endophyte-free tall fescue under poor conditions.

• 한국초지조사료학회지
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• 제21권2호
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• pp.45-48
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• 2001

In an effort to optimize tissue culture responses of tall fescue, the effects of basic medium and carbon sources on seed culturability and genotypic difference of plant regenerability were investigated. The MS basal medium was superior to $N_{5}$ 6/ and $B_{medium}$ in enhancing callus growth and plant regeneration. To determine the effect of carbon sources on plant regeneration, the seeds were cultured on medium with 30 g/L sucrose and maltose, respectively. Medium supplemented with 30 g/L sucrose resulted in regeneration of shoots from 50% of the calli. The genotypic difference in plant regenerability was obvious among five cultivars of tall fescue tested. \\`KY31\\` and \\`Hokuryo\\` showed to have higher regenerability with the frequency of 33% and 51%, respectively.

• 한국초지조사료학회지
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• 제16권1호
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• pp.17-26
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• 1996

This experiment was carried out to investigate the effects of endophyte staWendophyte-free and -infect) and cutting frequency(3, 4, 5 and 6 timeslyear) on the forage quality of tall fescue(Festuca crnrtzdit~crcerr Schreb.) at the Experimental Field of Grassland and Forage Crops Division. National Livestock Research Institute, Suweon, from 1993 to 1994. 'Ihe results obtained are summarized as follows: CP content of tall fexue was increased with endophyte-free and cutting frequency for two varieties. Crude protein yield(CPY) of tall fescue in 1993 was increased with endophyte-free and cutting frequency for two varieties(P<0.05). In 1994, however. CPY of endophyte-free tall fescue was slightly lower than that of endophyte-infected tall fescue and the higher CPY was observed when tall fescue was harvested 6 times per year. NDF content of tall fescue was not different between endophyte-infected and -free. NDF was decreased with cutting frequency for two varieties in 1993, however. it was not influenced by the endophyte and cutting frequency in 1994. ADF content of tall fescue was not different between endophyte-infected and -free. and it was decreased with cutting frequency for two varieties. IVDMD was not influenced by endophyte, however, it was increased with cutting frequency for two varieties. Based on the results of this experiment, it is suggested that a slight increase in forage quality could be obtained from endophyte-free tall fescue and the quality was increased with frequent cutting.

• 한국초지조사료학회지
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• 제18권3호
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• pp.195-204
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• 1998

This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

• 한국초지조사료학회지
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• 제17권4호
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• pp.363-370
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• 1997

This experiment was carried out to investigate the effect of the mixed rates of endophyte-he and -infected seed on the endophyte fkquency, dry matter yield and forage quality of tall fescue(Festuca arundinacea Schreb.) at the Experimental Field of Grassland and Forage Crops Division, National Livestock Research Institute, Suweon, from 1993 to 1994. The results obtained are summarized as follows: Endophyte frequency was increased with mixed rates of higher endophyte-infected seed, however, it was not different between mixed rates of endophyte-free and -infected seed. DM yield of tall fescue was not different between mixed rates of endophyte-fke and -infected seed, and CP, NDF, ADF and IVDMD contents of forage quality were not influenced by mixed rates of endophyte-he and -infected seed. However, the weed contents were slightly increased with high level of endophyte-free seed. The results demonstrated that endophyk-fke tall fescue did not seem to be greatly weak under poor conditions, if the weed contents were slightly increase.

• 한국축산시설환경학회지
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• 제20권4호
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• pp.189-194
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• 2014

A new tall fescue variety (Festuca arundinacea Schreb.) named Greenmaster and Purumi were. In this study, we evaluated the seed production of new tall fescue domestic varieties were cultivated in Korea. Agronomic aspects of tall fescue domestic varieties were determined using seed production and forage nutritive value of straw at the National Institute of Animal Science from 2013 to 2014. The heading date according to the varieties displayed differences and domestic varieties showed enhanced disease resistance. The harvested seed yields of Greenmaster was 35% higher as 1,664 kg/ha and it's also yield of straw higher than that of Fawn. However, the nutritive value of both varieties was similar. The dry matter yield of Pumumi was 25% higher as 11,080 kg/ha. Accordingly, developed a new variety of domestic tall fescue with excellent environmental adaptability, aiming to make contribution to the vitalization of the Korean grassland industry.

• 한국초지조사료학회지
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• 제7권2호
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• pp.109-112
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• 1987

tall fescue의 밀도변화에 미치는 토양경화는 공극율의 영향에 대하여 조사하였다. 1. 토양경화에 따라서 토양경도와 용적중은 증가되었다. (Table 1). 2. 10kg 처리구(토양경도 2.5kg/$\textrm{cm}^3$)의 밀도가 타처리구보다 유의하게 높았다. (Table 2, Fig. 1). 3. 토양공성율과 밀도와는 상관계수가 0.9804로써 1%수준의 정상란이 인정되었다. 4. 경화된 토양에서는 공성율이 37.5% 이상일 때 높은 밀도를 나타내었다.

• 아시안잔디학회지
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• 제15권4호
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• pp.169-179
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• 2001

근권부의 온도를 대조구에 비하여 낮게 조절하고 토양조성을 달리하여 여름철 고온기에 Kentucky bluegrass (Poa pratensis L.‘Nuglade’), per-ennial ryegrass(Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb. ‘Pixie’), Japanese lawngrass (Zoysia japonica Steud.)를 재배하고 잔디의 품질, 병해발생 정도 엽록소 함량을 조사하고 아울러 예지물의 무기물 함량을 측정하여 다음과 같은 결과를 얻었다. 1. 여름철 근권부의 온도를 4~6$^{\circ}C$ 낮게 처리한 경우 한지형 잔디의 균일성을 현저히 높여주고 병의 발생률을 크게 억제시켰으며 그 효과는 perennial ryegrass에서 가장 큰 것으로 나타났다. 엽록소 함량은 토양조성에 따라 변화가 없었으나 냉온 처리구에서 대조구에 비하여 크게 증가되었다. 2. 여름철 근권부의 냉온처리는 Kentucky blue-grass와 perennial ryegrass는 대조구에 비하여 약 2배, tall fescue는 2.5배 이상의 clipping무게 증가를 나타냈다. 난지형 잔디인 들잔디에서도 냉온처리구에서 생육의 증진효과를 보였다. 한지형 잔디는 모래 ＋peat moss를 80 : 20(v/v)으로 혼합한 토양에 비하여 모래＋peat moss＋일반토양을 80：10 : 10 (v/v/v)으로 혼합한 토양에서 많은 생장을 보였다. 3. 근권부의 냉온처리는 공시된 세 종류의 한지형 잔디에서 N, P, K, Ca그리고 Mg의 흡수를 증가시켰으나 들잔디에서는 흡수차이를 보이지 않았다. 토양의 혼합비율에 따른 무기물함량의 차이는 인정되지 않았다. 이상의 결과로 미루어 근권부의 냉온처리는 고온 스트레스에 의한 잔디의 생육저하를 감소시키고 여름철 하고현상을 예방하여 줌으로써 고온다습한 기후조건에서도 경기장의 잔디 품질을 우수하게 유지할 수 있는 방안으로 판단된다.

• Journal of Plant Biotechnology
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• 제32권3호
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• pp.187-193
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• 2005

톨 페스큐의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스는 6 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS배지에서 가장 높은 빈도로 유도되었으며, 식물체 재분화율도 증가시키는 것으로 나타났다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 N6배지에서 배양했을 때 50% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 품종간 배발생 캘러스의 형성율과 식물체 재분화율은 Kentucky-31 품종이 각각 58.3%와 52%로서 가장 높은 효율을 나타내어 품종 간에 큰 차이를 나타내었다. 탄소원으로는 sucrose를 첨가해주었을 때 식물체 재분화율이 55%로 증가되었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 톨 페스큐의 개발에 유용하게 응용되어질 수 있을 것이다.