• Korean Journal of Ichthyology
• /
• v.19 no.3
• /
• pp.185-192
• /
• 2007

The present experiment was carried out to study the effects of light intensity on growth, survival, caudal fin shape, body composition, skin color and blood component of river puffer, Takifugu obscurus. Four light intensities (0, 500, 1,000, 2,000 lx) were tested in duplicates for 60 days. Specific growth rate (SGR), daily feeding rate (FR) and food coefficient (FC) were reduced following to the decrease of light intensity. However in survival rate there was not significantly different among groups. The damage extent of caudal fin was increased in more strong light intensity. In body composition, moisture and crude protein were only significantly difference according to light intensity. In body color, L and a were the highest in 0 lx. In blood analysis, the content of AST, ALT, GLU significantly increased in more powerful the intensity groups. It is concluded that the supplement of low light intensity (e.g. 0 lx) recommended for the commercial scale aquaculture of river puffer in land-based tank without growth and survival retardation.

• Fisheries and Aquatic Sciences
• /
• v.24 no.11
• /
• pp.360-369
• /
• 2021

Paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) are neurotoxins that display pharmacological activity that is similar to that of specific sodium channel blockers; they are the principle toxins involved in shellfish and puffer fish poisoning. In Korea, puffer fish is a very popular seafood, and several cases of accidental poisoning by TTX have been reported. Therefore, it is necessary to determine whether puffer fish poisoning incidents are caused by PSTs or by TTX. In this study, we used mouse bioassay (MBA) and liquid chromatograph-tandem mass spectrometry (LC-MS/MS) to determine the presence of PSTs and TTX in puffer fish from an area near Mireuk-do, Tong-Yeong on the southern coast of Korea from January through March, 2014. The toxicity of PSTs and TTX extracts prepared from three organs of each specimen was analyzed by MBA. Most of the extracts killed mice with typical signs of TTX and PSTs. The LC-MS/MS analysis of seven specimens of Takifugu pardalis and Takifugu niphobles, each divided into muscles, intestines, and liver, were examined for TTX. In T. pardalis, the TTX levels were within the range of 1.3-1.6 ㎍/g in the muscles, 18.8-49.8 ㎍/g in the intestines, and 23.3-96.8 ㎍/g in the liver. In T. niphobles, the TTX levels were within the range of 2.0-4.5 ㎍/g in the muscles, 23.9-71.5 ㎍/g in the intestines, and 28.1-114.8 ㎍/g in the liver. Additionally, the toxicity profile of the detected PSTs revealed that dcGTX3 was the major component in T. pardalis and T. niphobles. When PSTs were calculated as saxitoxin equivalents the levels were all less than 0.5 ㎍/g, which is below the permitted maximum standard of 0.8 ㎍/g. These findings indicate that the toxicity of T. pardalis and T. niphobles from the southern coast of Korea is due mainly to TTX and that PSTs do not exert an effect.

• Korean Journal of Ichthyology
• /
• v.35 no.2
• /
• pp.67-74
• /
• 2023

The river pufferfish (Takifugu obscurus) is a valuable species in aquaculture and genetic studies. Usage of fish anesthetics aids in the easier handling of fish during aquaculture. However, there are no studies on appropriate conditions required for effective anesthetization of pufferfish. This study aims to determine the optimal conditions (fish age, water temperature, anesthetic concentration) needed for the most common fish anesthesia, MS-222, to anesthetize T. obscurus. We tested three different water temperatures (20℃, 24℃, and 28℃), three different anesthetic concentrations (125 mg/L, 150 mg/L, and 175 mg/L), and two different fish ages (one- and two-year-old). Appropriate anesthetization conditions for T. obscurus ranged from 150 mg/L to 175 mg/L of MS-222 at 24℃ to 28℃ for one-year-old fish. For two-year-old fish, the appropriate conditions ranged from 150 mg/L to 175 mg/L of MS-222 at 28℃. However, to minimize side effects and risks, 150 mg/L of MS-222 at 24℃ for one-year-old fish and 175 mg/L of MS-222 at 28℃ for two-year-old fish are recommended for effective anesthetization.

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2005.05a
• /
• pp.174-174
• /
• 2005

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2004.05a
• /
• pp.284-285
• /
• 2004

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2004.05a
• /
• pp.441-442
• /
• 2004

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2004.05a
• /
• pp.246-247
• /
• 2004

• Journal of fish pathology
• /
• v.17 no.2
• /
• pp.99-103
• /
• 2004

White spot disease by Cryptocaryon irritans broke out among cultured river puffer (Takifugu obscurus) cultured in sea water in Oct. 2001 and May 2004. Many white spots were easily observed on the gills with the naked eyes. The parasites attached on the gills at the base of the gill lamellae. The trophonts penetrated the interlamellar epithelium. Epithelial cell proliferation in interlamellar spaces occurred. PAS- positive mucous cells were prominent in the proliferated epithelium.

• Development and Reproduction
• /
• v.23 no.3
• /
• pp.239-253
• /
• 2019

We investigated the physiological responses of the river puffer, Takifugu obscurus, the tiger puffer, T. rubripes, a hybrids of the two (river puffer${\times}$tiger puffer) and a hybrid triploids to acute changes of salinity from 30 psu to 0 psu and from 0 psu to 30 psu. The blood and plasma factors of each species were elevated for 48, 72, or 96 hrs, and thereafter decreased due to hyper-osmoregulation and hypo-osmoregulation. In hyper-osmoregulation and hypo-osmoregulation, the cortisol concentration of river puffer, hybrids, hybrid triploids and tiger puffer increased for 12 or 48 hrs, and decreased thereafter. Chloride cells in the gill filaments of each species increased with increasing salinity, and melano-macrophages in the kidney tissue of each species increased with decreasing salinity. In conclusion, the hematological and stress responses of the hybrids were between those of the river puffer and tiger puffer, and the hematological responses of the hybrid triploids were higher than those of the other groups. The stress response of the hybrids was more sensitive than that of the hybrid triploids. In all groups, the histological responses of kidney in hyper-osmoregulation were more sensitive than those in hypo-osmoregulation.

• Development and Reproduction
• /
• v.24 no.4
• /
• pp.327-335
• /
• 2020

Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.