• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.335-340
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• 2013

Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both $10^4$ CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.295-300
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• 1989

Screening of Pseudomonas strains that can be used as hosts for expression of crystal protein gene of Bacillus thuringiensis subsp. kurstaki HD-73 was carried out. From rhizosphere soil of 7 kinds or crops as fluorescent Pseudomonas strains were isolated. A hybrie plasmid, pKTC1, composed of the broad host range vector pKT230 and the crystal protein gene was constructed and used for transformation of the 35 Pseudomonas strains. As the result, the crystal protein gene could be introduced into 4 isolates. Several methods including bioassay and immunochemical detection indicated that the crystall protein gene was expressed in the Pseudomonus isolates.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.193-198
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• 2012

This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (~35 kDa), dimer (~70 kDa), trimer (~105 kDa) and hexamer (~210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (~300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher's study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (${\beta}$-MeOH), anionic detergent (SDS) and heat ($95^{\circ}C$) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.

• Journal of Advanced Navigation Technology
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• v.18 no.6
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• pp.633-639
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• 2014

Recently the study about a smart health care which is combined IT with BT to provide a variety of health care services are being actively investigated. In order to provide the best possible emergency medical services in a short period of time, it is necessary that the rapid emergency measures in the event of an emergency essential. In this paper, we propose an emergency medical service platform to take effective first aid to person who has a NFC tag or NFC-enabled mobile smart phones in an accident. Using NFC, it is possible to help without physical contact to the patient unconscious to emergency incidents such as falling down in everyday life. In this paper, we design and implement an mobile emergency medical system that can deliver first aid information ask for help in case of emergency.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.46 no.3
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• pp.232-238
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• 2010

The moving ranges and behavior of four wild abalones, Haliotis discus hannai, were measured by an acoustic telemetry technique. The shape of the sea bottom of the experimental area was surveyed by a bathymetry system and three self-recording type acoustic receivers were used for monitoring the behavior and measuring the movement range. The abalones (WA1-WA4) attached acoustic tags were released and measured the movement during ten months. Three abalones (WA1, WA3 and WA4) were successively detected around the released point during the experiment and were moved to the V2 area where water depth is deeper than the V1 area. The change of inhabitation depth was also detected from the depth sensor of WA4. As the result, abalones were moved to deeper water area accordance with the decrease of the water temperature. The moved ranges of abalones were approximately 200 - 400m from the release point.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.783-788
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• 2020

Surges generated by direct strike or induction of lightning, frequent opening and closing of power system breakers, etc. cause excessive overvoltage, which can interfere with normal operation of transformers, circuit breakers, and power equipment. In order to prevent such phenomena, this study goes further from the method of establishing countermeasures by installing lightning arresters on power equipment or lines in parallel, and furthermore, remotely checking the condition of the lightning arrester and preparing immediate countermeasures when an event occurs. The purpose of the study is to prevent damage in advance.

• Journal of Life Science
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• v.28 no.1
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• pp.78-82
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• 2018

Detection of pathogenic microorganisms takes several days by conventional methods. It is necessary to assess microorganisms in a timely manner to reduce the risk of spreading infection. For this purpose, bacteriophages are chosen for use as a biosensing tool due to their host specificity, wide abundance, and safety. However, their lytic cycle limits their efficacy as biosensors. Phage proteins involved in binding to bacteria could be a robust alternative in resolving this drawback. Here, a fragment of tail protein J (residues 784 to 1,132) of phage lambda fused with 6X His-tag (6HN-J) at its N-terminus was cloned, overexpressed, purified, and characterized for its binding with microorganisms. The purified protein demonstrated a size of about 38 kDa in sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and bound with anti-His monoclonal antibodies. It bound specifically to Escherichia coli K-12, and not Salmonella typhimurium, Bacillus subtilis, or Pseudomonas aeruginosa in dot blotting. Binding of the protein to E. coli K-12 inhibited about 50% of the in vivo adsorption of the phage lambda to host cells at a concentration of $1{\mu}g/ml$ 6HN-J protein and almost 100% at $25{\mu}g/ml$ 6HN-J. The results suggest that a fusion viral protein could be utilized as a biosensing element (e.g., protein chips) for detecting microorganisms in real time.

• Journal of KIISE:Information Networking
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• v.37 no.5
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• pp.396-402
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• 2010

The tracking of current location of residents is an essential requirement for context-aware service of smart houses. This paper presents a wireless sensor network system which could detect location transition such as entrance and exit to a room and also identify the user who passed the room, without duty of wearing any sort of tag. We designed new sensor node to solve the problem of short operation lifetime of previous work[1] which has two pyroelectric infrared (PIR) sensors and an ultrasonic sensor, as well as a 2.4 GHz radio frequency wireless transceiver. The proposed user identification method is to discriminate a person based on his/her height by using an ultrasonic sensor. The detection idea of entering/exiting behavior is based on order of triggering of two PIR sensors. The topology of the developed wireless sensor network system is simple star structure in which each sensor node is connected to one sink node directly. We evaluated the proposed sensing system with a set of experiments for three subjects in a model house. The experimental result shows that the averaged recognition rate of user identification is 81.3% for three persons. and perfect entering/exiting behavior detection performance.

• IEMEK Journal of Embedded Systems and Applications
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• v.10 no.4
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• pp.189-198
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• 2015

Most IoT-related approaches have tried to establish the relation by connecting the network between things. The proposed research will present how the pervasive interaction of eco-system formed by touching the objects between humans and things can be recognized on purpose. By collecting and sharing the detected patterns among all kinds of things, we can construct the environment which enables individualized interactions of different objects. To perform the aforementioned, we are going to utilize technical procedures such as event-driven signal processing, pattern matching for signal recognition, and hardware in the loop simulation. We will also aim to implement the prototype of sensor processor based on Arduino MCU, which can be integrated with system using Arduino-Matlab/Simulink hybrid-interoperation environment. In the experiment, we use piezo transducer to detect the vibration or vibrates the surface using acoustic wave, which has specific frequency spectrum and individualized signal shape in terms of time axis. The signal distortion in time and frequency domain is recorded into memory tracer within sensor processor to extract the meaningful pattern by comparing the stored with lookup table(LUT). In this paper, we will contribute the initial prototypes for the acoustic touch processor by using off-the-shelf MCU and the integrated framework based on Matlab/Simulink model to provide the individualization of the touch-sensing for the user on purpose.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.10
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• pp.947-952
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• 2006

It has been confirmed that the inner defect of insulator and the perfect diagnosis for aging are closely related to safe electric power transmission system and that the detection of accident and diagnosis technique turn out to be very important issues. But perfect diagnosis is difficult because discharge pattern is irregular. Thus, we investigated discharge pattern using the new distribution statistical models with cross-inked polyethylene(XLPE) specimens. Voltage was applied to power frequency by step method, and calibration of discharge was set to 50 pC. After the voltage was applied, it measured the discharge occurring during 10s. We investigated discharge pattern using the K-means analysis and Weibull function. We also investigated variation of centroid and shape parameter due to variation of voltage. As a result of analyzing K-means, it was confirmed that cluster including many object numbers was formed by the presence of void. And result of Weibull distribution, it was confirmed that shape parameter of discharge varied from 1.28 to 1.62 in no void specimens, and that shape parameter of discharge number varied from 1.28 to 1.62. In the void, shape parameter of discharge varied from 5.66 to 6.43, and shape parameter of discharge number varied from 5.05 to 5.08.