• BMB Reports
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• 제44권1호
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• pp.52-57
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• 2011

Termites play an important role in the degradation of dead plant materials and have acquired endogenous and symbiotic cellulose digestion capabilities. The feruloyl esterase enzyme (FAE) gene amplified from the metagenomic DNA of Coptotermes formosanus gut was cloned in the TA cloning vector and subcloned into a pET32a expression vector. The Ft3-7 gene has 84% sequence identity with Clostridium saccharolyticum and shows amino acid sequence identity with predicted xylanase/chitin deacetylase and endo-1,4-beta-xylanase. The sequence analysis reveals that probably Ft3-7 could be a new gene and that its molecular mass was 18.5 kDa. The activity of the recombinant enzyme (Ft3-7) produced in Escherichia coli (E.coli) was 21.4 U with substrate ethyl ferulate and its specific activity was 24.6 U/mg protein. The optimum pH and temperature for enzyme activity were 7.0 and $37^{\circ}C$, respectively. The substrate utilization preferences and sequence similarity of the Ft3-7 place it in the type-D sub-class of FAE.

• 한국광물학회지
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• 제9권2호
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• pp.102-112
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• 1996

The precious serpentine, referring to a rare and highly valuable gem variety of serpentine group minerals, is found to occur in serpentinite from Booyo Gren Jade Mine which is located in Oesan-myun, Booyo-gun of Chungchungnam-do. Geommological properties of the precious serpentine have been investigated by use of polarizing microscope, specific gravity balance, refractometer, hardness pencils, X-ray diffractometer, XRF, ICP-MS analyser, and infrared absorption spectroscope.The precious serpentine from Booyo is colored deep green with oily luster and semi-transparent. It is highly tough and Mohs's scale of hardness is measured to be 5-6. Specific gravity is determined to be 2.67, and a single refractive index ND=1.56 is observed by a spot method, using sodium light source. X-ray powder diffraction data is represented by the reflection lines at 7.40(100), 4.64(25), 3.68(68), 2.757(69), 2.530(49), 2.549(32), and 1.710(21${\AA}$), which compares very well with that of antigorite of serpentine group minerals. The major chemical compositions of the precious serpentine group minerals. The major chemical compositions of the precious serpentine are SiO2 42.49%, MgO 39.08%, Fe2O3 3.85%, and H2O 11.87%. Besides, trace elements such as Cr(2188), Ni(1110ppm), Co(58ppm), and Ta (108ppm) are relatively spectrum shows peaks at 3670, 1190, 1070, 980 and 610cm-1. Strong absorption at 3670cm-1 is due to OH stretching, and 1190, 1070 and 980cm-1 due to SiO stretching. The absorption 610cm-1 is formed by alteration of pre-existing ultramafic rock, namely peridotite, with an introduction of fluid with very little content of CO2, under 400$^{\circ}C$ environment. Magnetite inclusions, finely disseminated in the precious serpentine, may be a result of Fe precipitation, during serpentinization of olivine-bearing country rock.

• 한국재료학회지
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• 제22권5호
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• pp.243-248
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• 2012

ZrN nanoparticles were prepared by an exothermic reduction of $ZrCl_4$ with $NaN_3$ in the presence of NaCl flux in a nitrogen atmosphere. Using a solid-state combustion approach, we have demonstrated that the zirconium nitride nanoparticles synthesis process can be completed in only several minutes compared with a few hours for previous synthesis approaches. The chemistry of the combustion process is not complex and is based on a metathesis reaction between $ZrCl_4$ and $NaN_3$. Because of the low melting and boiling points of the raw materials it was possible to synthesize the ZrN phase at low combustion temperatures. It was shown that the combustion temperature and the size of the particles can be readily controlled by tuning the concentration of the NaCl flux. The results show that an increase in the NaCl concentration (from 2 to 13 M) results in a temperature decrease from 1280 to $750^{\circ}C$. ZrN nanoparticles have a high surface area (50-70 $m^2/g$), narrow pore size distribution, and nano-particle size between 10 and 30 nm. The activation energy, which can be extracted from the experimental combustion temperature data, is: E = 20 kcal/mol. The method reported here is self-sustaining, rapid, and can be scaled up for a large scale production of a transition metal nitride nanoparticle system (TiN, TaN, HfN, etc.) with suitable halide salts and alkali metal azide.

• 대한미생물학회지
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• 제18권1호
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• pp.53-58
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• 1983

Enterotoxigenic E. coli is one of causative agents of the infantile diarrhea and traveler's diarrhea. A modified infant mouse assay(IMA) was developed for the detection of heat stable enterotoxin (ST) of E. coli isolated from diarrheal and control infants and assay system was established with using enterotoxin producing reference strains. The supernatant of the 24 hour-shaking culture of E. coli in Casamino Acid Yeast Extract Salt Broth(CYES-2) was ingested orally into the 2-4 day old ICR mice. After the mice were kept at $25^{\circ}C$ for 4 hours, they were sacrificed and the gut weight body weight ratio(GW/BW) was taken as the index of fluid accumulation induced by heat stable enterotoxin of E. coli. The results obtained were as follows; 1. The GW/BW responses of IMA tested with enterotoxin reference strains of E. coli(E. coli O148H28:$ST^+LT^+$, E. coli $O78H^-:ST^+LT^+$, E. coli O15H11:$ST^-LT^+$, E. coli O1H7:$ST^-LT^-$) appeared ta be ST dose-dependent, and not LT-dependent. From the dose-response curve, $25{\mu}l$ of culture supernatant was determined as test amount of the IMA. 2. Frequency distribution of IMA result from 643 strain of E. coli showed normal distribution at low GW/BW ratio and dispersed pattern at high GW/BW ratio. The GW/BW ratios of $0.056{\pm}0.004(mean{\pm}SD)$ of normal distribution which distributed from 0.044 to 0.068(P<0.01) was considered as ST negative. Thus the GW/BW ratio above 0.069 could be regarded as ST positive.

• 생태와환경
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• 제42권4호
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• pp.413-421
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• 2009

Eleven lake sediment core samples were obtained and analyzed to develop a chronology using $^{137}Cs$ (in 1963) and two tephra layers (Ko-c2 in 1694 and Ta-a in 1739). Sedimentation rates estimated for the past ca 300 years in Lake Shirarutoro indicated that catchment development has influenced the shallowing process in the lake by increasing sediment production. The sediment yield under initial land-use development conditions for the first two periods was estimated as 514 tons $yr^{-1}$ from 1694 to 1739 and 542 tons $yr^{-1}$ from 1739~1963. The development of the Shirarutoro catchment intensified in the 1960s with deforestation and agriculture activity leading to an increased sediment yield of 1261 tons $yr^{-1}$ after 1963. The sediment yields after intensified land use development, such as forestry and agricultural development, were about 2 times higher than that under initial development conditions, leading to accelerated lake shallowing over the last ca 50 years. Sedimentation rates differed with location in the lake because of spatial variation in the sediment flux from the contributing rivers and their catchments. The sedimentation rates before 1963 were low in all sites except for one site close to the Shirarutoroetoro River. The sedimentation rate in 1739~1963 was accumulated mostly at the inflow of the Shirarutoroetoro River by sediment production associated with forestry for charcoal production and initial agricultural development. The sedimentation rate after 1963 increased. In particular, the southern zone of the lake near the conjunction with the Kushiro River had a high sedimentation rate, which is attributable to sediment inflow back from the Kushiro River during floods.

• Corrosion Science and Technology
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• 제17권4호
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• pp.154-165
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• 2018

During the process of sulfur dioxide removal, flue gas desulfurization equipment provides a serious internal corrosion environment in creating sulfuric acid dew point corrosion. Therefore, the utilities must use the excellent corrosion resistance of steel desulfurization facilities in the atmosphere. Until now, the trend in developing anti-sulfuric acid steels was essentially the addition of Cu, in order to improve the corrosion resistance. The experimental alloy used in this study is Fe-0.03C-1.0Mn-0.3Si-0.15Ni-0.31Cu alloys to which Ru, Zn and Ta were added. In order to investigate the effect of $H_2SO_4$ concentration and the alloying elements, chemical and electrochemical corrosion tests were performed. In a low concentration of $H_2SO_4$ solution, the major factor affecting the corrosion rate of low alloy steels was the exchange current density for $H^+/H_2$ reaction, while in a high concentration of $H_2SO_4$ solution, the major factors were the thin and dense passive film and resulting passivation behavior. The alloying elements reducing the exchange current density in low concentration of $H_2SO_4$, and the alloying elements decreasing the passive current density in high concentration of $H_2SO_4$, together play an important role in determining the corrosion rate of Cu-bearing low alloy steels in a wide range of $H_2SO_4$ solution.

• 한국작물학회지
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• 제10권
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• pp.79-83
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• 1971

뉴지랜드의 황갈색 고원 토양에 있어 Lucerne의 근류형성 및 생육에 미치는 근류접종, pelleting 및 석회시용의 효과를 시험코저 5개 수준의 종자 처리(블접종, 접종, 접종 석회 pelleting, 접종인산 백운석 50/50 pelleting, 접종활석 pelleting과 석회시용(25kg/10are)을 비교하고 그들의 상호작용을 관찰하였던바 다음과 같은 결과를 얻었다. 1. 산성토양에서 Lucerne의 효과적 근류 형성에는 석회시용과 종자의 근류균 접종 및 pelleting이 필요하다. 2. 접종 종자를 적당한 재료로 싸면 근류헝성을 좋게한다. 3. Lucerne의 종자 pelleting은 석회시용 요구량을 감소시키는 수단이 될 수 있다. 4. 창갈색 고원 토양에서는 석회분말이 좋은 pelleting재료가 된다. 5. 종자에 접종하지 않거나 pelleting을 하지 않은 종자의 파종시는 석회시용이 효과적이다. 6. pelleting 종자 사용은 석회시용에 소요되는 품과 경비를 절감시킬 것이다.

• 대한수의학회지
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• 제35권3호
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• pp.531-536
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• 1995

Salmonella species are the most prevalent etiologic agents of food-borne acute gastroenteritis. Direct isolation of bacteria from the contaminated food, stool and animal tissues has been used for the diagnosis of salmonellosis routinely. However, isolation of bacteria is time consuming work and not so highly sensitive. In recent years, improved methods of polymerization chain reaction(PCR) and probe hybridization technique have led to the developement of diagnostic assays which employ to detect various human and animal pathogenic bacteria. In this study, we have performed the polymerization chain reaction to detect Salmonella pullorum from tissues and stool samples of chickens with two specific primers, ST5 and ST8C. The target DNA fragment of PhoE gene was successfully amplified from liver, spleen, pancreas, heart, lung, ovary, oviduct and feces samples. The amplified DNA fragments were hybridized with Salmonella typhymurium TA3000 PhoE probe by Southern hybridization. The PCR to amplify the PhoE gene was highly rapid and sensitive method to detect Salmonella pullorum from tissues and stool samples.

• Preventive Nutrition and Food Science
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• 제1권1호
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• pp.23-29
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• 1996

This study was conducted to investigate the changes in chemical, microbial and sensory characteristics, and antimutagenicity of buchu(leek, Allium odorum) kimchi during fermentation at 15$^{\circ}C$. Reducing sugar contents and pH of buchu kimchi were decreased during the fermentation. The reduction rate of reducing sugar of glutinous rice paste and shrimp added buvhu kimchi(GSBK) was faster than those of control buchu kimchi(CBK) and glutious rice paste added bucku kimchi(GBK). Acidity increased rapidly until 4 days,a nd optimum acidity (0.6%) of bucku kimchi was reached within 2 day. Also total bacterial and lactic acid bacterial counts greatly increased after 4 days of the fermentation. The numbers of lactic acid bacteria after 8 day- fermentation in CBK and GSBK, and 10 day-fermentation in GBK were the highest values, 4.5$\times${TEX}$10^{8}${/TEX} CFU/ml, 4.8$\times${TEX}$10^{8}${/TEX} CFU/ml and 6.1$\times${TEX}$10^{8}${/TEX} CFU/ml, respectively. In the sensory evaluation, appearance of sample was good at 0 day, taste from overall quality of buchu kimchi were asquired the highest values at 6th day. The methanol extracts from buchu kimchi(GBK) showed antimutagenicity against aflatoxin {TEX}$B_{1}${/TEX}({TEX}$AFB_{1}${/TEX})in Salmonella typhimurium TA100. The inhibition ration were 58~69% with treatment of the 5% methanol extracts, and when the adding concentration increased the effect increased.

• 한국자기학회지
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• 제8권6호
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• pp.369-373
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• 1998

본 연구에서는 마그네트론 스퍼터링 법으로 제작한 Ni-Fe/NiO 이층 박에서, NiO 증착 중 Ar 압력에 따른 교환이 방성의 변화를 고찰하엿으며 이를 미세조작과 관련시켜 해석하고자 하였다. 낮은 Ar 압력에서 증착한 Ni-Fe/NiO 이층막은 우수한 교환이방성 특성을 나타내었으나 Ar 압력이 증가함에 따라 교환이방성은 급격하게 감소하였다. 낮은 Ar 압력에서 증착한 시편은 NiO와 Ni-Fe 계면에서 epitaxy 경향을 나타내었으며 그 계면은 평범하고 그 경계는 두렷하게 구분하였다. 그러나 높은 Ar 압력에서 증착한 시편은 NiO와 Ni-Fe의 경계가 뚜렷하게 구분되지 않고 그 계면 또한 평활하지 않았다. 한편 NiO의 조성은 Ar압력의 증가에 따라 산소의 조성이 점점 증가하였다.