• Journal of Veterinary Science
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• 제21권3호
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• pp.49.1-49.14
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• 2020

Background: Coinfection with avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) is common in chickens, and the molecular mechanism of the synergistic pathogenic effects of the coinfection is not clear. Exosomes have been identified as new players in the pathogenesis of retroviruses. The different functions of exosomes depend on their cargo components. Objectives: The aim of this study was to investigate the function of co-regulation differentially expressed proteins in exosomes on coinfection of ALV-J and REV. Methods: Here, viral replication in CEF cells infected with ALV-J, REV or both was detected by immunofluorescence microscopy. Then, we analyzed the exosomes isolated from supernatants of chicken embryo fibroblast (CEF) cells single infected and coinfected with ALV-J and REV by mass spectrometry. KEGG pathway enrichment analyzed the co-regulation differentially expressed proteins in exosomes. Next, we silenced and overexpressed tripartite motif containing 62 (TRIM62) to evaluate the effects of TRIM62 on viral replication and the expression levels of NCK-association proteins 1 (NCKAP1) and actin-related 2/3 complex subunit 5 (ARPC5) determined by quantitative reverse transcription polymerase chain reaction. Results: The results showed that coinfection of ALV-J and REV promoted the replication of each other. Thirty proteins, including TRIM62, NCK-association proteins 1 (NCKAP1, also known as Nap125), and Arp2/3-5, ARPC5, were identified. NCKAP1 and ARPC5 were involved in the actin cytoskeleton pathway. TRIM62 negatively regulated viral replication and that the inhibition of REV was more significant than that on ALV-J in CEF cells coinfected with TRIM62. In addition, TRIM62 decreased the expression of NCKAP1 and increased the expression of ARPC5 in coinfected CEF cells. Conclusions: Collectively, our results indicated that coinfection with ALV-J and REV competitively promoted each other's replication, the actin cytoskeleton played an important role in the coinfection mechanism, and TRIM62 regulated the actin cytoskeleton.

• 대한조선학회논문집
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• 제54권3호
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• pp.258-266
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• 2017

Numerical simulations of turbulent two phase flows around KCS have been performed to investigate effects of trim conditions on ship resistance of KCS in short waves by utilizing waves2foam. The wave lengths of LPP/2 and LPP/4 with 1m and 2m wave heights were imposed at inlet boundary. The resistance reduction at 2m trim by head and the increase of resistance at trims by stern were observed regardless of wave lengths and wave heights. The hull pressure on fore-and-aft rather than wave patterns around bulbous bows was mainly responsible for the total resistance coefficients of KCS in short waves. A phase diagram of contribution of hull pressure to the total resistance coefficients disclosed that the phase of representing the maximum resistance in time history played an important role in the effect of trim conditions on ship resistance of KCS in short waves.

• 한국소음진동공학회논문집
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• 제16권12호
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• pp.1252-1261
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• 2006

This paper summarizes theoretical work on the multichannel decentralized feedback control of sound radiation from aircraft trim panels using piezoceramic actuators. The aircraft trim panels are generally honeycomb structures designed to meet the design requirement of low weight and high stiffness. They are resiliently-mounted to the fuselage for the passive reduction of noise transmission. It is motivated by the localization of reduction in vibration of single channel active trim panels. 12-channel decentralized feedback control systems are investigated in terms of the reduction of noise and vibration for three configurations of sensor actuator pairs. Local coupling of the closely-spaced sensor and actuator pairs was modeled using single degree of freedom systems. The multichannel control system is characterized using the state-space model. For the stability point of view, the relative stability or robustness is evaluated by comparing the real part of eigenvalues of the system matrix for the three configurations. The control performance is also evaluated and compared for the three configurations. It is found that the multichannel system can lead to the globalization of the reduction in vibration and radiated noise. It does not appear to yield a significant improvement in the vibration because of decreased gain margin. However, the reduction in the radiated noise is remarkably improved due to the variation of the vibration pattern with the actuation configurations.

• 한국해양공학회지
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• 제29권6호
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• pp.411-417
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• 2015

The shipping and shipbuilding industries have had business difficulties since the implementation of regulations on the CO₂ emissions from ships by IMO and the occurrence of the global financial crisis in 2008. Under this global recession, most shipping firms have started to operate their fleets at slow steaming rates with the goal of improving the profit ratio per transported unit. This study analyzed the resistance performance of a 6,800 TEU container ship corresponding to its trim variation with slow steaming, compared with that at its original design speed. Two different grid systems were used for the numerical calculation, one that considered the free surface allowing the capture of the dynamic trim and one that did not. This made it possible to clearly classify each resistance component to provide useful information to hull-form designers. In addition, a form factor assumption method using CFD was used for a reasonable effective power prediction in compliance with the 1978 ITTC performance prediction method. It was found that the total resistance of a 6,800 TEU container ship was reduced by 2.6% in the case of a 1-m trim at the bow at 18 kn.

• 대한조선학회논문집
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• 제36권4호
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• pp.56-63
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• 1999

47.7m급 쌍동선의 1/21.5 축척 모형선으로 예인수조에서 저항 및 내항성능 시험을 수행하였다. 저항 시험은 선미트림탭을 부착하여 트림탭각의 변화에 따른 트림 및 저항변화를 조사하여 트림 탭이 없는 경우와 비교하고 그 영향을 조사하였다. 해상상태 3,4 및 5에서 3가지 실선속력에 대해서 상하 및 종운동을 계측하였으며 갑판위 5점에서 수직가속도도 계측하였다. 측정한 가속도 값은 ISO 2631/3의 규정과 비교하여 설계선형의 우수함을 보였다.

• 한국항공우주학회지
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• 제31권1호
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• pp.42-48
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• 2003

Lever II 헬리콥터 비행운동 방정식으로부터 주기성 트림해를 구하기 위해 DAE 해법에 근거한 PPTA(Partial Periodic Trimming Algorithm)를 제안하였다. PPTA의 반복계산으로 수정된 상태변수는 적합한 초기조건이 요구되는 DAE해법에서 수치불안정을 일으킬 수 있다. 간단하게 DAE 차수를 조절함으로써 정확한 주기성 트림을 얻을 수 있었다. 수치해법을 CBM(Common Baseline Model) 헬리콥터에 적용하여 harmonic balance 방법과 동일한 트림해를 얻었으며 시뮬레이션 초기의 과도응답을 효과적으로 제거할 수 있음을 밝혔다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2007년도 춘계학술대회 논문집
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• pp.589-590
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• 2007

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3074-3079
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• 2007

The conventional control valves have been used at the locations occurring high differential pressure and high temperature which causes cavitation, flashing, severe vibration due to abrupt flow change, and sudden pressure drop. Previous studies concerning control valves focused to prevent damage of valve trim due to the internal leak and low flow rate. The newly designed helical trim of control valve has been installed at the location of high pressure change and high temperature in a power plant, and operated for evaluation. It is confirmed that the new control valve developed in this study generates flow characteristics in comparison with previous helical trim of control valves.

• 한국유체기계학회 논문집
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• 제10권4호
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• pp.61-70
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• 2007

Numerical analysis of three dimensional incompressible turbulent flows in LNG marine high pressure drop control valves was carried out by using the CFD-ACE from ESI-Group. In this study, flow characteristics of control valves with complex flow fields including cavitation effect were investigated. Simulation was performed on five models of control valve that had different orifice diameters of anti-trim and the size of valve. Comparing newly designed control valves for controling the occurrence of cavitation with the conventional valve, new valves showed a improved flow pattern with almost no cavitation.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.148.3-148
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• 2001

This paper deals with a problem of decreasing the airspeed and the altitude of a transport aircraft using thrust only. Such a situation can occur, if the aircraft loses all hydraulic power that drives the control surfaces. A controller for flight path angle control is designed using the model following servo control method, which is a PI-type optimal regulator. For computer simulation, a simulation model that covers a range of flight envelope is made using given linear models and trim points at some flight conditions. Nondimensional aerodynamic coefficients, derivatives and trim points that are not at the given trim points are computed by linear interpolation. The model is effective in simulation where the trim point varies. Simulation using ...