• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.313-316
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• 2000

When 3 recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B-22-4, $CS13-1.00^{\ast}$ and $CSl3-0.02^{\ast}$, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. Glycine betaine was found to have a strong osmoprotective effect on all 3 rCHO cell lines. Inclusion of 15 mM glycine betaine in hyperosmolar medim enabled rCHO cell lines to grow at 557-573 mOsm/kg where they could not grow in the absence of glycine betaine. However, effect of glycine betaine inclusion in hyperomolar medium on foreign protein production differed among rCHO cell lines. CHO/dhfr-B22-4 cells retained enhanced specific human thrombopoietin (hTPO) productivity in the presence of glycine betaine, and thereby, the maximum hTPO titer obtained at 573 mOsm/kg was increased by 72% over that obtained in the control culture with physiological osmolality (292 mOsm/kg). On the other hand, enhanced specific antibody productivity of $CSl3-1.00^{\ast}$ and $CSl3-0.02^{\ast}$ at elevated osmolality decreased significantly in the presence of glycine betaine at a cost of the recovery of cell growth. As a result, the maximum antibody titer at 557 mOsm/kg was similar to that obtained in the control culture with physiological osmolality. Taken together, efficacy of the simultanous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein production was variable among different rCHO cell lines.

• Current Optics and Photonics
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• 제2권1호
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• pp.90-95
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• 2018

Stimulated polariton scattering from the $B_1$-symmetry modes of a $KNbO_3$ crystal to generate a terahertz wave (THz-wave) with a noncollinear phase-matching scheme is investigated. The frequency-tuning characteristics of the THz-wave by varying the phase-matching angle and pump wavelength are analyzed. The expression for the effective parametric gain length under the noncollinear phase-matching condition is deduced. Parametric gain and absorption characteristics of the THz-wave in $KNbO_3$ are theoretically simulated. The characteristics of $KNbO_3$ for a terahertz parametric oscillator (TPO) are compared to those of $MgO:LiNbO_3$. The analysis indicates that $KNbO_3$ is an excellent optical crystal for a TPO, to enhance the THz-wave output.

• 한국건축시공학회지
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• 제18권5호
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• pp.463-469
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• 2018

기존의 다층으로 구성되어 시공성이 낮은 옥상녹화 공법의 개선을 위해 개발한 방수 방근 시트 일체화 옥상녹화박스 유닛공법의 일체화를 위해 사용하는 전자기 유도가열방식의 금속 고정구와 콘 형상 고정구의 부착성능을 평가하기 위해 엔지니어링 PE, TPO, PVC 시트 등 3종류의 시트에 가열온도에 따른 부착성능 및 동일한 시험체로 냉열반복 후 부착성능을 측정하였다, 그 결과는 엔지니어링 PE시트에 부착한 고정구의 부착성능이 가장 우수하게 나타났으며, 금속고정구는 가열온도가 올라갈수록, 콘 형상 고정구는 가열온도가 낮을수록 우수한 부착성능을 보여주었고, 냉열 반복 후 부착시험 결과는 상온 부착시험 결과와 동일하게 나타났다. 기존의 양면 부틸테이프 고정방식의 콘 형상 고정구는 낮은 부착하중과 냉열반복에 의한 뚜렷한 성능저하가 발생하는데 비하여 전자기 유도가열방식의 고정구는 우수한 성능을 유지하였다.

• Korean Chemical Engineering Research
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• 제54권4호
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• pp.560-567
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• 2016

본 연구에서는 $Ni/Al_2O_3$ 촉매의 수증기 개질반응 및 표면 특성을 조사하였다. 조촉매로써 선정된 Mo를 담지하여 제조한 Ni-Mo계 촉매를 Ni계 촉매와 반응활성 비교결과 효율증진 인자를 확인할 수 있다. $H_2$-TPR 및 XPS 분석을 통하여 효율이 저하되는 특성을 확인하였다. 수증기 개질반응 long run 실험 후 촉매표면에 침적된 carbon의 침적특성 및 결합구조, 기화특성을 확인하기 위하여 $O_2$-TPO 분석을 수행하였다. 본 연구를 통하여 수증기 개질반응에서 Ni과 강한 상호작용으로 결합하여 촉매의 반응활성 저하를 일으키는 graphitic carbon 종 형성을 억제함으로 Ni-Mo계 촉매에서 내구성이 증진됨을 확인할 수 있다.

• 공업화학
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• 제5권2호
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• pp.295-304
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• 1994

담지촉매인 $Mo-V-O/SnO_2$(VM/Sn)와 $SnO_2/Mo-V-O$(Sn/VM) 촉매를 제조하여 XRD, BET, SEM, $NH_3$, TPD 등으로 특성분석을 실시하였다. 고정층 연속 반응기에서 아크롤레인의 산화반응을 수행한 결과 이들 촉매의 아크롤레인 전화율과 아크릴산 수율이 Mo-V-O 자체보다 높았다. 이와 같은 상승효과의 원인을 TPD, TPR, TPO 등의 방법으로 고찰한 결과 Mo-V-O와 $SnO_2$의 접촉면에서 Mo-V-O가 $SnO_2$ 상으로 전자를 전달하고 $SnO_2$는 spill-over oxygen을 Mo-V-O 상으로 전달함으로써 반응에 의하여 부분환원된 Mo-V-O의 재산화를 촉진시키는 상간협동 현상이 존재하기 때문으로 판단되었다.

• Korean Chemical Engineering Research
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• 제48권6호
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• pp.684-689
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• 2010

본 연구에서는 다양한 구조 유도제로 합성된 SAPO촉매를 이용하여 자일로오스의 탈수화 반응을 진행하였다. 구조 유도제로는 테트라에틸암모늄수산화물(TEAOH), 디프로필아민(DPA), 디에틸아민(DEA), 몰포린(Morpholine) 그리고 디에탄올아민(DEtA)을 사용하였다. 촉매의 특성을 파악하기 위하여 질소 흡착-탈착 등온선, 암모니아 승온탈착($NH_3$-TPD), 그리고 승온산화분석(TPO)을 이용하였다. 구조 유도제로 몰포린(Morpholine)을 사용하여 제조한 SAPO-34를 촉매로 사용하였을 경우 사용한 촉매중에서 푸르푸랄 수율이 가장 높았다. 고체산 촉매의 외부 표면적과 산농도가 자일로오스의 탈수화 반응에 영향을 주는 것으로 나타났다.

• 생약학회지
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• 제35권1호통권136호
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• pp.6-15
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• 2004

This study was performed to evaluate the hemopoietic effects of 6 species of deer antlers from origins and parts in vitro. CD34 positive cells were isolated and confirmed the its population by FACS analysis. In a week liquid culture, there was any statistical significance between extracts of three parts of six species of deer antlers in the experiments as colony forming assay, proliferation assay, differentiation assay and observation of morphology. However, after 2 weeks- culture with extracts of three parts of six species of deer antlers, colonies were counted. six species of deer antlers, such as middle part of Korean nippon deer, upper part of Chinese nippon deer, upper part of Newzealand horse deer, middle part of Korea horse deer and middle part of Newzealand red deer, significantly increased the CFU-GM (colony forming unit garnulocyte-macrophage) of CD34 positive cells re1atεd to production of leucocytes such as eosinophil, basophil and neutrophil, while only middle part of Korea horse deer significantly increased the BFU-E (burst forming unit-erythroid) at 1 mg/ml seggesting progenting red blood cells (RBC). In the molecular study with CD34+ cells pretreated with cyclophosphamide, antagonist of hemopoietic activity, upper Part of Korean nippon deer and upper part of Chinese nippon deer effectively increased TPO involved in a late pathway of hematopoiesis just like in ELISA assay of IL-3, TPO and GM-CSF. Taken together, these results indicate exσacts of deer antler had some hemopoietic activity still proposing more clinical study and more basic mechanism research.

• 대한한방내과학회지
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• 제27권3호
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• pp.653-663
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• 2006

Objective : Graves' disease, the most common cause of hyperthyroidism, is an autoimmune disorder associated with autoantibodies to the TSH receptor. The clinical features of Graves' disease are goiter and hypermetabolic symptoms induced by excessive hormones. Antithyroid drug therapy is the first-line treatment for Graves' disease in Korea, Japan and European countries. Yet in spite of a long period and high-dose of treatment, it is hard to achieve remission because of adverse effects, frequent recurrence and resistance to antithyroid drugs. Recently, it has been reported that the abnormal thyroid hormone and clinical symptoms of Graves' disease were reduced by Ahnjeonbaekho-tang (AJBHT). Methods : To investigate the effectiveness and action mechanism of AJBHT, we studied the influence of AJBHT on FRTL-5 thyroid cell proliferation, DNA synthesis and expression of T4, TSH, cAMP, Tg and TPO mRNA. Results : AJBHT significantly inhibited the FRTL-5 cell proliferation, DNA synthesis, T4 synthesis, cAMP production and the expression of Tg mRNA in comparison with control and MMI. Conclusions : These results suggest that AJBHT may inhibit the cell proliferation and DNA synthesis by regulating the cAMP, and suppress the T4 synthesis by modulating Tg mRNA expression and cAMP synthesis, and that it may be useful agent for treating the goiter and hormone abnormality of Graves' disease.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제30권1호
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• pp.42-44
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• 2019

Hashimoto's encephalopathy (HE) is a rare and underdiagnosed neuropsychiatric illness. We present the case of a 17-year-old girl who was admitted to a tertiary-care psychiatric center with acute onset psychosis and fever. Her psychotic symptoms were characterized by persecutory and referential delusions, as well as tactile and visual hallucinations. Her acute behavioral disturbance warranted admission and treatment in a psychiatric setting (risperidone tablets, 3 mg/day). She had experienced an episode of fever with a unilateral visual acuity defect approximately 3 years before admission, which was resolved with treatment. Focused clinical examination revealed an enlarged thyroid, and baseline blood investigations, including thyroid function test results were normal. Abnormal laboratory investigations revealed elevated anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG) levels (anti-TPO of 480 IU/mL; anti-TG of 287 IU/mL). Results of other investigations for infection, including cerebrospinal fluid examination, electroencephalography, and brain magnetic resonance imaging were normal. She was diagnosed with HE and was treated with intravenous corticosteroids (methylprednisolone up to 1 g/day; tapered and discontinued after a month). The patient achieved complete remission of psychotic symptoms and normalization of the anti-thyroid antibody titers. Currently, at the seventh month of follow-up, the patient is doing well. This case highlights the fact that in the absence of well-defined clinical diagnostic criteria, a high index of suspicion is required for early diagnosis of HE. Psychiatrists need to explore for organic etiologies when dealing with acute psychiatric symptoms in a younger age group.

• Composites Research
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• 제35권1호
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• pp.38-41
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• 2022

고 변형률 속도에서 폴리프로필렌-유리 장섬유 복합재료(PP-LGF)와 열가소성 올레핀(TPO) 소재의 동적 압축 특성을 얻기 위해 홉킨슨바(Split-Hopkinson Pressure Bar (SHPB))를 이용하여 실험을 진행하였다. SHPB는 변형률 속도 100 s^-1~10000 s^-1 범위에서 재료의 동적 기계적 물성을 확인할 수 있는 장치이다. SHPB 시험은 입력봉과 전달봉에서 측정된 탄성파를 이용하여 시편의 응력, 변형률 및 변형률 속도를 얻을 수 있는 탄성파 전달 이론을 기반으로 한다. 또한 SHPB에서 얻은 변형률 데이터의 검증을 위해 시편을 초고속카메라로 촬영하여 DIC 기법을 통해 얻은 변형률 데이터와 비교 진행하였다.