• Korean Journal of Microbiology
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• v.42 no.4
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• pp.319-325
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• 2006

[ $\beta$ ]-Glucans (AG) were prepared from Agaricus blazei cultured in the medium fortified with the roots of Pueraria spp. by repeated extraction with hot water, gel filtration chromatography and DEAE ion exchange chromatography. Oligosaccharides (AO) were derived from the hydrolysis of AG by an endo-$\beta$-(1$\rightarrow$6)-glucanase from Bacillus megaterium. The anti-HT-29 human colon cancer activity of AG or AO was investigated using MTT assay, apoptosis assay, cell cycle analysis, and cDNA microairay. AG and AO both inhibited proliferation and growth of HT-29 cells, and stimulated apoptosis of the cells in a dose-dependent manner. In cell cycle analysis, treating HT-29 cells with AG or AO resulted in the increase of cells in the G0 (sub-G1) and G1 phase. Especially, AO was more effective in inducing G0/G1 cell cycle arrest than AG. To screen the genes involved in the increase of apoptosis, the gene expression profile of the HT-29 cells treated with AO was examined by cDNA microarray. While several genes involved in cell cycle progression (CCND2 and CDK2) were down-regulated, many genes involved in apoptosis (TNFSF9, TNFRSF9, FADD, CASP8, BAD, CRADD, CASP9 etc), cell cycle inhibitor (CDKN2A), immune response (IL6, IL18, IL6R etc), and tumor suppressor (CEACAM1, TP53BP2, IRF1, and PHB) were up-regulated. These results suggest that AO could inhibit the proliferation and growth of HT-29 cells by G0/G1 cell cycle arrest and induction of apoptosis.

• Korean Journal of Ecology and Environment
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• v.41 no.spc
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• pp.68-76
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• 2008

This study examined the inhibition effects of a freshwater bivalve (Unio douglasiae) and a submerged plant (Potamogeton crispus) on the cyanobacterial bloom (Oscillatoria sp.). The experiment were conducted in aquarium $(50cm{\times}65cm{\times}120cm)$ with lake sediments in the bottom of the aquarium in 10 cm thick. Before the experiments, artificial cyanobacterial bloom was induced with the addition of lake sediment and CB medium. Total 12 transparent acrylic cylinders (${\Phi}19cm$, height 40 cm) were placed in the aquarium, and within which bivalves and plants were placed in various conditions such as the control (C), plant addition (P:5 stems), mussel addition (U:2 individuals), and both mussel and plant addition (PU: the same quantity as used in each treatment). The experiment was conducted in triplicate during 7 days. pH, dissolved oxygen (DO), electric conductivity (EC), salinity, cyanobacterial cell density, chlorophyll-${\alpha}$ concentration, and mussel filtering rate were monitored daily. At the end of the experiment, total phosphorus (TP), total nitrogen (TN), and plant height and weight were measured. Overall, a large degree of cyanobacterial growth inhibition appeared in both P and U treatments, and the effect was highest in the U treatment, followed by P and PU. The combined treatment of both U and P did not show any synergic effects compared to the effect in separated treatment. In all enclosures of the treatments chlorophyll-${alpha}$ (Chl-${alpha}$) concentration decreased until 36 hours after the additions of the plants and mussels. In contrast, Chl-${alpha}$ concentrations increased in PU enclosures after 36 hours. The same trend was shown in the cell density of Oscillatoria. pH and DO gradually decreased until 120 and 144 hours, respectively, in the P and PU enclosures. TP concentration increased in the mussel enclosures (U and PU), while TN concentration largely decreased in the plant enclosures (P and PU). Our results suggest that applied bivalve (Unio) and submerged plant (Potamogeton) seemed to have a potential effect on the growth inhibition of cyanobacteria, but their combined application may have an antagonistic effect to diminish the degree of the inhibition.

• Journal of Animal Science and Technology
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• v.54 no.4
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• pp.275-282
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• 2012

The data including milk yields, fat and protein percent for 628,395 heads collected by National Agricultural Cooperative Federation, 15 type traits and final score for 62,262 heads collected by Korea Animal Improvement Association, which were born in 1998 to 2004, and net profits calculated from milk price and raising expenses of individuals were used to estimate genetic parameters. The highest positive genetic correlation, 0.81, was shown between body depth (BD) and loin strength (SR). Genetic correlations between body depth (BD) and udder depth (UD), front teat placement (TP) and front teat length (TL) were -0.23, which were lowest among the linear type traits. Furthermore, medium level of negative genetic correlations were shown the milk yield with milk contents rate traits. Mostly low level of positive genetic correlations were shown between the milk traits and linear score traits except milk yield and stature. Most of the genetic correlations of between the linear score traits and net profit were low level of positive or negative genetic correlations. Among the genetic correlations, body depth (BD), angularity (DF) and rear attachment width (UW), and final score (FS) with net profit were high as 0.17, 0.17, 0.18 and 0.18, respectively. Finally all of the genetic correlations between net profit and milk traits were positive and higher than the linear traits with positive genetic correlations. The results of this study suggest that net profit has been related with the linear traits, such as body depth (BD), angularity (DF) and rear attachment width (UW) traits, and furthermore, milk traits including yield and contents rates influence positively and greatly on net profit.

• Korean Journal of Weed Science
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• v.8 no.2
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• pp.182-186
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• 1988

This study was conducted to level up the tolerance of tobacco plant against bialaphos herbicide through tissue culture. The relatively good shoot regeneration from the subcultured calli treated with bialaphos at 0.5 ppm was observed in old the tobacco varieties tested such as NC 82, BY 4 and KA 101. However, at the treatment of bialaphos 1.0 ppm, shoot regeneration was only made in KA 101 variety, showing better regeneration than that of untreated one, When these shoots were transfered to the medium containing of bialaphos 10.0 ppm, the percentage of living shoots (i.e. tolerant plant) was very low, showing 2.43% in NC 82, 2.76% in KA 101 and 0.78% BY 4. Calli were induced and multiplied from leaf petiole of the above tolerant plants even under 2.5ppm of bialaphos, showing an average of 9% in NC 82 and 16% in KA 101 as compared with the untreated control. No calli were induced from tolerant plants as bialaphos concentration increased up to 5.0 ppm. Direct shooting from leaves of the above tolerant plants, that is selected at 10.0ppm of bialaphos treatment, was observed even under 10.0ppm of bialaphos treatment both in NC 82 and in KA 101 varieties, indicating that tolerance of tobacco plants against bialaphos can be greatly increased.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.3
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• pp.262-272
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• 2005

This study was undertaken to obtain design concentrations for the natural system treating stream water flowing into estuarine lake(Sapgyo). This lake has three major, so called, national-class streams: one is Gokgyo flowing through two medium-size municipal areas and the others are Muhan and Sapgyo placed in mostly rural area nested with some small towns. The results of three year's investigation showed big changes in water quality during the period between dry and wet seasons. Nitrogen concentration usually decreases as the flow rate increases. The change of phosphorus concentration in Gokgyo was the same pattern as nitrogen, but in other two streams it was inversely related with flow rates. This is probably due to the chemical property of the particles washed out from the different stream basins during wet season. It was found that about 40 to 60% of the total nitrogen are in soluble form and on average, 35% of the total phosphorus consists of dissolved-P. Representative concentration of these streams has to be separately determined based on dry and wet season. TDS and SS could be indicating parameters useful to depict and characterize a change of water quality with respect to flow rate because their measurement is easy and also includes a small analytical error. TDS values measured during dry season are related with high nitrogen concentration while during wet season, SS values effectively reflect a high concentration of phosphorus. For design purpose, cumulative concentration distribution graph presented in this paper can be directly or indirectly applied to other streams through a brief comparative and verificative study.

• Journal of Environmental Health Sciences
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• v.39 no.1
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• pp.48-55
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• 2013

Objective: The use of nanoparticle products is expected to present a potential harmful effect on consumers. Also, the lack of information regarding inhaled nanoparticles may pose a serious problem. In this study, we addressed this issue by studying pulmonary toxicity after nasal instillation of Al-NPs in SD rats. Methods: The animals were exposed to Al-NPs at 1 mg/kg body weight (low dose), 20 mg/kg body weight (medium dose) and 40 mg/kg body weight (high dose). To determine pulmonary toxicity, bronchoalveolar lavage (ts.AnBAL) fluid analysis and histopathological examination were conducted in rats. In addition, cell viability was investigated at 24 hours after the treatment with Al-NPs. Results: BAL fluid analysis showed that total cells (TC) count and total protein (TP) concentrations increased significantly in all treatment groups, approximately two to three times. Also, lactate dehydrogenase (LDH) and cytokines such as TNF-alpha and IL-6 dose-dependently increased following nasal instillation of Al-NPs. However, polymorphonuclear leukocytes (PMNs) levels showed no significant changes in a dose dependant manner in BAL fluid. In the cytotoxicity analysis, the treatment of Al-NPs significantly and dose-dependently induced cell viability loss (20 to 30%) and damage of cell membrane (5 to 10%) in rat normal lung epithelial cells (L2). Conclusions: Our results suggest that inhaled Al-NPs in the lungs may be removed quickly by alveolar macrophages with minimal inflammatory reaction, but Al-NPs have the potential to affect lung permeability. Therefore, extensive toxicity evaluations of Al-NPs are required prior to their practical application as consumer products.