• Title/Summary/Keyword: TORA

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Extraction Characteristics and Antioxidative Activity of Cassia tora L. Extracts (결명자 추출물의 추출특성 및 항산화 효과)

  • Na, Gyung-Min;Han, Ho-Suk;Ye, Su-Hyang;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Culture
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    • v.19 no.5
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    • pp.499-505
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    • 2004
  • The present study was conducted to investigate extraction characteristics and antioxidative activity of Cassia tora L. extracts. Cassia tora L. was extracted by reflux extraction under different extraction conditions including solvent. The solid yield, turbidity, color value, titratable acidity, free sugar contents, electron donating ability and superoxide dismutase-like ability of Cassia tora L. extracts were determined. The highest solid yield value was obtained with water of 10 fold. No significant difference in turbidity and color value were found among the extracts prepared with various extraction solvents, 75% ethanol, 50% ethanol and water. The highest titratable acidity was obtained with 50% ethanol of Cassia tora L.. The free sugar contents of Cassia tora L. extracted with water showed the highest value. Cassia tora L. extracts with water included higher contents of free sugar compared with then of the other solvent extracts, 50% ethanol and 75% ethanol extracts. The total polyphenol compound content of Cassia tora L. extracted with 50% ethanol showed the highest value. Cassia tora L. extracts with 50% ethanol included higher contents of total polyphenol compound compared with those of the other solvent extracts, water and 75% ethanol extracts.

Effect of Gamma Ray on Germination, Growth and Antioxidant Activity of Senna tora (감마선 조사가 결명자의 생육과 항산화 활성에 미치는 영향)

  • Um, Min;Kang, Si Yong;Lee, Jae Won;Lee, Ok Ran
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.5
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    • pp.290-295
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    • 2017
  • Background: Senna tora is a flowering plant in the legume family Fabaceae. Its seeds are roasted and consumed as tea in Asia, to reduce inflammation in the liver and improve eyesight. Thus, it has been considered as an important medicinal crops in Asia. However, breeding trials to improve its genetic properties are rare. Mutation breeding by gamma ray is known to be an effective and highly successful approach for the generation of agronomically useful cultivars. Here we analyzed the effects of several dosages of gamma ray on the biological conditions of Senna tora seeds. Methods and Results: The germination rate and growth patterns of Senna tora were examined following irradiation with gamma ray at 100, 200, 300 and 400 Gy. The total phenolic compound contents and antioxidant activities of Senna tora were analyzed. Germination increased at 100 and 200 Gy in the M1 and M2 generations compared with that of the control (M0). The total phenolic compound contents and antioxidant activity of the seeds significantly decreased as the radiation dosage increased above 100 Gy in the M1 generation. Conclusions: Senna tora, irradiated with gamma ray at dosages 100, 200, 300, and 400 Gy, showed maximum germination rate at 200 Gy in the M2 generation. Plant height and leaf size gradually decreased with increasing gamma ray intensity in the M2 generation. The total phenolic compound contents decreased significantly at 400 Gy, and the related antioxidant activity was also decreased as the radiation dosage increased.

Hypoglycemic Effect of Fractions of Cassia tora Extract in Streptozotocin-Induced Diabetic Rats (결명자 분획물이 당뇨 유발 흰쥐의 혈당에 미치는 영향)

  • 임숙자;한혜경
    • Korean journal of food and cookery science
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    • v.13 no.1
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    • pp.23-29
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    • 1997
  • The hypoglycemic effect of fractions of methanol extract of Cassia tora was investigated in diabetic rats. Sprague-Dawley male rats weighing 180∼230 g were divided into 6 groups. Diabetes was induced in the male rats by intravenous injection of streptozotocin at a dose of 45 mg/kg dissolved in citrate buffer (pH 4.5). The diabetic animals then had plasma glucose concentration of above 300 mg/㎗. Fractions of methanol extract of Cassia tora were administered orally into the diabetic rats for 14 days after streptozotocin injection. The food intake and body weight gain were monitored and plasma levels of glucose, triglyceride, free fatty acid, HDL-cholesterol, cholesterol and ALT activity were determined. Levels of glycogen, cholesterol and triglyceride in liver were analysed. Weights of liver and kidney were lighter in all the fraction-administered groups than streptozotocin-control group. Plasma glucose level was significantly decreased by the administration of butanol fraction at 14 days. Plasma triglyceride levels were lower in chloroform, butanol and H$_2$O fraction-administered groups compared to streptozotocin-control group. Plasma cholesterol and HDL-cholesterol levels were not significantly different in all groups. Administrations of each of the four fractions have decreased plasma free fatty acid level, ALT activity and liver triglyceride levels in the diabetic rats. Liver glycogen levels in chloroform, butanol and H$_2$O fraction-administered groups were higher than streptozotocin-control group. It is suggested from the results that butanol fraction of methanol extract of Cassia tora may contain the antihyperglycemic compounds.

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The NMR assignments of anthraquinones from Cassia tora

  • Choi, Jae-Sue;Jung, Jee-Hyung;Lee, Hee-Jung;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.19 no.4
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    • pp.302-306
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    • 1996
  • The $^1H- and^{13}C-NMR$ spectra of alaternin, aurantio-obtusin, chryso-obtusin, obtusin and 2-glucosyl obtusifolin isolated from the seeds of Cassia tora have been assigned based on HMBC, long-range HETCOR, fully $^1H-coupled {13}^C-NMR$, deuterium isotope experiment, and by comparison with the model compounds.

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A Rubrofusarin Gentiobioside Isomer from Roasted Cassia tora

  • Lee, Hee-Jung;Jung, Jee-Hyung;Kang, Sam-Sick;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.20 no.5
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    • pp.513-515
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    • 1997
  • From the roasted seeds of Cassia tora L., a new naphthopyrone glycoside was isolated and characterized as 10-[${\beta}$-D-glucopyranosyl-$(1{\rightarrow}6)-O-{\beta}-D-$glucopyranosyl)oxy]-5-hydrox y-8-methoxy-2-methyl-4H-naphtho[1, 2-b]pyran-4-one(isorubrofusarin gentiobioside). Along with isorubrofusarin gentiobioside, alaternin and adenosine were isolated and identified.

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Antihepatotoxic Activity of Cassia tora Leaf Extract

  • Maity, Tapan K.;Mandal, Subhash C.;Pal, M.;Saha, B.P.
    • Natural Product Sciences
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    • v.4 no.4
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    • pp.226-229
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    • 1998
  • Methanolic extract of Cassia tora leaves was evaluated for its hepatoprotective activity in rats by inducing hepatotoxicity with paracetamol (acute model). The extract at a dose of 400 mg/kg orally exhibited significant protective effect by lowering the serum levels of transaminase (SGOT and SGPT), bilirubin, and alkaline phosphatase (ALP). The effects produced were comparable to that of a standard hepatoprotective agent.

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Antimicrobial Effect of Emodin Isolated from Cassia tora Linn. Seeds against Food-Borne Bacteria

  • Lee, Na-Hyun;Lee, Sang-Min;Song, Da-Hyun;Yang, Ji-Yeon;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.56 no.3
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    • pp.187-189
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    • 2013
  • The antimicrobial activities of emodin and its derivatives (anthraquinone, alizarin, and alizarin-3-methyliminodiacetic acid) were evaluated using a paper disc diffusion method against foodborne bacteria (Bacillus cereus, Listeria monocytogenes, Staphylococcus intermedius, Salmonella typhimurium and Shigella sonnei). Emodin isolated from C. tora seeds has an antimicrobial activity against Bacillus cereus, followed by alizarin-3-methyliminodiacetic acid ($13.0{\pm}2.5mm$) and alizarin ($11.5{\pm}1.2mm$). Furthermore, emodin showed the antimicrobial activity against S. sonnei and S. typhimurium. In conclusion, C. tora seed and its active component derivatives are useful for the development of natural products on food supplemental agents and pharmaceuticals.

Dyeing properties of Protein Fabrics by Cassia tara Extracts (결명자 추출액에 의한 단백질계 섬유의 염색성)

  • 문명희
    • Journal of the Korean Society of Costume
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    • v.49
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    • pp.73-84
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    • 1999
  • The purpose of this study was to investigate dyeing properties of silk and wool fabric by Cassia tora extracts according to the conditions such as extraction(temperature time) dyeing(temperature time) condition kinds of mordants temperature of mordanting. The color fastness of laundering irradiation were examined. The results were as follows: The wavelength of maximum absorpthion by extracts appeared at 201, 277, 382nm in UV-Vis spectrum. Absorbance of extracts were increased with temperature and time. The extraction efficiency of Cassia tora was determined as $95^\circ{C}$ of extraction temperature 120 minutes of extraction time. Silk fabric had highest K/S value when dyeing for 90min at $40^\circ{C}$. Wool fabric had highest K/S value when dyeing for 60min at $80^\circ{C}$. Surface color of silk and wool fabric dyed with Cassia tora extracts was 3,9Y and 0.4Y but it was changed from 9.3YR to 7,4Y and from 9.4YR to 5Y by mordants. The fabrics of mordanted by metal ion dyed the depth of color. Laundering and irradiation fastness of mordanted fabrics were improved as compared with nonmordanted fabrics.

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Effect of Gamma Ray Irradiation on the Pretreatment and Enzymatic Hydrolysis of Senna tora Stalk (감마선 조사 처리에 의한 결명자 줄기의 전처리와 효소가수분해 효과)

  • Kim, Jo Eun;Gong, Sung Ho;Jung, Jin Tae;Lee, Ok Ran;Lee, Jae Won
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.2
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    • pp.127-133
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    • 2018
  • Background: The demand of recycling renewable agricultural by-products is increasing. Radiation breeding is a method used to improve saccharification efficiency. Thus, we investigated the effect of gamma ray irradiation on the pretreatment and enzymatic hydrolysis of the stalks of Senna tora, an important medicinal plants. Methods and Results: S. tora seeds were irradiated with gamma ray at doses of 100, 200, 300, and 400 Gy. In the pretreated biomass, glucan and lignin content were higher in the M1 ($1^{st}$ generations of irradiation) S. tora stalks than in the M2 ($2^{nd}$ generations of irradiation) stalks, this can be explained by the higher degradation rate in M1. After oxalic acid pretreatment, the concentration of total phenolic compounds (TPCs) in the hydrolysate increased in the gamma ray treated seeds. The highest relative increase rate in crystallinity in the pretreated biomass was observed in M1-400 Gy and M2-100 Gy. The cellulose conversion rate was higher in M1 than in M2, except for 200 Gy. Conclusions: Gamma ray irradiation at an appropriate dose can be used to improve the efficiency of pretreatment and enzymatic hydrolysis, thereby increasing biomass availability.

Brassinosteroid substances in immature Cassia tora seeds (결명자의 brassinosteroid 활성물질)

  • Park, Keun-Hyung;Kim, Seon-Jae;Hyun, Kyu-Hawn
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.99-104
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    • 1993
  • In order to explore the brassinosteroid-active component in Cassia tora, methanol extract of immature seeds was purified by sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as teasterone, castasterone, brassinolide by TLC and HPLC. Our work is probably the first report of endogenous brassinosteroid in Cassia tora. The content of brassinosteroid in Cassia tora as converted into brassinolide was $3.5{\sim}5.5\;ng/g$ fresh weight. The order of brassinosteroid contents was toward to be teasterone, castasterone, brassinolide.

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