• Journal of Life Science
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• v.23 no.2
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• pp.290-298
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• 2013

The genus Mycobacterium includes crucial animal and human pathogens such as Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium bovis. Although it is important to understand the genetic basis for their virulence and persistence in host, genetic analysis in mycobacteria was hampered by a lack of sufficient genetic tools. Therefore, many functional vectors as molecular genetic tools have been designed for understanding mycobacterial biology, and the application of these tools to mycobacteria has accelerated the study of mechanisms involved in virulence and gene expression. To overcome the pre-existing problems in genetic manipulation of mycobacteria, this paper reports new vector systems as effective genetic tools in Mycobacterium smegmatis. Three vectors were developed; pKOTs is a suicide vector for mutagenesis containing a temperature-sensitive replication origin (TSRO) and the sacB gene encoding levansucrase as a counterselectable marker. pMV306lacZ is an integrative lacZ transcriptional fusion vector that can be inserted into chromosomal DNA by site-specific recombination. pTnMod-OKmTs is a minitransposon vector harboring the TSRO that can be used in random mutagenesis. It was demonstrated in this study that these vectors effectively worked in M. smegmatis. The vector systems reported here are expected to successfully applicable to future research of mycobacterial molecular genetics.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.704-716
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• 2018

The aim of this study is to examine the characteristics analysis of the discharge and water quality based on TPLMS (Total Pollution Load Management System) in the Jinwi River unit basin, and to propose a management plan by selecting the point that needs improvement of water quality in order to achieve the target water quality. We evaluated the discharge and water quality characteristics, statistical analysis, daily delivery load and daily delivery density, grade classification, for 14 total pollution load site's from 2014 to 2016 year in Jinwi river unit basin. The average discharge of Jinwi river unit basin is $22.411m^3/s$ and discharge of Hwangguji River is 32.8% and the water quality characteristics along the tributary river were clarified spatially. As the result, it was analyzed that Seongeun River is an indirect indicator of organic pollutants, Gwanri River is a seasonal factor, Osan River and Hwangguji River both affect water quality. Estimation of delivered pollutant loads at the HG-3 site was 6,470.4 BOD kg/day, 6,846.7 TN kg/day and delivered pollutant loads density increased to $220.9BOD\;kg/day/km^2$, $22.4TP\;kg/day/km^2$ at the HG-4 site. This result demonstrates that the total pollution load site needed to improve water quality of the Jinwi River unit basin was HG-3 site.

• The Journal of Engineering Geology
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• v.19 no.3
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• pp.365-371
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• 2009

This paper address the characteristics of loading pollutants caused by the unit agricultural area to establish an efficient management method in NPS (non-point source). The relationship between rainfall and runoff shows good coefficient with 0.92, when the event which shows relatively long antecedent dry days is excepted. The impact of runoff volume on the runoff coefficient can be described by the rainfall intensity strongly. The pollutant EMCs (event mean concentrations) in runoff increased by the increase of antecedent dry days due to dry soil conditions. As the similar pattern of pollutant's loads such as TSS, BOD, COD, TN and TP, it is cleared that other pollutants can be removed when TSS is removed. Therefore the system using only runoff coefficients is not sufficient for the prediction of pollutant loads. It is necessary to consider soil conditions such as rainfall, antecedent dry day, antecedent rainfall etc. for the prediction system.

• Journal of Wetlands Research
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• v.21 no.2
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• pp.181-190
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• 2019

Recently, Low impact development techniques, a form of nature-based solutions (NBS), were seen cost-efficient alternatives that can be utilized as alternatives for conventional stormwater management practices. This study evaluated the effectiveness of an infiltration trench (IT) and a small constructed wetland (SCW) in treating urban stormwater runoff. Long-term monitoring data were observed to assess the seasonal performance and cite the advantages and disadvantages of utilizing the facilities. Analyses revealed that the IT has reduced performance during the summer season due to higher runoff volumes that exceeded the facility's storage volume capacity and caused the facility to overflow. On the other hand, the pollutant removal efficiency of the SCW was impacted by the winter season as a result of dormant biological activities. Sediment data also indicated that fine and medium sand particles mostly constituted the trapped sediments in the pretreatment and media zones. Sediments in SCW exhibited a lower COD and TN load due to the phytoremediation and microbiological degradation capabilities of the system. This study presented brief comparison LID facilities equipped with pre-treatment zones. The identified factors that can potentially affect the performance of the systems were also beneficial in establishing metrics on the utilization of similar types of nature-based stormwater management practices.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.2B
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• pp.225-231
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• 2006

The nonpoint source control is based on TPLMS (Total Pollution Load Management System) program. Recently, the Ministry of Environment in Korea has programed TPLMS for 4 major large rivers to improve the water quality in rivers by controling the total pollutant loadings from the watershed area. Usually the urbanization is the main pollutant sources, particularly for nonpoint pollutants, because of high imperviousness and high pollutant mass emissions. The stormwater runoff from urban areas is containing various pollutants such as sediments, metals and toxic chemicals due to human and vehicle activities. Of the various landuses, the highways are highly polluted landuses because of high pollutant accumulation rate by vehicle activities during dry periods. Therefore, this research is achieved to provide pollutant EMCs (Event Mean Concentrations) and mass loadings washed-off from highways during rainfall periods. Five monitoring locations were equipped with an automatic rainfall gage and an flow meter. The results show that the EMC ranges for 95% confidence intervals in highway land use are 45.52-125.76 mg/L for TSS, 52.04-95.48 mg/L for COD, 1.77-4.48 mg/L for TN, 0.29-0.54 mg/L for TP. The ranges of washed- off mass loading are $712.7-2,418.4mg/m^2$ for TSS and $684.1-1,779.6mg/m^2$ for COD.

• Journal of the Korea Organic Resources Recycling Association
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• v.32 no.1
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• pp.49-59
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• 2024

Biodegradable coating urea plays an important role in reducing the non-pollutants in agroecosystems, maximizing the plant nutrient utilization efficiency and slow-releasing nitrogen. Herein, the objective of this study was to investigate the nitrogen-releasing patterns and greenhouse gas emissions on different biodegradable coating urea. The treatments consisted of the control as an application of chemical fertilizers, NBCF as the non-biodegradable coating urea, NB60, and MDS as biodegradable coating urea. As a result of this study, the maximum accumulated total nitrogen (TN) concentration in the NBCF was higher at 33% than one in the NB60 during the precipitation periods. Its leaching period in the NCBF was prolonged for day 10 compared to the NB60. TN and NO₃-N releasing patterns in the NBCF and NB60 were fitted well on linear types(R²≥0.991), but their control and MDS were fitted well on Sigmoid curves(R²≥0.994) with high releasing concentration in the MDS compared to the control during leaching periods. For the greenhouse gas emissions, CH₄ emissions in the NBCF, NB60, and MDS were increased at 0.38%, 11.36%, and 5.91%, and N₂O emissions were also increased at 50.5%, 32.4%, 58.8% as compared to the control, respectively. Therefore, application of biodegradable polymer coating urea might mitigate the non-point pollutants in agro-ecosystem.

• Science of Emotion and Sensibility
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• v.6 no.2
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• pp.21-28
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• 2003

Tn the present study, 9 photographs from the International Affective Picture System (IAPS) judged to express best 2-dimensional sensibility were selected and used to stimulate the subjects. The goal of the study lay in verifying whether or not the consequent affective changes in the subjects can be discriminated psychologically and physiologically in terms of arousal and relaxation. In order to determine affective changes, the physiological signals of the subjects' autonomic nervous system were measured through electrocardiogram (ECG) , galvanic skin response (GSR), skin temperature, and respiration. After stimulation and measurement, subjective evaluation was conducted. Subjective evaluation revealed that affective arousal level could be classified into 4 consecutive stages: arousal 〉 unpleasant arousal, pleasant arousal 〉 comfort 〉 relaxation. Analysis revealed that the responses of the autonomic nervous system, too, could be classified into 3 consecutive stages: arousal, unpleasant arousal, pleasant arousal 〉 comfort 〉 relaxation. By selecting and using 9 photographs from the IAPS deemed best to express Koreans'2-dimensional sensibility, the present study confirmed that it is possible to discriminate changes in the subjects' affective arousal level in terms of arousal and relaxation through psychological and physiological evaluation.

• The Journal of the Korea Contents Association
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• v.5 no.1
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• pp.201-208
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• 2005

Tn this paper, we evaluate the peak-to-average power ratio (PAPR) performance in a space-time block code (STBC) multi-input multi-output orthogonal frequency division multiplexing (MIMO-OFDM) system using selected mapping (SLM) and partial transmit sequences (PTS) approaches. SLM and PTS methods are used to decrease the nonlinear distortion and to improve the power efficiency of the nonlinear high power amplifier(HPA) in the MIMO-OFDM system. In simulation result, when compared with the existing MIMO-OFDM system using QPSK, the PTS method reduces the PAPR about 5dB while the SLM method can reduce about 3.5 dB. Also, we find the BER performance of the MIMO-OFDM system with and without the predistorter in front of the HPA. When the predistorter is used, the input back-off (IBO) of 4 dB is required in the PTS method, and IBO of 6 dB in the SLM method to closely conform to the linear amplifier. If the method of improving the PAPR is not used, the value of IBO of 8 dB is required.

• Journal of the Korean Institute of Intelligent Systems
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• v.12 no.4
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• pp.334-340
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• 2002

This paper proposes a self-organizing fuzzy modeling which can create a new hyperplane-shaped cluster by applying multiple regression to input/output data with relatively large fuzzy entropy, add the new cluster to fuzzy rule base and adjust parameters of the fuzzy model in repetition. Tn the coarse tuning, weighted recursive least squared algorithm and fuzzy C-regression model clustering are used and in the fine tuning, gradient descent algorithm is used to adjust parameters of the fuzzy model precisely And learning rates are optimized by utilizing meiosis-genetic algorithm. To check the effectiveness and feasibility of the suggested algorithm, four representative examples for system identification are examined and the performance of the identified fuzzy model is demonstrated in comparison with that of the conventional fuzzy models.

• Journal of Environmental Impact Assessment
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• v.13 no.4
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• pp.153-164
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• 2004

An integration study of time-variable small watershed and stream models (USEPA's SWMM and WASP5) was performed for impact assessment of urbanization on water environment. The study area, the Kyoungan Stream, the tributary of Paldang Lake, was divided into 111 subbasins, based on the topographic condition, land use, and drainage system. RUNOFF block of SWMM was applied to estimate runoff flow and quality. EXTRAN block computed daily and hourly flow according to simulated runoff flow, water supply, and drainage data. SWMM was connected to WASP5 by transforming output file of SWMM into input file of WASP5. The nonpoint source loads and flow data of SWMM were imported to WASP5. The stream was divided into 45 segments based on the watershed delineation. The study included three water quality parameters, BOD, TN, and TP. The validate models were used to examine the impact of urbanization on stream flow and water quality.