• 교통기술과정책
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• 제7권1호
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• pp.47-52
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• 2010

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2007년도 한국우주과학회보 제16권2호
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• pp.96.1-96.1
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• 2007

• Asian Pacific Journal of Cancer Prevention
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• 제13권2호
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• pp.641-646
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• 2012

In our recent report on gene expression in gastric cancer we identified the endo-sulfatase Sulf-1 gene to be up-regulated in gastric tumors relative to apparently normal (AN), and paired normal (PN) gastric tissue samples. In the present report we investigate the protein expression levels of Sulf-1 gene in gastric tumors, AN and PN samples using tissue microarray (TMA) and immunohistochemistry. Expression data was collected from two sets of TMA's containing replicate sections of tissue samples. Scoring data from TMA set-1 revealed a significant difference in Sulf-1 immunoreactivity between tumors and "normals" (PN and AN) (p-value = 0.001928). Also, Sulf-1 expression in tumors was also significantly different from either PN (p-value = 0.019) or AN (p-value = 0.006) samples. Similar results were obtained from analysis of scoring data from the second set of arrays. Comparison of mRNA expression and protein expression in gastric tumor tissues revealed that in 6/20 (30%) tumor samples showed up-regulated protein expression concordant with over-expression of mRNA. However, a discord with mRNA being over-expressed relative to down regulated protein expression was observed in majority 14/20 (70%) of tumor samples. Our study indicates down regulation of Sulf-1 protein expression in gastric tumors relative to PN and AN samples which is discordant with mRNA over-expression seen in tumors.

• 한국대기환경학회지
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• 제23권1호
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• pp.39-49
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• 2007

In this study, the performance characteristics of solid phase microextraction (SPME) were investigated for three major odorous groups that consist of 10 individual compounds ([1] volatile organic compounds (VOC): benzene, toluene, p-xylene and styrene, [2] reduced sulfur compounds (RSC): hydrogen sulfide, methyl mercaptan, dimethylsulfide (DMS), dimethyldisulfide (DMDS), and carbon disulfide, and [3] amine: trimethylamine (TMA)). For the purpose of a comparative analysis, two types of SPME fiber ([1] polidimethylsiloxane/divinilbenzene (P/D) and [2] $Carboxen^{TM}$/polidimethylsiloxane (C/P)) were test ε d against each other for a series of standards prepared at different concentration levels (100, 200, and 500 ppb). To compare the analytical performance of each fiber, all standards were analyzed for the acquisition of calibration data sets for each compound. The results of P/D fiber generally showed that its calibration slope increased as a function of molecular weight across different VOCs; however, those of C/P fiber showed a fairly reversed trend. Besides, we confirmed that the application of SPME is limited to many sulfur compounds; only two compounds (DMS and DMDS) are sensitive enough to draw calibration results out of SPME. The calibration data for RSC show generally enhanced slop values for C/P relative to P/D fiber. However, in the case of TMA, we were not able to find a notable difference in their performance.

• 한국수산과학회지
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• 제15권2호
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• pp.117-122
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• 1982

오분자기의 정미성분에 관한 자료를 얻고자 핵산관연물질, 유리아미노산, TMAO, TMA 및 총creatinine을 분석하였다. 핵산관련물중 AMP가 가장 많아서 $2.21{\mu}mole/g$이었으며 IMP와 inosine은 흔적량이었다. 유리아미노산중 함양이 많은 것은 taurine, arginine 및glycine으로 이들 3종 아미노산이 전유리아미노산의 $79.3\%$를 차지하였으며 tyrosine과 phenyl alanine은 흔적량이었다. TMAO, TMA 및 총 creatinine의 사양은 각각 $5.4mg\%,\;1.0mg\%$, 그리고 $5.0mg\%$ 였다. Omission test 결과 오분자기의 정미성분은 유리아미노산이 그 주체를 이루고 여기에 유기산과 핵산관련물질이 보조적인 역할을 하는 것으로 생각된다.

• Toxicological Research
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• 제19권2호
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• pp.133-139
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• 2003

Allergic contact dermatitis may be caused by a wide variety of chemicals. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. However, there is a need to develop a nonradioisotopic endpoint for the LLNA, because of the radioisotopic method's requiring the use of special facilities. In this study, we investigated the development of a nonradioisotopic endpoint for LLNA using ELISA (enzyme-linked immunosorbent assay). Female Balb/c mice were treated by the topical application on the dorsum of both ears with four different strong sensitizers, 2,4-dinitrochlorobenzene (DNCB), oxazolone (OXZ), toluene diisocyanate (TDI), and trimellitic anhydride (TMA), and a strong irritant, sodium lauryl sulfate (SLS), once daily for three consecutive days. The proliferation of cells in the auricular Iymph node was analyzed by means of the labelling index (Ll) of bromodeoxyuridine (BrdU) incorporation into cells. The weights of the Iymph nodes in the mice treated with allergens, DNCB, OXZ, TDl and TMA were increased compared to the vehicle control. The stimulation index (Sl) of mice treated with DNCB, OXZ, TDl, and TMA was over three-fold increase compared to the vehicle control. However, the S1 of mice exposed to SLS was not significantly increased compared to the vehicle control, while the lymph node weight of SLS was significantly increased. These results suggest that the LLNA modified endpoint using ELISA based on BrdU incorporation could provide a useful method of screening for irritants and allergens.

• Nuclear Engineering and Technology
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• 제1권1호
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• pp.23-31
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• 1969

젓새우(Acetes chinensis), 피둥어꼴뚜기 (Todarodes Pacifcus, 속칭 동해안산의 오징어), 젓꼴뚜기 (Loligo beka)를 1 Mrad까지의 각 성량의 ${\gamma}$선을 조사하여 이것들을 2~4$^{\circ}C$에서 저장하여 방사선에 의한 저장효과를 시험하였다. 원료와 저장중의 어체선도는 관능검사, 화학적 검사 (VRS, TMA-N량), 생화학적검사 (Hypo-xanthine 량) 및 세균학적검사 (생균수)에 의거하여 판정하였다. VRS 값은 3어종에 있어서 모두 관능검사치와 잘 부합되지는 않으나 TMA-N, Hypoxanthine 식 및 생균수는 관능검사치와 잘 부합되며, 각 어종의 선도판정치로서 이용할수 있다. 비조사 젓새우는 3일간의 저장이 가능하나 최적선량 0.12-0.20 Mrad에서 8일간까지 저장할 수 있고, 피둥어꼴뚜기는 8일간의 저장을 최적선량 0.20-0.30 Mrad 에서 14-21일간의 저장이 가능하였으며, 젓꼴뚜기는 5-6일의 저장기간이 최적선량 0.12-0.20 Mrad에서 약 10일간의 저장이 가능하였다.

• 동아시아식생활학회지
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• 제6권2호
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• pp.205-211
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• 1996

겨자의 항균성 및 식품보존제로의 이용 가능성을 조사하기 위하여 겨자의 처리에 따른 전어회의 보존효과를 조사하고, Vibrio Parahaemolyticus의 형태변화를 현미경으로 관찰한 결과는 다음과 같다. 겨자를 농도별로 첨가시킨 물에 담근 전어의 pH는 보존기간이 지남에 따라 미량 증가하였으며, 겨자 첨가농도가 높을수록 낮게 나타났고, 생균수 및 Vibrio 균수는 겨자 무첨가구에 담근 전어에서보다 겨자 첨가구에 담근 전어에서 감소하였으며, 겨자첨가농도가 높을수록 감소하는 폭이 크게 나타났고, TMA 질소 함량은 보존기간이 지남에 따라 점차 증가하였으며, 겨자 첨가농도가 높을수록 감소하는 경향이었다. 겨자 물추출물로 처리한 Vibrio Parahaemolyticus를 전자현미경으로 관찰한 결과 균체가 팽윤되고, 표층구조가 허물어져 심한 형태학적 변화를 나타내었다.

• 대한족부족관절학회지
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• 제9권1호
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• pp.9-12
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• 2005

Purpose: This study is to compare the medial longitudinal arch between heel pain group and normal painless group. Materials and Methods: Heel pain group 242 feet and normal group 140 feet were evaluated through the radiographic images of standing foot-ankle lateral view. Four radiographic indices, talo-1stmetatarsal angle (TMA), calcaneal-1st metatarsal angle (CMA), calcaneal pitch angle (CPA), and arch ratio (AR), were used as well as BMI. Results: There was no difference between heel pain group and normal group in the TMA, CMA, CPA, and AR. But in the BMI, heel pain group showed $1.7\;kg/m^2$ (p=0.0002) higher than normal group. To eliminate the sexual error, male and female were evaluated separately. Male heel pain group showed 2.9 degrees more dorsiflexion (p=0.001) in the TMA, 3.1 degrees greater (p=0.007) in the CMA, 0.01 lower (p=0.028) in the AR, and $1.0\;kg/m^2$ greater (p=0.033) in the BMI than normal male group. There were no difference in the CPA. Female heel pain group showed 3.6 degree greater (p=0.035) in the CMA, and $1.9\;kg/m^2$ greater (p=0.002) in the BMI than normal female group. But other indices demonstrated no differences. Conclusion: talo-$1^{st}$ metatarsal angle, calcaneal-$1^{st}$ metatarsal angle and arch ratio were radiographic indices related with heel pain.

• 한국축산식품학회지
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• 제35권3호
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• pp.398-405
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• 2015

Egg allergy has been shown to be the most common food allergy in children with atopic dermatitis. Allergic reactions to proteins derived from egg white (EW) are more common than those derived from egg yolk. Ovomucoid, ovalbumin, ovotransferrin, and lysozyme have been identified as major allergens in EW. This study was conducted to evaluate the effect of EW on immune modulation in an induced allergy mouse model. A total of 50 five-week-old BALB/c male mice were treated with trimellitic anhydride (TMA) for three weeks to induce allergy-like symptoms. The TMA-treated mice were rested for one week and then divided into five groups and fed 0, 10, 50, and 100 mg/d EW for four weeks. All EW consumption groups showed no significant increase or decrease in the populations of white blood cells; however, a significant increase in B-lymphocyte activity was observed in the fourth week. Furthermore, EW consumption did not influence serum immunoglobulin G and immunoglobulin E levels. Taken together, these data demonstrate that the consumption of EW by TMA-treated mice did not increase allergic parameters such as serum IgE level, but enhanced the lymphocyte activities against pathogens. Therefore, this study suggests that the consumption of EW promotes Th2 immune modulation, and EW could be an excellent candidate for maintaining health.