• Analytical Science and Technology
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• v.28 no.2
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• pp.79-85
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• 2015

A preliminary experiment was performed to develop a fast, convenient, and economical semi-quantitative method of analyzing amphetamine-like amines from images of derivatives. These were generated from the reaction (in situ, co-spot) of three amphetamine-like compounds with three derivatization reagents on a TLC plate. The attempt was made to optimize the reaction conditions for an efficient derivatization reaction, and TLC images taken by a digital camera were analyzed using two types of image analysis program (CP Atlas 2.0 and ImageJ) for repeatability (RSD, %) and linearity (R²). Then, their results were compared. For efficient derivatization, the reaction conditions needed to be modified. The results of image analysis of each of the samples at two different concentrations (0.5 mg/mL and 0.01 mg/mL) showed that the RSD values for reaction repeatability were in the range of 0.69-5.50%. From the calibration curves between the area of the derivative and the concentration of amines, the R² values (R² > 0.9906) for good linear correlation were found to be high, in a concentration range of 0.1-0.005 mg/mL of amines. In addition, the two programs demonstrated little difference in the analysis of repeatability and linearity of the derivatization, so that the current method has the potential to be used for the semi-quantitative analysis of amines.

• Journal of Power System Engineering
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• v.20 no.6
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• pp.51-57
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• 2016

Experiment is carried out to investigate the mixed convective flow in three-dimensional horizontal rectangular channels filled with high viscous fluid. The particle image velocimetry(PIV) with thermo-sensitive liquid crystal tracers is used for visualizing and analysis. Quantitative data of temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. In this study, the fluid used is silicon oil(Pr=909), the aspect ratio(channel width to heigh) is 4 and Reynolds number is $2{\times}10^{-2}$. From the present study, we can visualize the quantitative temperature and velocity of mixed convective flow in three-dimensional horizontal rectangular channels simultaneously.

• Journal of Power System Engineering
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• v.9 no.2
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• pp.33-39
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• 2005

Experiments are performed to study the mixed convection flow and heat transfer in an inclined parallel plates with the upper part cooled and the lower part heated uniformly. The Reynolds number ranges from $4.0{\times}10^{-3}\;to\;6.2{\times}10^{-2}$, the angle of inclination, ${\theta}$, from 0 to 45 degree from the horizontal line, and Pr of the high viscosity fluid is 909. In this paper, the PIV(Particle image velocimetry) with TLC(Thermo-sensitive liquid crystal) tracers is used for visualizing and analysis. This method allows simultaneous measurement of velocity and temperature field at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. This paper describes the methods, and presents the quantitative visualization of mixed convection. From this study, it is found that the flow pattern can be classified into three patterns which are affected by Reynolds number and the angle of inclination.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.18 no.9
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• pp.698-706
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• 2006

Experiment and numerical calculation have been peformed to investigate mixed convection heat transfer between inclined parallel plates. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualizing and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. The governing equations are discretized using the finite volume method. The results are presented for the Reynolds number ranges from 0.004 to 0.062, the angle of inclination, ${\Theta}$, from 0 to 45 degree and Prandtl number of the high viscosity fluid is 909. The results show velocity, temperature and mean Nusselt numbers distributions. It is found that the periodic flow of mixed convection between inclined parallel plates is shown at $0^{\circ}{\leq}{\Theta}<30^{\circ}$, Re<0.062, and the flow pattern can be classified into three patterns which depend on Reynolds number and the angle of inclination. The minimum Nusselt numbers occur at Re=0.05 regardless of the angle of inclination.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1071-1075
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• 2003

In a closed square cavity filled with a liquid, a cooled the upper horizontal wail and a heated the lower horizontal wall, the flow isn't generated under the ground-based condition when Rayleigh number is lower than 1700. In such case the flow phenomena near an air bubble under a cooled horizontal wall were investigated. The temperature and the flow fields were studied by using the Thermo-sensitive Liquid-Crystal and the image processing. The qualitative analysis for the temperature and the flow fields were carried out by applying the image processing technique to the original data. Injecting bubble at the center point of upper cooled wall, the symmetry shape of two vortexes near an air bubble was observed. The bubble size increased, the size of velocity and the magnitude of velocity increased. In spite of elapsed time, a pair of two vortexes was the unique and steady-state flow in a square cavity and wasn't induce to the other flow in the surround region.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.7
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• pp.987-993
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• 2009

An experimental study is performed to investigate unsteady mixed convection in a horizontal channel with a heat source. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualization and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. It is found that the periodic flow of mixed convection in a cavity appears at very low Reynolds numbers (Re<0.4), and the period decreases with increasing Reynolds numbers and increases with increasing aspect ratio.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.85-89
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• 2003

In a closed square cavity filled with a liquid, a cooled the upper horizontal wall and a heated the lower horizontal wall, the flow isn't generated under the ground-based condition when Rayleigh number is lower than 1700. In such case the flow phenomena near an air bubble under a cooled horizontal wall were investigated. The temperature and the flow fields were studied by using the Thermo-sensitive Liquid-Crystal and the image processing. The qualitative analysis for the temperature and the flow fields were carried out by applying the image processing technique to the original data. Injecting bubble at the center point of upper cooled wall, the symmetry shape of two vortexes near an air bubble was observed. The bubble size increased, the size of velocity and the magnitude of velocity increased. In spite of elapsed time, a pair of two vortexes was the unique and steady-state flow in a square cavity and wasn't induce to the other flow in the surround region.

• Applied Chemistry for Engineering
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• v.29 no.3
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• pp.342-349
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• 2018

In this study, the physicochemical properties, emulsifying capacity, moisture content and cytotoxicity of the composite material produced by transesterification reactions of the olive oil (olive oil esters) were investigated for cosmetic applications. Olive oil esters with short (S) and long (L) reaction times were studied. From the TLC-image analysis, composition ratios of the olive oil esters S were found to be 5.2, 24.1, 46.4, and 21.9% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. Those of the olive oil esters L were 4.1, 24.7, 40.6, and 28.8% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. The iodine value, acid value, saponification value, unsaponified matter, refractive index, and specific gravity were determined and purity tests were also carried out and normalized to establish standards and testing methods for using olive oil esters in cosmetics. To evaluate their emulsifying capacities, the O/W emulsion was prepared without surfactants and the formation of the emulsified particles were confirmed. After 5 days of applying the olive oil esters to human skin, the skin moisture retention was improved by 13.1% from the initial state. For the evaluation of toxicity on human skin cells, the olive oil esters showed 90% or more of the cell viability at $0.2-200{\mu}g/mL$. These results suggested that olive oil esters can be applied as natural/non-toxic ingredients to cosmetics industries.

• Korean Journal of Microbiology
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• v.35 no.2
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• pp.146-152
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• 1999

Acceplor reaction of glucosyltr~ansferase of Streptococcus ,SO~~-~IZLIS with f ~ ~ t o ~ y l o l i g o ~ a ~ ~ h a r i d e ~ was studied for the biosynthesis of novel olgisaccharides. First, bacilracin resistant mutants were selected by mutagenesis of Streptococcus sobrimis ATCC27351. Among these mutants 4 strains were selected by resistance to bacitracin and increase of glucosyltransferase. Acceptor reaction of maltose was analyzed by TLC and image analysis. There were differences in the specificity of the acceptor reaclion by Ule glucosylumsferase between mother strain (Streptococcus sobrinus ATCC2735) and bacitracin resistant mutants (Streptococcus sobrinus BR24C, Strepfococcus sobrinus CH-5). Molher strain did ilot show an acceptor reaction with fructosyloligosaccharides such as 1-keqtose and nystose. Acceptor reaction products of turailose and 1-kestose with glucosyltransferase (GW-S) of Streptococcus sobrini~s BR24C were TEX>\6^{3}$-$\alpha$-D-glucopyranosyl \3^{2}$-O-$\alpha$-D-fructose (glucose-fructose-glucose) and \6^{4}$-$\alpha$-D-glucopyranosyl \1^{3}$-$\beta$-D-~-h~ctofuranos~~ sucrose (glucose-glucosefructose- fructose). respectively These are novel oligosaccharides which can be produced only by enzymatic reaction.