• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.3
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• pp.1114-1122
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• 1996

Variation of temperature field in a Hele-Shaw convection cell was measured by using a HSI true color image processing system and TLC(Thermochromic Liquid Crystal) solution. The relationship between the hue value of TLC color image and real temperature was obtained and this calibration result was used to measure the true temperature. The temperature field in the Hele-Shaw convection cell shows periodic characteristics of 45 sec at Ra = 9.3 * 10$\^$6/. The temperature field measurement technique developed in this study was proved to be a useful and powerful tool for analyzing the unsteady thermal fluid flows.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.10
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• pp.3334-3343
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• 1996

A quantitative flow visualization technique was developed to measure velocity and temperature fields simultaneously in a two-dimensional cross section of thermo-fluid flows. Thermochromic liquid crystal(TLC) particles are used as temperature sensor and velocity tracers. Illuminating a thermo-fluid flow with a thin sheet of white light, the reflected colors from the TLC particles in the flow were captured simultaneously by two CCD cameras; a 3-chip CCD color camera for temperature field measurement and a black and white CCD camera for velocity field measurement. Variations of temperature field were measured by using a HSI true color image processing system and TLC solution. The relationship between the hue values of TLC color image and real temperature was obtained and this calibration curve was used to measure the true temperature under the same camera and illumination condition. The velocity field was obtained by using a 2-frame PTV technique using the concept of match-probability to track true velocity vectors from two consecutive image frames. These two techniques were applied at the same time to the unsteady thermal-fluid flow in a Hele-Shaw cell to measure the temperature and velocity field simultaneously and some results are discussed.

• Biomolecules & Therapeutics
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• v.1 no.1
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• pp.58-64
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• 1993

DNA addicts induced by putative chemical related to carcinogenesis were detected and determined by $^{32}$P-Postlabelling assay after exposure of 4 compounds comprising two auto dyes (amaranth, new coccine) and two flavonoid compounds (rutin, quercetin) to ICR mouse. DNA was isolated from mouse liver and digested enzymatically to deoxyribonucleoside 3'-monophosphate. The postincubation of DNA digests with nuclease Pl before $^{32}$P-labelling enhanced the technique's sensitivity. Nuclease Pl cleaves deoxyribonucleoside 3'-mono-phosphates of normal nucleotides to deoxyrihonucleosides which do not serve as substrates for polynucleotide kinase, while most of addicts were found to be totally or partially resistant to the 3'-dephosphorylating action of nuclease Pl. The adducted deoxyribonucleoside 3'-monophosphate was converted to 5'-$^{32}$P-labelled deoxynucleoside 3',5'-bisphosphate by T4 polynucleotide kinase. The nucleotides were separated by anion-exchange thin layer chromatography(TLC) on polyethyleneimine cellulose by 4-dimensional or 2-dimensional TLC then detected by autoradiography. The results show that DNA addicts were detected in liver DNA of ICR mouse after administration of amaranth and quercetin by 2-dimensional and/or 4-dimensional TLC.

• 한국가시화정보학회:학술대회논문집
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• 2004.11a
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• pp.52-55
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• 2004

Thermo-chromic Liquid Crystal(TLC) particles were used as temperature sensor for thermal fluid flow. $1K\times1K$ CCD color camera and Xenon Lamp(500W) were used for the visualization of a Hele-Shaw cell. The characteristic between the reflected colors from the TLC and their corresponding temperature shows strong non-linearity. A neural network known as having strong mapping capability for non-linearity is adopted to quantify the temperature field using the image of the flow. Improvements of color-to-temperature mapping was attained by using the local color luminance (Y) and hue (H) information as the inputs for the constructed neural network.

• KSBB Journal
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• v.17 no.3
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• pp.302-306
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• 2002

The chemical analysis and surface chemical properties of biosurfactant formed by Rhodococcus sp. strain IGTS8, which is widely used in biodesulfurization process, in hexadecane/water mixture have been studied. For the chemical analysis, TLC technique was employed. The surface tension, CMC, and emulsion stability of biosurfactant solution were also investigated. The major components of biosurfactant formed by Rhodococcus sp. strain IGTS8 were glucose mycolate and trehalose monomycolate. The CMC of aqueous biosurfactant solution was 0.1 ~0.15 g/100 mL of Water at pH 6.0-6.5 and pH 10~10.5. But the demulsification was faster at pH 10 than at pH 6.3.

• YAKHAK HOEJI
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• v.20 no.1
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• pp.44-46
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• 1976

Fourteen species of umbelliferae-plants grown in Korea were examined for the presence of polyacetylenic compounds. By using the UV and IRspectrometric technique combined with TLC, the presence of polyacetylenic compounds was identified in Phellopterus littoralis $B_{ENTHAM}$ Peucedanum japonicum $T_{HUNBERG}$ and Angelica koreana $M_{AXIMOWITZ}$.

• Journal of Food Hygiene and Safety
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• v.11 no.2
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• pp.149-157
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• 1996

A procedure for the determination of Aflatoxins in food and grains which utilizes reversed phased liquid chromatographic (LC) analysis with postcolumn derivatization by an electrochemical cell and determination with a fluorescence detector has been evaluated. The LC mobile phase was water-acetonitrile-methanol (6+2+2) with 1mM KBr and 1 mM HNO3 which gave baseline separation for the four Aflatoxins (AfB1, AfB2, AfG1, AfG2). The electrochemical cell set at 7V, generated bromine and derivatized aflatoxins B1 and G1, The derivatives were detected by the fluorescence detector. The aflatoxins in naturally contaminated corn samples were isolated by three different cleanup procedures: the AOAC method I column(CB method), a rapid filtrate column (Romer's column), and an immunoaffinity column. The final extract were quantitated with fluordensitometric TLC and the LC postcolumn derivatization techniques. The results were quite similar, however the LC technique showed less interferences and could be automated. Samples of corn, raw peanuts, peanut butter and dried dates were also analyzed successfully with this procedure.

• Transactions of the Korean Society of Automotive Engineers
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• v.5 no.1
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• pp.120-128
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• 1997

The variations of the temperature field in a passenger compartment were measured by using a HSI true color image processing system and TLC(Thermochromic Liquid Crystal) solution. This temperature measurement technique was proved to be useful for analyzing the ventilation flow. The flow field in the passenger compartment was visualized using a particle streak method with pulsed laser light sheet. The temperature field and flow field in the passenger copartment were affected significantly by the ventilation mode. The panel-vent mode heating had shorter elapse time to reach a uniform temperature than the foot-vent mode under the same ventilation condition and nonuniformity inside the passenger compartment could be minimized effectively by using the bilevel heating mode. The temperature increase rate in the rear passenger compartment was iower than the front compartment, especially in the vicinity of the rear seat occupants' knee level.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2352-2356
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• 2008

Heat transfer and performance characteristics have been investigated for a rectangular wavy duct with dimple or protrusion arrays. The test duct was 15mm in height and 105mm wide. The print diameter of the dimple/protrusion wall was 12.99mm and the depth/height of the dimple/protrusion was 3.75mm. Local heat transfer coefficients on the dimple/protrusion wall were measured using a transient TLC technique. The Reynolds number was varied from 3,000 to 10,000. For the wavy duct tested in this study, adverse static pressure characteristics occurred at turning region of the wavy duct due to secondary flows. For the wavy duct with protrusion array, higher heat transfer enhancement level of 7.4 times than smooth straight case in maximum was obtained at low Reynolds number due to the high heat transfer enhancement by vortex flows. Also, the protrusion array increased the performance level of 3.0 at low Reynolds number of 3,000.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.2
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• pp.209-216
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• 2005

Thermo-chromic Liquid Crystal(TLC) particles were used as temperature sensor for thermal fluid flow. 1K $\times$ 1K CCD color camera and Xenon Lamp(500w) were used for the visualization of a Hele-Shaw cell The characteristic between the reflected colors from the TLC and their corresponding temperature shows strong non-linearity A neural network known as having strong mapping capability for non-linearity is adopted to quantify the temperature field using the image of the flow. Improvements of color-to-temperature mapping was attained by using the local color luminance (Y) and hue (H) information as the inputs for the constructed neural network.