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Higher Biomass Production of Lactobacillus bulgaricus NLS-4 by Improvement of Cultural Conditions (배양조건 개선에 의한 Lactobacillus bulgaricus NLS-4의 균체 생산성 향상)

  • Rhee, Sang-Ki;Pack, Moo-Young
    • Microbiology and Biotechnology Letters
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    • v.8 no.1
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    • pp.55-60
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    • 1980
  • Some of the cultural conditions were improved in order to obtain the higher biomass of Lactobacillus bulgaricus NLS-4 which has the higher lactic acid producing activity as well. Among eight media including 11% non-fat milk medium as a control, the TIP medium was selected. By a batch experiment, the maximum cell concentration could be increased to 1.0$\times$10$^{9}$ cells per $m\ell$ when the organism was grown at 38$^{\circ}C$ for 18 hours with agitation speea of 200 rpm and under the constant level of pH 6.5 con-trolled with 1 N KOH solution in the selected medium. The cell concentration was further increased to 2.3$\times$10$^{9}$ cells per me in the steady state of continuous culture at the dilution rate of 0.17 hr$^{-1}$ for 18 hours.

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Application of Gurney Flaps on a Centrifugal Fan Impeller

  • Dundi, Thomas Manoj Kumar;Sitaram, Nekkanti;Suresh, Munivenkatareddy
    • International Journal of Fluid Machinery and Systems
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    • v.5 no.2
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    • pp.65-71
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    • 2012
  • The objective of the present investigation is to explore the possibility of improving the performance of a centrifugal fan at low Reynolds numbers using a simple passive means, namely Gurney flap (GF). GFs of 1/$8^{th}$ inch brass angle (3.175 mm) corresponding to 15.9% of blade exit height or 5.1% of blade spacing at the impeller tip are attached to the impeller blade tip on the pressure surface. Performance tests are carried out on the centrifugal fan with vaneless diffuser at five Reynolds numbers (viz., 0.30, 0.41, 0.55, 0.69, $0.82{\times}10^5$, i.e., at five speeds respectively at 1,100, 1,500, 2,000, 2,500 and 3,000 rpm) without and with GF. Static pressures on the vaneless diffuser hub and shroud are also measured for each speed at four flow coefficients [${\phi}$=0.23 (below design flow coefficient), ${\phi}$=0.34 (design flow coefficient), ${\phi}$=0.45 (above design flow coefficient) and ${\phi}$=0.60 (above design flow coefficient)] with and without GF. From the performance curves it is found that the performance of the fan improves considerably with GFs at lower Reynolds numbers and improves marginally at higher Reynolds number. Similar improvements are observed for the static pressures on the diffuser hub and shroud. The effect of Reynolds number on the performance and static pressures is considerable. However the effect is reduced with GFs.

KABUKI SYNDROME WITH PHONETIC & DENTAL PROBLEM: A CASE REPORT (구강내 이상소견과 언어 장애를 보이는 Kabuki 증후군환자의 증례보고)

  • Lee, Jong-Seok;Ko, Seung-O;Leem, Dae-Ho;Baek, Jin-A;Shin, Hyo-Keun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.6
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    • pp.681-683
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    • 2007
  • Kabuki(Niikawa-Kuroki) syndrome was first reported by Niikawa et al(1981). The faces of the patients are similar to the make-up of traditional Japanese Kabuki actors: long palpebral fissures, an ectropium of the lateral third of the eyelids, and arching eyebrows with sparse lateral halves. Craniofacial findings include a depressed nasal tip, short nasal septum, large and prominent ears, and micrognathia. Other main features area mild to moderate mental deficiency, short stature, skeletal and dermatoglyphic abnormalities, including prominent finger tip pads. Oral anomalies are common in KS(over 60%) and include abnormal dentition, widely spaced teeth, cleft palate or lip, high vault of palate, hypodontia, conical incisors, screw driver-shaped incisors and ectopic upper 6-year molars. The increased occurrence of cleft lip and palate or the development of a high vault of palate has been described by a number of authors. This condition is believed to be common in Japan, but has been reported from other parts of the world. The objective of this presentation is to report a case of this syndrome in six-year-old girl, with characteristic findings.

The Optimization of Fuel Injection Nozzles for the Reduction of NOx Emissions in a Large Diesel Engine (대형 디젤엔진의 NOx 저감을 위한 연료분사노즐 최적화 연구)

  • Yoon, Wook-Hyeon;Kim, Byung-Seok;Kim, Dong-Hun;Kim, Ki-Doo;Ha, Ji-Soo
    • Transactions of the Korean Society of Automotive Engineers
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    • v.12 no.6
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    • pp.60-65
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    • 2004
  • Numerical simulations and experiments have been carried out to investigate the effect of fuel injection nozzles on the combustion and NOx formation processes in a medium-speed marine diesel engine. Spray visualization experiment was performed in the constant-volume high-pressure chamber to verify the numerical results on the spray characteristics such as spray angle and spray tip penetration. Time-resolved spray behaviors were captured by high-speed digital camera and analyzed to extract the information on the spray parameters. Spray and combustion phenomena were examined numerically using FIRE code. Wave breakup and Zeldovich models were adopted to describe the atomization characteristics and NOx formation processes. Numerical results were verified with experimental data such as cylinder pressure, heat release rate and NOx emission. Finally, the effects of fuel injection nozzles on the engine performance were investigated numerically to find the optimum nozzle parameters such as fuel injection angle, nozzle hole diameter and number of nozzle holes. From this study, the optimum fuel injection nozzle (nozzle hole diameter, 0.32 mm, number of nozzle holes, 8 and fuel injection angle, $148^{\circ}$) was selected to reduce both the fuel consumption and NOx emission. The reason for this selection could be explained from the highest fuel-air mixing in the early phase of injection due to the longest spray tip penetration and the highest heat release rate after $19^{\circ}$ ATDC due to the increased injection duration.

3-D Inverse Dynamics Analysis of the Effect of Maximum Muscle Force Capacities on a Musculoskeletal System

  • Han, Kap-Soo;Kim, Kyungho
    • Journal of Electrical Engineering and Technology
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    • v.9 no.5
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    • pp.1774-1779
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    • 2014
  • It is known that muscle strength of human body can alter or deteriorate as aging. In this study, we present an inverse dynamics simulation to investigate the effect of muscle strength on performing the daily activities. A 3D musculoskeletal model developed in this study includes several segments of whole body, long and short muscles, ligaments and disc stiffness. Five daily activities such as standing, flexion, finger tip to floor, standing lift close and lifting flexed were simulated with varying the maximum muscle force capacities (MFC) of each muscle fascicles from 30 to $90N/cm^2$ with an increment of $30N/cm^2$. In the result, no solution can be obtained for finger tip to floor and lifting flexed with $30N/cm^2$. Even though the solution was available for standing lift close activity in case of $30N/cm^2$ capacity, many of muscle fascicles hit the upper bound of muscle strength which means that it is not physiologically possible to perform the acvities in reality. For lifing flexed, even the case of $60N/cm^2$ capaciy, represents the moderate healthy people, was not able to find the solutions, showing that 18 muscles among 258 muscle fascicles reached 100% of muscle capacity. The estimated results imply that people who have low muscle strength such as elders or rehabilitation patients were required higher muscle work to perform and maintain the same daily activities than healthy one.

Structure Design and Experimental Appraisal of the Drag Force Type Vertical Axis Wind Turbine (수직축 항력식 풍력터빈의 구조설계 및 실험평가)

  • Kim Dong-Keon;Keum Jong-Yoon;Yoon Soon-Hyun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.30 no.3 s.246
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    • pp.278-286
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    • 2006
  • Experiments were conducted to estimate the performance of drag force type vertical axis wind turbine with an opening-shutting rotor. It was operated by the difference in drag force generated on both sides of the blades. The rotational speed was measured by a tachometer in a wind tunnel and the tunnel wind speed was measured by using a pilot-static tube and a micro manometer. The performance test for a prototype was accomplished by calculating power, power coefficient, torque coefficient from the measurement of torque and rpm by a dynamometer controller. Various design parameters, such as the number of blades(B), blade aspect ratio(W/R), angle of blades$(\alpha)$ and drag coefficient acting on a blade, were considered for optimal conditions. At the experiment of miniature model, maximum efficiency was found at N=15, $\alpha=60^{\circ}$ and W/R=0.32. The measured test variables were power, torque, rotational speed, and wind speeds. The data presented are in the form of power and torque coefficients as a function of tip-speed ratio V/U. Maximum power was found in case of $\Omega=0.33$, when the power and torque coefficient were 0.14 and 0.37 respectively. Comparing model test with prototype test, similarity law by advance ratio for vertical axis wind turbine was confirmed.

Morphological and Hemodynamic Parameters for Middle Cerebral Artery Bifurcation Aneurysm Rupture Risk Assessment

  • Qin, Hao;Yang, Qixia;Zhuang, Qiang;Long, Jianwu;Yang, Fan;Zhang, Hongqi
    • Journal of Korean Neurosurgical Society
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    • v.60 no.5
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    • pp.504-510
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    • 2017
  • Objective : To investigate the morphological and hemodynamic parameters associated with middle cerebral artery (MCA) bifurcation aneurysm rupture. Methods : A retrospective study of 67 consecutive patients was carried out based on 3D digital subtraction angiography data. Morphological and hemodynamic parameters including aneurysm size parameters (dome width, height, and perpendicular height), longest dimension from the aneurysm neck to the dome tip, neck width, aneurysm area, aspect ratio, Longest dimension from the aneurysm neck to the dome tip (Dmax) to dome width, and height-width, Bottleneck factor, as well as wall shear stress (WSS), low WSS area (LSA), percentage of LSA (LSA%) and energy loss (EL) were estimated. Parameters between ruptured and un-ruptured groups were analyzed. Receiver operating characteristics were generated to check prediction performance of all significant variables. Results : Sixty-seven patients with MCA bifurcation aneurysm were included (31 unruptured, 36 ruptured). Dmax (p=0.008) was greater in ruptured group than that in un-ruptured group. D/W (p<0.001) and the percentage of the low WSS area ($0.09{\pm}0.13$ vs. $0.01{\pm}0.03$, p<0.001) were also greater in the ruptured group. Moreover, the EL in ruptured group was higher than that in unruptured group ($6.39{\pm}5.04$ vs. $1.53{\pm}0.86$, p<0.001). Multivariate regression analysis suggested D/W and EL were significant predictors of rupture of MCA bifurcation aneurysms. Correlation analyses revealed the D/W value was positively associated with the EL (R=0.442, p<0.01). Conclusion : D/W and EL might be the most two favorable factors to predict rupture risk of MCA bifurcation aneurysms.

Nasal alar rim redraping method to prevent alar retraction in rhinoplasty for Asian men: A retrospective case series

  • Choi, Jun Ho;Yoo, Hyokyung;Kim, Byung Jun
    • Archives of Plastic Surgery
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    • v.48 no.1
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    • pp.3-9
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    • 2021
  • Background For an attractive and natural tip contour in Asian rhinoplasty, insertion of a nasal implant and reinforcement of the cartilaginous framework are essential. However, scar contracture, which often results from augmentation with implant insertion and inadequate soft tissue coverage of the framework, is one of the most common causes of alar retraction. This study reports a novel method of redraping soft tissue along the alar rim to prevent alar retraction in Asians. Methods Twenty young Asian men who underwent primary rhinoplasty with septoplasty were retrospectively reviewed. After the usual rhinoplasty procedures, alar rim redraping was conducted for the soft tissue along the transcolumellar and bilateral infracartilaginous incisions. The longest axis of the nostril (a) and the height of the nostril from that axis (b) were measured in anterior-posterior and lateral views. The preoperative and postoperative ratios (b/a) were analyzed using the paired t-test. Results All 20 patients showed natural contours of the nasal tip, nostrils, and alae after a mean follow-up of 53.6 weeks (range, 52-60 weeks). The ratio of the nostril axes significantly decreased postoperatively in all patients except one, by an average of 11.08%±6.52% in the anterior-posterior view and 17.74%±8.49% in the lateral view (P<0.01). There were no complications, including asymmetry, contracture, subdermal plexus injury, flap congestion, or infection. Conclusions A quantitative analysis of alar retraction by evaluating the ratio of nostril axes showed that alar rim redraping is a simple and effective adjuvant technique for preventing alar retraction in rhinoplasty for young Asian men.

Dissolution Characteristics and Regenerated Miscanthus Sinensis Holocellulose Film Prepared by Dissolving the LiBr Solution (LiBr 수용액으로 용해시켜 제조한 거대억새 홀로셀룰로오스 용해 및 재생 필름특성)

  • Yang, Ji-Wook;Kwon, Gu-Joong;Hwang, Kyo-Jung;Hwang, Won-Jung;Hwang, Jae-Hyun;Kim, Dae-Young
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.47 no.6
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    • pp.89-97
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    • 2015
  • In this study, dissolution characteristics of 60% LiBr aqueous solution for Miscanthus sinensis holocellulose in accordance with heating time and characteristics of regenerated films were analyzed. Miscanthus sinensis holocellulose was made by peracetic acid method. During the dissolution of 60% LiBr solution for the holocellulose, the dissolution was started from the tip of the cellulose fiber after about 7 minutes, and proceeded as it swollen like a balloon. A lot of Si was identified by analyzing hollocellulose regenerated film through SEM/EDS. Cross section of regenerated film as dissolution time till 40 minutes of dissolution showed multilayered structure and fiber orientation. But after 40 minutes, multilayered structure and fiber orientation was not observed. The crystal structure of the holecellulose was transformed cellulose I into cellulose II. Therefore, dissolution for 20 minutes with 60% LiBr solution in the condition of $190^{\circ}C$ hot plate was shown as an optimum condition to manufacture the holocellulose regenerated film.

Comparison of the cyclic fatigue resistance of One Curve, F6 Skytaper, Protaper Next, and Hyflex CM endodontic files

  • Charlotte Gouedard;Laurent Pino;Reza Arbab-Chirani;Shabnam Arbab-Chirani;Valerie Chevalier
    • Restorative Dentistry and Endodontics
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    • v.47 no.2
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    • pp.16.1-16.9
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    • 2022
  • Objectives: This study compared the cyclic fatigue resistance of One Curve (C wire) and F6 Skytaper (conventional austenite nickel-titanium [NiTi]), and 2 instruments with thermos-mechanically treated NiTi: Protaper Next X2 (M wire) and Hyflex CM (CM wire). Materials and Methods: Ten new instruments of each group (size: 0.25 mm, 6% taper in the 3 mm tip region) were tested using a rotary bending machine with a 60° curvature angle and a 5 mm curvature radius, at room temperature. The number of cycles until fracture was recorded. The length of the fractured instruments was measured. The fracture surface of each fragment was examined with a scanning electron microscope (SEM). The data were analyzed using one-way analysis of variance and the post hoc Tukey test. The significance level was set at 0.05. Results: At 60°, One Curve, F6 Skytaper and Hyflex CM had significantly longer fatigue lives than Protaper Next X2 (p < 0.05). No statistically significant differences were found in the cyclic fatigue lives of One Curve, F6 Skytaper, and Hyflex CM (p > 0.05). SEM images of the fracture surfaces of the different instruments showed typical features of fatigue failure. Conclusions: Within the conditions of this study, at 60° and with a 5 mm curvature radius, the cyclic fatigue life of One Curve was not significantly different from those of F6 Skytaper and Hyflex CM. The cyclic fatigue lives of these 3 instruments were statistically significantly longer than that of Protaper Next.