• Title/Summary/Keyword: TFD

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Effects of Tea Fungus/Kombucha Beverage on Serum Protein Levels and Enzyme Activity in Streptozotocin-Induced Diabetic Rats (발효홍차가 당뇨쥐의 혈청 단백질농도 및 효소활성에 미치는 영향)

  • 고진복;최미애;김재영;노미희;김대진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1137-1143
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    • 1999
  • This study investigated the effect of tea fungus/kombucha beverage(TF) protein concentrations and enzyme activities in serum of both normal and diabetic male rats. Sprague Dawley growing rats were randomly assigned to one control and five diabetic groups. In five diabetic groups, D control group was fed drinking water and the other groups were fed drinking water supplemented with 20 or 40% TF (20 or 40% TFD group, respectively) and 20 or 40% disinfected TF(20 or 40% TFSD group, respectively) for 7 weeks. Diabetes was experimentally induced in all five diabetic groups by streptozotocin injection after 3 week feeding. The diabetic groups were significantly decreased the body weight( 29.4~ 48.6g) compared with those in control group(72.4g). The total liver and kidney weights in all diabetic groups were similar to those in control group, but those relative to body weights in all diabetic groups were heavier than those in control group. The total spleen weight in all diabetic groups was significantly decreased compared with those in control group, but those relative to body weights in all diabetic groups were similar to those in control group. The blood glucose levels were heigher in all diabetic groups than those in control group. The alkaline phosphatase activity in serum was higher in all experimental groups than those in control group, but it was lower in 40% TFD, 20% and 40% TFSD groups than those in D control group. The GPT activity was significantly increased in D control, 20% and 40% TFD groups than in control group. The GOT activity was significantly increased in D control goup than in control group, but those in all TFD and TFSD groups were similar to control group. The total protein concentration in all diabetic groups was significantly decreased compared with that in control group, but the albumin concentration showed almost the same levels in all the experimental groups. The ratio of albumin/globulin, and hem atocrit value were significantly increased in all diabetic groups than in control group. These results show that tea fungus/kombucha beverage with which diabetic rats were fed has not recovered the decreased body weight, lowered serum total protein level, hypertrophy of liver and kidney, hyperglycemia to the normal state.

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A Novel Picometer Positioning System for Machine Tools and Measuring Machines

  • Mizumoto, Hiroshi;Yabuta, Yoshito;Arii, Shiroh;Tazoe, Yoichi;Kami, Yoshihiro
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.123-128
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    • 2005
  • A novel tri-mode ultraprecision positioning system for machine tools and measuring machine is proposed. The basic coarse mode uses a Twist-roller Friction Drive (abbr. TFD), and controls several tens of millimeters of the machine-table travel with nanometer order of positioning resolution. The fine mode also utilizes the TFD with a fine adjusting mechanism. The resolution of the fine mode is in the range of sub-nanometer. For realizing picometer positioning, the ultra-fine mode is executed by using an active aerostatic guideway. On the bearing surface of this active guideway, several Active Inherent Restrictors (abbr. AIRs) are embedded for controlling the table position. An AIR unit consists of a piezoelectric actuator having a through hole, one end of the hole on the bearing surface acts as an inherent restrictor. Owing to the aerostatic mechanism of the AIR, the deformation of the piezoelectric actuator in the AIR unit causes much reduced table displacement. Such motion reduction is effective for ultraprecision positioning. Current positioning resolution of the ultra-fine mode is 50pm, however the final goal of the positioning resolution is expected to be in the order of picometer.

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Isolation and Characterization of 4-(2,4-Dichlorophenoxy)Butyric Acid-Degrading Bacteria from Agricultural Soils

  • Park, In-Hyun;Ka, Jong-Ok
    • Journal of Microbiology and Biotechnology
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    • v.13 no.2
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    • pp.243-250
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    • 2003
  • Eight numerically dominant 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB)-degrading bacteria and three pairs of bacteria showing syntrophic metabolism of 2,4-DB were isolated from soils, and their phylogenetic and phenotypic characteristics were investigated. The isolates were able to utilize 2,4-DB as a sole source of carbon and energy, and their 2.4-DB degradative enzymes were induced by the presence of 2.4-DB. Analysis of 16S rDNA sequences indicated that the isolates were related to members of the genera, Variovorax, Sphingomonas, Bradyrhizobium, and Pseudomonas. The chromosomal DNA patterns of the isolates obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences were distinct from each other. Four of the isolates had plasmids, but only one strain, DB 1, rad a transmissible 2,4-D degradative plasmid. When analyzed with PCR using primers targeted to the tfdA, B, and C genes, only strains DB2 and DB9a produced DNA bands of the expected sizes with the tfdA and C primers, respectively. All of the isolates were able to degrade 2,4-D as well as 2,4-DB, suggesting that the degradation pathways of these compounds were closely related to each other, but respiratory activities of many isolates adapted to 2,4-DB metabolism were quite low with 2,4-D.

Time-frequency Analysis of Vibroarthrographic Signals for Non-invasive Diagnosis of Articular Pathology (비침습적 관절질환 진단을 위한 관절음의 시주파수 분석)

  • Kim, Keo-Sik;Song, Chul-Gyu;Seo, Jeong-Hwan
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.4
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    • pp.729-734
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    • 2008
  • Vibroarthrographic(VAG) signals, emitted by human knee joints, are non-stationary and multi-component in nature and time-frequency distributions(TFD) provide powerful means to analyze such signals. The objective of this paper is to classify VAG signals, generated during joint movement, into two groups(normal and patient group) using the characteristic parameters extracted by time-frequency transform, and to evaluate the classification accuracy. Noise within TFD was reduced by singular value decomposition and back-propagation neural network(BPNN) was used for classifying VAG signals. The characteristic parameters consist of the energy parameter, energy spread parameter, frequency parameter, frequency spread parameter by Wigner-Ville distribution and the amplitude of frequency distribution, the mean and the median frequency by fast Fourier transform. Totally 1408 segments(normal 1031, patient 377) were used for training and evaluating BPNN. As a result, the average value of the classification accuracy was 92.3(standard deviation ${\pm}0.9$)%. The proposed method was independent of clinical information, and showed good potential for non-invasive diagnosis and monitoring of joint disorders such as osteoarthritis and chondromalacia patella.

Effect of Tea Fungus/Kombucha Beverage on Lipid Metabolism in Streptozotocin-induced Diabetic Male Rats (발효홍차가 당뇨숫쥐의 지질 대사에 미치는 영향)

  • 고진복;최미애
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.3
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    • pp.613-618
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    • 1999
  • This study investigated the effect of tea fungus/kombucha(TF) beverage on body weights, pancreas weights, serum glucose and lipid concentrations of both normal and diabetic male rats. Sprague Dawley male rats weighing $140{\pm}10g$ were randomly assigned to one control and five streptozotocin(STZ) diabetic groups. Diabetic groups were divided into D control(TF free water), 20% TFD(20% TF in water), 40% TFD(40% TF in water), 20% TFSD(20% TF disinfection in water) and 40% TFSD(40% TF disinfection in water) according to the level of TF supplementation. Diabetes was experimentally induced by intraperitoneally administration of STZ in citrate buffer(pH4.3) after 3 week feeding of four experimental water. Animals were sacrificed at the 4 week of diabetic state. The diabetic groups showed significant decrease of body weight(-29.4~48.6g) compared with the control group(72.4g). Pancreatic weights relative to body weights in all diabetic groups were heavier than those of the control group. The levels of fasting serum glucose were higher in all diabetic groups than that of the control group. The concentrations of triglyceride in serum was lower in 20% TFD and 20% TFSD groups than in those D control group. The levels of total cholesterol, LDL cholesterol in serum and atherogenic index were significantly decreased in all(20 or 40%) TFD and TFSD groups than in those D control group, but those were similar to control group. The levels of HDL cholesterol in serum was similar to all groups, but HDL cholesterol/total cholesterol ratio were significantly increased in all(20 or 40%) TFD and TFSD groups than in those D control group. Liver triglyceride concentration of the all diabetic groups significantly decreased compared with the control group. Liver total cholesterol and phospholipid were not affected by TF and TFS feeding. The results indicated that tea fungus/kombucha beverage feeding improved the cholesterol, triglyceride and atherogenic index levels in diabetic rats.

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Application of DNA Probe Method for Detection of 2,4-Dichlorophenoxyacetic Acid Degrading Bacteria in Soil (DNA Probes에 의한 토양의 이사디 (2,4-D) 분해세균의 검출)

  • Ka, Jong-Ok
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.403-408
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    • 1996
  • Total bacterial community DNA, which was extracted from microcosm soil and field soil after 2,4-D amendments, was analyzed on Southern blots, using the tfdA gene probe derived from plasmid pJP4 and the Spa probe from Sphingomonas paucimobilis. Southern blot analyses with total bacterial DNA extracted from soils Inoculated with Pseudomonas cepacia/pJP4 revealed that DNA probe method could detect the 2,4-D degrading bacteria down to $10^5\;cells/g$ dry soil. In the microcosm experiment, there was a good correlation between 2,4-D degradation and banding patterns in hybridization analyses performed after each 2,4-D treatment using the two probes. When bacterial DNA extracted from microcosm soil was hybridized with the Spa probe, a change in the position of hybrid bands was observed over time in a Southern blot, suggesting that population change or possibly genetic rearrangement in 2,4-D degrading microbial populations occurred in this soil. With the Spa probe, one hybrid DNA band was persistently observed throughout the five 2,4-D additions. When bacterial DNA isolated from the field soil was probed with the tfdA and Spa, strong hybridization signal was observed in the 100 ppm-treated subplot, weak signal In the 10 ppm-treated subplot, and no significant signal in the 1 ppm-treated and control subplots. The data show that DNA probe analyses were capable of detecting and discriminating the indigenous 2,4-D degrading microbial populations in soil amended with 2,4-D under laboratory and field conditions.

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Prediction of Defibrillation Success of Ventricular Fibrillation ECG Signals using Time-Frequency Analysis (시-주파수 분석을 이용한 심실세동시 심전도 분석을 통한 제세동 예측에 관한 연구)

  • Sung, Hong-Mo;Shin, Jae-Woo;Lee, Hyun-Sook;Hwang, Sung-Ho;Yoon, Young-Ro
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.55 no.4
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    • pp.181-188
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    • 2006
  • The purpose of this study is to predict the defibrillation success of a ventricular Fibrillation ECG signal using time-frequency analysis. During CPR, coronary perfusion pressure and electrocardiogram were measured. Parameters extracted from time-frequency domain were served as predictor of resuscitation success. Time frequency distribution(TFD) of ECG signals was estimated from the smoothed pseudo Wigner-Ville distribution(SPWVD). Median frequency, peak frequency, 1/f slope, frequency band ratios$(2{\sim}4Hz,\;4{\sim}6Hz,\;6{\sim}8Hz,\;8{\sim}10Hz,\;10{\sim}12Hz,\;12{\sim}15Hz)$ were extracted from each TFD as function of time. Paired t-test was used to determine the differences in ROSC and non-ROSC groups. In the statistical results, we selected four significant parameters - median frequency, 1/f slope, $2{\sim}4Hz$ band ratio, $8{\sim}10Hz$ band ratio. We made an attempt to predict defibrillation success by combining features extracted from time frequency distribution. Independent t-test was used to determine the differences ROSC and non-ROSC groups. Consequently, we selected four significant parameters-median frequency, 1/f slope, $2{\sim}4Hz$ band ratio, $8{\sim}10Hz$ band ratio. The relationship between coronary perfusion pressure and ECG parameters was analyzed with linear regression analysis. R-square value was 55%. 1/f slope and $8{\sim}10Hz$ band ratio had the significant relationship with coronary perfusion pressure.

Analysis of Effects of Rational and Emotional Advertising Appeals on Products from a View of Psychological Distance : Focusing on the Eye-Tracking Method (제품에 대한 심리적 거리감에 따른 이성적, 감성적 광고소구의 효과분석: 아이트래킹 방법을 중심으로)

  • Kang, Chang Min;Lee, Kun Chang
    • Journal of Digital Convergence
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    • v.17 no.11
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    • pp.97-104
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    • 2019
  • Consumers possess their own psychological distance towards products. Based on the construal-level theory, this study aims to analyze which type of advertising appeal is more effective according to psychological distance. Also this study conducts neurophysiology experiment through eye-tracker. By using eye-tracking methods, participants' responses to products was measured and analyzed accordingly. Results revealed that consumers prefer rational appeals when the psychological distance is perceived near, while emotional appeals are preferred when the psychological distance is perceived far. In addition, eye movements measured in Visit Count(VC), Fixation Count(FC), and Total Fixation Duration(TFD) showed significant difference when participants are viewing preferred and non-preferred appeals.

Sub-Nyquist Nonuniform Sampling and Perfect Reconstruction of Speech Signals (음성신호의 Sub-Nyquist 비균일 표준화 및 완전 복구에 관한 연구)

  • Lee, He-Young
    • Speech Sciences
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    • v.12 no.2
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    • pp.153-170
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    • 2005
  • The sub-Nyquist nonuniform sampling (SNNS) and the perfect reconstruction (PR) formula are proposed for the development of a systematic method to obtain minimal representation of a speech signal. In the proposed method, the instantaneous sampling frequency (ISF) varies, depending on the least upper boundary of spectral support of a speech signal in time-frequency domain (TFD). The definition of the instantaneous bandwidth (IB), which determines the ISF and is used for generating the set of samples that represent continuous-time signals perfectly, is given. Also, the spectral characteristics of the sampled data generated by the sub-Nyquist nonuniform sampling method is analyzed. The proposed method doesn't generate the redundant samples due to the time-varying property of the instantaneous bandwidth of a speech signal.

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