• 제목/요약/키워드: TEIN

검색결과 31건 처리시간 0.028초

Novel $Ca^{2+}$-ATPase Found in the Human Milk Membrane Fraction

  • Cho, Jin-Kook;Kanno, Choemon
    • 한국유가공학회:학술대회논문집
    • /
    • 한국유가공기술과학회 1997년도 춘계 제44회 유가공 심포지움
    • /
    • pp.23-34
    • /
    • 1997
  • Calcium-stimulated ATPase ($Ca^{2+}$-ATPase) which has optimal pH value at 7.0 was found in the membrane fraction of human milk, and its enzymatic properties were studied. The purified $Ca^{2+}$-ATPase required 0.45 mM Ca ion for maximal activity. Among the nucleosides, $Ca^{2+}$-ATPase showed a higher substrate specificity to ATP and UTP than to CTP and GTP. $Ca^{2+}$-ATPase had apparent Km value of 0.065, and V max of 7.63 mol ATP hydrolyzed/mg pro-tein per min, respectively. $Ca^{2+}$-ATPase was potently inhibited by lanthanide, vanadate, and p-chloromercuribenzoate, and inactivated by EDTA, and CDTA and EGTA, but were unaffected by N-ethylmaleimide, $NaN_3$, ouabain, or oligomycin, and was completely inactivated by heating at $60^{\circ}C$ for 10 min. This enzyme activity was concentrated in the membrane fraction of the cream and skim milk membrane, but not founded in bovine milk.

  • PDF

Porous Materials Based on Nickel and Aluminum

  • Dubinina, L.V.;Lopatin, V.U.;Narva, V.K.;Shugaev, V.A.;Vin, Tein
    • 한국분말야금학회:학술대회논문집
    • /
    • 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part 1
    • /
    • pp.614-615
    • /
    • 2006
  • Investigation of influence the morphology of initial powder particles, application pore-formers for sintering of nickel powders and application of flux for sintering of aluminum was made. Using different methods was prepared material with size of porous in wide range size of pores ($1-500{\mu}m$). Using the flux for gravity sintering of aluminum in air atmosphere was manufactured porous material with porosity about 45%..

  • PDF

Selection of Optimum Expression System for Production of Kringle Fragment of Human Apolipoprotein(a) in Saccharomyces cerevisiae

  • Cha Kwang Hyun;Kim Myoung Dong;Lee Tae Hee;Lim Hyung Kweon;Jung Kyung Hwan;Seo Jin Ho
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제9권6호
    • /
    • pp.523-527
    • /
    • 2004
  • Recombinant Saccharomyces cerevisiae expression systems were developed to pro­duce a novel human anti-angiogenic protein called LK8, an 86 amino-acid kringle fragment pro­tein with three disulfide linkages. Galactose-inducible LK8 expression plasmid was constructed, and LK8 production levels by four S. cerevisiae strains were compared in order to select an op­timal host strain. S. cerevisiae 2805 was the most efficient among the strains tested. Elevating the LK8 gene copy number through multiple integration using 8-sequences as target sites re­sulted in more than a two-fold increase in the LK8 production level compared with the plasmid­based expression system. The maximum LK8 protein concentration of 25 mg/L was obtained from batch cultivation of the yeast transformant that harbors 16 copies of the LK8 gene. In con­clusion, the strain integrated with the multiple LK8 gene secreted the protein with relatively high yield, although, the increased LK8 gene dosage over 11 copies did not lead to further en­hancement in batch cultivations.

백미에 쑥 첨가급식이 이유식후 백서의 영향에 미치는 영향 (A Weanling Rats by Feeling Mugwort Powder Supplemented to Rice Diets with Different ProStudy on the Nutritional Effect in tein Level)

  • 황호형
    • Journal of Nutrition and Health
    • /
    • 제19권1호
    • /
    • pp.16-22
    • /
    • 1986
  • This study was designed to investigate the nutritional effect of mugowrt powder supple mentation to rice diets with different protein levels. Thirty female albino rats weighing 39-43g were adopted for the feeding trial for 4 weeks. The different 5 kind of experimental diets were performed . Control diet was commercially available forage for rats, experimental diet (I-C) highly milled rice, (Ⅰ) highly milled rice 95% and mugwort powder 5%, (Ⅱ-C)highly milled rice 95% and milk casein 5%, and (Ⅱ) highly milled rice 90%, milk casein 5 % and mugwort powder 5%. Growth rate was remarkably high in the dietary group fed on highly milled rice supplemented with 5% mugwort powder (protein 8%) (P<0.05), but it showed the tendency to be rather low in the group fed on highly milled rice supplemented with 5% casein and 5% mugwort powder (protein 12%). Food efficiency as well as protein efficiency appeared similar to the growth rate. Hematodcrit level demonstrated the same tendency as growth rate, but hemoglobin content was observed to increase by diets supplemented with increasing amount of mugwort regardless of protein level. Each nutrient intake was increased by adding mugwort powder to diets after a week's feeding, but it was increased by 8% protein diet, and decreased by 12% protein diet in 3 weeks as well as 4 weeks after feeding . The absorption rate of carbohydrate and protein decreased by feeding mugwort supplemented diets regardless of protein level and feeding period, and that of lipid increased with 12% protein diet.

  • PDF

수집종 자소의 주요 양적형질의 변이와 화학성분 함량 (Variation of Quantitative Charactes and Chemical Components on Local Collections of Perilla frutescens)

  • 박희진
    • 한국자원식물학회지
    • /
    • 제7권2호
    • /
    • pp.109-114
    • /
    • 1994
  • Followings are resulted through the analysis of the maln characters and the leaf ingredients, which is on the purpose of getting the basic materials to improve jaso and increase its production.Of the introduced varieties, Jukyeubjaso-stem length is 180cm, ear length is 37cm, No. of branches ofstem is 27, leaf length Is 16cm, leaf width Is 15cm-has better character than any other. Gurye local-stem length is 179cm, ear length is 38cm-is taken to be the best in the Geo-ecotypic.In the yield of leaf and seed, JukyeubJaso Is 727kg/10a, the greatest of all. In the leaf softness, allthe Inirorluced varieties are about 3.5 degrees, the Geo-ecotypic, about 2. The qualify of the introducedvat'ieties is better· than that of the Geo-ecotypic. Estimating on the chemical analysis of leaf,Jukyeubjaso has Protein 21.84%, Total carbohydrate 7.91%, Fe 126ppm, and Gurye lacal has Protein21.7 a, Total carbohydrate 8.4%, Fe 148ppm, expressing the highest. In correlation coefficients of leafchemical components, between protein and Total carbohydrate, Protein and Mn, protein and Fe, Pro-tein and Cu all the sorts show the positive correlation. Among them the Geo-ccoyplcs show the hlghestsignificance.

  • PDF

A Novel Bandwidth Estimation Method Based on MACD for DASH

  • Vu, Van-Huy;Mashal, Ibrahim;Chung, Tein-Yaw
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • 제11권3호
    • /
    • pp.1441-1461
    • /
    • 2017
  • Nowadays, Dynamic Adaptive Streaming over HTTP (DASH) has become very popular in streaming multimedia contents. In DASH, a client estimates current network bandwidth and then determines an appropriate video quality with bitrate matching the estimated bandwidth. Thus, estimating accurately the available bandwidth is a significant premise in the quality of video streaming, especially when network traffic fluctuates substantially. To cope with this challenge, researchers have presented various filters to estimate network bandwidth adaptively. However, experiment results show that current schemes either adapt slowly to network changes or adapt fast but are very sensitive to delay jitter and produce sharply changed estimation. This paper presents a novel bandwidth estimation scheme based on Moving Average Convergence Divergence (MACD). We applied an MACD indicator and its two thresholds to classifying network states into stable state and agile state, based on the network state different filters are applied to estimate network bandwidth. In the paper, we studied the performance of various MACD indicators and the threshold values on bandwidth estimation. Then we used a DASH proxy-based environment to compare the performance of the presented scheme with current well-known schemes. The simulation results illustrate that the MACD-based bandwidth estimation scheme performs superior to existing schemes both in the speed of adaptively to network changes and in stability in bandwidth estimation.

The Performance and Evaluation for Recycling of Waste Glass

  • Chang, Tein-Chin;Huang, Jian-Er;Yen, Jia-Huei
    • 대한전자공학회:학술대회논문집
    • /
    • 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
    • /
    • pp.80-83
    • /
    • 2001
  • According to the EPA in Taiwan report, 9.05 million metric tons of solid wastes were generated in 1999, and the waste glass was accounted of 4.95 percent. However, with the increasing tonnage of disposal cost and existing disposal sites are reaching full capacity, recycling is currently accepted as a sustainable approach to waste management. Therefore, it's essential and urgent that the government in Taiwan establish the recycling and recovery framework for the minimization of the solid waste, reduction of materials and energy consumption, and the encouragement for the reuse, recycle and recovery development. To achieve this Boal, Taiwan has been strived for a long period of time in waste glass recovery and recycle. Waste glass, unlike other kinds of resource waste, is 100% recyclable. The EPA in Taiwan now center on a lot of different kinds of waste glass, such as glass container, flat glass, CRT glass, windshields glass, fluorescent lamps, and waste pesticide glass container. This article will focus on the framework of the recycling market access, and also try to provide some strategies to improve waste glass recycling efficiently.

  • PDF

Change in Levels of Endogenous Hormone and Detection of Adventitious Bud-Related Protein during Culture of Hybrid Poplar Explants

  • Song, Jae-Jin
    • Journal of Plant Biology
    • /
    • 제38권2호
    • /
    • pp.143-151
    • /
    • 1995
  • Addition of plant growth hormones [0.01 mg/L NAA and 0.2mg/L benzyladenine (BA)] to a woody plant medium stimulated the adventitious bud formation of poplar explants during culture. Endogenous IAA content increased rapidly at the initial culture stage and then decreased, being followed by rapid increment again at the late culture. But the content of trans-zeatin riboside (t-ZR) increased continuously during the culture. Cytoplasmic soluble proteins were analyzed by one- and two-dimensional SDS-PAGE. Increased amount of 40 kD band was detected by one-dimensional electrophoresis using Coomassie Blue staining during the culture and two distinctive proteins whose mol wt is 40,000 were detected by two-dimensional electrophoresis using autoradiography and these proteins were synthesized continuously prior to the adventitious bud formation. When the midvein segments were transferred to the actinomycin D-containing medium, the spots of adventitious bud-related proteins(ABRPs) did not disappeared but weakened in intensity. So, it is concluded that genes coding for the ABRPs are regulated to some degree at the transcriptional level. Also, they were not observed in BA-free medium, suggesting that these proteins be regulated by cytokinin, which made then possible to form the adventitious bud.

  • PDF

Identifiaction and Molecular Size of Zine-Binding Ligands in Pancreatic/Biliary Fluid of Rats

  • Kwun, In-Sook;Donald Oberleas
    • Preventive Nutrition and Food Science
    • /
    • 제2권1호
    • /
    • pp.42-48
    • /
    • 1997
  • the exocrine pancreatic secretion is an important factor in the maintenance of zinc homeostasis. The daily pancreatic secretion of zinc into the gastrointestinal tract may be two or more times the daily dietary zinc intake. The objective of this study was to examine the distribution of proteins and zinc in pancreatic/biliary fluid following intraperitoneal {TEX}${65}^Zn${/TEX} injection into dietary prepared Sprague-Dawly rats. Distribution of zinc-binding protein in Sephadex G-75 subfractions showed a peak corresponding to the high molecular weight protein standard(<66kDa) in the pancreatic/biliary fluid. Zinc also was associated with the 29~35kDa mole-cular weight proteins. These are similar in size with zinc-containing enzymes, carboxypeptidase A and car-boxypeptidase B. A more remarkable small molecular weight fraction eluted beyond the 6.5kDa standard pro-tein peak. These results show the presence of small molecular weight compound in pancreatic/biliary fluid associated with zinc . These small molecular weight compounds may serve as zinc-binding ligands for the secretion of enogenous zinc into the duodenum. These findings suggest that these lignads may dissociate zinc in the duodenum thus making it vulnerable to complexation with phytate in the upper gastrointestinal tract rendering the zinc unavailable for reabsorption.

  • PDF