• Molecules and Cells
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• v.44 no.3
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• pp.179-185
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• 2021

Vancomycin response regulator (VncR) is a pneumococcal response regulator of the VncRS two-component signal transduction system (TCS) of Streptococcus pneumoniae. VncRS regulates bacterial autolysis and vancomycin resistance. VncR contains two different functional domains, the N-terminal receiver domain and C-terminal effector domain. Here, we investigated VncR C-terminal DNA binding domain (VncRc) structure using a crystallization approach. Crystallization was performed using the micro-batch method. The crystals diffracted to a 1.964 Å resolution and belonged to space group P212121. The crystal unit-cell parameters were a = 25.71 Å, b = 52.97 Å, and c = 60.61 Å. The structure of VncRc had a helix-turn-helix motif highly similar to the response regulator PhoB of Escherichia coli. In isothermal titration calorimetry and size exclusion chromatography results, VncR formed a complex with VncS, a sensor histidine kinase of pneumococcal TCS. Determination of VncR structure will provide insight into the mechanism by how VncR binds to target genes.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.1025-1028
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• 2009

고속도로의 다양한 주행특성으로는 과속하는 차량, 휴게소나 기타목적의 이용차량, 운전자의 습관이나 피로도등이 있는데 이에 따라 고속도로 주행시간에 차이가 나타난다. 하지만 현재에는 이러한 특성을 고려하지 않고 통행시간 분류가 되고 있어 정확성과 신뢰성을 보장하지 못하고 있는 실정이다. 이에 본 연구에서는 데이터 분포에 따른 해석을 통하여 TCS데이터의 특성을 고려 할 수 있는 Fuzzy c-means 알고리즘과 단순히 임의의 초기값으로 분류하는 K-means와의 비교를 통해서 주행특성을 고려한 클러스터링 기법이 경우에 따라서 더 효과적이고 신뢰성 있는 분류방법이 될 수 있음을 증명하였다.

• 한국ITS학회:학술대회논문집
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• 2008.11a
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• pp.455-462
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• 2008

• Journal of Environmental Science International
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• v.18 no.5
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• pp.539-550
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• 2009

This study found that tropical cyclones (TCs) formed for fall in 2007 over the western North Pacific were distributed in high-latitudes comparing to 56-year (1951-2006) climatological mean. The frequency and latitude of TC genesis became higher than 56-year climatological mean from September onward in 2007 and all the TCs that formed to the north of 20$^{\circ}$N was also distributed after September in 2007. These characteristics of TC genesis for fall in 2007 could be confirmed through analyzing various variables, such as a large-scale atmospheric circulation, outgoing longwave radiation (OLR), vertical zonal wind shear, and sea surface temperature (SST). On the other hand, a frequency of the TC that occurred to the north of 200N showed a clear interdecadal variation and its decreasing trend was distinctive in recent years. Its intensity was also weaker that TCs that did to the south of 20$^{\circ}$N. However, a latitude of TC genesis showed an increasing trend until recent years, whose variation was consistent with trend that through a SST analysis, warm SST went north in recent years.

• Korean Journal of Environmental Agriculture
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• v.30 no.1
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• pp.82-88
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• 2011

BACKGROUND: Tetracyclines (TCs) are mainly regulated as parent compounds by bioactivity-based screening methods in food. Especially with respect to antimicrobial residues, their metabolites/epimers are also highly concerning chemicals and traditionally applied microbial detection methods are needed to improve with validation for regulatory control. METHODS AND RESULTS: Detection capability and biological activity of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and their epimers; anhydrotetracycline (ATC), epianhydrotetracycline (EATC), epitetracycline (ETC), 4-epi-chlortetracycline (ECTC), 4-epianydrochlotetra-cycline (EACTC), 4-epioxychlortetracycline (EOTC), were measured by microbial growth inhibition screening method of Korea Food Code. CONCLUSION(S): Limited detection capabilities were found, B. megarerium and B. subtilis showed for TC and CTC, and B. subtilis for OTC. Biological potency of each epimer was also presented against various microorganisms, at the level from 50% to 96%, comparing with parent TCs. It is recommended that more advanced microbial screening methods with validation are needed, and biologically active epimers are to be considered as marker residues for MRL setting of regulatory control purpose.

• Atmosphere
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• v.20 no.4
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• pp.451-466
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• 2010

This edition has continued since 2006 tropical cyclone season our effort to provide standard tropical cyclone summaries by the western North Pacific basin and detailed reviews of operationally or meteorologically significant tropical cyclones to document significant challenges and shortfalls in the tropical cyclone warning system to serve as a focal point for research and development efforts. The tropical cyclone season of 2009 in the western North Pacific basin is summarized and the main characteristics of general atmospheric circulation are described. Also, the official track and intensity forecasts of these cyclones are verified. The total number is less than 59-year (1951~2009) average frequency of 26.4. The 2009 western North Pacific season was an inactive one, in which 22 tropical storms generated. Of these, 13 TCs reached typhoon (TY) intensity, while the rest 9 TCs only reached severe tropical storm (STS) and tropical storm (TS) intensity - three STS and six TS storms. On average of 22 TCs in 2009, the Korea Meteorological Administration official track forecast error for 48 hours was 219 km. There was a big challenge for individual cyclones such as 0902 CHAN-HOM, 0909 ETAU, and 0920 LUPIT resulting in significant forecast error, with both intricate tracks and irregular moving speed. There was no tropical cyclone causing significant direct impact to the country. The tropical cyclone season in 2009 began in May with the formation of KUJIRA (0901). In September and October, ten TSs formed in the western North Pacific in response to enhanced convective activity. On the other hand, the TC activity was very weak from June to July. It is found that the unusual anti-cyclonic circulation in the lower level and weak convection near the Philippines are dominant during summertime. The convection and atmospheric circulation in the western North Pacific contributed unfavorable condition for TC activity in the 2009 summertime. Year 2009 has continued the below normal condition since mid 1990s which is apparent in the decadal variability in TC activity.

• International Journal of Highway Engineering
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• v.16 no.5
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• pp.99-108
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• 2014

PURPOSES : The travel times of expressway buses have been estimated using the travel time data between entrance tollgates and exit tollgates, which are produced by the Toll Collections System (TCS). However, the travel time data from TCS has a few critical problems. For example, the travel time data include the travel times of trucks as well as those of buses. Therefore, the travel time estimation of expressway buses using TCS data may be implicitly and explicitly incorrect. The goal of this study is to improve the accuracy of the expressway bus travel time estimation using DSRC-based travel time by identifying the appropriate analysis period of input data. METHODS : All expressway buses are equipped with the Hi-Pass transponders so that the travel times of only expressway buses can be extracted now using DSRC. Thus, this study analyzed the operational characteristics as well as travel time patterns of the expressway buses operating between Seoul and Dajeon. And then, this study determined the most appropriate analysis period of input data for the expressway bus travel time estimation model in order to improve the accuracy of the model. RESULTS : As a result of feasibility analysis according to the analysis period, overall MAPE values were found to be similar. However, the MAPE values of the cases using similar volume patterns outperformed other cases. CONCLUSIONS : The best input period was that of the case which uses the travel time pattern of the days whose total expressway traffic volumes are similar to that of one day before the day during which the travel times of expressway buses must be estimated.

• Biomedical Science Letters
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• v.17 no.3
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• pp.197-202
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• 2011

Mitosis count is one of the most helpful morphologic features for distinguishing pulmonary typical carcinoid (TC) from atypical carcinoid (AC). However, identifying areas of highest mitotic activity is tedious and time-consuming, and mitosis count may vary substantially among pathologists. Anti-phosphohistone H3 (PHH3) is an antibody that specifically detects histone H3 only when phosphorylated at serine 10 or serine 28, an event that is concurrent with mitotic chromatin condensation and not observed during apoptosis. In this study, immunohistochemical staining for PHH3 was performed to determine whether PHH3 was a reliable and objective mitosis-specific marker for pulmonary carcinoid tumors. Seventeen cases of surgically resected pulmonary carcinoid tumors (12 TCs and 5 ACs) were obtained and classified according to the 2004 World Health Organization classification. Mitotic counts determined by PHH3 correlated to ones determined by hematoxylin and eosin (H&E) staining; however, PHH3 mitotic counts (mean mitotic counts: 1 in TCs and 3.2 in ACs) were slightly higher than H&E mitotic counts (mean mitotic counts: 0.25 in TCs and 1.8 in ACs). The mitotic counts determined by experienced observer were more correlated to those determined by inexperienced observer with the PHH3-based method (R=0.968, P<0.001) rather than H&E staining (R=0.658, P<0.001). These results suggest that the PHH3 mitotic counting method was more sensitive and simple for detecting mitoses compared to traditional H&E staining. Therefore, PHH3 immunohistochemistry may contribute to more accurate and reproducible diagnosis of pulmonary carcinoid tumors and may be a valuable aid for administrating appropriate clinical treatment.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.17 no.4
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• pp.41-53
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• 2018

This study developed the optimization method to correct the measured traffic volume of the expressway that minimizes the measurement error and satisfies the traffic balancing with TCS. For this purpose, the model constructed in this study was compared and verified with the true traffic volume. Verification result of the model, it was found that the measurement error is reduced when the measured traffic volume is corrected for the traffic volume balance. As a result of applying it to 40 links of the Kyoungbu expressway, the measured traffic volume was corrected by -8.1%~9.6% and the measurement error was decreased as much as the corrected traffic volume. This research is meaningful in improving the accuracy of the measured traffic volume of the expressway, while the scale and role of the expressway are increasing.

• Journal of the Korean Society of Propulsion Engineers
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• v.9 no.4
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• pp.96-103
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• 2005

The main purpose of launch vehicle is to insert satellite into a target orbit safely and correctly. To accomplish the main purpose of launch vehicle, the inserting velocity, inserting angle, and final mass of launch vehicle should be within the allowable range. In general, such requirements are satisfied with applying TCS(Thrust Control System) and TDS(Tank Depletion System), which manage thrust and mixture ratio by controlling propellant flow rate with thrust and mixture ratio control valves. In this study, the control characteristics of thrust and mixture ratio control valve were examined by PID control logic for stable operation of liquid-Propellant rocket engine at on-dosing point. The analysis on the control characteristics of control valves was done with AMESim code and the results from control valve test facility at KARI.