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http://dx.doi.org/10.5338/KJEA.2011.30.1.82

Epimers/Metabolites of Tetracycline Derivatives; Biological Activity and Regulation Aspects for MRL in Food  

Kwon, Jin-Wook (National Veterinary Research & Quarantine Service (NVRQS))
Yun, Hyo-In (College of Veterinary Medicine, Chungnam Nat'l Univ.)
Lee, Kyu-Seung (Department of Applied Biology & Chemistry, College of Agriculture & Life Science, Chungnam Nat'l Univ.)
Publication Information
Korean Journal of Environmental Agriculture / v.30, no.1, 2011 , pp. 82-88 More about this Journal
Abstract
BACKGROUND: Tetracyclines (TCs) are mainly regulated as parent compounds by bioactivity-based screening methods in food. Especially with respect to antimicrobial residues, their metabolites/epimers are also highly concerning chemicals and traditionally applied microbial detection methods are needed to improve with validation for regulatory control. METHODS AND RESULTS: Detection capability and biological activity of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and their epimers; anhydrotetracycline (ATC), epianhydrotetracycline (EATC), epitetracycline (ETC), 4-epi-chlortetracycline (ECTC), 4-epianydrochlotetra-cycline (EACTC), 4-epioxychlortetracycline (EOTC), were measured by microbial growth inhibition screening method of Korea Food Code. CONCLUSION(S): Limited detection capabilities were found, B. megarerium and B. subtilis showed for TC and CTC, and B. subtilis for OTC. Biological potency of each epimer was also presented against various microorganisms, at the level from 50% to 96%, comparing with parent TCs. It is recommended that more advanced microbial screening methods with validation are needed, and biologically active epimers are to be considered as marker residues for MRL setting of regulatory control purpose.
Keywords
Biological potency; Chlortetracycline; Epimer; Oxytetracycline; Tetracycline;
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