• 한국수정란이식학회지
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• 제32권4호
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• pp.311-317
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• 2017

Cryopreservation of oocytes by vitrification technique may contribute a lot in the field of reproductive biotechnology. The objectives of the present study were to evaluate the effectiveness of two cryo-devices for vitrification of immature oocytes of indigenous zebu cows. Slaughter house derived immature cumulus-oocyte-complexes (COCs) of cows were vitrified using 15% dimethyl sulphoxide (DMSO) as cryoprotective agent (CPA) with 0.5 mol sucrose in TCM 199 supplemented with 20% FBS. Vitrification of COCs was completed after immediate plunging of COCs loaded cryotop or French mini straw into the liquid nitrogen ($LN_2$). Then the COCs containing cryotop or French mini straws were warmed in 0.25 mol sucrose and 20% FBS supplemented TCM 199 followed by in vitro culture in $50{\mu}l$ droplets of bicarbonate buffered TCM 199 supplemented with 10% FBS, pyruvate, FSH and oestradiol for 24 hrs at $39^{\circ}C$ with 5% CO2 in humidified air. After maturation culture, oocytes were denuded and examined under inverted microscope for presence of polar body as the indication of maturation. Denuded oocytes were also stained by whole mount technique using 1% orcein to examine the maturation by presence of MII chromosomes. The in vitro maturation rate was significantly (p<0.05) higher in oocytes vitrified and warmed using crytop ($47.1{\pm}6.9%$) than that of French mini straw ($15.9{\pm}12.5%$). Moreover, in vitro maturation rate was significantly (p<0.05) highe r in control oocytes (not vitrified) ($84.5{\pm}14.2%$) than that of vitrified oocytes. In conclusion, cryotop is better than French mini straw as cryo-device for vitrification of bovine immature oocytes.

• 한국가축번식학회지
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• 제19권3호
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• pp.191-195
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• 1995

본 연구는 체외성숙한 소의 난포란을 ethanol로 활성화시켰을 때 cytochalasin에 노출한 시간과 농도가 난포란의 배반포단계로의 발달에 미치는 영향을 검토하였다. 30시간 체외성숙시킨 소 난포란을 7% ethanol이 든 Dulbecco's phosphate buffered saline에 7분간 노출시켜 활성화를 시킨 후, cytochalasin D가 첨가된 TCM＋199＋10% fetal calf serum에서 일정시간 배양하여 세포주기를 억제시켰다. 난포란을 활성화시킨 후, 0, 5, 10 그리고 15시간 cytochalasin D(5$\mu\textrm{g}$/ml)에 노출시켰을 때, 5시간에서 가장 높은 배반포(16%), 팽윤배반포(13%), 부화배반포(7%)로의 발달률을 보였다. 또한 cytochalasin D 0, 2.5, 5 그리고 7.5$\mu\textrm{g}$/ml에서 7시간 노출시켰을 때, 2.5$\mu\textrm{g}$/ml에서 가장 높은 배반포(13%), 팽윤배반포(7%), 부화배반포(4%)로의 발달률을 보였다. 결론적으로 cytochalasin D는 난폰란의 단위발생에 중요한 역할을 하며, 성숙한 난포란은 cytochalasin D 2.5$\mu\textrm{g}$/ml에서 5시간 노출하였을 때 가장 높은 배반포로의 발달률을 보여주었다.

• 동의생리병리학회지
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• 제24권5호
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• pp.758-769
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• 2010

This is a preliminary study for examining the identity issue of the current Traditional Korean Medicine(TKM). In order to examine the identity issue, it is necessary to understand "what" current TKM is. The current TKM has been formed in the complicated historical settings and the newly formed academic geography of modern times, completely different from those of the traditional era. This paper took diachronic and synchronic approaches in order to understand the current TKM. In the process of modernization and scientization of the TKM-which had begun in the early twentieth century-the western medical knowledge merged into the TKM. Also, as the College education of the TKM began after the Liberation from the Japanese colonial rule, the TKM scholars accepted the western medical department system to develop the TKM into professional knowledge. Meanwhile, since the late 1970s, the TKM has been influenced by the modern Traditional Chinese Medicine (TCM), and the TCM knowledge also merged into the TKM. And recently, the research methodology of the complementary and alternative medicine (CAM), and the clinical research technique of the western medicine-like Evidence based Medicine (EBM)-are affecting the scientization and clinical study of the TKM. The current TKM has been formed as a result of the hybridization of these different knowledges. These changes in the TKM were inevitable for the survival of it in the fast changing world. But these changes brought with them problems. Among those, the identity issue is the most important and crucial.

• 한국정보통신학회논문지
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• 제10권8호
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• pp.1502-1509
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• 2006

본 논문에서는 최근 홈 네트워크 기술로 관심을 받고 있는 IEEE 802.15.3 HDR-WPAN (High Data Rate-Wireless Personal Area Network) 시스템의 성능 및 용량 향상 방안으로 MIMO 기법을 적용하고 컴퓨터 시뮬레이션을 통해 성능을 분석하였다. 특히, MIMO 기법 중 독립적인 각각의 부채널들에 서로 다른 데이터를 전송하여 전송률을 높이는 V-BLAST 기법을 적용하였다. HDR-WPAN 시스템의 TCM-64QAM 모드에서 V-BLAST 기법을 적용한 경우 최소 2개의 송수신 안테나를 사용하는 것으로도 대역폭 확장 없이 110Mbps 이상의 전송속도를 얻을 수 있었으며, 레일리 페이딩 채널에서 송신 안테나 2개, 수신 안테나 2개를 사용할 경우 약 $E_b/N_0=35dB$ (MMSE 알고리즘)에서 10-4의 BER 성능을 얻을 수 있었다. V-BLAST 기법을 적용한 HDR-WPAN 시스템은 수신단에서 복조 순서(ordering)를 결정하는 과정이 다소 복잡하지만 추가적인 대역폭 확장 없이 데이터 스트림을 다수의 안테나로 전송하여 전송 용량 및 성능을 높일 수 있었다.

• 한국수정란이식학회지
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• 제26권2호
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• pp.123-128
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• 2011

In vitro maturation and activation of oocytes are primary steps towards biotechnological manipulation in embryology. The objectives of the present study were to determine the oocyte recovery rate per ovary, in vitro maturation rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats. All visible follicles were aspirated to recover follicular fluid from individual ovaries (number of ovaries = 456). The immature cumulus oocyte complexes (COCs; n = 1289) were cultured in tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal bovine serum (FBS) for 27 hours at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The matured oocytes (n = 248) were activated with 5 ${\mu}M$ ionomycin for 5 minutes followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) for 4 hours. After activation, oocytes were cultured for another 14 hours in TCM-199 supplemented with bovine serum albumin (BSA) at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The pronucleus formation in activated oocytes was determined by staining with 1% orcein (whole mount technique). Matured oocytes (n = 176) without activation stimuli were used as control. The mean number of oocytes recovered per ovary was $3.5{\pm}0.5$. The proportion of oocytes matured in vitro, confirmed by the presence of first polar body, was $42.1{\pm}4.7%$. Parthenogenetic activation, evidenced by formation of pronucleus, occurred in $37.2{\pm}15.8%$ of matured oocytes. No pronucleus formation was observed in control oocytes. In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal goats' oocytes.

• 한국동물생명공학회지
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• 제35권2호
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• pp.142-148
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• 2020

The research work was undertaken to determine an effective fertilization medium, sperm separation method and sperm capacitating agent for optimum in vitro fertilization (IVF) rates of indigenous zebu cow oocytes. In experiment 1, tissue culture medium (TCM 199), Tyrode's albumin lactate pyruvate (TALP) and Brackett and Oliphant (BO) medium were used as basic medium for IVF of oocytes of indigenous zebu cows. In experiment 2, three sperm separation methods namely centrifugation, swim up and percoll gradient methods were used for separation of motile and viable spermatozoa for IVF. In experiment 3, for capacitation of spermatozoa, IVF medium supplemented with the heparin, mixture of penicillamine, hypotaurine and epinephrine (PHE) or the combination of heparin with PHE were used for fertilization. In vitro culture (IVC) of presumptive zygotes was done in modified synthetic oviduct fluid (mSOF) medium using standard procedure 24 h after sperm-oocytes co-culture. The cleavage rate was determined to evaluate the efficacy of fertilization medium, sperm separation method and sperm capacitating agent 24 h after IVC. The cleavage rate was higher in oocytes fertilized in TALP (63.3%) than in TCM 199 (47.5%) (p < 0.05). The cleavage rate was higher in oocytes fertilized by spermatozoa separated by percoll gradient method (62.3%) than by centrifugation (51.6%) (p < 0.05). The cleavage rate of oocytes was higher when insemination was done with spermatozoa capacitated in TALP supplemented with heparin and PHE (61.3%) compared to control (40.9%) (p < 0.05). In conclusions, TALP based medium and percoll gradient sperm separation followed by capacitation with combination of heparin and PHE are suitable for IVF of indigenous zebu cow oocytes in Bangladesh.

• 한국가축번식학회지
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• 제16권3호
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• pp.239-246
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• 1992

미성숙 나포란을 20% FCS가 첨가된 TCM-199으로써 24시간 동안 5% CO2, 8% O2의 공기 조건하에서 체외성숙시킨 다음 난자의 위란강내로 정자를 미세주입하여 체외수정을 실시하였다. 미세주입 하기전 정액을 0.75% BSA가 첨가된 Ham's F-10 배양액으로 2시간 동안 5% CO2, 8% O2의 공기 조건하에서 배양함으로써 수정능 획득을 하도록하였으며 이어 30분 동안 12mM의 dbcGMP와 10mM의 imidazol이 함유된 Ham's F-10 배양액으로 배양함으로써 첨체반응을 유도하였다. 본 실험에서 정자의 미세주입 후 제 2극체와 전핵형성이 일어난 난자의 비율은 각각 9.5, 5.4%였으며 2세포기 이후로 발달한 난자의 비율은 4.1%이었다.

• 한국통신학회논문지
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• 제17권10호
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• pp.1147-1156
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• 1992

본 논문에서는 $\pi/4$ shift QPSK에 Trellis 부호화된 변조 기법(Trellis-Coded Modulation. TCM)을 적용시키기위하여 $\pi/8$ shift 8PSK를 제안하고 위상차에 의한 신호 집합 확장과 신호 집합 분할을 수행하는 trellis 부호화된 π/8 shift 8PSK를 제안한다. 또한 BER(Bit Error Rate) 성능을 향상시키기위하여 제1차 위상차뿐만아니라 제L차 이상차의 자승 유클리드 거리를 매트릭(Branch Metric)으로 갖는 비터비 디코더(Viterbi decoder)를 설계한다. 그리고 $\pi/4$ shift QPSK, trellis 부호화된 $\pi/8$ shift 8PSK와 제L차 위상차의 자승 유클리드 거리를 메트릭(Branch Metric)으로 갖는 trellis 부호화된 $\pi/8$ shift 8PSK 의 BER 특성을 AWGN에서 Monte Carlo 시뮬레이션을 통해 알아본다. 제안된 알고리즘은 MDPSK에도 적용될 수 있다.

• 한국측량학회지
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• 제37권5호
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• pp.379-388
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• 2019

Recently, a technique for acquiring spatial information data using UAV (Unmanned Aerial Vehicle) has been greatly developed. It is a very crucial issue of the GIS (Geographic Information System) mapping system that passes way point in the unmanned airframe and finally measures the accurate image and stable localization to the desired destination. Though positioning using DGPS (Differential Global Navigation System) or RTK-GPS (Real Time Kinematic-GPS) guarantee highly accurate, they are more expensive than the construction of a single positioning system using a single GPS. In the case of a low-priced single GPS system, the stability of the positioning data deteriorates. Therefore, it is necessary to supplement the uncertainty of the absolute position data of the UAV and to improve the accuracy of the current position data economically in the operating state of the UAV. The aim of this study was to present an algorithm enhancing the stability of position data in a single GPS mode of UAV with multiple GPS. First, the arrangement of multiple GPS receivers through the center of gravity of the UAV were examined. Next, MD (Mahalanobis Distance) is applied to detect instantaneous errors of GPS data in advance and eliminate outliers to increase the accuracy of previously collected multiple GPS data. Processing procedure for multiple GPS reception data by applying the center of the triangular method were presented to improve the position accuracy. Second, UAV navigation systems integrated multiple GPS through configuration of the UAV specifications were implemented. Using the unmanned airframe equipped with multiple GPS receivers, GPS data is measured with the TCM (Triangular Center Method). In addition, UAV equipped with multiple GPS were operated in study area and locational accuracy of multiple GPS of UAV with VRS (Virtual Reference Station) GNSS surveying were compared. The result showed that the error factors are compensated, and the error range are reduced, resulting in the reliability of the corrected value. In conclusion, the result in this paper is expected to realize high-precision position estimation at low cost in UAV using multiple low-cost GPS receivers.

• 한국가축번식학회지
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• 제17권2호
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• pp.149-157
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• 1993

소 초기배의 체외생산을 위한 소 난포란 회수를 극대화할 수 있는 방법을 확립하기 위해 여러 가지 방법에 의해 채취된 난자의 발생능력을 검토하였다. 전통적인 흡입법(대조구), 개발된 회수법(slicing) 및 이들을 결합한 방법(결합법)을 비교하였다. 총 245개의 난소로부터 1,641개의 난포란을 실험에 이용하였다. 회수된 난자는 TCM199과 소 태아혈청을 기초로 한 배양액에서 24시간 체외성숙시켜 급속염색법에 의해 핵성숙을 판별하고, 7% 에탄올에 의해 활성화된 처녀발생란의 전핵형성 유무에 의해 세포질 성숙을 평가하였다. 회수된 평균 난자수는 난소당 흡입법, slicing 및 결합법이 각각 1.87, 11.05 및 7.88개를 얻어 새로 개발된 slicing에 의해 회수율을 5.9배 (11.05/1.87) 증가시킬 수 있었다. 핵 성숙은 흡입법 92.9%, slicing 79.1%와 결합법 71.7%였다. 비록 흡입법에 의해 회수된 난자의 핵 성숙율이 높았지만 난소당 얻을 수 있는 성숙 난자의 수는 slicing할 경우 5배까지 증가시킬 수 있었다. 세포질 성숙의 지표인 전핵의 형성율은 대조구 75%, slicing 67%, 그리고 결합법 62.5%였다. 이같은 결과는 개발된 slicing법에 의해 도살장 난소로부터 보다 많은 수의 난자의 회수가 가능하며 이들의 핵성숙 및 세포질 성숙도 정상적으로 일어나며 난소당 전핵 초기배 수를 증가시킬 수 있음을 보여준 것이다. 아울러 증가된 난자수로 인하여 초기배의 생화학적 분석 및 외래유전자의 미세주입을 위한 지속적으로 안정된 초기배의 공급체계가 확립되었다.