• 한국응용과학기술학회지
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• 제12권1호
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• pp.69-79
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• 1995

Ham, sausage and bacon were treated with common household processing techniques including refrigerated storage(0, 14, 28 days) and cooking(pan-frying, microwaving, boiling). Lipid oxidation was evaluated by measuring fatty acid composition, malonaldehyde(MA), TBA values and by measuring fluorescent products. Major fatty acid composition were oleic acid and followed respectively palmitic acid, stearic acid, linoleic acid, linolenic acid. There was no significant difference in fatty acid composition by cooking method but there was a tendency of being increased of unsaturated fatty acid during 28days storage. Ma, TBA and fluorescent products showed a tendency of being increased continually according to storage days rather than cooking method.

• 한국식품저장유통학회지
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• 제19권5호
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• pp.639-644
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• 2012

본 실험은 형광분광법을 이용하여 분말화된 대두의 산패도를 유지의 추출과정 없이 직접 측정하는 방법을 확립하기 위해 실시하였다. FST와, TBA test, 산가 측정법을 이용하여 $25^{\circ}C$와 $90^{\circ}C$에서 20일 동안 저장하면서 산패도에 따라 값을 비교하였다. $25^{\circ}C$에서 저장 기간 중의 산패도는 변화가 없음을 알 수 있었다. 모든 측정치들은 초기값에 비해 20일 후의 값이 크게 차이 없이 일정한 것을 알 수 있었다. 반면 $90^{\circ}C$에서는 FST의 경우 8일에서 11일 사이에 FI값이 크게 증가하는 경향을 보인 후 일정하게 값이 증가하는 경향을 볼 수 있었는데 TBA test에서는 0일에서 꾸준하게 증가하던 값이 다시 감소하는 것을 보였으며 이는 oleic acid와 linoleic acid의 함량이 높은 대두의 특징이라 할 수 있다. 본 연구에서 실험한 시료의 경우 산가는 FST와 같은 경향을 보여주었다. 본 연구의 중요한 결과는 FST를 이용하여 대두의 산패를 분말상에서 직접 측정하는 법을 확립한 것이며 이는 일반적으로 사용되는 TBA test와 산가를 이용한 직접측정법에 비해 측정시간이 빠르며 추출과정에 의한 오차를 최소화 할 수 있음을 보여주었다.

• 한국식품과학회지
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• 제35권2호
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• pp.173-181
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• 2003

감마선 조사한 국산 및 중국산 옥수수 전분의 저장 중 감마선 조사여부 확인 가능성과 품질 변화를 확인하였다. 점도 측정법을 이용한 검지가능성 시험에서 시료의 점도는 조사 선량에 따라 조금씩 감소하였고, $0^{\circ}C$ 및 $20^{\circ}C$에서 9개월간 저장 중에도 비조사구와 조사구 간의 구분이 가능하였다. Specific parameter를 설정한 결과, 조사처리에 따라 그 값이 감소하여 조사 여부의 확인이 가능하였으며, 조사선량과 specific parameter 간의 상관성은 저장 중 $R^2$값이 0.89이상으로 높게 나타나 조사여부의 검지에 활용할 수 있을 것으로 생각되었다. TBA가는 감마선 조사선량에 비례하여 저장 기간에 따라 유의적으로 증가하였으며, Hunter a값은 조사선량과 유의적인 상관을 나타내었다. 이상과 같이 specific parameter를 이용한 점도측정법은 감마선 조사 후 저장 중에도 조사여부의 screening에 적용이 가능할 것으로 판단되었으며, TBA가의 측정은 판정의 정확도를 높여주는 보조수단으로 기대된다.

• Preventive Nutrition and Food Science
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• 제1권2호
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• pp.174-178
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• 1996

The content of anserine, taurine, and L-histidine was measured by HPNC in the muscle of mature(670~690g) and juvenile(80~120g) rainbow trout fatmed in Chungsun, Korea. The concentration of anserine and taurine was higher in mature rainbow trout than in juvenile, but that of L-histidine was lower in mature than in juvenile. When measured with the chemiluminescence(CL) assay, anserine and taurine showed very powerful antioxidative activity above physiological concentration rainbow trout. Taurine still showed antioxidative activity below physiological concentration, while anserine showed prooxidative activity below that. L-Histidine was prooxidative dose-dependently. In TBA method, while taurine showed very week antioxidative effect, anserine appeared very powerful antioxidant and L-histidine prooxidant at physiological concentration. There was no synergism between anserine and taurine and anserine inhibited prooxidative effect of L-histidine.

• Journal of Ginseng Research
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• 제14권3호
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• pp.373-378
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• 1990

Free malonaldehyde was determined in nine kinds of ginseng polyacetylene-treated micro- some by HPLC analysis. Antioxidant activities of some phenolic compounds and ginseng saponin were also drtermined both by a new HVLC method and by THA method. A new HPLC system separaterl malonaldehyde at a retention time 5,6 min and showed a linear relationship between the peak are a and malonaldehyde concentration. Panaxnol showed the strongest activity among nine polyacetylenes and the addition of either chlorine or aletyl group reduced polyacetylene's own activity. Since C14-polyacetylenes such as panaxyne and panaxyne-epoxide had little or no antioxidant activities, S17-structure should be preserved to exert a radical-scvenging or trapping activity. The antioxidant activities of chlorogenic acid, ferulic acid and catechol were much weaker than those of C17-polyacetylenes. Ginseng saponin showed no antioxidant activity. Since TBA reactive substances and malonaldehyde contents were almost the same in peroxiedized microsome. TBA value seems a good indicator for lipid peroxidation in this particular Fe+3 ADP/NADPH system.

• Natural Product Sciences
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• 제8권2호
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• pp.48-51
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• 2002

Antioxidative properties of fifteen anthraquinone derivatives, including eleven atural anthraquinones isolated from the roots of Morinda elliptica and four from synthetic origin were evaluated using thin layer chromatography (TLC), ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Five of the compounds, nordamnacanthal, damnacanthal, 2-formyl-1-hydroxyanthraquinone, morindone and alizarin showed higher antioxidative activity than standard natural antioxidant, ${\alpha}-tocopherol$, on the FTC assay. Morindone and alizarin showed the strongest antioxidant activity. The results from the bioassay using TBA method correlated well with the results of the FTC method.

• Bulletin of the Korean Chemical Society
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• 제23권10호
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• pp.1381-1391
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• 2002

The solvent sublation using ion pairs of metal-2-naphthoate(2-HNph) and tetrabutyl ammonium ($TBA^+$) ion has been studied for the concentration and determination of ultra trace Bi(III), In(III) and Tl(Ⅲ) ions in water samples. The partition coefficients ($K_p$) and the extraction percentages of 2-HNph and the ion pairs to methyl isobutyl ketone (MIBK) were obtained as basic data. After the ion pair $TBA^+$·M$(Nph)_4^-$ was formed in water samples, the analytes were concentrated by the solvent sublation and the elements were determined by GF-AAS. The pH of the sample solution, the amount of the ligand and counter ion added and stirring time were optimized for the efficient formation of the ion pair. The type and amount of optimum surfactant, bubbling time with nitrogen and the type of solvent were investigated for the solvent sublation as well. 10.0 mL of 0.1 M 2-HNph and 2.0 mL of 0.1 M $TBA^+$ were added to a 1.0 L sample solution at pH 5.0. After 2.0 mL of 0.2%(w/v) Triton X-100 was added, the ion pairs were extracted into 20.0 mL MIBK in a flotation cell by bubbling. The analytes were determined by a calibration curve method with measured absorbances in MIBK, and the recovery was 80-120%.

• Preventive Nutrition and Food Science
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• 제11권1호
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• pp.6-9
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• 2006

This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT(synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61 % and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2813-2818
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• 2010

A novel method was developed for the speciation of chromium in natural water samples based on homogeneous liquid-liquid extraction and determination by flame atomic absorption spectrometry (FAAS). In this method, Cr(III) reacts with a new Schiff's base ligand to form the hydrophobic complex, which is subsequently entrapped in the sediment phase, whereas Cr(VI) remained in aqueous phase. The Cr(VI) assay is based on its reduction to Cr(III) by the addition of sodium sulfite to the sample solution. Thus, separation of Cr(III) and Cr(VI) could be realized. Homogeneous liquid-liquid extraction based on the pH-independent phase-separation process was investigated using a ternary solvent system (water-tetrabutylammonium ion ($TBA^+$)-chloroform) for the preconcentration of chromium. The phase separation phenomenon occurred by an ion-pair formation of TBA and perchlorate ion. Then sedimented phase was separated using a $100\;{\mu}L$ micro-syringe and diluted to 1.0 mL with ethanol. The sample was introduced into the flame by conventional aspiration. After the optimization of complexation and extraction conditions such as pH = 9.5, [ligand] = $1.0{\times}10^{-4}\;M$, [$TBA^+$] = $2.0{\times}10^{-2}\;M$, [$CHCl_3$] = $100.0\;{\mu}L$ and [$ClO_4$] = $2.0{\times}10{-2}\;M$, a preconcentration factor (Va/Vs) of 100 was obtained for only 10 mL of the sample. The relative standard deviation was 2.8% (n = 10). The limit of detection was sufficiently low and lie at ppb level. The proposed method was applied for the extraction and determination of chromium in natural water samples with satisfactory results.

• 한국식품과학회지
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• 제28권1호
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• pp.77-82
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• 1996

소목을 여러가지 용매로 추출하여 추출물을 얻고, 75% 에탄올 추출물은 다시 용매 분획하여 Rancimat method로 항산화 효과를 비교하였으며 상업용 항산화제인 ascorbic acid, ${\delta}-tocopherol$, BHA, BHT 등과도 항산화력을 비교하였다. Ethyl acetate(EtOAc) 조추출물 600 ppm을 팜유와 돈지에 첨가시 AI (Antioxidant index; 각 항산화제를 첨가한 실험구의 유도기간을 무첨가구의 유도기간으로 나눈 값)는 각각 2.06, 7.40으로 비교적 높았으며 돈지에 대한 항산화 효과가 뚜렷하였다. 75% 에탄올 추출물을 순차 분획하여 얻은 EtOAc 분획물의 항산화 효과는 다른 분획물들 및 조추출물보다 높았고 이 분획물 600 ppm을 팜유와 돈지에 첨가했을 때 AI는 각각 2.07, 7.26으로 EtOAc 조추출물의 항산화력과 거의 같은 수준이었으나 추출 수율은 EtOAc 분획물이 2배이상 더 높았다. 소목 75% 에탄올 추출물의 EtOAc 분획물과 상업용 항산화제 일정량을 팜유와 돈지에 첨가한후 $60^{\circ}C$로 보관하면서 POV와 TBA를 측정하여 항산화 효과를 비교한 결과, 분획물 200 ppm 첨가시의 항산화력은 BHA 및 TO 200 ppm보다 높았고 BHT 200 ppm과 거의 같은 수준이었다. EtOAc 분획물 500 ppm의 항산화력은 BHT 200 ppm보다 높았다.