• 한국식품위생안전성학회지
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• 제5권4호
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• pp.237-242
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• 1990

Streptozotocin에 의한 적혈구의 막 lipid peroxidation 측정시 TBA법의 적합성에 관하여 검토하기 위하여 Streptozotocin와 TBA의 직접반응 여부를 연구하였다. 여러 농도의 Streptozotocin 와 20% RBC suspension을 함께 배양한 후 lipid peroxidation을 TBA법으로 측정했을 때, Streptozotocin의 농도에 따라 흡광도가 증가하다가 100mM부터는 plateau를 이루었다. 그런데 적혈구를 제거한 대조실험에서도 발색단이 관찰되었다. 시험관에서 5mM의 Streptozotocin와 1% TBA를 산성조건에서 가열 직접 반응시킨 후 spectrum을 찍은 결과 TBA법 측정파장인 532nm에서 새로운 peak가 생겼다. 또 이 파장에서의 흡광도는 Streptozotocin 농도에 따라 증가하다가 25mM부터는 plateau가 되었다. 이러한 결과들은 적혈구와 Streptozotocin를 함께 배양하여 lipid peroxidation을 측정할 때, cell 내로 유입된 Streptozotocin 가 TBA와 직접반응을 할 수 있음을 제시한다. 그리고 다른 측정법인 lipid conjugation은 233nm에서의 흡수를 측정하는데. Streptozotocin 자체가 200-300nm에서 흡수를 나타내므로 이 방법으로도 lipid peroxidation 측정을 방해할 수 있다. 따라서 Streptozotocin에 의한 적혈구막의 lipid peroxidation은 TBA method 및 lipid conjugation 방법으로 측정하기는 부적합한 것으로 사료된다.

• Preventive Nutrition and Food Science
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• 제4권3호
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• pp.171-174
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• 1999

For monitoring lipid oxidation development in cooked ground pork during refrigerationm, malonaldehydethiobarbituric acid(MA-TBA) contents were measured using high performance liquid chromatography(HPLC). As the oxidation proceeded during refergeration, TBA-reaction substances(TBARS) absorbances increased and the corresponding HPLC peak areas also increased proportationately. The correlation coefficient between the HPLC peak areas and MA-TBA absorbance were 0.9979. The treatemtn of cetrimide, an ion pairing agent, gave a complete resolution of the MA-TBA complex and the butanol extraction of the complex increased its recovery by 37.8%. Both cetrimide treatment and butanol extraction are essential steps for analyzing MA-TBA complex in ground pork wiht HPLC. A reliable and specific measurement of NA-TBA in ground pork was successfully performed using HPLC.

• Journal of Nutrition and Health
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• 제17권1호
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• pp.31-40
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• 1984

혐기적 조건밑에서 $FeCl_{3}$에 의해 촉진된 TBA 반응을 이용하여 혈청중 과산화물 측정을 위한 새로운미량 분석법을 설정하였다. 즉. 혈청단백침전물이 현탁된 TBA반응흔합액에 혈청 $10{\mu}l$당 $1.0{\mu}mole$의 $FeCl_{3}$를 첨가한 다음 질소기류밑에서 50분간 끊는 물에 중탕한다. 이 분석법의 반응 예민도는 현행 일반 TBA법보다 40여배나 증대되었으며 따라서 미량의 시료$(10{\sim}20{\mu}l)$로도 TBA반응생성물의 농도 측정이 비색법으로도 가능하였다. 그리고 TBA반응생성물의 butanol 추출액을 $(NH_{4})_{2}SO_{4}$염석으로 부분말수시킴으로써 예민성 및 재현성이 개선될 수 있었다. 이 분석법을 이용하여 측정한 건강인들의 혈청 TBA같은 높은 연령군일수록 증가하는 경향을 보였다.

• 한국축산식품학회지
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• 제20권4호
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• pp.288-295
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• 2000

동결기간과 해동후 재냉장이 우육의 지방산 조성. TBA가 생육향 및 가열육향에 미치는 영향을 파악하기 위하여 홀스타인의 등심부위를 -2$0^{\circ}C$에서 6, 12 및 18개월 동경, 해동한 것과 이것을 1$^{\circ}C$에서 3일간 재냉장한 것에 대하여 실험하였다. 신선육의 총지질 중성지질 및 인지질의 단일 불포화지방산에 대한 포화지방산의 비율(MUFA/SFA)은 각각 1.35, 1.10 및 1.34이었으며 동결기간이 길어지면서 점점 감소하였다. 동결 6개월째 우육은 TBA가는 0.37 mg/MA/kg으로 신선육의 0.15 mg/MA.kg 보다 높아졌으나 산패취는 느끼지 못했다. 동결 12개월과 18개월째 우육의 TBA가는 각각 0.53과 0.64mg/MA/kg이었으며 산패취를 느낄수 있었다. 한편 재냉장한 우육은 동결기간에 관계없이 TBA가가 높아지고 생육향 평가 점수가 낮아졌으나 MUFA/SFA와 가열육향은 동결 6개월째에 향상되었고 12개월 및 18개월 째에는 저하되어 동결기간에 따라 재냉장 효과의 차이를 보였다.

• Preventive Nutrition and Food Science
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• 제4권3호
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• pp.167-170
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• 1999

Various concentrations of malonaldehyde (MA) produced upon hydrolysis of 1, 1, 3,3-tetraethoxypropane (TEP) were reacted with 2-thiobarbituric acid (TBA)and th e contents of MA-TBA complex were measured both with spectrophotometer and high performance liquid chromatography (HPLC). As the concentrations of MA-TBA increased, their absorbances and the corresponding HPLC peak areas increased. The correlation coefficient between absorbances and HPLC peak areas of MA-TBA peaks from the other compounds and butanol extraction of the complex increased its recovery its recovery by 29.4% . Measurement of the content of MA-TBA complex for monitoring the development of lipid oxidation was proven to be successful with the use of high performance liquid chromatography.

• 한국염색가공학회지
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• 제15권3호
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• pp.127-131
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• 2003

Acrylonitrile(AN) was solution-polymerized in dimethyl sulfoxide(DMSO) and tertiary butyl alcohol(TBA) at 30, 40, $50^\circ{C}$ using a low temperature initiator, 2,2'-azobis(2,4-dimethylvaleronitrile) (ADMVN). The low temperature polymerization using ADMVN, DMSO, and TBA is to be successful in obtaining high molecular weight polyacrylonitrile(PAN) with less branches by solution polymerization. Throug a polymerization of AN in DMSO at $30^\circ{C}$, PAN having viscosity-average molecular weight$(M_v)$ of 931,000 was obtained. And then, during AN solution polymerization in DMSO and TBA using a cosolvent system the in-situ formation of microfibrillar structure has been discovered at the cosolvent composition of 24/1$(V_{DMSO}/V_{TBA})$. The simultaneous process of gelation and phase separation of long chain molecules may explain the in-situ formation of PAN fibers during polymerization.

• 폴리머
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• 제24권5호
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• pp.610-620
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• 2000

아세트산비닐(VAc)을 아조비스디메틸발레로니트릴(ADMVN) 및 삼차부틸알코올 (TBA)을 각각 개시제 및 용매로 하여 30, 40 및 5$0^{\circ}C$에서 용액중합하였다. 합성된 폴리아세트산비닐 (PVAc)을 비누화함으로써 고분자량 혼성배열 폴리비닐알코올(PVA)을 제조하였다. 중합조건들이 전환률, 가지화도 및 PVAc와 PVA의 분자량에 미치는 영향을 고찰하였다. TBA에서의 VAc의 중합 속도는 ADMVN 농도의 0.49승에 비례하였고, 이는 이론치 0.5와 잘 일치하였다. 저온에서 개시가 가능한 ADMVN 및 낮은 사슬이동상수를 갖는 TBA를 사용함으로써 고분자량 및 고수율의 PVA가 얻어졌다. PVAc의 평균 중합도는 전환률 약 35%부터 70%의 범위에서 10000~13000이었고, 이를 비누화하여 얻은 PVA의 평균 중합도는 2400~6100이었다. 교대배열 다이애드기 함량은 중합온도를 낮춤에 따라 조금씩 증가하였고, 중합시 TBA의 입체장애 효과 때문에 TBA의 양을 증가시킴에 따라서도 증가하였다.

• 한국환경보건학회지
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• 제34권5호
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• pp.387-394
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• 2008

The toxicity of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on the indigenous microbial community in forest soil was studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P<0.05). FA toxicity was the highest, but TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated MTBE, TBA and FA were grouped by exposure time not exposure concentration. Dominant species in the microcosms were as follows: Photobacterium damselae sub sp. and Bacillus sp. KAR28 for MTBE; Mycobacterium sp. and Uncultured Clostridium sp. for TBA; and Uncultured Paenibacillaceae bacterium and Anxynobacillus, Flavithermus for FA.

• Applied Microscopy
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• 제28권3호
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• pp.299-306
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• 1998

T-butyl alcohol (TBA) freeze-drying method originally designed by Inoue and Osadake (1989) was adopted to dry specimens for scanning electron microscopy and the results were compared with those dried using critical point dryer (CPD). Small pieces $(1\times1\times3mm)$ of liver of Sprague-Dawley rats were cut and fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer after anesthesia, and processed for scanning electron microscopy by several modifications of TBA freeze-drying methods and by the standard preparation method using CPD. The bile canaliculi and sinusoidal endothelial surface were observed. Tissue dehydrated with TBA before TBA freeze-drying preserved the structures best comparable to those prepared with CPD. This result suggests that combination of dehydration with TBA and TBA freeze-drying is a superior method to the original TBA freeze-drying method dehydrated with ethanol.

• Current Photovoltaic Research
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• 제2권3호
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• pp.124-129
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• 2014

Dye-sensitized solar cells (DSSCs) are considered as promising alternatives to conventional photovoltaic device. However, commercialization of the DSSCs is restricted due to the low efficiency. In this paper, highly efficiency DSSCs were fabricated by the adding the TBA to the $TiO_2$ paste. $TiO_2$ photoanode added 0.2 M TBA in DSSCs are shown the best efficiency of 9.14 %. This result ascribed to improvement of the connection between the $TiO_2$ nanoparticles by the addition of the optimized amount TBA. The morphology of the photoanode was observed by FE-SEM. Further investigation about the kinetics of the electrochemical processes are performed by the EIS analysis. Longest diffusion length was obtained in case adding 0.2 M of TBA to $TiO_2$ paste, which was matched well with the improved efficiency.