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Evaluation of the Fiber Separation Method and Differences in the Storage Root Fiber Content among Sweetpotato (Ipomoea batatas L.) Varieties (고구마 괴근의 섬유질 분리 조건 탐색 및 품종별 섬유질 함량 차이)

  • Won Park;Im been Lee;Mi Nam Chung;Hyeong-Un Lee;Tae Hwa Kim;Kyo Hwui Lee;Sang Sik Nam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.1
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    • pp.20-26
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    • 2023
  • Fiber content in the storage roots of sweetpotato varies between different varieties. For examples, the high fiber content of certain types has a poor texture when steamed or roasted. This study was conducted to evaluate the optimal sieve mesh size for separating fibers, the chemical composition of fibers and differences in fiber content among different varieties. We found that the separated fiber content (dry weight) of mashed and steamed sweetpotato was higher after washing three times (143.3 mg/100 g) compared with that washed five times (128.4 mg/100 g). The Hogammi variety remained 85.9% of total fiber content at 10 mesh (2,000 ㎛) and 9.6% of total fiber content at 30 mesh (600 ㎛), and Jinyulmi remained 74.9 and 16.7% of total fiber content , respectively. Therefore, a 30 mesh sieve was considered the most suitable for fiber separation. Among the 10 studied cultivars, Jinhongmi showed the lowest amount of fiber (24.8 mg/100 g) and Hogammi had the highest amount (111.4 mg/100 g), which was 4.5 times larger than that of Jinhongmi. Cellulose, hemicellulose and lignin content of separated fibers showed no difference between the viscous-type Hogammi and powdery-type Jinyulmi varieties, with averages of 32.5, 22.3 and 29.6%, respectively. Correlation results using the Image J program showed a significant correlation between the distribution of the stained area and the fiber content (R = 0.74, p < 0.05). Staining distribution differed among varieties, suggesting that a simple fiber content test could be performed using the staining method on raw sweetpotato. These results provide useful information to help inform farmers on the fiber content of different sweetpotato varieties.

Response of Yield and Quality in Major Domestic Rice (Oryza sativa L.) Varieties according to the Nitrogen Application Levels (질소시비수준에 따른 국내 주요 벼 품종의 수량 및 품질 반응)

  • Jong-Seo Choi;Jinseok Lee;Shingu Kang;Dae-Woo Lee;Woonho Yang;Seuk-Ki Lee;Su-Hyeon Sin;Min-Tae Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.67 no.4
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    • pp.342-361
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    • 2022
  • In order to evaluate the effect of nitrogen application levels on yield and quality of rice varieties, a field experiment was conducted at National Institute of Crop Science of Korea from 2018 to 2020. Five levels (0, 3, 5, 7, and 9 kg/10a) of nitrogen fertilizer were treated to 21 Korean rice varieties. Yield, yield component, appearance quality, and protein content in rice were analyzed. The average head rice yield for 3 years decreased by 28%, 22%, 11%, and 8%, respectively, when cultivated with 0, 3, 5, and 7 kg/10a nitrogen application compared to cultivation with a standard nitrogen application amount, 9 kg/10a. The number of panicles per hill increased as the amount of nitrogen application increased, but there was no significant change in the number of grains per panicle and 1000-grains weight, and the number of panicles per hill showed relatively small annual variation compared to other yield components. There was no significant difference in the head rice ratio according to the nitrogen application amount, the broken rice ratio slightly decreased, and the floury rice ratio increased. The protein content of rice decreased with increasing nitrogen application in 2018 and 2019, and was the lowest at 7 kg/10a of nitrogen application, and showed a tendency to increase again at 9 kg/10a. In the case of 2020, as the amount of nitrogen application increased, the protein content showed a tendency to continuously increase. In terms of varieties, 13 varieties, including Chilbo, seemed to be capable of low-nitrogen cultivation because loss of the head rice yield was less and the protein content could be lowered to 6% or less according to 7 kg/10a nitrogen application.

Polymerization of dual cured composites by different thickness (두께에 따른 이중 중합형 복합레진의 중합)

  • Kim, Yun-Ju;Jin, Myoung-Uk;Kim, Sung-Kyo;Kwon, Tae-Yub;Kim, Young-Kyung
    • Proceedings of the KACD Conference
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    • 2008.05a
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    • pp.169-176
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    • 2008
  • The purpose of this study was to evaluate the effect of thickness, filling methods and curing methods on the polymerization of dual cured core materials by means of microhardness test. Two dual cured core materials, MultiCore Flow (Ivoclar Vivadent AG, Schaan, Liechtenstein) and Bis-Core (Bisco Inc., Schaumburg, IL, USA) were used in this study. 2 mm (bulky filled), 4 mm (bulky filled), 6 mm (bulky and incrementally filled) and 8 mm (bulky and incrementally filled)-thickness specimens were prepared with light cure or self cure mode. After storage at $37^{\circ}C$ for 24 hours, the Knoop hardness values (KHN) of top and bottom surfaces were measured and the microhardness ratio of top and bottom surfaces was calculated. The data were analyzed using one-way ANOVA and Scheffe multiple comparison test, with ${\alpha}=0.05$. The effect of thickness on the polymerization of dual cured composites showed material specific results. In 2, 4 and 6 mm groups, the KHN of two materials were not affected by thickness. However, in 8 mm group of MultiCore Flow, the KHN of the bottom surface was lower than those of other groups (p < 0.05). The effect of filling methods on the polymerization of dual cured composites was different by their thickness or materials. In 6 mm thickness, there was no significant difference between bulk and incremental filling groups. In 8 mm thickness, Bis-Core showed no significant difference between groups. However, in MultiCore Flow, the microhardness ratio of bulk filling group was lower than that of incremental filling group (p < 0.05). The effect of curing methods on the polymerization of dual cured composites showed material specific results. In Bis-Core, the KHN of dual cured group were higher than those of self cured group at both surfaces (p < 0.05). However, in MultiCore Flow, the results were not similar at both surfaces. At the top surface, dual cured group showed higher KHN than that of self cured group (p < 0.05). However, in the bottom surface, dual cured group showed lower value than that of self cured group (p < 0.05).

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Integrated Mechanization System on Polyethylene Film Mulching Culture in Sesame (참깨의 비닐피복 기계화 일관작업 체계 연구)

  • Kang, Chul-Whan;Lee, Byoung-Kyu;Ahn, Byoung-Ok;Park, Choong-Bum;Roh, Jae-Hwan;Lee, Seong-Woo;Lee, Seung-Tack;Hong, Jong-Tae;Lee, Sun-Ho;Kim, Seok-Hyeon;Lee, Sang-Chuel;Kim, Wan-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.5
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    • pp.489-496
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    • 1997
  • This study was carried out to develop and investigate integrated mechanization system on polyethylene(P.E.) film mulching culture in sesame. Field trials were carried out to develop optimum mechanics for each step of manual operation in sesame culture and demonstrated those developed mechanics of sesame dibbling vinyl mulcher, sesame binder, sesame thresher and sesame grader at the farmer, s field of Hwasong (Kyunggi-do) in 1994 and 1995. Newly developed sesame dibbling vinyl mulcher brought saving manpower 280 hrs /ha for sowing and P.E. film mulching with it's 98% of labour saving for the harvesting operation of sesame. Sesame Binder showed 93% labour saving for cutting and binding with only 22 hrs /ha compared to 330 hrs /ha that of conventional. Sesame thresher was appeared to have 85% effects of labour saving for threshing with completely dry sesame bundles at one time by 23 hrs /ha compared to 151 hrs /ha that of conventional. Sesame grader was appeared to have 72% effects for sesame grain grading with 12 hrs /ha compared to that of conventional 54 hrs /ha. Grain yield of integrated mechanization culture system showed 6% higher than that of coventional. Integrated mechanization culture system (sesame dibbling vinyl mulcher + sesame binder + sesame thresher + sasame grader) showed 62% of labour saving effects through whole steps of sesame culture by 472 hrs /ha compared to that of conventional 1, 230 hrs /ha.

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Comparison of Growth Characteristics and Nutritive Value of Silage Corn Hybrids Grown at Paddy and Upland Field (논과 밭에서 재배한 사일리지용 옥수수의 생육특성 및 영양가치 비교)

  • Young Sam Go;Jun Young Ha;Hwan Hee Bae;Jae Han Son;Kyeong Min Kang;BeomYoung Son;Tae Wook Jung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.4
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    • pp.249-257
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    • 2022
  • This study was conducted to comparison analysis growth characteristics, yield and forage quality of 18silage corn varieties grown in paddy and upland fields. In this condition, days to silking of Sinhwangok was the shortest with 78 days and Kangdaok the longest with 92 days. Moreover it was confirmed that the difference of days to silking at this condition greatly occurred in the late species than in the precocious species. The height and ear height ratio of corn grown in paddy decreased by 5~10% and increase by 10~15% compared to upland, respectively. It was confirmed that the ear ratio of corn which enhances the forage quality was highest in Sinhwangok at paddy and upland and most of the varieties decreased by 10~30% at paddy rather than upland. The length of ear was also reduced by 10~25%. In fresh yield, Dacheongok showed the highest yield at 65,750 kg/ha in upland and 38,830 kg/ha in paddy. Similar to the fresh yield, dry and TDN yield in Dacheongok also showed the highest yield at 36,910 kg/ha, 18,040 kg/ha in upland and 21,670 kg/ha, 14,390 kg/ha in paddy, respectively. As a result of analyzing crude protein and starch from seeds to evaluate the feed value of corn for silage, there was no difference between cultivars grown in this condition. In addition, as a result of analyzing the contents of ADF and NDF using leaves, stems and seeds, the contents of leaves and stems were lower in P3394 and P1543 at the upland and lower in Singwangok and Daanok when cultivated at paddy. Moreover ADF and NDF contents were decreased in some varieties in paddy rather than upland. Therefore, there is no significant difference in the feed value of corn varieties grown in this condition, it is possible to cultivate corn in paddy if normal growth is achieved through drainage management.

Evaluation Antioxidant and Anti-inflammatory Activity of Ethanolic Extracts of Myriophyllum spicatum L. in Lipopolysaccharide-stimulated RAW 264.7 Cells (이삭물수세미(Myriophyllum spicatum L.) 에탄올 추출물의 항산화와 항염증 효과)

  • Chul Hwan Kim;Young-Kyung Lee;Min Jin Kim;Ji Su Choi;Buyng Su Hwang;Pyo Yun Cho;Young Jun Kim;Yong Tae Jeong
    • Korean Journal of Plant Resources
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    • v.36 no.1
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    • pp.15-25
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    • 2023
  • Myriophyllum spicatum L. has been used as an ornamental in ponds and aquariums, and as a folk remedy for inflammation and pus. Nevertheless, the biological activity and underlying mechanisms of anti-inflammatory effects are unclear. This study is aimed at investigating the antioxidative and anti-inflammatory activities of ethanol extract of Myriophyllum spicatum L. (EMS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Antioxidant activity of EMS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. As inflammatory response parameters produced by LPS-stimulated RAW 264.7 cells were quantified to assess the anti-inflammatory activity of EMS. Our results showed that EMS increased FRAP and DPPH radical-scavenging activity. In EMS-treated RAW 264.7 cells, the production of NO, PGE2, TNF-α and IL-1β was significantly inhibited at the non-cytotoxic concentration. In addition, EMS significantly attenuated LPS-stimulated the toll-like receptor (TLR) 4/myeloid differentiation protein (MyD) 88 signaling pathway, and inhibited nuclear translocation of nuclear factor-kappa B(NF-κB). Positive correlations were noted between anti-inflammatory activity and antioxidant activity. In conclusion, it was indicated that EMS suppresses the transcription of inflammatory factors by inhibiting the TLR4/MyD88/NF-κB signaling pathway, thereby suppressing LPS-stimulated inflammation in RAW 264.7 cells. This study highlights the potential role of EMS against inflammation and associated diseases.

Effects of Acylase Treatment Episodes on Multispecies Biofilm Development (Acylase의 처리 시기 및 기간이 다종 생물막 형성에 미치는 영향)

  • Ji Won, Lee;So-Yeon, Jeong;Tae Gwan, Kim
    • Microbiology and Biotechnology Letters
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    • v.50 no.4
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    • pp.548-556
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    • 2022
  • Acylases can have a significant effect on biofilm formation owing to their quorum quenching activity. In this study, we investigated the effects of acylase treatment episodes on multispecies biofilm development. A consortium composed of 9 species belonging to different genera was allowed to form biofilms for 5 days under various treatment episodes (different treatment periods, 1, 2, 3, or 4 days; and two application timings, beginning or later) at 1, 5, 10, 20 and 50 mg·l-1 acylase concentrations. The acylase treatment for 5 days showed that acylase concentration was negative with biofilm development (linear regression, Y = -0.05·x + 2.37, p < 0.05, R2 = 0.88). Acylase was more effective in reducing biofilm formation when it was applied in the beginning (vs. in later development stage) at all acylase concentrations (p < 0.05). ANOVA indicated that treatment period was significant on biofilm formation in both application timings at ≥ 10 mg·l-1 (p < 0.05). Linearity test results showed that all slope values between period and biofilm were negative in both timings at ≥ 10 mg·l-1 (p < 0.05, except for the later application at 20 mg·l-1). When temporal biofilm dynamics were monitored at 20 mg·l-1, biofilms gradually increased with time at all treatment episodes (p < 0.05), and slope values in linear regression between biofilm and time were lower when acylase was applied in the beginning (p < 0.05). Our findings suggest the importance of the acylase treatment period and application timing on biofilm control.

Comprehensive and synthetic inventory of Dokdo Island, Republic of Korea

  • Ui Wook Hwang;Hyun Soo Rho;Bia Park;Eun Hwa Choi;Cho Rong Shin;Sa Heung Kim;Jongrak Lee;Hack Cheul Kim;Mann Kyoon Shin;Taeseo Park;Jumin Jun;Heegab Lee;Jong Eun Lee;Yoon Sik Oh;Jung-Goo Myoung;Chang Geun Choi;Jin Hee Park;Seon-joo Park;Jimin Lee;Jaeho Lee;Hyeok Yeong Kwon;Kyu Tae Park;Chun Woo Lim;Seung Wook Jung;Mi Jin Lee;Yucheol Lee;Yeongheon Shin;Hee-Jung Choi;Young Wook Lee;Hyun Jong Kil;Jin-Han Kim;Myung-Suk Kang;Eun-Young Lee;Sang-Hwa Lee;Young Hyo Kim;Jongwoo Jung;Kuem Hee Jang;Young Jin Lim;Shi Hyun Ryu;Won-Gi Min;Joo Myun Park;Hyojin Lee;Minsu Woo;Yun-Bae Kim;Sehun Myoung
    • Journal of Species Research
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    • v.12 no.spc
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    • pp.1-69
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    • 2023
  • This study aims to establish a comprehensive, synthetic inventory system for the fauna and flora of Dokdo Island, Republic of Korea, which has been conducted by a specialized research group consisting of more than 50 experts. The research was conducted over five years(2015-2019) and supported by the National Institute of Biological Resources, Ministry of Environment, Republic of Korea. All possible publications on the fauna and flora of Dokdo Island over the last 68 years from 1952 to 2020 were reviewed. As a result, 1,302 species were found on Dokdo Island during the study period. An updated list of 1,963 species was created. This is expected to be of great help for the conservation and national publicity of important indigenous biological resources of Dokdo Island.

Monitoring of Residual Pesticides and Exposure Assessment of Olive Oil Products Sold on the Market (올리브유의 잔류농약 모니터링 및 노출량 조사)

  • Mi-Hui Son;Jae-Kwan Kim;You-Jin Lee;Ji-Eun Kim;Eun-Jin Baek;Byeong-Tae Kim;Seong-Nam Lee;Myoung-Ki Park;Yong-Bae Park
    • Journal of Food Hygiene and Safety
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    • v.38 no.4
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    • pp.211-216
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    • 2023
  • A total of 100 commercially available olive oil products were analyzed for 179 pesticide residues using gas chromatography-tandem mass spectrometry (GC/MS/MS). The olive oil samples were mixed with organic solvents, centrifuged and frozen to remove fat, and pesticide residues were analyzed using the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) method. The determination coefficient (R2) of the analysis method used in this study was ≥0.998. The detection limit of the method ranged 0.004-0.006 mg/kg and its quantitative limit ranged 0.012-0.017 mg/kg. The recovery rate (n=5) measured at the level ranging 0.01-0.02, 0.1, and 0.5 mg/kg ranged 66.8-119.5%. The relative standard deviation (RSD) was determined to be ≤5.7%, confirming that this method was suitable for the "Guidelines for Standard Procedures for Preparing Food Test Methods". The results showed that a total of 151 pesticides (including difenoconazole, deltamethrin, oxyfluorfen, kresoxim-methyl, phosmet, pyrimethanil, tebuconazole, and trifloxystrobin) were detected in 64 of the 100 olive oil products. The detection range of these pesticide residues was 0.01-0.30 mg/kg. The percentage acceptable daily intake (%ADI) of the pesticides calculated using ADI and estimated daily intake (EDI) was 0.0001-0.1346, indicating that the detected pesticides were present at safe levels. This study provides basic data for securing the safety of olive oil products by monitoring pesticide residues in commercially available oilve oil products. Collectively, the analysis method used in this study can be used as a method to analyze residual pesticides in edible oils.

Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea (메타전사체 분석을 이용한 국내 대추나무의 바이러스 감염실태)

  • Hong-Kyu Lee;Seongju Han;Sangmin Bak;Minseok Kim;Jean Geung Min;Hak ju Kim;Dong Hyun Kang;Minhui Kim;Wonyoung Jeong;Seungbin Baek;Minjoo Yang;Taegun Lim;Chanhoon An;Tae-Dong Kim;Chung Youl Park;Jae Sun Moon;Su-Heon Lee
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.276-285
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    • 2023
  • This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.