• 한국환경성돌연변이발암원학회지
• /
• 제18권1호
• /
• pp.26-31
• /
• 1998

Antimutagenic activity of Saururus chinesis (Lour.) Bail was investigated for food-borne mutagens using S. typhimurium TA98. Methanol extract from Saururus Chinesis (Lour.) Bail was fractionated into hexane, chloroform, ethylacetate and butanol fractions, followed by determination of antimutagenic activity for food-borne mutagenic heterogenic amines (HCA). The hexane fraction exhibited a strong antimutagenic activity for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline (MeIQ), 2-amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3-amino-1-methyl-5H-pyroid[4,3-b]indole acetate (Trp-2-A); however its fraction rather enhanced the bacterial mutagenicity of 2-amino-3,4,8-trimethyl-3H-imidazo[4,5-f]quinozaline (4,8-diMeIQx) and 2-amino-3,7,8-trimethyl-3H-imidazo[4,5-f]quinoxline (7,8-diMeIQx). Active principle in the fraction was found to be two major compounds (${\gamma}$-crene B and epi-bicyclosesquiphellandrane) and 6 minor compounds (${\delta}$-caryophyllene, ${\gamma}$-elemene, ${\beta}$-cabebene, ${\delta}$-cadinene, ${\delta}$-selinene, and patchoulene). Modulation effect for the mutagenic activity of the food-borne mutagenic HCA by the fraction might be derived from a cumulative effect of each individual compounds. Hence, this hexane fraction might be use to reduce the production of mutagenic HCA during cooking process of protein-rich foods.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6575-6580
• /
• 2014

This study aimed to evaluate the antimutagenic and anticarcinogenic activity of turmeric essential oil as well as to establish biochemical mechanisms of action. Antimutagenicity testing was accomplished using strains and known mutagens with and without microsomal activation. Anticarcinogenic activity was assessed by topical application of 7, 12 - dimethylbenz[a]anthracene (DMBA) as initiator and 1% croton oil as promoter for the induction of skin papillomas in mice. Inhibition of p450 enzymes by TEO was studied using various resorufins and aminopyrene as substrate. Turmeric essential oil (TEO) showed significant antimutagenic activity (p<0.001) against direct acting mutagens such as sodium azide ($NaN_3$), 4-nitro-O-phenylenediamine (NPD) and N-methyl-N-nitro N'nitrosoguanine (MNNG). TEO was found to have significant antimutagenic effect (>90%) against mutagen needing metabolic activation such as 2-acetamidoflourene (2-AAF). The study also revealed that TEO significantly inhibited (p<0.001) the mutagenicity induced by tobacco extract to Salmonella TA 102 strain. DMBA and croton oil induced papilloma development in mice was found to be delayed and prevented significantly by TEO application. Moreover TEO significantly (P<0.001) inhibited isoforms of cytochrome p450 (CYP1A1, CYP1A2, CYP2B1/2, CYP2A, CYP2B and CYP3A) enzymes in vitro, which are involved in the activation of carcinogens. Results indicated that TEO is antimutagenic and anticarcinogenic and inhibition of enzymes (p450) involved in the activation of carcinogen is one of its mechanisms of action.

• 한국수산과학회지
• /
• 제27권2호
• /
• pp.133-139
• /
• 1994

식품의 가공 저장 및 조리 중에 일어나는 식품성분간의 반응 중 대표적인 반응으로 알려져 있는 Maillard 반응생성물의 돌연변이원성에 대한 그 억제 기구를 밝히기 위하여 glucose-arginine계 및 glucose-lysine${\cdot}$HCl계 Maillard 반응 생성물에 일상 생활에서 많이 섭취하고 있는 해조류 및 야채류의 수용성 추출물을 첨가하여 그 억제효과를 조사하였고, 아울러 이들 수용성추출물의 가열($100^{\circ}C$, 10분)에 따른 억제효과의 영향에 대해 조사 검토하였다. 그 결과, 실험에 사용한 해조류 및 야채류 수용성 추출물 전부가 돌연변이원성 억제효과를 나타내었고, 특히 양배추, 파, 산초, 및 청각에 있어서 억제효과가 높게 나타났다. 가열한 경우, 야채류의 돌연변이원성 억제효과는 감소하였으나 해조류는 크게 영향을 받지 않았다. 이 결과로 미루어 보아 당-아미노산계 Maillard 반응생성물의 돌연변이원성 억제에 해조류 및 야채류 추출물에 존재하는 환원성 물질, 고분자 물질 catalase 및 peroxidase와 같은 효소 등이 일조를 하는 것으로 나타났다.

• Journal of Nutrition and Health
• /
• 제39권8호
• /
• pp.801-807
• /
• 2006

To elucidate the effect of soy isoflavone supplementation on bone mineral density and antioxidant enzyme activity in 60 postmenopausal women residing in Iksan area were recruited. There were 31 participants in the treatment group and 29 in the control group. The treatment group consumed isoflavone extract capsules daily (which contained 90 mg of soy isoflavones) for 12 weeks. The study compared before and after isoflavone intake in the following areas. Physical examination, diet survey, bone mineral density (BMD) and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TA)). The average age of the treatment group was 64.6 years and that of the control group was 66.5 years. There were no significant differences between the two groups in terms of height, weight and body mass index. Both groups maintained a regular diet pattern in terms of their average daily nutrient intake. There were no significant differences between the treatment group (23.3 mg) and the control group (24.0 mg) in terms of daily isoflavone intake based on diet. Isoflavone supplementation of 12 weeks did not resulted in any significant changes in BMD or parameters of antioxidant enzyme activity, implying the necessity of more intensive intervention for a substantial change. In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the bone mineral density in postmenopausal women and further systematic research on dose and period of isoflavone supplementation is needed to clearify the positive effect of isoflavone on BMD and/or blood antioxidant capacity in postmenopausal women.

• 한국식품영양과학회지
• /
• 제21권3호
• /
• pp.308-313
• /
• 1992

쑥의 주요 휘발성분이 Salmonella typhimurium TA100에서 aflatoxin B$_1$의 돌연변이 유발성에 미치는 영향을 조사하기 위해 쑥(Artemisia asictica nakai)의 생엽과 볶음 쑥차로 부터 휘발성향기성분을 Tenax trap으로 포집하여 분리하였다. GC-MS로 동정된 9가지 주요화합물중 myrcene, cineole camphor, caryophyllen, coumarin, farnesol은 돌연변이 유발을 억제시키는 효과가 있었으며, 2-pyrrolidine, thujone, 그리고 1-acetylpiperidine은 증가시키는 효과가 있었다. 그러나 이들 9가지 화합물을 혼합하여 시험하였을 때는 돌연변이 억제효과만 현저하였다. 따라서 2-pyrrolidine, thujone, 그리고 1-acetylpiperidine의 돌연변이 증가효과는 쑥중의 항돌연변이 물질에 의해 그 효과를 나타내지 못함을 알 수 있었다.

• 한국결정성장학회:학술대회논문집
• /
• 한국결정성장학회 1996년도 The 9th KACG Technical Annual Meeting and the 3rd Korea-Japan EMGS (Electronic Materials Growth Symposium)
• /
• pp.5-18
• /
• 1996

The solid state cesium ion source os alumino-silicate based zeolite which contains cerium. The material is an ionic conductor. Cesiums are stably stored in the material and one can extract the cesiums by applying electric field across the electrolyte. Cesium ion bombardment has the unique property of producing high negative ion yield. This ion source is used as the primary source for the production of a negative ion without any gas discharge or the need for a carrier gas. The deposition of materials as an ionic species in the energy range of 1.0 to 300eV is recently recognized as a very promising new thin film technique. This energetic non-thermal equilibrium deposition process produces films by “Kinetic Bonding / Energetic Condensation" mechansim not governed by the common place thermo-mechanical reaction. Under these highly non-equilibrium conditions meta-stable materials are realized and the negative ion is considered to be an optimum paeticle or tool for the purpose. This process differs fundamentally from the conventional ion beam assisted deposition (IBAD) technique such that the ion beam energy transfer to the deposition process is directly coupled the process. Since cesium ion beam sputter deposition process is forming materials with high kinetic energy of metal ion beams, the process provider following unique advantages:(1) to synthesize non thermal-equilibrium materials, (2) to form materials at lower processing temperature than used for conventional chemical of physical vapor deposition, (3) to deposit very uniform, dense, and good adhesive films (4) to make higher doposition rate, (5) to control the ion flux and ion energy independently. Solid state cesium ion beam sputter deposition system has been developed. This source is capable of producing variety of metal ion beams such as C, Si, W, Ta, Mo, Al, Au, Ag, Cr etc. Using this deposition system, several researches have been performed. (1) To produce superior quality amorphous diamond films (2) to produce carbon nitirde hard coatings(Carbon nitride is a new material whose hardness is comparable to the diamond and also has a very high thermal stability.) (3) to produce cesiated amorphous diamond thin film coated Si surface exhibiting negative electron affinity characteristics. In this presentation, the principles of solid state cesium ion beam sputter deposition and several applications of negative metal ion source will be introduced.

• 한국정보통신학회논문지
• /
• 제9권4호
• /
• pp.871-876
• /
• 2005

본 논문에서는 유비쿼터스 센서 네트워크의 핵심인 RFID에 대한 응용기술을 개발하기 위하여 대상을 놀이공원으로 잡고, 공원에 출입하는 입장객들이 각 놀이 기구에 입장한 시간과 나가는 시간을 자동으로 입력 받아서 데이터베이스에 저장한 후에 전체적인 놀이공원 인원 배치에 관한 합리적인 지시, 명령을 도출하도록 하는 프로그램을 개발하는 시스템을 개발하였다. 이를 위하여 본 논문에서는 13.25Mhz 대의 RFID 센서와 마이크로소프트사의 MSSQL 데이터베이스와 IIS 웹서버를 기반으로 하는 시스템 구축을 수행하였다. 이를 위하여 SkyeTek사의 RFID 리더를 기반으로 시스템을 구축하여 호스트 PC와 연동 프로그램을 C++ 언어를 이용하여 개발하였고, ASP.NET과 $C\#$을 이용한 웹사이트 구축하였으며, RFID 리더의 태그 인식 범위를 확장하기 위한 외장형 안테나를 제작한 $30\~40$ cm 수준의 인식 거리를 보장하는 동판형 안테나를 개발하였으며, 시스템에 적용한 결과 성능의 우수함을 확인하였다.

• 한국수산과학회지
• /
• 제31권6호
• /
• pp.835-841
• /
• 1998

고추냉이를 이용하여 식품의 천연보존료로 개발하기 위한 기초자료를 제공할 목적으로 우리 나라에서 재배한 고추냉이와 일본의 고추냉이의 뿌리에서 증류수로 추출한 성분의 항균활성과 항변이원성 활성에 대하여 실험한 결과를 요약하면 다음과 같다. 고추냉이 뿌리의 항균활성은 증류수 추출물이 에탄을 추출물보다 더 강하였으며 세균보다는 곰팡이에게 더 강한 항균활성을 나타내었다. 세균의 경우 그램양성균보다는 그램음성균에게 항균활성이 강하였다. 한편 일본산과 한국산 고추냉이 뿌리의 항균활성에는 유의할 만한 차이는 없었다. 4종류의 세균 중 V. parahaemolyticus에 대한 MIC가 6,000ppm으로 가장 낮았으며, E. coli와 Staphy. aureus 및 B. subtilis의 MIC는 12,000ppm으로 같았다. MBC 역시 V. parahaemolyticus가 12,000ppm으로 가장 낮았다. 고추냉이 뿌리 추출물의 항변이원활성은 첨가량에 비례하여 증가하였으며 MeIQx보다 Trp-P-1에 대하여 항변이활성이 강한 것으로 나타났다.

• 대한미생물학회지
• /
• 제18권1호
• /
• pp.53-58
• /
• 1983

Enterotoxigenic E. coli is one of causative agents of the infantile diarrhea and traveler's diarrhea. A modified infant mouse assay(IMA) was developed for the detection of heat stable enterotoxin (ST) of E. coli isolated from diarrheal and control infants and assay system was established with using enterotoxin producing reference strains. The supernatant of the 24 hour-shaking culture of E. coli in Casamino Acid Yeast Extract Salt Broth(CYES-2) was ingested orally into the 2-4 day old ICR mice. After the mice were kept at $25^{\circ}C$ for 4 hours, they were sacrificed and the gut weight body weight ratio(GW/BW) was taken as the index of fluid accumulation induced by heat stable enterotoxin of E. coli. The results obtained were as follows; 1. The GW/BW responses of IMA tested with enterotoxin reference strains of E. coli(E. coli O148H28:$ST^+LT^+$, E. coli $O78H^-:ST^+LT^+$, E. coli O15H11:$ST^-LT^+$, E. coli O1H7:$ST^-LT^-$) appeared ta be ST dose-dependent, and not LT-dependent. From the dose-response curve, $25{\mu}l$ of culture supernatant was determined as test amount of the IMA. 2. Frequency distribution of IMA result from 643 strain of E. coli showed normal distribution at low GW/BW ratio and dispersed pattern at high GW/BW ratio. The GW/BW ratios of $0.056{\pm}0.004(mean{\pm}SD)$ of normal distribution which distributed from 0.044 to 0.068(P<0.01) was considered as ST negative. Thus the GW/BW ratio above 0.069 could be regarded as ST positive.

• 한국농림기상학회지
• /
• 제7권4호
• /
• pp.289-295
• /
• 2005

본 논문은 경북지역 소나무의 연륜생장에 대하여 연륜생태학적 해석을 통하여 월별 기후인자(월별평균온도, 월별강수량)간의 관계를 기초통계량 및 상관분석을 실시하였다. 실측된 연륜생장곡선은 수령 및 비기후적인 요소를 제거하기 위하여 음지수생장곡선을 이용하여 표준화를 실시하였다. 경북지역 소나무 연륜생장과 기후인자간의 관계에서 당해연도 $2\~4$월까지의 월별평균온도가 정의 상관으로 영향을 미치고 있으며. 월별 강수의 경우 당해연도 $1\~3$월의 강수가 연륜생장에 정의 상관으로 영향을 미치는 것으로 나타났다. 이러한 결과로 미루어 경북지역 소나무의 연륜생장에 있어서 휴면기가 끝나고 생장이 시작되는 시점에서의 기후인자가 생장을 촉진시키는 요인인 것으로 판단된다.