• Korean journal of applied entomology
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• v.53 no.3
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• pp.247-260
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• 2014

To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3'terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ${\leq}0.005$ and ${\geq}0.080$, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

• Journal of Animal Environmental Science
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• v.14 no.3
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• pp.175-182
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• 2008

This research was conducted to examine proper manure treatment methods for a high-rise swine barn with results of the fact-finding survey on manure treatment methods used in high-rise swine barns and sawdust swine barns In Korea. The average temperatures on bedding of control, T1, and T2 were between $25.3{\sim}25.4^{\circ}C$ with little difference. Air flow rate of T2 was slower than those of control and T1. Dust generation in control was 54 cpm which was higher than T1 and T2. The average ammonia concentration in control was highest showing 6.0 ppm, ranged from 0.5 to 14.5 ppm, with statistical difference (p<0.05), compared to T1 (2.8 ppm) and T2 (2.6 ppm). Sawdust spreading used in control and T1 decreased ammonia concentration. T1 showed the lowest concentration of $CO_2$. Water contents of control and T2 were close to the proper water content, 65%. T1 had the highest water content and pH, and the lowest organic matter and C/N ratio. The volumes of sawdust spread per head were 0.26 and $0.27m^3$ in control and T2, respectively, which was less than T1. Operating cost such as an electricity bill for blowers was cheap in T2. Hence, T2 bedding sawdust up to 10 cm thick and periodically spreading additional sawdust from second story was recommended.

• Bulletin of the Korean Chemical Society
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• v.19 no.4
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• pp.444-449
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• 1998

The kinetics of the formation of triplex $poly(dA){\cdot}[poly(dT)]_2$ from poly(dA)·poly(dT) and poly(dT) is examined by various optical spectroscopic methods, including absorption, circular and linear dichroism (LD) spectroscopy. In the pseudo first order condition, where the poly(dT) concentration is kept lower than that of duplex, the association of the poly(dT) is enhanced by the presence of ethidium; the rate constant is proportional to the amount of ethidium in the mixture. When the concentration of the duplex and the single strand is the same, a spectral change is explained by double exponential curves, indicating that at least two steps are involved, the fast association and slow rearrangement steps. In contrast to the pseudo first order kinetics, the association step in an equimolar condition is not affected by the presence of ethidium. In the rearrangement step, the magnitude of LD decreases with an increase in ethidium concentration, suggesting that the bending of polynucleotide around the intercalation site occurs in the rearrangement step.

• Journal of Animal Science and Technology
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• v.44 no.5
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• pp.531-540
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• 2002

This study was conducted to examine the effect of Hwangto, Illite, and any other disease resistant materials as dietary supplements on the growth performance and immunity for growing period with 30 Hanwoo male calves weaned 75days in age. Feeding trial was conducted with 6 treatments(five heads/treatment), which were T1(Control), T2(Control + 2% Hwangto), T3(Control + 2% Illite), T4(Control + 0.04% Oligosacharides), T5(Control + 2% Charcoal powder) and T6(Control + 0.1% Chromium picolinate) for 120 days from three to seven months in age. The results obtained are summarized as follows; During the experimental period, average daily gains were 0.82 to 0.92kg, and were high in the order of T3, T6, T4, T5, T2 and T1. Especially the growth rate of calves for growing period was higher in Illite, chromium-picolinate and oligo- sacharides feeding groups than in any other groups. Average daily intakes and intake ratio to body weight of concentrates for 120days were 3.91 to 4.15kg(average 4.03kg) and 3.10 to 3.31% (average 3.21%), respectively. TDN intakes per kilogram gains were 3.20 to 3.57kg(average 3.35kg) and were smaller in the order of T5, T3, T6, T4, T2 and T1, respectively. Density of IgG in serum of calves measured by the IgG SDID Kit was 10.2 to 11.6mg/$m\ell$, and especially increase rate of IgG for experimental period was high in T3 and T5 by 6.9 and 2.8%, respectively. But incidence of disease was not found to be different by treatments. According to the above results it may be concluded that Illite is a sort of clay minerals increased the growth rate, feed efficiency and immunity of early weaned calves for growing period, but was not in unprocessed Hwangto.

• Investigative Magnetic Resonance Imaging
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• v.25 no.3
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• pp.141-155
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• 2021

Purpose: To develop a 3D magnetic resonance fingerprinting (MRF) method for application in high resolution knee cartilage PD, T₁, T₂ mapping. Materials and Methods: A novel 3D acquisition trajectory with golden-angle rotating radial in k_xy direction and interleaved echo planar imaging (EPI) acquisition in the k_z direction was implemented in the MRF framework. A centric order was applied to the interleaved EPI acquisition to reduce Nyquist ghosting artifact due to field inhomogeneity. For the reconstruction, singular value decomposition (SVD) compression method was used to accelerate reconstruction time and conjugate gradient sensitivity-encoding (CG-SENSE) was performed to overcome low SNR of the high resolution data. Phantom experiments were performed to verify the proposed method. In vivo experiments were performed on 6 healthy volunteers and 2 early osteoarthritis (OA) patients. Results: In the phantom experiments, the T₁ and T₂ values of the proposed method were in good agreement with the spin-echo references. The results from the in vivo scans showed high quality proton density (PD), T₁, T₂ map with EPI echo train length (N_ETL = 4), acceleration factor in through plane (R_z = 5), and number of radial spokes (N_spk = 4). In patients, high T₂ values (50-60 ms) were seen in all transverse, sagittal, and coronal views and the damaged cartilage regions were in agreement with the hyper-intensity regions shown on conventional turbo spin-echo (TSE) images. Conclusion: The proposed 3D MRF method can acquire high resolution (0.5 mm³) quantitative maps in practical scan time (~ 7 min and 10 sec) with full coverage of the knee (FOV: 160 × 160 × 120 mm³).

• The Journal of Korean Medicine
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• v.26 no.4
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• pp.39-45
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• 2005

Objectives: The study investigated the efficacy of oral Korean red-ginseng (RG) on sCRP response mediated by eccentric contraction-induced muscle damage and discussed its mechanism. Methods: Nineteen healthy young subjects (aged 24.4$\pm$2.07 yr) volunteered for this double blind test. They were classified into either the RG group (N=10) or placebo (P) group (N=9) during the 10-d experimental protocol. Blood samples were collected on T1 (baseline), T1 (1h), T2 (1d), T3 (2d) and T4 (3d) after eccentric contraction-induced injury on the seventh day. Statistical analyses were conducted using nonparametric methods (p<0.05). Results: The RG group increased sCRP more than P group and didn't increase soreness peak at T2, not significant. There were no correlations between soreness intensity and sCRP. Oral RG had little efficacy for reducing sCRP at the muscle damage-mediated acute phase; rather, it increased because of its proinflammatory cytokine production. Conclusions: Oral RG could stimulate proinflammatory cytokine production, and occasionally bal-pyo-beob could be helpful for the efficient recovery of muscle injury.

• Journal of Korean Medicine for Obesity Research
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• v.20 no.2
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• pp.78-87
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• 2020

Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal medicine in 3T3-L1 preadipocyte cells. Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 ㎍/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 ㎍/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses. Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α. Conclusions : These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.12
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• pp.1846-1854
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• 2022

The sophistication and diversification of mission equipment for surveillance and reconnaissance is leading to a demand for large-capacity public data links. Overseas, a T4 class(274Mbps) common data link was applied to the Global hwak, a high-altitude unmanned aerial vehicle, and various research and development are being conducted in Korea. In this paper, we propose a structure in which pilot is additionally applied to improve SNR performance while minimizing data transmission rate loss in the DVB-S2 frame structure, which is a european satellite broadcasting standard, for high-capacity transmission of T4 class or higher in the common data link. For the performance evaluation of the proposed structure, the performance of the DVB-S2 was compared and analyzed by simulating the UAV data link channel environment. As a result of simulation, 0.15% of transmission rate loss occurred at T4 class transmission rate compared to DVB-S2 in the proposed structure, but improved SNR reception performance of 0.2~0.3dB was confirmed in the UAV channel environment.

• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.507-512
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• 2018

Three natural Pinus thunbergii stands in southern Korea were studied to investigate stem density, biomass expansion factors, allometric equations and stand biomass. Stand ages of stand 1, 2 and 3 were 15, 29 and 45 years old, respectively. Three $10m{\times}10m$ plots were set up, five sample trees were cut and roots of three sample trees were excavated for dimension analysis in each stand. Stem density of stand 1, 2 and 3 were $0.450g/cm^3$, $0.440/cm^3$ and $0.457g/cm^3$, respectively, and there was no significant difference among the three stands. Biomass expansion factors of above-ground and total tree decreased with increasing stand age. Above-ground biomass expansion factor of stand 1 was significantly higher than those of stand 2 and 3, and total tree biomass expansion factor of stand 1 was significantly higher than that of stand 3. Allometric equations were developed for the 15 sample trees of the three stands based on D or $D^2H$. Above-ground biomass of stand 1, 2 and 3 were 50.72t/ha, 89.92t/ha, 194.07t/ha, respectively, and total tree biomass of stand 1, 2 and 3 were 61.62t/ha, 113.12t/ha, 248.36t/ha, respetively.

• Bulletin of the Korean Chemical Society
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• v.13 no.1
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• pp.11-17
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• 1992

Vibration-to-vibration energy transfer probabilities for $HF(v=n)+H_2(v=0){\to}HF(v=n-1)+H_2(v=1)$ and $DF(v=n)+D_2(v=0){\to}DF(v=n-1)+D_2(v=1)$ including both the vibration-to-vibration and translation (V-V, T) and vibration-to-vibration and rotation (V-V, R) energy transfer paths have been calculated semiclassically using a simplified collision model and Morse-type intermolecular interaction potential. The calculated results are in reasonably good agreement with those obtained by experimental studies. They also show that the transition processes for $HF(v=1-3)+H_2(v=0){\to}HF(v=0-2)+H_2(v=1)$ and $DF(v=1,\;4)+D_2(v=0){\to}DF(v=0,\;3)+D_2(v=1)$ are strongly dependent on the V-V, T path at low temperature but occur predominantly via the V-V, R path with rising temperature. The vibration-to-vibration energy transfer for $HF(v=4)+H_2(v=0){\to}HF(v=3)+H_2(v=1)$ and $DF(v=2-3)+D_2(v=0){\to}DF(v=1-2)+D_2(v=1)$ occur predominantly via V-V, R path and V-V, T path through whole temperatures, respectively.