• Title/Summary/Keyword: T-type plate

Search Result 197, Processing Time 0.023 seconds

Tissue engineering of dental pulp on type I collagen

  • Lee, Gwang-Hee;Huh, Sung-Yoon;Park, Sang-Hyuk
    • Restorative Dentistry and Endodontics
    • /
    • v.29 no.4
    • /
    • pp.370-377
    • /
    • 2004
  • The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 ($1{\;}{\times}{\;}10^5{\;}cells/ml/well$) were cultured at 12-well plate at $37^{\circ}C$ for 14 days. Standardized photographs were taken with digital camera during 14 days and the diameter of the contracted collagen gel matrix was measured and statistically analyzed with student t-test. As one of the pulp tissue engineering, normal human dental pulp tissue and collagen gel matrix cultured with dental pulp cells for 14 days were fixed and stained with Hematoxyline & Eosin. According to this study, the results were as follows: 1. The contraction of collagen gel matrix cultured with pulp cells for 14 days was significantly higher than other fibroblasts (gingiva, skin) (p < 0.05), 2. The diameter of collagen gel matrix cultured with pulp cells was reduced to 70.4% after 7 days, and 57.1% after 14 days. 3. The collagen gel without any cells did not contract, whereas the collagen gel cultured with gingiva and skin showed mild contraction after 14 days (88.1% and 87.6% respectively). 4. The contraction of the collagen gel cultured with NIH 3T3 cells after 14 days was higher than those cultured with gingival and skin fibroblasts, but it was not statistically significant (72.1%, p > 0.05). 5. The collagen gel matrix cultured with pulp cells for 14 days showed similar shape with native pulp tissue without blood vessels. This approach may provide a means of engineering a variety of other oral tissue as well and these cell behaviors may provide information needed to establish pulp tissue engineering protocols.

The Clinical usefulness of the tangential projection view of Radius groove after Distal Radius Fracture operation using the T-type Plat (전방 잠금형 금속판을 이용한 요골 원위부 골절수술 후 요골구 접선방향 촬영법의 임상적 유용성)

  • Seo, Sun-Youl;Cho, Jeong-Hee;Han, Man-Seok;Kim, Tae-Hyung;Yoo, Se-Jong;Kim, Yong-Kyun;Han, Dong-Kyoon
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.14 no.6
    • /
    • pp.2760-2766
    • /
    • 2013
  • Introduction : The projection method to check existence and nonexistence of radius groove penetration of screw after distal radius fracture operation clinical using the T-type plate and the radiation of radial groove tangential is to propose a new test. Material and Methods : On the tangential projection view of radius groove, elevation angle of distal radius is 2.5, 5, 7.5, 10 degree. and forearm and radiation detector of angle is 60, 70, 80, degree each changed. that analyzed check existence and nonexistence of radius groove penetration of screw. Results : For the image of Radial groove tangential. the case of not radius groove penetration of screw(63.95%, n=55), medial penetration of radius groove(10.47%, n=9) or lateral penetration of radius groove(25.58%, n=22) was analysis. The radius groove penetrating of screw length was a range of 0.43 ~ 2.72mm, the average was 1.06mm. the check existence and nonexistence of radius groove penetration of medial or lateral was analysis that it accurate for the image of Radial groove tangential. the radiography of radial groove is well described elevation angle of distal radius 2.5 to 7.5 degree(90% over), forearm and radiation detector of angle 70degree(80%over) Conclusion : It is elevation angle of distal radius is 5degree, forearm and radiation detector of angle 70degree recommended.

Kinetics of Converting Single Chain Urokinase Type Plasminogen Activator into Two Chain Plasminogen Activator in Cultivating HEK Cells with Low Serum Containing Medium (저혈청 배지에서 생산된 scu-PA의 tc-PA로의 전환에 관한 연구)

  • 김영남;김현구
    • KSBB Journal
    • /
    • v.9 no.1
    • /
    • pp.48-54
    • /
    • 1994
  • A modified amidolytic assay and a fibrin plate method were used to accurately measure the concentration of single chain urokinase type plasminogen activator (scu-PA) and two-chain urokinase type plasminogen activator (tc-PA) in the spent media. $1.65{\times}10^6$(viable cells/ml) of maximum cell density and 1670(IU/ml) of scu-PA concentration were obtained in 1% serum containing medium. The overall conversion ratio from scu-PA to tc-PA was less than 10%. In the results of batch cultivation in a spinner vessel, $4.43{\times}10^6(total cells/ml)$ of maximum cell density and 1560(IU/ml) of scu-PA concentration was observed. The maximum scu-PA concentration and specific scu-PA Productivity were obtained in 1760(IU/ml) and $3.13{\times}10^{-4}(IU/cell)$, respectively, from perfusion cultivation. The conveysion ratios from batch, fed-batch and perfusion cultivations were less than 12%, which means that about 90% of scu-PA secreted from the cells can be maintained during the cultivations.

  • PDF

Effects of Addition of Mugwort and Pine Needle Extracts on Shelf-life in Emulsified Sausage during Cold Storage (쑥과 솔잎 추출물을 첨가한 유화형 소시지의 냉장 저장 중 소시지의 저장성에 미치는 영향)

  • Kim, Young-Jik;HwangBo, Soon
    • Journal of Animal Science and Technology
    • /
    • v.53 no.5
    • /
    • pp.461-467
    • /
    • 2011
  • The objective of this study was to determine the shelf-life effects and residual nitrite contents of mugwort and pine needle extracts addition in emulsified sausage during cold storage. The sausages were of five types: no mugwort and pine needle extracts added (Control), mugwort water extract added (T1), mugwort ethanol extract added (T2), pine needle water extract added (T3), and pine needle ethanol extract added (T4). Each sausage type was tested in triplicate and assigned to one of four storage periods: 0, 10, 20 and 30 days. As storage time increased, the presence of mugwort and pine needle extracts resulted in decreased pH, CIE $L^*$, and residual nitrite value, but increased TBARS (thiobarbituric acid reactive substance) values, and total plate counts (TPC). Values for pH, TBARS, residual nitrite contents and total plate counts were significantly decreased by the addition of mugwort and pine needle extracts compared to the control (P<0.05). Among all treatments, T4 was more (P<0.05) effective in delaying lipid oxidation compared to other treatment groups. In conclusion, this study demonstrates that addition of pine needle ethanol extract to emulsified sausages tended to improve antioxidant and antimicrobial effects and residual nitrite contents during storage than other treatment groups.

Construction and Calibration Test of a Transmission-type Circular Polariscope for Photoelastic Stress Measurement (광탄성 응력측정을 위한 투과형 원형편광기 제작 및 시험)

  • Baek, T.H.;Koh, S.K.;Na, E.G.;Kim, J.S.;Cha, B.S.;Cho, C.H.
    • Proceedings of the KSME Conference
    • /
    • 2001.11a
    • /
    • pp.437-441
    • /
    • 2001
  • This paper describes the construction of a circular polariscope. Generally, a circular polariscope contains four optical elements and a light source. The first element following the light source is called the polarizer. It converts the ordinary light into plane-polarized light. The second element is a quarter wave plate which converts the plane-polarized light into circularly polarized light. Following the quarter wave plate, a specimen made of transparent photoelastic material is located in a loading device. The second quarter wave plate is set and the last element is the analyzer. These polarizing elements, two quarter wave plates and two polarizing filters, were purchased from the USA. Frames and other structures for holding polarizing filters were machined and assembled to be rotated. Light box, which include four incandescent lamps and two sodium-vapor lamps, was made. In order to proof the function of the newly built polariscope, Tardy compensation test was applied to a rectangular shaped specimen made of poly-carbonate material (PSM1). The error of the fringe constant, which was measured by the newly built polariscope, was within 4.4 percent compared to the standard value of this material. It is possible to make a good quality of polariscope if accurate polarizing filters will be used.

  • PDF

Numerical Analysis of Moving Type and Static Type Electrodynamic Suspension Simulator with Superconducting Levitation Magnet (초전도 부상자석을 이용한 동적 및 정지형 반발식 자기부상 시험기의 수치해석)

  • Lee, E.R.;Bae, D.K.;Chung, Y.D.;Yoon, Y.S.;Ko, T.K.
    • Progress in Superconductivity and Cryogenics
    • /
    • v.11 no.1
    • /
    • pp.49-54
    • /
    • 2009
  • This paper presents the numerical simulation results on the moving type electrodynamic suspension (EDS) simulator and static type EDS simulator using high-Tc superconducting (HTS) levitation magnet. The levitation force of the EDS system is formed by the reaction between the moving magnet and the fixed ground conductor. The possible two ways to simulate the EDS system were simulated in this paper by using finite element method (FEM). The first way was the moving type simulator which consists of the fixed HTS magnet and the moving ground conductor. The second way was the static type simulator which consists of the fixed magnet, the fixed ground conductor and the ac current supply system. To verify the characteristics of high speed EDS system with the moving type simulator heavy, large and fast moving ground conductor is needed. The static type simulator can get the characteristics of the high speed EDS system by applying equivalent ac current to velocity, therefore it does not need large moving part. The static type EDS simulator, which can consist of an HTS magnet, the fixed ground conductor(s), an AC power supply and the measuring devices, also test the effect of the shape of the ground conductor easily. The plate type ground conductor made stronger levitation force than ring type ground conductor. Although the outer diameter 335 mm ring type ground conductor (Ring3) was larger than the outer diameter 235 mm ground conductor (Ring2), the levitation force by Ring2 was stronger than that by Ring3. From the calculation results on this paper, the consideration of the magnetic flux distribution according to the levitation height should be included in the process of the ground conductor design.

A Study on Bending Fatigue Strength of One Side Fillet Welded T-Joint by SM 490A steel (Sm 490A강으로 제작된 T형 편면용접이음재의 굽힘피로강동에 관한 연구)

  • 엄동석;강성원;이태훈;이해우;조수형
    • Journal of Welding and Joining
    • /
    • v.16 no.5
    • /
    • pp.134-141
    • /
    • 1998
  • In this study, a fillet size for bending fatigue strength of one side fillet welded T-joint, used in box type girder and other welding structure, was investigated by bending fatigue test with or without edge preparation and burn through, with variation of joint shape. As a result, the following conclusions were obtained. (1) In one side fillet welded T-joint, the larger the leg length, the greater the bending fatigue strength. The increase in bending fatigue strength. (2) One side filet welded T-joint with edge preparation showed higher bending fatigue strength than that with twofold-large leg length and without edge preparation. (3) In one side fillet welded T-joint without edge preparation, both manual welding and automatic welding were carried out with same condition. In this case, automatic welding shoed deeper penetration and more increased horizontal leg length than manual welding, so that automatic welding offers grater bending fatigue strength. (4) For one side fillet welded T-joint without edge preparation, the ratio(h/t) of the leg length (h) and the main plate thickness (t) in which toe crack can occur was 1.2 over. (5) In one side fillet welded T-joint with edge preparation, the burn through led to reduced bending fatigue strength. However, this bending fatigue strength was higher than that of one side fillet welded T-joint without edge preparation and with a larger leg length.

  • PDF

Is there any determinant for successful distraction osteogenesis of the mandible in hemifacial microsomia patients? (반안면왜소증 환자의 하악골 신장술시 치료결과의 차이에 기여하는 인자)

  • Baek Seung-Hak
    • Korean Journal of Cleft Lip And Palate
    • /
    • v.7 no.2
    • /
    • pp.77-91
    • /
    • 2004
  • 본 연구의 목적은 반안면왜소증 환자의 하악골 신장술시 초기 치아골격 특성들 중에서 치료결과의 차이에 기여하는 인자를 찾는 것이다. 치료전의 치아골격 특성, 골신장술의 효과와 그 유지상태를 관찰하기 위하여 골신장술 직전(T0), 직후(T1), 추적 2년후(T2)에 측모와 정모 두부방사선 계측사진을 촬영하여 전후방, 수직치아, 비대칭 항목들을 계측하였다. T2 시기의 계측 결과에 따라서 환자들을 1군(양호군, 10명)과 2군(불량군, 9명)으로 분류하였다. 두 군에서 각 시기와 T0-T1, T1-T2 동안의 변화량의 차이를 Mann-Whitney U test, Wilcoxon signed independent t-test, rank test, ANOVA test를 사용하여 분석하였다. pruzansky type이 골신 장술의 성공과 실패 여부와 관계가 깊게 나타났다. T0 시기에 2군은 1군에 비하여 하악골이 후방위치되었고, 하악지 고경(ramus height)이 짧았고, 하악각(gonial angle)이 컸으며, 이환측 하악지가 내측경사되었고, 이환측으로의 이부변위(chin point deviation)가 크게 나타났다. 1군에서 골신장술의 주요한 효과는 하악지 고경의 증가, 하악골의 전방위치, 하악각의 증가, articular angle의 감소에 따른 하약골의 반시계방향 회전, 이환측의 하악지 경사의 증가, 교합면경사와 이부변위의 개선으로 나타났다. 그러나 2군에서는 골신장술을 시행했을 때 1군에 비하여 하악골이 시계방향으로 회전되었고 하악지 고경의 증가량이 작게 나타났다. T2 시기에 2군에서는 하악골의 반시계방향 회전이 나타났고, 하악지 성장이 일어나지 않았으나, 1군은 반대의 경향을 보였다. 이러한 인자들이 골신장술 결과의 차이에 기여하는 것으로 생각된다.

  • PDF

Basic Study on 250KW-class IoT Electric Heater for Ship Fuel Heating (선박연료 가열용 250KW급 판형 IoT 전기히터의 기초연구)

  • Jang, Sung-Cheol
    • Journal of Internet of Things and Convergence
    • /
    • v.7 no.3
    • /
    • pp.63-68
    • /
    • 2021
  • Domestic electric heater manufacturers are focusing on technology development to improve the efficiency of existing products. The electric heater for ship fuel is large in size and has high difficulty in miniaturization/high efficiency, so there are not many companies that try to develop technology yet. The existing electric heater has a structure of low heating efficiency because the contact time with the heat exchange tube of the heating medium is very short because the flow path of the ship engine fuel is monotonous. Since it is manufactured in a tubular shape, the volume is very large regardless of the heating efficiency. As a result, the tubular electric heater device applied by overseas advanced companies has difficulty in maintaining and repairing because it is necessary to decompose all tubular heaters when a specific part of the inside is damaged and if the heat exchange tube is damaged, all the heat exchange tubes must be decomposed. In this study, a basic study on plate type electric heaters capable of heating 10 tons of ship engine fuel per hour and setting a maximum temperature of up to 150℃ was conducted through a 250kW capacity plate type electric heater.

Effects of Traditional Sauce Type and Storage Time on Quality Characteristics, Shelf-life and Flavor Compounds of Marinated Pork Cooked by Sous Vide Method

  • Kim, Yong An;Ba, Hoa Van;Hwang, Inho
    • Food Science of Animal Resources
    • /
    • v.39 no.3
    • /
    • pp.355-370
    • /
    • 2019
  • The present study aimed at evaluating effects of traditional sauce type and storage time on shelf-life and flavor compounds of marinated pork cooked by Sous-Vide method. Five different traditional sauces (Meju soy sauce, Brewed soy sauce, Fish-soy sauce, Ishiru fish sauce and Anchovy fish sauce) purchased from Asian countries were used. After marination with the sauces, polyvinylchloride film bags containing the marinated pork samples were cooked using Sous-Vide method for $55^{\circ}C$ for 5 h and $60^{\circ}C$ for 30 min, and were then stored for 8 wk at $10^{\circ}C$. Results showed that the pork samples marinated with the sauces retarded the growth of total plate counts (TPC) during storage. At $8^{th}$ wk storage, TPC counts were significantly lower in all samples marinated with the sauces compared to control (p<0.05). Lipid oxidation level was significantly lower in the T2 (Meju soy sauce) and T6 (Anchovy fish sauce) compared to those of T3 (Brewed soy sauce) and T5 (Ishiru fish sauce) or control after 8 wk storage (p>0.05). Forty volatile flavor compounds were detected from the control and marinated samples at $4^{th}$ wk of storage. The pork marinated with Anchovy fish sauce presented significantly higher amounts of importantly pleasant flavor compounds such as; pyrazines and sulfurcontaining compounds than those marinated with other remaining sauces and control. It is concluded that the marination with Anchovy fish sauce partly improved the shelf-life and increased amounts of pleasant flavor compounds of Sous-Vide cooked pork products during storage in comparison to the other remaining sauces.