• Molecules and Cells
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• v.43 no.3
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• pp.304-311
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• 2020

Methionyl-tRNA synthetase (MRS) is essential for translation. MRS mutants reduce global translation, which usually increases lifespan in various genetic models. However, we found that MRS inhibited Drosophila reduced lifespan despite of the reduced protein synthesis. Microarray analysis with MRS inhibited Drosophila revealed significant changes in inflammatory and immune response genes. Especially, the expression of anti-microbial peptides (AMPs) genes was reduced. When we measured the expression levels of AMP genes during aging, those were getting increased in the control flies but reduced in MRS inhibition flies age-dependently. Interestingly, in the germ-free condition, the maximum lifespan was increased in MRS inhibition flies compared with that of the conventional condition. These findings suggest that the lifespan of MRS inhibition flies is reduced due to the down-regulated AMPs expression in Drosophila.

• Journal of Korean Medicine for Obesity Research
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• v.22 no.1
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• pp.38-46
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• 2022

Objectives: Juknyeok (JN) is natural liquor extracted from bamboo stems (Phyllostachys bambusoides) and has been used as a traditional Korean medicine for improving vascular function, blood glucose, and treating stroke. Until now, the JN's lipid-lowering effect and underlying mechanism in adipocytes are poorly understood. The aim of this study was to scrutinize the effect of a standardized commercial JN on lipid accumulation during the differentiation of 3T3-L1 preadipocytes. Methods: Lipid and triglyceride (TG) accumulation in differentiating 3T3-L1 preadipocytes were measured by Oil Red O staining and AdipoRed assay, respectively. Cell count analysis was used to ascertain 3T3-L1 cytotoxicity. Immunoblotting and Reverse transcription polymerase chain reaction analysis were used to assess protein and messenger RNA (mRNA) expression levels in 3T3-L1 cells, respectively. Results: Treatment with JN at 25 𝜇l/ml after pH calibration with 6.35 significantly reduced lipid and TG accumulation in differentiating 3T3-L1 preadipocytes without significant cytotoxicity. On mechanistic levels, JN markedly suppressed protein expression levels of CCAAT/enhancer-binding protein (C/EBP)-𝛽 and fatty acid synthase (FAS) during the differentiation of 3T3-L1 preadipocytes. However, JN did not affect the protein expression levels of C/EBP-𝛼, peroxisome proliferator-activated receptor-𝛽/𝛾, and phosphorylation levels of signal transducer and activator of transcription-3/5 in differentiating 3T3-L1 preadipocytes. JN also reduced leptin mRNA expression levels in differentiating 3T3-L1 preadipocytes. Conclusions: JN at 25 𝜇l/ml lowers lipid accumulation and TG content in differentiating 3T3-L1 cells, mediated through the reduced expression levels of C/EBP-𝛽 and FAS.

• Bulletin of the Korean Mathematical Society
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• v.47 no.5
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• pp.997-1000
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• 2010

We prove that the reduced free product of $k\;{\times}\;k$ matrix algebras over abelian $C^*$-algebras is not the minimal tensor product of reduced free products of $k\;{\times}\;k$ matrix algebras over abelian $C^*$-algebras. It is shown that the reduced group $C^*$-algebra associated with a group having the property T of Kazhdan is not isomorphic to a reduced free product of abelian $C^*$-algebras or the minimal tensor product of such reduced free products. The infinite tensor product of reduced free products of abelian $C^*$-algebras is not isomorphic to the tensor product of a nuclear $C^*$-algebra and a reduced free product of abelian $C^*$-algebra. We discuss the freeness of free product $II_1$-factors and solidity of free product $II_1$-factors weaker than that of Ozawa. We show that the freeness in a free product is related to the existence of Cartan subalgebras in free product $II_1$-factors. Finally, we give a free product factor which is not solid in the weak sense.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1015-1019
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• 2003

A 50 day feeding trial was conducted with White Leghorn (WL) laying hens, 42 weeks old, to determine if feeding of varying levels of aflatoxin (AF), ochratoxin A (OA) or their combinations has any effect on their performance and egg quality parameters. Feeding of $T_4$, $T_7$, $T_8$, $T_9$ and $T_10$ caused significant reduction in feed intake of hens. Hen day egg productions were significantly reduced at all the levels of toxins except 0.5 ppm of AF. Maximum reduction in egg production was noticed at 2 and 4 ppm of AF and OA, respectively. Average body weight and egg weight were not affected by toxin feeding. The feed efficiency in terms of net feed efficiency and feed consumed per dozen egg produced was significantly reduced at higher levels of both the toxins and their combinations. Feed consumption for production of 1 kg egg mass remained uninfluenced due to aflatoxin feeding whereas significant increase in the value of the same was noticed at 4 ppm level of OA and combination of 1 and 2 ppm of AF and 2 and 4 ppm of OA ($T_9$ and $T_10$), respectively. Various levels of OA (1-4 ppm) and all the combination of two toxins ($T_8$, $T_9$ and $T_10$) significantly altered the shape index of eggs in laying hens. The shell thickness was significantly reduced by higher level of AF (2 ppm), OA (2 and 4 ppm) and their combination. Albumen index, Haugh Unit and yolk index remained unchanged due to incorporation of toxins in the diet. It is concluded that AF, OA either singly or in combination at higher levels could depress the performance in terms of egg production and feed efficiency significantly. The egg quality parameters i.e. shape index and shell thickness were also significantly affected.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.58-60
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• 2001

Two experiments were conducted to study the effects of nutrient level and feeding method of split diets for a.m. and p.m. on laying hen performance. In both studies, ISA Brown layers were kept in laying hen cages and treatments were represented by three or four replicates each containing 25 or 34 birds. In Experiment 1, the control group(C) was fed a conventional single diet throughout the day and split diet groups(T1, T2 and T3) were offered high energy/protein - low Ca diet and low energy/protein - high Ca diets in a.m. and p.m., respectively. In split diet groups, ME and CP consumption, and feed cost required per day and per kg egg mass were significantly reduced(P＜0.05) compared to C group, while the hen-day egg production, average egg weight and daily feed intake were not different among treatments. In the second study, C and T1 groups were fed the diets same to the C and T2 groups in Experiment 1, respectively. And T2 group was fed the diet mixed with the split diets for a.m. in mash and p.m. in pellet used in T1 group. In T1 and T2 groups, daily feed intake and average egg weight were significantly reduced(P＜0.05), while the hen-day egg production was not influenced by the feeding system. Daily ME and CP consumption, and feed cost were reduced(P＜0.05) in T1 and T2, while the ME, CP and feed cost required per kg egg mass were not different to the C group. In both study, eggshell qualities were improved(P＜0.05) by split diet feeding. It was concluded the feed and nutrients consumption, feed cost per day or per kg egg mass could be spared by introducing split diets for a.m. and p.m., and the feeding method of mixed diet of split diets was also convenient and effective for sparing feed cost and improvement of eggshell quality.

• Journal of Animal Science and Technology
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• v.52 no.1
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• pp.43-50
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• 2010

The study was carried out to investigate the effects of chitosan-adding (0.5-1.5%) on nitrite-reduced (30 ppm) and sodium lactate-reduced (0-2%) sausages to avoid using excessively sodium lacte, which is substituted for sodium nitrite, The number of 24 rats for blood properties were used in this experiment and raised for 1-4 weeks. The color of sausages showed significant differences each treatment (p<0.05) and $a^*$ (redness) had the highest value in control (nitrite 100 ppm) and $b^*$ (yellowness) had the lowest value in T3 (nitrite 30 ppm + sodium lactate 0% + chitosan 1.5%). There were not significant differences in pH (5.53-5.66) and water holding capacity (66.06-69.75%) between control and two treatments (T1, nitrite 30 ppm + sodium lactate 2% + chitosan 0.5%; T2, nitrite 30 ppm +sodium lactate 1% + chitosan 1%), but T3 had significant differences in pH (5.06) and water holding capacity (62.44%), respectively. Springiness, cohesiveness, chewness and adhesiveness in texture analysis had not significant differences between control and three treatments, but hardness and gumminess had lower values in control than in three treatments. Appearance and color in sensory evaluation had higher values in control than in T1, but texture and flavor had lower values than in three treatments. Microbial counts had not significant differences in control, T2 and T3 for 1 week, for 3 weeks, it showed the lowest value in control than in three treatments. Anti-oxidant activity (TBARS) in sausages were more effective in control (p<0.05). The body weigh gain of rat were significantly increased in three treatments and also neutral fat, total cholesterol, LDL-cholesterol were significantly decreased in three treatments. However, T1 treatment had higher blood glucose content and significantly decreased in HDL-cholesterol, compared with control, but T2 and T3 treatments showed similar results in body weight gain and blood properties. So, through the addition of chitosan, it's possible to manufacture nitrite-reduced and sodium lactate-reduced sausage which is supplemented its function.

• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2000.04a
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• pp.17-17
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• 2000

The issue of reflow profiling continues to be a complex topic. The pains often associated with profiling can be reduced greatly if certain guidelines are followed and if there is a strong understanding of the variables that can be encountered during the reflow process. This paper shall discuss the appropriate guidelines and trouble shooting methods for reflow profiling, and in particular shall focus upon the benefits of implementing the linear ramp-to-spike profile. Delta T(T) is defined as the variation of temperature found on an assembly during the reflow process. Too large of a T can result in soldering defects, so to combat T a Ramp-Soak-Spike(RSS) reflow profile often is utilized. However, when using a newer-style reflow oven, the T often is minimized or eliminated, thus, the soak zone of the reflow profile becomes an unnecessary step. Because of this, the implementation of a linear Ramp-To-Spike(RTS) reflow profile should be considered. Benefits such as reduced energy costs, reduced solder defects, increased efficiency, improved wetting, and a simplification of the reflow profile process may be experienced when using the RTS profile. Included in this paper are the suggested process parameters for setting up the RSS and RTS profiles and the chemical and metallurgical reactions that occur at each set point of these profiles. The paper concludes with a discussion and pictures of several profile-related defects. Each of these defects is described, analyzed, and instructions are given for troublshooting these defects.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.7
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• pp.1247-1253
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• 2008

A design method of a T-S fuzzy model based reduced order nonlinear unknown input observer(NUIO) is presented. The fuzzy NUIO is designed based on the parallel distributed compensation(PDC) concept. It consists of a number of the linear UIOs, each of which is designed for each local linear model in the T-S fuzzy model of a class of nonlinear systems. The fuzzy NUIO provides not only the state estimates insensitive to the unknown inputs, for example, disturbances and faults etc., but also the estimates of the unknown inputs. Therefore, It can be employed in the state feedback control and disturbance rejection control of a class of nonlinear systems with unknown disturbances. It also applied to the robust residual generation for the fault detection and isolation systems and to the design of fault tolerant control systems. As an example, the NUIO is applied to an inverted pendulum system to show the state and disturbance estimation performance and to illustrate the fuzzy reduced order NUIO design method.

• Journal of the Korean Chemical Society
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• v.45 no.4
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• pp.293-297
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• 2001

The existing vapor pressure measurements reported in the literature for parahydrogen between the triple point and the critical point have been employed to establish the constants and exponent of the following equation in the form of reduced vapor pressure and reduced temperature: ln $lnP_r=2.64-{\frac{2.75}{T_r}}+1.48129lnT_r+0.11T^5_r$Only the normal boiling point ($T_b$= 20.268K), the critical pressure ($P_c$= 1292.81 kPa), and the critical temperature ($T_c$= 32.976K) are necessary to calculate the vapor pressure for an overall average deviation of 0.21% for 153 experimental vapor pressure data.

• Journal of applied mathematics & informatics
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• v.33 no.5_6
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• pp.559-578
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• 2015

Human Immune Deficiency Virus (or simply HIV) induces a persistent infection that leads to AIDS causing death in almost every infected individual. As HIV affects the immune system directly by attacking the CD4+ T cells, to exterminate the infection, the natural immune system produces virus-specific cytotoxic T lymphocytes(CTLs) that kills the infected CD4+ T cells. The reduced CD4+ T cell count produce reduced amount of cytokines to stimulate the production of CTLs to fight the invaders that weakens the body immunity succeeding to AIDS. In this paper, we introduce a mathematical model with discrete time-delay to represent this cell dynamics between CD4+ T cells and the CTLs under HIV infection. A modified functional form has been considered to describe the infection mechanism. Characteristics of the system are studied through mathematical analysis. Numerical simulations are carried out to illustrate the analytical findings.