• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.313-321
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• 2021

BACKGROUND: Ammonia is a causative substance for the fine particulate matters (PM_2.5) and generates dust through atmospheric reactions. Agricultural sector accounts for 79.3% of ammonia emissions in Korea. Urea and composted organic fertilizer (COF) are used in the soil for the purpose of supplying nutrients in grapevine orchards. This study was conducted to investigate estimates of ammonia emission and examine fruit quality from the rain proof cultivation of the 'Beni Balad', applied by urea and COF to the soil. METHODS AND RESULTS: Urea, COF1, and COF2 were applied at the rates of 119, 135, and 271 kg ha^-1 respectively. Ammonia emissionwas measured using a dynamic flow-through method. CONCLUSION(S): Ammonia emissions by urea and COF treatments to 'Beni Balad' soils under rain proof cultivation were calculated to be 2.63, 12.95, 2.05, and 3.97 kg NH₃-N ha^-1 day^-1, respectively for the control, urea, COF1, and COF2. Urea soil application increased soluble solids, firmness, and anthocyanin content in fruits at harvest, and COF1 application increased the soluble solids and anthocyanin content. For all the treatments, acidity increased in the harvested fruits.

• ALGAE
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• v.37 no.3
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• pp.227-237
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• 2022

Knowledge on the seaweeds of eastern Sorsogon in the Philippines is uneven. Not only is eastern Sorsogon among the areas of high seaweed diversity but locals there have interacted with seaweeds for ages. Despite seaweeds' assumed importance to Sorsoganon, ethnobotanical records are missing. In this study, we documented the traditional knowledge on seaweed use and determined the rate of knowledge transmission among the locals of eastern Sorsogon. Vernacular names and modes of preparation were given of the 12 identified species bearing culinary and medicinal importance. Ten species were eaten while three were used therapeutically. Based on ethnobotanical indices, Caulerpa chemnitzia ecad turbinata and Gelidiella acerosa were the most important seaweeds. The least cited was Caulerpa racemosa. Our study demonstrated that most of the seaweed resources in eastern Sorsogon remain largely untapped, as the identified ethnotaxa were only about 5% of the total seaweed species diversity reported for the area. The seaweed knowledge in eastern Sorsogon appeared to be homogenous across age groups with primary sources of knowledge biased towards female relatives (mothers and grandmothers) and to children as inheritors. Cessation of seaweed knowledge may come at a potential cost, as a significant fraction of the population did not transmit their knowledge to others. Our study furthered the interest in providing sophisticated resource management recommendations that consider the relationships of traditional and scientific knowledge of seaweed selection and use in eastern Sorsogon and beyond.

• Journal of Intelligence and Information Systems
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• v.28 no.4
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• pp.365-385
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• 2022

The easy payment service is a payment and remittance service that uses a simple authentication method. As online transactions have increased due to COVID-19, the use of an easy payment service is increasing. At the same time, electronic financial industries such as Naver Pay, Kakao Pay, and Toss are diversifying the competition structure of the easy payment market; meanwhile overseas fintech companies PayPal and Alibaba have a unique market share in their own countries, while competition is intensifying in the domestic easy payment market, as there is no unique market share. In this study, the participants in the easy payment market were classified as electronic financial companies, mobile phone manufacturers, and financial companies, and a SWOT analysis was conducted on the representative services in each industry. The analysis examined the user reviews of Google Play Store via a topic modeling analysis, and it employed positive topics as strengths and negative topics as weaknesses. In addition, topic modeling was conducted by dividing news articles into political, economic, social, and technology (PEST) articles to derive the opportunities and threats to easy payment services. Through this research, we intend to confirm the service capabilities of easy payment companies and propose a service activation strategy that allows gaining the upper hand in the market.

• BMB Reports
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• v.55 no.6
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• pp.275-280
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• 2022

The treatment of atopic dermatitis (AD) is challenging due to its complex etiology. From epidermal disruption to chronic inflammation, various cells and inflammatory pathways contribute to the progression of AD. As with immunosuppressants, general inhibition of inflammatory pathways can be effective, but this approach is not suitable for long-term treatment due to its side effects. This study aimed to identify a plant extract (PE) with anti-inflammatory effects on multiple cell types involved in AD development and provide relevant mechanistic evidence. Degranulation was measured in RBL-2H3 cells to screen 30 PEs native to South Korea. To investigate the anti-inflammatory effects of Parasenecio auriculatus var. matsumurana Nakai extract (PAE) in AD, production of cytokines and nitric oxide, activation status of FcεRI and TLR4 signaling, cell-cell junction, and cell viability were evaluated using qRT-PCR, western blotting, confocal microscopy, Griess system, and an MTT assay in RBL-2H3, HEK293, RAW264.7, and HaCaT cells. For in vivo experiments, a DNCBinduced AD mouse model was constructed, and hematoxylin and eosin, periodic acid-Schiff, toluidine blue, and F4/80-staining were performed. The chemical constituents of PAE were analyzed by HPLC-MS. By measuring the anti-degranulation effects of 30 PEs in RBL-2H3 cells, we found that Paeonia lactiflora Pall., PA, and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. show an inhibitory activity of more than 50%. Of these, PAE most dramatically and consistently suppressed cytokine expression, including IL-4, IL-9, IL-13, and TNF-α. PAE potently inhibited FcεRI signaling, which mechanistically supports its basophil-stabilizing effects, and PAE downregulated cytokines and NO production in macrophages via perturbation of toll-like receptor signaling. Moreover, PAE suppressed cytokine production in keratinocytes and upregulated the expression of tight junction molecules ZO-1 and occludin. In a DNCB-induced AD mouse model, the topical application of PAE significantly improved atopic index scores, immune cell infiltration, cytokine expression, abnormal activation of signaling molecules in FcεRI and TLR signaling, and damaged skin structure compared with dexamethasone. The anti-inflammatory effect of PAE was mainly due to integerrimine. Our findings suggest that PAE could potently inhibit multi-inflammatory cells involved in AD development, synergistically block the propagation of inflammatory responses, and thus alleviate AD symptoms.

• Journal of the Korea Society of Computer and Information
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• v.27 no.11
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• pp.173-179
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• 2022

This study was conducted to provide basic data for the development of oral health education programs by examining oral health knowledge and oral health behaviors, and by identifying factors affecting oral health knowledge and scaling experience of Chinese domestic students. From March to May, a self-reported questionnaire was administered to 194 in the G area. The data were analyzed frequency analysis and independent t-test, multiple regression analysis, logistic regression analysis by using SPSS/WIN 26.0 program. The overall average of oral health knowledge was 13.83 out of 24, and knowledge of periodontal disease and scaling was the highest at 3.75, and knowledge of oral hygiene products was the lowest at 1.38. Oral health knowledge was significantly higher in those who had scaling experience for the past year, brushed teeth for more than 3 minutes at a time, used oral hygiene products, and had oral health education experience. As a result of multiple regression analysis, oral health education experience was the most important factor on oral health knowledge. As a result of logistic regression analysis, oral health knowledge was the most influential factor on scaling experience. It is necessary to expand education on the importance of oral care and actively introduce oral health management program for Chinese domestic students.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.30-30
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• 2001

The culture of preantral follicles has important biotechnological implications through its potential to produce the large quantity of oocytes for embryo production, transgenesis research, conservation of rare breed, and a potential source of ovarian genetic material. The present study was conducted to establish the optimal conditions of in vitro culture for intact bovine preantral follicles; and to examine the developmental ability of oocytes derived from the in vitro-grown preantral follicles; and to investigate the effects of various concentrations of FSH and LH on these processes. Bovine preantral follicles (150 $\pm$ 1.2${\mu}{\textrm}{m}$), surrounded by theca cell, were isolated enzymetically and mechanically from ovarian cortical slides in Leibovitz L-15 medium containing 1 mg/$m\ell$ collagens and 0.2 mg/$m\ell$ DNase I and cultured for 25 days in the presence of different concentrations of bovine FSH and LH in $\alpha$MEM medium with insulin, transferrin, and selenite. The survival was tested by frypan Blue and Hematoxylin. The survival and growth rates of follicles were higher in FSH treatment groups than these in control (P<0.001), but there were no significant differences between the LH treatment groups and the control. In 25 days, the survival and growth rates of follicles in FSH and LH treatment group (50%, 300$\pm$1.0${\mu}{\textrm}{m}$) were higher than in FSH treatment group (40%, 244$\pm$0.5${\mu}{\textrm}{m}$) and the control group (25%, 160$\pm$ 1.0${\mu}{\textrm}{m}$). Fifty-five percent of healthy antral follicles were obtained, and 60% of the oocytes complete meiotic maturation to the metaphase II stage. Twenty-two percent of the mature oocytes underwent cleavage, and 9% developed to the blastocyst stage. In this study, in vitro-grown oocytes (111 $\pm$ $1.5mutextrm{m}$), under our culture conditions, were not equivalent in size to the in vivo-grown oocytes (130$\pm$1.3${\mu}{\textrm}{m}$). Therefore, these results suggest that bovine preantral follicles with intact theca cell can grow to the antral stage in 25days, and that oocytes from those follicles can acquire the meiotic competence and normally undergo fertilization and development to the blastocyst stage. However, the developmental capacity of in vitro-grown oocytes is presumably not comparable to those of the in vivo counterparts.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.56-56
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• 2001

The exogenous gene transfer by intracytoplasmic sperm injection (ICSI) procedure has been recently used to produce transgenic mice and pigs. Sperm-mediated DNA transfer has the potential to markedly simplify the generation of transgenic animals. This method may serve as an alternative to the pronucleus injection of DNA for the production of transgenic pigs. Therefore, in this study, we investigated the expression of transgene after co-injection of spermatozoon or sperm head with green fluorescent protein (GFP) gene into in vitro matured porcine oocytes. Spermatozoon and sperm head, that was obtained by sonication, were treated with 0.03% Triton X-100 to remove the membrane. They were preincubated with linearized pEGFP-N1 for 1 min, and then embryos cultured NCSU23 medium for 2.5 days after co-injected of sperm and DNA. We monitored expression of GFP in embryos under epifluorescent microscope. The remove of sperm membrane did not alter the developmental competence of embryos after ICSI. At 7 days following injection, the rates of blastocysts following injection of intact sperm (15.0%), and of sperm with disrupted membrane (14.2%) were higher than that following IVF (10.0%). Porcine oocytes injected with sperm which co-cultured with DNA concentration of 1, 0.1, and 0.01 ng were 60, 65.7 and 75% and 18.5, 37.4 and 22.2% for rates of cleavage and GFP expression, respectively. In vitro matured porcine oocytes injected with sperm and isolated sperm head resulted in 69 and 59.7% of cleavage rates, respectively The rates of embryo GFP expressed did not significantly different between sperm (20.4%) and sperm head (20.0%) injection. The transgenic embryos with the clusters of positive blastomeres were observed under fluorescent microscope. Most of embryos expressed GFP gene showed mosaicism. They showed GFP expression at 1/4, 2/4 and 3/4 of blastomeres at the 4-cell stage. Among these 4-cell embryos, the expression rate of 1/4 blastomere group (54.6%) was higher than the other groups (15.3-30.7%). These results indicate that membrane disrupted sperm could attach with exogenous DNA, and that this procedure may be useful to introduce foreign gene into porcine oocytes. Therefore, our data suggest that the ICSI car be a useful tool to efficiently produce transgenic pig as well as other mammals.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.1
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• pp.67-76
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• 2023

PURPOSE: The study compared the satisfaction of the exercise program in a face-to-face and non-face-to-face manner with the same exercise program. METHODS: This study was conducted through convenience sampling for 57 seniors over 65 years of age. After conducting 30 minutes of the same exercise program face-to-face on the first visit and non-face-to-face on the second visit, a satisfaction survey was conducted. The survey data were processed statistically using SPSS 26.0 (SPSS Inc, IBM, USA). An independent t-test was used to compare the type of exercise program. The Spearman's Rank Correlation was conducted to correlate the satisfaction between cognitive function and education level. RESULTS: The overall satisfaction with the face-to-face program was higher than the non-face-to-face exercise programs. All three satisfaction items in face-to-face exercise programs were higher than in non-face-to-face exercise programs (p < .05). The main source of dissatisfaction in the case of face-to-face exercise programs was having a good relationship with people for face-to-face and a 'lack of communication' in the case of non-face-to-face. CONCLUSION: The elderly showed higher satisfaction in face-to-face exercise than in non-face-to-face exercise. This study is significant in that accurate satisfaction comparison was possible by conducting the same exercise program on the same subject for a relatively short time, and through this, the development of an exercise program was suggested.

• The Korea Journal of Herbology
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• v.38 no.2
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• pp.1-7
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• 2023

Objectives : From this study, we sight to identify Anti-stress effect of Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex Methods : In order to measure the degree of functional evaluation of tension relief due to stress, it was evaluated whether the psychological indicators VAS(Visual Analog Scale) and POMS(Profile Of Mood State) were improved. Which is generally used for stress measurement and has a high frequency of clinical use. All clinical trial subjects were required to take the drug once a day at 9 ± 30 min in the morning for 2 weeks. Results : After intake, the group that Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex had a statistically significant improvement effect compared to before intake due to an improved 30.81% of the VAS scores. The POMS-T score improved 54.13%, the POMS-V score improved 117.5% and the POMS-F score improved 59%, which had a statistically significant effect compared to Placebo group (p<0.001). There is a significant difference between two group, so even considering the placebo effect, the anti stress effect was confirmed in the Chenwangbosim-Dan (Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex intake group. Conclusions : The above results showed that the Chenwangbosim-Dan(Tianwangbuxin-Dan) Extract∘Fermented Rice Extract (GABA : gamma-aminobutyric acid 30%) Complex had an anti-stress effect. Therefore, it is expected to be developed as a safe and comfortable mental care health functional food that can reduce the risk of drug abuse.

• Journal of Environmental Health Sciences
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• v.48 no.6
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• pp.291-297
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• 2022

Background: PM_2.5 and black carbon (BC) can be generated from cooking and from vehicle operation. Street vendors may be exposed to PM_2.5 and BC due to their proximity both to roads and to cooking activities. Objectives: The objectives of this study were to evaluate the PM_2.5 and BC concentrations in cooking stalls and to determine the effects of cooking activity and of types of cooking. Methods: Indoor and outdoor PM_2.5 and BC concentrations, temperature, and relative humidity were measured in 32 stalls in April and May 2022. Behavioral factors such as the presence of cooking activity and types of cooking were observed. Student's T-test was performed using the difference of indoor and outdoor PM_2.5 and BC concentrations to compare the effects of cooking activity and to compare types of cooking. Results: One-hour averages of the difference in indoor and outdoor PM_2.5 concentrations for cooking stalls and non-cooking stalls were 9.7±15.7 ㎍/m³ (n=22) and -0.5±0.4 ㎍/m³ (n=10), respectively. The difference in indoor and outdoor PM_2.5 concentrations in cooking stalls was significantly higher than in non-cooking stalls (p<0.05). The indoor PM_2.5 concentration for stalls for Chinese pancakes and teokbokki exceeded the standards for indoor air quality in South Korea (50 ㎍/m³ ). The indoor PM_2.5 concentration for Korean pancake stalls exceeded the standards for outdoor air quality in South Korea (35 ㎍/m³ for 24 hours). Conclusions: The PM_2.5 concentrations in stalls with cooking activity was significantly higher than those in stalls without cooking activity. Some stalls with certain types of foods exceeded standards for indoor and outdoor air quality in South Korea. Better management of indoor air quality in stalls with cooking activities is necessary.