• 대한수학회보
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• 제35권2호
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• pp.363-374
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• 1998

We investigate relationships of E-depths and T-codepths of modules in s short exact exact sequence. We give E-depths and T-codepths of some modules.

• 헤리티지:역사와 과학
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• 제52권2호
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• pp.220-239
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• 2019

조선통폐지인은 "경국대전" "국폐조(國幣條)"의 예에 따라, 1필 내지 반 필의 포(布) 양단에 찍은 인신(印信)으로 포를 포폐(布幣)로 유통키 위한 용도로 사용되었다. 이때 발행된 포폐는 매매를 위한 화폐 내지, 죄를 면제받기 위한 수속(收贖)과 노역을 대신한 신공(身貢) 등의 용도로 사용되었으며, 포에 조선통폐지인을 찍어 그중 20분의 1의 세(稅)를 수납한 조세 용도로 활용되기도 하였다. 조선통폐지인은 조선 초 화폐사 및 인신(印信) 자료 중 하나이며, 조선통폐지인 압인(押印)이 조세 수단이었던 까닭에 조세제도 연구자료 중 하나임에도 이에 대한 연구가 활발히 진행되지 않았다. 이에 이전 연구자에 의해 언급된 자료뿐만이 아닌, "전록통고"와 "대전후속록", "정헌쇄록", 몇몇 지리지(地理志)에 등장하는 관련 내용을 바탕으로 포괄적인 연구를 행하였다.조선 태종 1년(1401) 정5승포에 인(印)을 찍어 "조선포화"를 유통하자는 유관의 상소를 착안해 조선통폐지인이 제작된 것임을 말하였으며, 매매를 위한 화폐 내지 수속(收贖)과 신공(身貢), 조세 수단뿐만이 아닌, 선척의 화매가 용도로 조선통폐지인을 찍은 포폐가 사용되었음을 기술하였다. 또한 중종 10년(1515) "저화행용절목" 방침에따라 1516년 3월 이후 포폐 사용이 금지된 후에도 만력 26년(1598) 지방 공문서의 관인으로 조선통폐지인이 사용된 예와, 영조 당시 포보가포(砲保價布)라는 명칭으로 군포(軍布) 제작의 예로 사용되었으며, 1779년 기록을 통해 무역에 따른 납세수단으로 조선통폐지인이 사용된 예가 있음을 서술하였다. "정헌쇄록"기록을 통해 대략 330여과의 조선통폐지인이 유통되었을 것이며, 1940년 간행된 "조선화폐고"에 조선통폐지인 인영(印影)이 실려 있음에도 조선통폐지인이 답인(踏印)된 포폐 내지 조선통폐지인 실물은 발견된 바가 없다. 그런데 최근 원각사 소장 유물 중 조선통폐지인이 발견되었으며, 1775년 작성된 직지사 소장 고문서에 압인(押印)된 조선통폐지인 인영이 발견되기도 하였다. 이에 직지사 고문서 및 "탑좌종정지"에 첨부된 인영, "조선화폐고"에 실린 인영과 원각사 소장 조선통폐지인을 비교해 보았던바, 위 문서에 압인된 인영은 원각사 소장 조선 통폐지인과 동일한 형태임을 확인할 수 있었다. 또한 전체적인 형태 역시 "대록지"내지 "이재난고"에 묘사된 모습과 비견되는 형태를 보임을 알 수 있었다.이에 원각사 소장 조선통폐지인은 15세기에 주조된 인신(印信)의 실물로, 조선의 화폐사 및 조세제도와 인신(印信) 연구의 중요 유물로 추후 다양한 측면의 연구가 필요할 것이라 하겠다.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.457-462
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• 2002

Low Electromagnetic Field (EMF) intensity in the range of $1{\mu}T\;to\;10{\mu}T$(Tesla) was found to enhance the growth of CHO cells and the production of tPA in batch and perfusion cultivations. At $1{\mu}T\;intensity,\;1.3{\times}10^7$ viable cells/ml of maximum cell density and 80 mg/l of maximum tPA production were obtained in batch cultivation, compared to $2.8{\times}10^6$ viable cells/ml and 59 mg tPA/1 in unexposed case (control). A similar trend was observed in the perfusion process, where it was possible to obtain $1.2{\times}10^7$ viable cells/ml of maximum cell density and 81 mg tPA/l of maximum tPA production by more than 80 days of cultivation. However, there was not much difference between $1{\mu}T\;and\;10{\mu}T$ in perfusion cultivation, possibly due to better environmental growth conditions being maintained by continuous feeding of fresh medium into the reactor. On the contrary, both cell growth and tPA production were severely inhibited at higher than 1 mT intensity, showing no growth at 10 mT exposure. Specific growth rate was linearly correlated to specific tPA production rate at $1{\mu}T$EMF intensity, which represents a partially growth-related relationship. It was also found that a large amount of $Ca^2+$ was released at low EMF intensity, even though the cell growth was not much affected. Low EMF intensity significantly improved both cell growth and tPA production, and tPA production seemed to be more affected than the cell growth, possibly due to the changes of cell membrane characteristics. It can be concluded that the elaboration of EMF intensity less than $10{\mu}T$ could improve cell growth and tPA production, but mainly tPA secretion through batch or perfusion process in a bioreactor.

• 수산경영론집
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• 제40권3호
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• pp.169-187
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• 2009

This study analyzes the long and short term effect of exchange rate on the import of Korea's fisheries focussed on main fisheries imported from China. The estimation models consist of the following contents. The first model consists of one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{CHO}$) and three independent variables-${RP_t}^{CHO}$, $EXC_t$ and $GDP_t$. The second one-one dependent variable-import quantity of fisheries imported from China(${JMQ_t}^{NAG})$ and three independent variables-${RP_t}^{NAG}$, $EX_t$ and $GDP_t$. the third one-one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{AH}$) and three independent variables-${RP_t}^{AH}$, $EX_t$ and $GDP_t$. the forth one-one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{KO}$) and three independent variables-${RP_t}^{KO)$, $EX_t$ and $GDP_t$. the last one is made up of one dependent variable-import quantity of fisheries imported from China(${IMQ_t}^{GAL}$) and three independent variables-, ${RP_t}^{GAL}$, $EX_t$ and $GDP_t$. and. The estimation results show that exchange rate of the independent variables are statistically significant in only the first model. The figure is elastic. Especially, the effect of exchange rate in first model is grater than that of the. However, the effect of exchange rate, one of independent variables in the ECM, is not statistically significant.

• 대한수학회논문집
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• 제37권3호
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• pp.749-763
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• 2022

Let C[0, T] denote an analogue of Weiner space, the space of real-valued continuous on [0, T]. In this paper, we investigate the translation of time interval [0, T] defining the analogue of Winer space C[0, T]. As applications of the result, we derive various relationships between the analogue of Wiener space and its product spaces. Finally, we express the analogue of Wiener measures on C[0, T] as the analogue of Wiener measures on C[0, s] and C[s, T] with 0 < s < T.

• Journal of Microbiology and Biotechnology
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• 제23권8호
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• pp.1116-1122
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• 2013

Cell line development is the most critical and also the most time-consuming step in the production of recombinant therapeutic proteins. In this regard, a variety of vector and cell engineering strategies have been developed for generating high-producing mammalian cells; however, the cell line engineering approach seems to show various results on different recombinant protein producer cells. In order to improve the secretory capacity of a recombinant tissue plasminogen activator (t-PA)-producing Chinese hamster ovary (CHO) cell line, we developed cell line engineering approaches based on the ceramide transfer protein (CERT) and X-box binding protein 1 (XBP1) genes. For this purpose, CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s were overexpressed in a recombinant t-PA-producing CHO cell line. Overexpression of CERT S132A increased the specific productivity of t-PA-producing CHO cells up to 35%. In contrast, the heterologous expression of XBP1s did not affect the t-PA expression rate. Our results suggest that CERT-S132A-based secretion engineering could be an effective strategy for enhancing recombinant t-PA production in CHO cells.

• 대한방사선기술학회지:방사선기술과학
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• 제26권2호
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• pp.57-61
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• 2003

두정부 백질 물질 NAA, Choline(Cho), Creatine(Cr), choline chloride, glutamin 등으로 자체 제작한 phantom을 이용하여 고 자장(4.7T)과 중자장(1.5T)에서의 신호강도 대 잡음비, 스펙트럼 분해능을 알아 볼 수 있는 선폭과 $T_2$, 그리고 $T_E$값의 변화에 따른 각 대사물질의 변화별 스펙트럼 등을 구하여 이론적인 정보와 실제적인 정보와의 차이를 알아보고자 한다. 이용된 기기는 Bruker Biospec 4.7T와 1.5T GE SIGNA를 이용하여 MRS의 결과를 얻었다. 관심영역에서 얻은 정보에서 분광 peak의 면적을 구하였다. 통계처리는 Microsoft사의 Excel program내에 있는 통계 package를 이용하였다. 중자장(1.5T)과 고자장(4.7T)에서의 각각의 대사물질의 스펙트럼을 얻어 본 결과 자장의 균일도와 SNR과 $T_2$값의 차이가 이론적인 값보다는 직선성을 보이지 않았다. 1.5T에서의 선폭은 Cho, Cr, NAA 순서로 $5.30{\pm}1.07,\;4.81{\pm}0.14,\;5.49{\pm}0$, 이에 반해 4.7T에서는 $9.14{\pm}0.55,\;8.87{\pm}0.67,\;9.65{\pm}0.56$ Hz 값이 나왔다. 평균 SNR은 NAA의 물질의 경우를 3회 측정하였는데 그 평균치는 63%의 증가를 보였다. $T_E$ 변화로 스펙트럼을 분석했을 때 $T_E=60ms$일 때 가장 SNR이 좋은 값을 보였고 $T_E=135ms$일 때 가장 낮은 값을 나타나 보였다. 두정부 백질 물질 Phantom을 제작하여 자장의 강도 즉 중자장(1.5T)과 고자장(4.7T)에 따라 분석하게 되었는데 고자장의 영역으로 갈수록 분해능, SNR은 좋아져 보이나 자장의 세기가 커질수록 이론적인 수치보다는 상당히 적은 증가를 보이고 있다는 것을 이 연구를 통해 알게 되었다.

• 한국동물생명공학회지
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• 제35권2호
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• pp.155-162
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• 2020

Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC₅₀ values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

• International Journal of Internet, Broadcasting and Communication
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• 제15권4호
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• pp.250-260
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• 2023

In this paper, we designed a IoT(Internet of Things) sensor block embedded lightweight house deck structures that can be implemented using Carbon Fiber Reinforced Polymer(CFRP). Deck-Sensor interconnection interface block via IoT connectivity Hub that can mount external environmental sensors such as fire sensors on the Deck body itself was also proposed. Additionally we described the configuration of devices for data acquisition and analysis based on IoT environmental detection sensors that can be commonly installed and used on these deck bodies. On the other hand, received sensing data based monitoring user interface(UI) also developed and used for sensing data analysis for remote monitoring center. Through the implementation of such IoT-based sensor data transmission and collection analysis devices and UI software, this paper confirmed the availability of CFRP based lightweight House deck structure and possibility of CFRP deck-based IoT sensor data networking and analysis functions.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1342-1351
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• 2008

The feasibility of the use of Flp-mediated cassette exchange in the development of a CHO cell line, which produces erythropoietin (EPO) stably and largely, was investigated. A stable, high enhanced green fluorescence protein (EGFP)-producing clone was screened by extensive flow cytometric analysis. An EPO expression unit was targeted into the premarked locus of the stable parental clone by Flp-mediated cassette exchange and a correctly targeted clone (FC28T7) was obtained. The EPO production of FC28T7 was proven to be stable in long-term culture. Furthermore, the Flp-mediated cassette exchange did not alter the stable parental clone's characteristics concerning transgene expression level and stability. Taken together, the data obtained here indicated that the establishment of CHO cell lines stably producing a desired protein is achievable using Flp-mediated cassette exchange.