• Korean Journal of Microbiology
• /
• v.24 no.2
• /
• pp.79-85
• /
• 1986

We have determined the sequence of $tRNA^{Arg}$ of A. nidulans partially by enzymatic rapid RNA sequencing technique. The sequence was 5'GGCCGGCUGGCCCAAXUGGCAAGGXUCUGAXUACGAAXCAGGAGAUUGCACXXXXXGAGCXXUXXGUCGGUCACCA3' The cloverleaf structure was made from above data. As a result, the anticodon sequence was identified as ACG. This result was confirmed with charging test. The complete sequence was proposed by supplementing the DNA sequence to and by assigning the position of minor bases to this RNA sequence.

• Journal of The Korean Institute of Defense Technology
• /
• v.5 no.2
• /
• pp.1-9
• /
• 2023

The purpose of this study is to derive the need to improve the test and evaluation(T&E) performance system of the weapon systems to which advanced science and technology is applied, evaluate priorities, and present development plans. T&E of Al-based weapon systems through a literature research and case analysis on changes in the T&E environment for weapon system, 11 detailed evaluation items were derived from the in terms of the T&E system, structure, and technology. Al-based weapon system test evaluation should be performed in parallel with data-based performance evaluation and actual T&E, and performance measurement using a separate T&E data set is required for AI models performance evaluation. As a result of analyzing the importance of T&E through AHP analysis, the order of T&E system-technology-structure was evaluated, and the priority of detailed evaluation items was evaluated in the order of T&E result judgment-T&E organization and expert training-scientific T&E. For evaluation items with high priority, measures to improve the T&E performance system were presented.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2002.10a
• /
• pp.15-21
• /
• 2002

Terminal-restriction fragment length polymorphism (T-RFLP) analysis has been optimized by using in vitro model community composed of genomic DNAs of known bacterial strains and has been applied to assess the bacterial community structure in marine sediments. The specific fluorescence-labeled terminal restriction fragments (T-RFs) between 39 and 839 base long specifying each strain were precisely measured for known bacterial strains. The addition of a co-solvent (dimethylsulfoxide or glycerol) into PCR reactions has reduced differential PCR amplification. Comparative bacterial community structure was investigated for pristine and polluted sediments. A complex T-RFLP pattern showing complex bacterial community structure was obtained in the pristine sediment, whereas simple T-RFLP pattern (low bacterial diversity) was shown in polluted sediments where caged aquaculture has been conducted for several years. The results of T-RFLP analysis were compared with that of cloning and sequencing 16S rDNA clones from the same sediments. Sequence analysis of 16S rDNA clones (72) of the pristine sediment revealed a diverse collection of lineages, largely of the class Proteobacteria ($6\%$ alpha subdivision, $46\%$ gamma subdivision, $13\%$ delta subdivision, and $3\%$ epsilon subdivision), Nitrospina $(8\%)$, high G+C gram positive $(8\%)$, Verrucomicrobia $(7\%)$, and Planctomycetes $(6\%)$. In the contaminated sediments, 17 $(59\%)$ of the 16S rDNA clones (29) were related to Campylobacter and symbiont of Rimicaris exoculata belonging to epsilon subdivision of Proteobacteria. The results obtained indicated that T-RFLP analysis is a rapid and precise technique for comparative bacterial community analysis.

• ETRI Journal
• /
• v.40 no.6
• /
• pp.745-758
• /
• 2018

A wide range of studies in various disciplines has focused on the Internet of Things (IoT) and cyber-physical systems (CPS). However, it is necessary to summarize the current status and to establish future directions because each study has its own individual goals independent of the completion of all IoT applications. The absence of a comprehensive understanding of IoT and CPS has disrupted an efficient resource allocation. To assess changes in the knowledge structure and emerging technologies, this study explores the dynamic research trends in IoT by analyzing bibliographic data. We retrieved 54,237 keywords in 12,600 IoT studies from the Scopus database, and conducted keyword frequency, co-occurrence, and growth-rate analyses. The analysis results reveal how IoT technologies have been developed and how they are connected to each other. We also show that such technologies have diverged and converged simultaneously, and that the emerging keywords of trust, smart home, cloud, authentication, context-aware, and big data have been extracted. We also unveil that the CPS is directly involved in network, security, management, cloud, big data, system, industry, architecture, and the Internet.

• Fisheries and Aquatic Sciences
• /
• v.25 no.3
• /
• pp.158-166
• /
• 2022

The complete mitochondrial genome of Tremoctopus violaceus was sequenced to analyze its organization and phylogenetic status within the order Octopoda. The mitochondrial genome of T. violaceus had a structure and organization similar to that of other Octopoda. The content of the nucleotides A, C, G, and T was 31.68 %, 7.71 %, 20.02 %, and 40.58 %, respectively. All protein-coding genes (PCG) began with the ATG codon, excluding ND4 and ATP6, which began with ATC and ATT, respectively, and terminated with TAG, TAA, TA, or T. Codons for isoleucine were the most used codons, whereas those for arginine were used the least. Two extra tRNAs, trnN and trnL, were found in the control region. These tRNAs have a D-armless structure. The control region had excess A + T content (83.16 %) and a stem-loop structure with two elements, which is reported for the first time in Octopoda by our study. Bayesian inference using 13 PCG revealed that Octopus and Octopodidae were polyphyletic, and that Tremoctopodidae diverged relatively earlier within Octopoda. The mitochondrial genome of T. violaceus and its characteristics may help to understand the evolutionary history of Octopoda and establish a marine biodiversity conservation strategy.

• The KIPS Transactions:PartD
• /
• v.11D no.7 s.96
• /
• pp.1357-1366
• /
• 2004

As the importance or XML is increasing to manage information and exchange data efficiently in the web, there are on going works about structural integration and retrieval. The XML. document with the defined structure can retrieve the structure through the DTD or XML schema, but the existing method can't apply to XML. documents which haven't the structure information. Therefore. in this paper we propose a new clus-tering technique at a basic research which make it possible to retrieve structure fast about the XML documents that haven't the structure information. We first estract the feature of frequent structure from each XML document. And we cluster based on the similar structure by con-sidering the frequent structure as representative structure of the XML document, which makes it possible to retrieve the XML document raster than dealing with the whole documents that have different structure. And also we perform the structure retrieval about XML documents based on the clusters which is the group of similar structure. Moreover, we show efficiency of proposed method to describe how to apply the structure retrieval as well as to display the example of application result.

• Journal of Information Processing Systems
• /
• v.17 no.1
• /
• pp.28-36
• /
• 2021

This paper proposes two novel methods to model and simulate a mobile Internet of Things (IoT) system using the discrete event system specification (DEVS) formalism. In traditional simulation methods, it is advantageous to partition the simulation area hierarchically to reduce simulation time; however, in this case, the structure of the model may change as the IoT nodes to be modeled move. The proposed methods reduce the simulation time while maintaining the model structure, even when the IoT nodes move. To evaluate the performance of the proposed methods, a prototype mobile IoT system was modeled and simulated. The simulation results show that the proposed methods achieve good performance, even if the number of IoT nodes or the movement of IoT nodes increases.

• The korean journal of orthodontics
• /
• v.47 no.6
• /
• pp.353-364
• /
• 2017

Objective: The objective of this study was to investigate the effects of miniscrew-assisted rapid palatal expansion (MARPE) on changes in airflow in the upper airway (UA) of an adult patient with obstructive sleep apnea syndrome (OSAS) using computational fluid-structure interaction analysis. Methods: Three-dimensional UA models fabricated from cone beam computed tomography images obtained before (T0) and after (T1) MARPE in an adult patient with OSAS were used for computational fluid dynamics with fluid-structure interaction analysis. Seven and nine cross-sectional planes (interplane distance of 10 mm) in the nasal cavity (NC) and pharynx, respectively, were set along UA. Changes in the cross-sectional area and changes in airflow velocity and pressure, node displacement, and total resistance at maximum inspiration (MI), rest, and maximum expiration (ME) were investigated at each plane after MARPE. Results: The cross-sectional areas at most planes in NC and the upper half of the pharynx were significantly increased at T1. Moreover, airflow velocity decreased in the anterior NC at MI and ME and in the nasopharynx and oropharynx at MI. The decrease in velocity was greater in NC than in the pharynx. The airflow pressure in the anterior NC and entire pharynx exhibited a decrease at T1. The amount of node displacement in NC and the pharynx was insignificant at both T0 and T1. Absolute values for the total resistance at MI, rest, and ME were lower at T1 than at T0. Conclusions: MARPE improves airflow and decreases resistance in UA; therefore, it may be an effective treatment modality for adult patients with moderate OSAS.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.15 no.2
• /
• pp.144-152
• /
• 2011

The existing underground trenchless methods use reinforcing rod in steel tube to obtain structural stiffness. However, there are some problems such as inconvenience of work and expensive material fee which are resulted from limited working space and reinforcing work. To resolve these problems, a new trenchless method, namely P.S.T method (Prestressed Segment Tunnel Method), is developed which uses joint to connect the steel segment and form erection structure in underground construction. Further, installing strands for prestressing. In order to evaluate the flexural capacity of the P.S.T method structure, experiment was conducted. The parameters considered in the experiment are the span-to-depth ratio, diameter of steel tube at corner, prestressing force and welding of joint. Altogether examining the flexural behavior, the effect of deflection in structure according to different parameters has also been analysised.

• Korean Journal of Materials Research
• /
• v.29 no.6
• /
• pp.392-397
• /
• 2019

A cold roll-bonding process is applied to fabricate an AA6061/AA5052/AA6061 three-layer clad sheet. Two AA6061 and one AA5052 sheets of 2 mm thickness, 40 mm width, and 300 mm length are stacked, with the AA5052 sheet located in the center. After surface treatment such as degreasing and wire brushing, sample is reduced to a thickness of 1.5 mm by multi-pass cold rolling. The rolling is performed at ambient temperature without lubricant using a 2-high mill with a roll diameter of 400 mm at rolling speed of 6.0 m/sec. The roll bonded AA6061/AA5052/AA6061 complex sheet is then hardened by natural aging(T4) and artificial aging(T6) treatments. The microstructures of the as-roll bonded and age-hardened Al complex sheets are revealed by optical microscopy; the mechanical properties are investigated by tensile testing and hardness testing. After rolling, the roll-bonded AA6061/AA5052/AA6061 sheets show a typical deformation structure in which grains are elongated in the rolling direction. However, after T4 and T6 aging treatment, there is a recrystallization structure consisting of coarse equiaxed grains in both AA5052 and AA6061 sheets. The as roll-bonded specimen shows a sandwich structure in which an AA5052 sheet is inserted into two AA6061 sheets with higher hardness. However, after T4 and T6 aging treatment, there is a different sandwich structure in which the hardness of the upper and lower layers of the AA6061 sheets is higher than that of the center of the AA5052 sheet. The strength values of the T4 and T6 age-treated specimens are found to increase by 1.3 and 1.4 times, respectively, compared to that value of the starting material.