• Title/Summary/Keyword: T/T probe

Search Result 427, Processing Time 0.026 seconds

Detection of DNA Fragment to Differentiate Korean Cattle

  • Yeo, J.S.;Kim, J.W.;Chang, T.K.;Nam, D.H.;Han, J.Y.;Choi, C.B.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.15 no.8
    • /
    • pp.1071-1075
    • /
    • 2002
  • In order to identify and develop the specific DNA marker for the identification of Hanwoo (Korean Cattle) from other breeds, a specific DNA marker of 519 bp was identified and sequenced from polymorphic analysis using RAPD-PCR for 6 cattle breeds. Two different repetitive sequences, $(AAC)_5$ and $(GAAGA)_2$, were selected and designed to use specific probe to develop a DNA marker for Hanwoo specific. When the $(AAC)_5$ probe was applied, the 10 kb specific DNA marker showed in the DNA fingerprinting from 237 of 281 Hanwoo individuals. This novel Hanwoo specific DNA probe is useful to perform the marker-assisted selection for screening Hanwoo purity as an unique genetic source.

Fabrication and measurement of a Weathercock-Shaped Microstrip patch Antenna with T-Slot for 5.25-GHz Band Wireless LAN (5.25GHz 무선 LAN을 위한 T-Slot Weathercock-Shaped 마이크로스트립 패치 안테나 설계 및 제작)

  • Choi Sun-Ho;Jeong Gyey-Teak;Lee Hwa-Choon;Kwak Kyung-Sup
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.30 no.12A
    • /
    • pp.1183-1187
    • /
    • 2005
  • In this paper, a weathercock-shaped microstrip patch antenna for application in 5.25GHz band wireless LAN is designed and fabricated. To obtain sufficient bandwidth in VSWR<2, the T-slot is inserted on the patch, the coaxial probe source is used. The measured result of fabricated antenna obtained 350MHz or about $7.62\%$ bandwidth in VSWR<2 referenced to the center frequency, the gain of 5.25${\~}$6.70dBi. The experimental 3-dB beam width is shown to be broad across the pass band in azimuth and elevation at $80.32^{\circ}$ and $83.88^{\circ}$, in several.

Effect of Welding Condition on Tensile Properties of Friction Stir Welded Joints of Al-7075-T651 Plate (용접 조건이 Al-7075-T651의 마찰교반용접부의 인장 특성에 미치는 영향)

  • Kim, C.O.;Kim, S.J.
    • Journal of Power System Engineering
    • /
    • v.15 no.2
    • /
    • pp.61-68
    • /
    • 2011
  • This paper investigates the tensile properties of the friction stir welded joints of Al 7075-T651 aluminum plate according to the welding conditions. A 7075-T651 aluminum alloy plate with a thickness of 6.0 mm was used in this investigation. For the friction stir welding (FSW) process, a tool with shoulder diameter of 20 mm and probe diameter of 9 mm was used. The rotation speed and traverse speed conditions were changed in this study, the other welding conditions are constant. The welding direction was aligned with the material rolling direction, and dimension of the FSW plate were $250{\times}100{\times}6\;mm$. As far as this work is concerned, the optimal FSW conditions are determined as the rotation speed, 600 rpm and traverse speed 0.8 mm/sec or the rotation speed, 800 rpm and traverse speed 0.5 mm/sec.

Investigation of High Temperature Electrical Conductivity of CaO-partially Stabilized $ZrO_2$ (CaO에 의하여 부분 안정화된 $ZrO_2$의 고온 전기 전도도에 대한 연구)

  • 변수일
    • Journal of the Korean Ceramic Society
    • /
    • v.16 no.4
    • /
    • pp.213-224
    • /
    • 1979
  • The present work was undertaken: (1) to determine if CaO-partially stabilized $ZrO_2$ prepared by Hot Petroleum Drying Method would show better ionic conductor as an oxygen sensor in molten metals than that prepared by Oxide Wet Mixing Method and than CaO-fully stabilized $ZrO_2$, and (2) to understand the nature of conduction mechanism of CaO-partially stabilized $ZrO_2$ by a comparison of measured electrical conductivity data with theory on defect structure of pure monoclinic $ZrO_2$ and fully stabilized cubic $ZrO_2$. The DC electrical conductivity was measured by 3-probe technique and the AC electrical conductivity by 2-probe technique as a function of temperature in the range 973-1373 K and oxygen partial pressure in the range 10-1-10-25Mpa. The results of the experiments were as follows: 1. CaO-partially stabilized $ZrO_2$ prepared by Hot petroleum Drying Method showed at T=1094-1285 K and $Po_2$=10-7-10-25 MPa a nearly ionic conduction with 4 times higher conductivity than that prepared by Oxide Wet Mixing Method. 2. High-oxygen pressure conductivity tends toward a Po_2^{+1/5}-Po_2^{+1/6}$dependence. An analysis of possible defect structures suggests that CaO-partially stabilized $ZrO_2$ has an anti-Frenkel defect in which singly or doubly ionized oxygen interstitials and defect electrons predominate at T=1094-1285 K and $Po_2$=10-1-10-7MPa. 3. The activation energy for pure electron hole-conduction and ionic conduction of CaO-partially stabilized $ZrO_2$ was found to be 130 KJ/mol at T=973-1373 K, $Po_2$=2, 127 10-2 MPa(air) and 153KJ/mol at T=1094-1285 K respectively.

  • PDF

The Preparation Characteristic of Dimercaptan-Polyphenylenediamine Cathodes for Lithium Battery (리튬전지용 Dimercaptan-Polyphenylenediamine 정극의 제막특성)

  • Park, Soo-Gil;Lee, Ju-Seong
    • Applied Chemistry for Engineering
    • /
    • v.8 no.1
    • /
    • pp.114-121
    • /
    • 1997
  • The positive active material for polymer film-battery was prepared by using polyphenlenediamine(PPD) synthesized in our lab. and 2,5-dimercapto-1,3,4-thiadiazole(DMcT) with various mixture ratio. The transference measurement of surface morphology and thermal stability of the prepared composite film was carried out by using SEM and TGA, respectively. Electrochemical property and electrical conductivity of the composite film were also measured by using cyclic voltammetry and four-probe method in dry box, respectively. The thermal stability of prepared composite film was up to $200^{\circ}C$. The electrical conductivity of the composite film increased and showed the highest value(about 3 S/cm) when doped at 0.4% $LiCIO_4$ solution. And we could confirm that DMcT was effective on reactivation of PPD through cyclic voltammogram.

  • PDF

The Coordination Chemistry of DNA Nucleosides on Gold Nanoparticles as a Probe by SERS

  • Jang, Nak-Han
    • Bulletin of the Korean Chemical Society
    • /
    • v.23 no.12
    • /
    • pp.1790-1800
    • /
    • 2002
  • The DNA nucleosides(dA, dC, dG, dT)bound to gold nanoparticles (~13 nm) in aqueous solution has been studied as a probe by the SERS and their coordination structures have been proposed on the basis of them. According to UV-Visible absorption of gold nanoparticles after modifying with DNA nucleosides, the rates of absorption of dA, dC, and dG were much faster than that of dT as monitored by the aggregation kinetics at 700 nm. These data indicated that the nucleosides dA, dC, and dG had a higher affinity for the gold nanoparticles surface than nucleoside dT. As the result of SERS spectra, the binding modes of each of the nucleosides on gold nanoparticles have been assigned. A dA binds to gold nanoparticles via a N(7) nitrogen atom of the imidazole ring, which the C(6)-$NH_2$ group also participates in the coordination process. In the case of dC, it binds to the gold surface via a N(3) nitrogen atom of the pyrimidine ring with a partial contribution from the oxygen of C(2)=O group. A coordination of dG to the gold surfaces is also proposed. Although the dG has the two different nitrogens of a pyrimidine ring and the amino group, the N(1) nitrogen atom of a pyrimidine ring has a higher affinity after the hydrogen migrates to the amino group. Conversely, dT binds via the oxygen of the C(4)=O group of the pyrimidine ring. Accordingly, these data suggest that the nitrogen atom of the imidazole or the pyrimidine ring in the DNA nucleosides will bind more fast to the gold nanoparticles surfaces than the oxygen atom of the carbonyl group.

Fiber Bragg Grating Temperature Sensor by the Wavelength Tuning Using the Temperature Dependence of VCSEL (빅셀(VCSEL)의 온도 의존성을 이용한 파장 가변 형 광섬유 격자 온도센서)

  • Lee, Chung-Ki;Kim, Sung-Moon
    • Korean Journal of Optics and Photonics
    • /
    • v.29 no.6
    • /
    • pp.241-246
    • /
    • 2018
  • In this paper, a low-cost optical temperature sensor is implemented, using a fiber Bragg grating (FBG) as the temperature probe and a low-cost VCSEL with temperature-dependent output wavelength as the light source. To analyze the wavelength of the reflected light from the FBG, an interrogation was applied using a method of referring to the internal temperature according to the output wavelength of the VCSEL. When the temperature of the VCSEL was adjusted from 14 to $52.2^{\circ}C$, the output wavelength varied from 1519.90 to 1524.25 nm. The degree of wavelength tuning according to temperature was $0.114nm/^{\circ}C$. The variable wavelength repeatability error according to temperature was ${\pm}0.003nm$, and the temperature measurement error was ${\pm}0.18^{\circ}C$. As a result of measuring the temperatures from 22.3 to $194.2^{\circ}C$, the value of the internal temperature change of the light source according to the applied temperature ${\Delta}T$ was $0.146^{\circ}C/{\Delta}T$, the change in reflected wavelength of the temperature probe according to applied temperature ${\Delta}T$ was measured at $16.64pm/^{\circ}C$. and the temperature measurement error of the sensor was ${\pm}1^{\circ}C$.

Cloning of a Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) from a Scylliorhinus torazame (두툽상어에서 tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) 유전자의 클로닝)

  • Kim, Cha-Soon;Bae, Su-Kyoung;Kim, Kyu-Won;Kim, Yung-Jin
    • Journal of Life Science
    • /
    • v.6 no.4
    • /
    • pp.286-292
    • /
    • 1996
  • Angiogenesis is a fundamental process by which new blood vessels are formed. It is essential in embryo development, and wound healing. Furthermore, malignant tumor growth and metastasis are also angiogenesis-dependent. In the catilage tissue, normal angiogenesis process is suppressed. In fact, it was reported that angiogenesis-inhibitory substances were isolated from the extracts of cow and shark catilage tissue. In order to isolate genes involved in the regulation of angiogenesis from a catilage fish, we constructed a shark cDNA library from Scylliohinus torazame. We then screened the library using hyman tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) gene as a probe. Among the 4 X 10$^{4}$ plaques screened, we isolated 2 positive clones (T-1, T-2). Restriction enzyme analysis revealed that the T-1 clone contains 0.8 kb cDNA insert, and the T-2 clone contains 1.2 kb and 2.2 kb inserts, respectively. Further DNA sequence analysis shows that the DNA sequence of the T-1 clone is 53% homologous to that of the human TIMP-1 gene.

  • PDF

An automated measurement system for the microwave surface resistance of high-T$_c$ superconductor films

  • Lee, J.H.;Lim, J.;Lee, Jung-Hun;Lee, Sang-Young
    • 한국초전도학회:학술대회논문집
    • /
    • v.10
    • /
    • pp.173-178
    • /
    • 2000
  • A prototype for a highly sensitive, automated measurement system for the microwave surface resistance of high-T$_c$ superconductor films was set up, and tested by measuring the microwave surface resistances of high-T$_c$ YBa$_2$Cu$_3$O$_{7-{\delta}}$(YBCO) films at the frequency of about 19.6 GHz and the temperature of 30 K ${\sim}$ 90 K. An open-ended TE$_{011}$ mode sapphire-loaded cylindrical cavity resonator was used as the measurement probe, where YBCO films were used as the endplates of the cylindrical cavity. The characteristics of the measurement system include functions to display the unloaded ${\varrho}$ and the resonant frequency of the TE$_{011}$ mode resonator as well as the microwave surface resistance of the YBCO films, all simultaneously as a function of temperature. Applicability of the measurement system for investigating the homogeneity in the microwave properties of large high-T$_c$ superconductor films is discussed.

  • PDF

An Automated Measurement System for the Microwave Surface Resistance of High-$T_c$ Superconductor Films

  • Lee, J.H.;Lim, J.;Lee, Jung-Hun;Lee, Sang-Young
    • Progress in Superconductivity
    • /
    • v.2 no.1
    • /
    • pp.27-32
    • /
    • 2000
  • A prototype for a highly sensitive, automated measurement system for the microwave surface resistance of high-$T_c$ superconductor films was set up, and tested by measuring the microwave surface resistances of high-$T_c$ $YBa_2Cu_3O_{7-\delta}$ (YBCO) films at the frequency of about 19.6 GHz and the temperature of 30 K $\sim$ 90 K. An open-ended $TE_{011}$ mode sapphire-loaded cylindrical cavity resonator was used as the measurement probe, where YBCO films were used as the endplates of the cylindrical cavity. The characteristics of the measurement system include functions to display the unloaded Q and the resonant frequency of the $TE_{011}$ mode resonator as well as the microwave surface resistance of the YBCO films, all simultaneously as a function of temperature. Applicability of the measurement system for investigating the homogeneity in the microwave properties of large high-$T_c$ superconductor films is discussed.

  • PDF